FastQCFastQC Report
Wed 25 May 2016
SRR1032946_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032946_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2644855
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48170.1821271865565409No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40420.15282501309145494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37950.045.8009341
GTATCAA68650.043.1790661
ATCAACG79750.036.212993
TCAACGC79750.036.095034
CAACGCA82150.034.978635
TATCAAC85650.033.9374772
AACGCAG84850.033.8095136
ACGCAGA100700.028.347857
GTACATG82200.027.6603661
CGCAGAG102800.027.6315178
TACATGG82750.025.9756662
GTGGTAT19300.025.8007871
GCAGAGT113000.025.1064579
ACATGGG86150.024.8963813
GAGTACA59700.022.032721
CGCGCGT1100.003049849321.3765817
AGAGTAC102000.020.95221510-11
TATAACG2257.945346E-820.9043162
ACACCGT5050.020.4876546
TACACCG4200.020.1554455
Produced by FastQC (version 0.11.5)