FastQCFastQC Report
Wed 25 May 2016
SRR1032945_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032945_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2145228
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG67700.029.1598821
TACATGG69350.027.6527822
ACATGGG69400.026.4814913
GTATCAA47650.021.79991
TCAACGC46450.020.5416564
GAGTACA47300.020.4706291
ATCAACG47850.020.0388763
CAACGCA47850.019.940185
CATGGGG47200.019.8169234
AACGCAG48950.019.3951636
TATCAAC53450.018.818772
GGTATCA35000.018.801181
TATAACG1250.006410613718.801182
CATGGGA23800.018.7616834
TATACTG12850.018.288665
AGTACAT49500.018.2314472
GTACCTG19050.017.2714251
TACCTGG16050.016.985492
GTCTAGG7750.016.9817121
ACGCAGA55100.016.7185737
Produced by FastQC (version 0.11.5)