FastQCFastQC Report
Wed 25 May 2016
SRR1032945_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032945_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2145228
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49740.2318634662609289No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43420.20240272828808875No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22330.10409149983125338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56350.033.3515471
GTATCAA86550.031.1055241
TCAACGC94050.027.9699694
ATCAACG94800.027.649413
CAACGCA95900.027.4265585
AACGCAG97850.027.0242486
GTACATG61350.026.7276521
TATCAAC100850.026.1300932
TACATGG62150.024.5319842
ACGCAGA110100.023.887587
CGCAGAG111950.023.4928348
ACATGGG64550.023.0372073
ACCGTTC1903.6066558E-722.2862328
GAGTACT69650.022.05390412-13
GCAGAGT121800.021.1735679
GTACTTT76650.019.82535614-15
AGAGTAC94450.019.7942910-11
CTGTCGC5000.019.7295999
CGCGTAT600.005082494619.57255712-13
CATGGGG43050.019.4574454
Produced by FastQC (version 0.11.5)