FastQCFastQC Report
Wed 25 May 2016
SRR1032944_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032944_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4133638
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40450.033.5805971
GTATCAA60050.033.421351
TCAACGC58300.032.732344
ATCAACG60200.031.7769553
CAACGCA60600.031.4117055
AACGCAG64600.029.5391066
GTACATG125300.027.1204151
TATCAAC72200.026.8857382
TACATGG123800.025.81652
ACGCAGA74500.025.2983367
CGCAGAG74950.025.0210258
ACATGGG129200.023.7191683
CATGGGG82350.019.0634924
GCAGAGT93850.019.030639
GAGTACA95800.018.643491
AGTACAT95750.017.2787762
GTCGTAC2807.977105E-716.786151
TCGCGTA2304.6802277E-516.3480519
GTATTAG21450.016.2148721
CAGAGTA92900.015.88592510-11
Produced by FastQC (version 0.11.5)