FastQCFastQC Report
Wed 25 May 2016
SRR1032944_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032944_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4133638
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82980.2007432677946158No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68280.16518137292138307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60500.052.1854361
GTATCAA114350.043.181921
TCAACGC135650.035.8703424
ATCAACG136000.035.847233
CAACGCA138550.035.1492425
AACGCAG144150.033.7511066
TATCAAC147850.033.1006282
ACGCAGA166250.029.1502277
CGCAGAG169050.028.5839088
GTACATG117400.027.7332341
TACATGG117450.026.3230722
GCAGAGT188800.025.3041239
ACATGGG121400.024.5756683
GTGGTAT31250.021.64951
AGAGTAC172200.021.04576110-11
GAGTACT129550.020.99450512-13
CAGAGTA183700.020.66160410-11
CATGGGG76400.020.1413764
TGGTATC33000.019.2505912
AGTACTT136750.019.13340812-13
Produced by FastQC (version 0.11.5)