##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032944_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4133638 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.84984993848034 34.0 31.0 34.0 31.0 34.0 2 32.91024734144596 34.0 31.0 34.0 31.0 34.0 3 32.98997880317531 34.0 33.0 34.0 31.0 34.0 4 36.387540708692924 37.0 37.0 37.0 35.0 37.0 5 36.37124949015855 37.0 37.0 37.0 35.0 37.0 6 36.42926158507349 37.0 37.0 37.0 35.0 37.0 7 36.39323230529621 37.0 37.0 37.0 35.0 37.0 8 36.33618763907241 37.0 37.0 37.0 35.0 37.0 9 38.18158508316403 39.0 39.0 39.0 37.0 39.0 10-11 38.19556562524343 39.0 39.0 39.0 37.0 39.0 12-13 38.18530843774902 39.0 39.0 39.0 37.0 39.0 14-15 39.74768351752137 41.0 40.0 41.0 37.0 41.0 16-17 39.76116982183733 41.0 40.0 41.0 37.0 41.0 18-19 39.78573619654165 41.0 40.0 41.0 37.5 41.0 20-21 39.79804037025013 41.0 40.0 41.0 37.0 41.0 22-23 39.76291731399798 41.0 40.0 41.0 37.0 41.0 24-25 39.744416056751945 41.0 40.0 41.0 37.0 41.0 26-27 39.66818635303817 41.0 40.0 41.0 37.0 41.0 28-29 39.60852135576458 41.0 40.0 41.0 37.0 41.0 30-31 39.499136353981655 41.0 40.0 41.0 36.5 41.0 32-33 39.37367072298058 41.0 39.5 41.0 36.0 41.0 34-35 39.282080941775746 41.0 39.0 41.0 35.0 41.0 36-37 39.19175614797426 41.0 39.0 41.0 35.0 41.0 38-39 39.096247300803796 41.0 39.0 41.0 35.0 41.0 40-41 38.99411245009844 41.0 39.0 41.0 35.0 41.0 42-43 38.860001166043084 41.0 39.0 41.0 35.0 41.0 44-45 38.691285860058386 41.0 39.0 41.0 35.0 41.0 46-47 38.472487552127205 41.0 39.0 41.0 35.0 41.0 48-49 38.27478422155012 41.0 38.0 41.0 34.5 41.0 50-51 38.128169665558524 41.0 38.0 41.0 34.0 41.0 52-53 37.99162589951031 40.0 38.0 41.0 34.0 41.0 54-55 37.85332774664835 40.0 38.0 41.0 34.0 41.0 56-57 37.652746926557185 40.0 37.0 41.0 33.5 41.0 58-59 37.49136329789884 40.0 37.0 41.0 33.0 41.0 60-61 37.305653276847174 40.0 36.5 41.0 33.0 41.0 62-63 37.07465155390966 39.5 36.0 41.0 33.0 41.0 64-65 36.79196267307394 39.0 35.0 41.0 32.5 41.0 66-67 36.523384244096846 39.0 35.0 41.0 32.0 41.0 68-69 36.23317450633074 38.0 35.0 40.5 32.0 41.0 70-71 35.911079901046 37.0 35.0 40.0 31.5 41.0 72-73 35.56773682165685 37.0 35.0 39.5 31.0 41.0 74-75 35.221945414668625 36.0 35.0 39.0 31.0 41.0 76-77 34.86729062873914 36.0 35.0 39.0 31.0 40.5 78-79 34.5175699952439 35.5 35.0 37.5 31.0 39.5 80-81 34.17935351378132 35.0 35.0 37.0 30.5 39.0 82-83 33.86277789685502 35.0 34.0 37.0 30.0 39.0 84-85 33.57517784576201 35.0 34.0 36.0 30.0 38.0 86-87 33.42046461252775 35.0 34.0 36.0 30.0 37.0 88-89 33.22144924156397 35.0 34.0 36.0 30.0 37.0 90-91 33.04189590380193 35.0 34.0 35.0 30.0 36.5 92-93 32.87658583068958 35.0 34.0 35.0 30.0 36.0 94-95 32.77548796000037 35.0 34.0 35.0 30.0 36.0 96-97 32.711130123150596 35.0 34.0 35.0 30.0 36.0 98-99 32.599840261774254 35.0 34.0 35.0 30.0 36.0 100 32.51434523294009 35.0 34.0 35.0 30.0 36.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19369800789721126 1101 2 0.20817720981020216 1101 3 0.21656329611686687 1101 4 0.1353777048166407 1101 5 0.14577510619066203 1101 6 0.11652065839282955 1101 7 0.12797330439212118 1101 8 0.016061912342074436 1101 9 0.10246758586922766 1101 10-11 0.17400539914435598 1101 12-13 0.21741823970857865 1101 14-15 0.2793809411706434 1101 16-17 0.2627948984146755 1101 18-19 0.262221472397421 1101 20-21 0.29781694768414013 1101 22-23 0.2628673557438219 1101 24-25 0.28079791271414223 1101 26-27 0.30295370140558475 1101 28-29 0.3149340543555681 1101 30-31 0.3227984592878812 1101 32-33 0.29690924157674203 1101 34-35 0.31515613361274575 1101 36-37 0.3094867718368661 1101 38-39 0.27241258126655765 1101 40-41 0.32857753302010195 1101 42-43 0.33681301622502957 1101 44-45 0.3536211902493278 1101 46-47 0.3561309940859445 1101 48-49 0.3803522069011933 1101 50-51 0.38805335284541087 1101 52-53 0.4213429826684276 1101 54-55 0.3720026913555259 1101 56-57 0.3651971308819313 1101 58-59 0.3274930934391733 1101 60-61 0.3669574393458319 1101 62-63 0.38121309148286997 1101 64-65 0.41134740971186545 1101 66-67 0.4265763752918872 1101 68-69 0.3479824834260583 1101 70-71 0.3416537261060597 1101 72-73 0.3602957556003119 1101 74-75 0.3688078979447482 1101 76-77 0.3669573914303399 1101 78-79 0.3176035157678285 1101 80-81 0.32499122587370977 1101 82-83 0.3399172910147783 1101 84-85 0.39159554307572364 1101 86-87 0.37325171335717044 1101 88-89 0.34908171888633177 1101 90-91 0.3162830512983348 1101 92-93 0.3429194815589014 1101 94-95 0.3627013219341251 1101 96-97 0.3136232522475595 1101 98-99 0.34795394336526897 1101 100 0.3690849824991673 1102 1 0.1786958153615572 1102 2 0.20745190195027163 1102 3 0.1785598402876687 1102 4 0.12454824634192363 1102 5 0.10242146259974305 1102 6 0.11424532090264705 1102 7 0.10132901899844171 1102 8 0.034353490109346296 1102 9 0.10671665791988261 1102 10-11 0.18409125708252105 1102 12-13 0.1557522047748634 1102 14-15 0.21545146221846068 1102 16-17 0.20949637330858906 1102 18-19 0.22926576110058505 1102 20-21 0.222026413181176 1102 22-23 0.22273183351182269 1102 24-25 0.2177586989036513 1102 26-27 0.22079460062444412 1102 28-29 0.2090022952180206 1102 30-31 0.21375888427782286 1102 32-33 0.25251159033057036 1102 34-35 0.20799957312203077 1102 36-37 0.2580067197744782 1102 38-39 0.30642897693443416 1102 40-41 0.23287690669953776 1102 42-43 0.2206567820291241 1102 44-45 0.2596640563898518 1102 46-47 0.23642226391572052 1102 48-49 0.24380884925001567 1102 50-51 0.2895247627492594 1102 52-53 0.2687583696664859 1102 54-55 0.1974098553106458 1102 56-57 0.1813463259524397 1102 58-59 0.21830940704209922 1102 60-61 0.214071417329464 1102 62-63 0.21868928514788877 1102 64-65 0.2680263891382282 1102 66-67 0.2624267507636162 1102 68-69 0.21519274423437906 1102 70-71 0.2458280244170865 1102 72-73 0.2645928992537989 1102 74-75 0.26425300207468183 1102 76-77 0.32569813003561165 1102 78-79 0.3456708809087843 1102 80-81 0.31400211046877047 1102 82-83 0.27266668936024274 1102 84-85 0.31276212200648956 1102 86-87 0.29160826567758846 1102 88-89 0.21644426784348525 1102 90-91 0.24181265983764177 1102 92-93 0.21439781811726988 1102 94-95 0.26692949637929786 1102 96-97 0.25231767353708534 1102 98-99 0.2887368001151174 1102 100 0.2980167703361829 1103 1 0.0959040687063677 1103 2 0.14251753423673819 1103 3 0.10017072102442626 1103 4 0.04158419771384558 1103 5 0.08125093109294568 1103 6 0.055923419795945506 1103 7 0.10257448610977349 1103 8 -0.023336101677685406 1103 9 0.019696068544583056 1103 10-11 0.08040673785568941 1103 12-13 0.09026281342401887 1103 14-15 0.09136475843242664 1103 16-17 0.15110064615040386 1103 18-19 0.1728887285520031 1103 20-21 0.15341547721387627 1103 22-23 0.19375652922183662 1103 24-25 0.15049321955758188 1103 26-27 0.1576186211875097 1103 28-29 0.14814340355277267 1103 30-31 0.2009160240477712 1103 32-33 0.23366676478306658 1103 34-35 0.19634182180055149 1103 36-37 0.1517297632265766 1103 38-39 0.12790340612314566 1103 40-41 0.12664409133472532 1103 42-43 0.14735897292685962 1103 44-45 0.131490107632672 1103 46-47 0.18556077876291255 1103 48-49 0.17362146452213523 1103 50-51 0.12929674712803063 1103 52-53 0.13375433734315578 1103 54-55 0.14736549172272362 1103 56-57 0.16688537783318935 1103 58-59 0.14725773238924234 1103 60-61 0.14964206305349848 1103 62-63 0.13208091696804303 1103 64-65 0.1420647507608379 1103 66-67 0.14669039119232252 1103 68-69 0.14788633621960656 1103 70-71 0.11267498727509917 1103 72-73 0.10457595200489322 1103 74-75 0.07680621706639812 1103 76-77 0.13460582078267436 1103 78-79 0.16191783875034105 1103 80-81 0.13627302699541843 1103 82-83 0.13828678062282762 1103 84-85 0.15625645296886148 1103 86-87 0.1484129086010526 1103 88-89 0.14030344529894734 1103 90-91 0.15343796476394544 1103 92-93 0.20779152942268553 1103 94-95 0.2580011775832034 1103 96-97 0.24186918676109315 1103 98-99 0.26115140252507274 1103 100 0.23735924772100958 1104 1 0.10790111440594075 1104 2 0.13532383402640846 1104 3 0.15829771967776196 1104 4 0.05097027611823535 1104 5 0.07661682264684089 1104 6 0.10649110459882394 1104 7 0.05608265268595147 1104 8 0.022174974637906075 1104 9 0.05055984469469621 1104 10-11 0.06901162138729688 1104 12-13 0.10863122492696675 1104 14-15 0.18192782003009 1104 16-17 0.14546035948560387 1104 18-19 0.1515901044562824 1104 20-21 0.15043717783520805 1104 22-23 0.16318402922213693 1104 24-25 0.18297905810283766 1104 26-27 0.18084519059738824 1104 28-29 0.1973545254604474 1104 30-31 0.2060598879783413 1104 32-33 0.2278013324090864 1104 34-35 0.20326661525253797 1104 36-37 0.26768075156261517 1104 38-39 0.266549964869192 1104 40-41 0.19780918645530932 1104 42-43 0.24763115065325536 1104 44-45 0.21091978946347467 1104 46-47 0.22462600364374197 1104 48-49 0.2279088977591357 1104 50-51 0.22610866136846397 1104 52-53 0.25085857894728747 1104 54-55 0.23693609302027596 1104 56-57 0.22385106431161006 1104 58-59 0.24863943196291416 1104 60-61 0.2424549521846373 1104 62-63 0.20821139493587992 1104 64-65 0.20066109185123082 1104 66-67 0.21421902312533803 1104 68-69 0.21495454384406543 1104 70-71 0.21346560158779226 1104 72-73 0.24539459353630377 1104 74-75 0.23375357545748443 1104 76-77 0.2638761969878516 1104 78-79 0.29591823031840647 1104 80-81 0.2787191529070654 1104 82-83 0.2592753856841341 1104 84-85 0.3010539723425367 1104 86-87 0.273950085108531 1104 88-89 0.24367071729618317 1104 90-91 0.22699305906559886 1104 92-93 0.2931792025425253 1104 94-95 0.26213990028676903 1104 96-97 0.21830746163831094 1104 98-99 0.23414784274001477 1104 100 0.21008962549767318 1105 1 0.028042536385349592 1105 2 0.09464326613822038 1105 3 0.08683127622740017 1105 4 0.056816192213872796 1105 5 0.03890579926188309 1105 6 0.03934297027705469 1105 7 0.05893033616140997 1105 8 8.045841934816167E-4 1105 9 0.0447560439959247 1105 10-11 0.041614074777008625 1105 12-13 0.06265507606379117 1105 14-15 0.09343954895655315 1105 16-17 0.1311700524415116 1105 18-19 0.06974898573593435 1105 20-21 0.09138991048131118 1105 22-23 0.09906892279317248 1105 24-25 0.11390475791094445 1105 26-27 0.12703576127314875 1105 28-29 0.08566351978031861 1105 30-31 0.136094803419482 1105 32-33 0.11425022533268248 1105 34-35 0.1104308391791804 1105 36-37 0.11738675337056037 1105 38-39 0.1402022792216755 1105 40-41 0.18535623059478468 1105 42-43 0.13025562421784542 1105 44-45 0.07800789876649361 1105 46-47 0.10949817847775734 1105 48-49 0.21580406387630546 1105 50-51 0.27066068640819907 1105 52-53 0.2224265817166895 1105 54-55 0.22509787244855062 1105 56-57 0.3033543130645526 1105 58-59 0.29341234421976026 1105 60-61 0.2544012397927915 1105 62-63 0.1950765673870123 1105 64-65 0.1934616579859636 1105 66-67 0.2561947452492461 1105 68-69 0.2585511868886883 1105 70-71 0.20925107179280644 1105 72-73 0.24037315756092426 1105 74-75 0.22428635582006962 1105 76-77 0.2069916525825093 1105 78-79 0.22371569830576732 1105 80-81 0.2726843099055358 1105 82-83 0.2660829037648469 1105 84-85 0.2553701347988593 1105 86-87 0.20406138936059648 1105 88-89 0.21566569910419275 1105 90-91 0.20257091023706408 1105 92-93 0.2101636199585144 1105 94-95 0.2542614579031479 1105 96-97 0.2604828299251878 1105 98-99 0.24752684368156253 1105 100 0.2049445513545436 1106 1 0.10707049975109584 1106 2 0.14941203707444117 1106 3 0.11686555987951408 1106 4 0.04758282495993171 1106 5 0.0834130184984403 1106 6 0.08691333927470879 1106 7 0.07062107778719451 1106 8 0.06933298267496468 1106 9 0.11312375309802292 1106 10-11 0.14059917951364298 1106 12-13 0.09199950078443919 1106 14-15 0.14623982758234888 1106 16-17 0.10446466155802625 1106 18-19 0.12277450313352034 1106 20-21 0.12828639736877534 1106 22-23 0.1861205856071919 1106 24-25 0.18559602243421836 1106 26-27 0.18591271834570477 1106 28-29 0.14909664796443423 1106 30-31 0.16490351559610872 1106 32-33 0.1643913534691137 1106 34-35 0.21720409565470078 1106 36-37 0.2185101430901213 1106 38-39 0.1964330227764961 1106 40-41 0.18626489367865418 1106 42-43 0.24842003998691808 1106 44-45 0.23907918297022235 1106 46-47 0.2602572874604334 1106 48-49 0.22159024973737473 1106 50-51 0.23848292943795002 1106 52-53 0.2212940345177401 1106 54-55 0.23532623505871442 1106 56-57 0.2632078828017441 1106 58-59 0.19251481641407509 1106 60-61 0.18190306045141824 1106 62-63 0.25737057828711585 1106 64-65 0.20649582622132812 1106 66-67 0.20249078867628612 1106 68-69 0.2766790811127535 1106 70-71 0.29611050881914736 1106 72-73 0.274582303552819 1106 74-75 0.24717317262604865 1106 76-77 0.28498281588583296 1106 78-79 0.2579870501339556 1106 80-81 0.23879341341539373 1106 82-83 0.23317173564977622 1106 84-85 0.26759499047373225 1106 86-87 0.22248783637780178 1106 88-89 0.22244022895702642 1106 90-91 0.27133959772643124 1106 92-93 0.2661891465677684 1106 94-95 0.22225502436747746 1106 96-97 0.2059522076392497 1106 98-99 0.208093951749575 1106 100 0.22035144146370556 1107 1 0.031059616929070444 1107 2 0.041234284314647596 1107 3 -0.00535275328307705 1107 4 0.011556149536971816 1107 5 -0.00415851729291461 1107 6 0.005511863213790491 1107 7 0.010713957044160338 1107 8 0.034267154110686704 1107 9 0.016051638765851806 1107 10-11 -7.285716825293775E-4 1107 12-13 0.018257827081463063 1107 14-15 0.030854941570524375 1107 16-17 0.0682210333252371 1107 18-19 0.08004926311726734 1107 20-21 0.04106511332086882 1107 22-23 0.041818821839491704 1107 24-25 0.045223645413237534 1107 26-27 0.05064049143260263 1107 28-29 0.038671185913592865 1107 30-31 0.08729890916023919 1107 32-33 0.0720570194165191 1107 34-35 0.04607875556651919 1107 36-37 0.07048812726625187 1107 38-39 0.06966769144460017 1107 40-41 0.061669481351039224 1107 42-43 0.04090330921167151 1107 44-45 0.08628029627040235 1107 46-47 0.04701378431754932 1107 48-49 0.08482515294905113 1107 50-51 0.10665380225278653 1107 52-53 0.11088499051736989 1107 54-55 0.10105941828415865 1107 56-57 0.16579919843484703 1107 58-59 0.07922440455554636 1107 60-61 0.03808914732636026 1107 62-63 0.09585018863204908 1107 64-65 0.1255841553328736 1107 66-67 0.05187472087755651 1107 68-69 0.054354832434015066 1107 70-71 0.04229331208399856 1107 72-73 0.01083179537506851 1107 74-75 -8.707339317908236E-4 1107 76-77 0.04186901534169607 1107 78-79 -0.011440976260018942 1107 80-81 0.0033026700686136223 1107 82-83 0.0203891106380496 1107 84-85 0.06586249524278287 1107 86-87 0.05315910009860403 1107 88-89 0.08132161245141845 1107 90-91 0.08854493281102549 1107 92-93 0.038103725796588606 1107 94-95 0.021633874791220364 1107 96-97 -0.002578924111702463 1107 98-99 0.02477860009056343 1107 100 0.04995709552756722 1108 1 0.042889299632861366 1108 2 0.07063682933640791 1108 3 0.07489467604859357 1108 4 6.941741897463771E-4 1108 5 -0.028687311583340147 1108 6 0.024216480799324813 1108 7 0.05422537377695136 1108 8 0.04636191820227964 1108 9 0.016686090930981834 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-0.19456025237156638 2214 26-27 -0.18875180644596412 2214 28-29 -0.20791039116245003 2214 30-31 -0.1785504798349109 2214 32-33 -0.16529359447740433 2214 34-35 -0.21437086862495391 2214 36-37 -0.21540816967206666 2214 38-39 -0.1396488931791353 2214 40-41 -0.13442111847837168 2214 42-43 -0.10471988553180012 2214 44-45 -0.05796093951489212 2214 46-47 -0.1191218849578135 2214 48-49 -0.05471186092437108 2214 50-51 -0.05932191776509654 2214 52-53 -0.07767391000877666 2214 54-55 -0.012520050429536411 2214 56-57 -0.048907400391264844 2214 58-59 -0.04057483154885233 2214 60-61 -0.08439156979260787 2214 62-63 -0.07991657961158438 2214 64-65 -0.11600586898124732 2214 66-67 -0.1208030437462071 2214 68-69 -0.15571425566479746 2214 70-71 -0.11030573202877747 2214 72-73 -0.12508121218968427 2214 74-75 -0.1295690516411767 2214 76-77 -0.11179872093638465 2214 78-79 -0.17446948675553386 2214 80-81 -0.1630660287284158 2214 82-83 -0.1874189213623083 2214 84-85 -0.1908230357184948 2214 86-87 -0.13352387666476062 2214 88-89 -0.15937818524822944 2214 90-91 -0.18165936362563428 2214 92-93 -0.18123828261716568 2214 94-95 -0.16177956449208608 2214 96-97 -0.18420337428613465 2214 98-99 -0.17879816340580845 2214 100 -0.1684887439860745 2215 1 -0.160398956511834 2215 2 -0.16994512406030537 2215 3 -0.173872759336696 2215 4 -0.08194202855590049 2215 5 -0.09655074537472785 2215 6 -0.12660841758109598 2215 7 -0.14856845602234614 2215 8 -0.060271781150490256 2215 9 -0.12576025659964074 2215 10-11 -0.1795263458126115 2215 12-13 -0.16198175661567404 2215 14-15 -0.24085194675181043 2215 16-17 -0.2366725044342317 2215 18-19 -0.23434380315824654 2215 20-21 -0.2508899734671033 2215 22-23 -0.2545053495711258 2215 24-25 -0.2704785164279926 2215 26-27 -0.27509321908788564 2215 28-29 -0.2395410167895804 2215 30-31 -0.25099955764380155 2215 32-33 -0.29758814525362 2215 34-35 -0.271993049693144 2215 36-37 -0.2893321267530027 2215 38-39 -0.2783631354567646 2215 40-41 -0.24392022283643655 2215 42-43 -0.27420222170254505 2215 44-45 -0.205474555357668 2215 46-47 -0.16417493732065225 2215 48-49 -0.1946134901084875 2215 50-51 -0.16365784281875762 2215 52-53 -0.19014994586139977 2215 54-55 -0.18751553602263726 2215 56-57 -0.2526003172754585 2215 58-59 -0.21405956848255414 2215 60-61 -0.13234531640430447 2215 62-63 -0.15847058343747022 2215 64-65 -0.14649235312472797 2215 66-67 -0.1838602702166341 2215 68-69 -0.18093370410307585 2215 70-71 -0.21616895173747253 2215 72-73 -0.22446896482004064 2215 74-75 -0.21782611175923705 2215 76-77 -0.22274582301498214 2215 78-79 -0.2536841921298105 2215 80-81 -0.2543126688759614 2215 82-83 -0.2830707666597121 2215 84-85 -0.3035050177666818 2215 86-87 -0.3566920041051631 2215 88-89 -0.34800119689444386 2215 90-91 -0.35284611947130884 2215 92-93 -0.36545452284425295 2215 94-95 -0.4046778401297857 2215 96-97 -0.3675325324191334 2215 98-99 -0.415436652706326 2215 100 -0.39034721534915917 2216 1 -0.21180066737284875 2216 2 -0.24674059790744707 2216 3 -0.2357160724658911 2216 4 -0.11219689249683995 2216 5 -0.15334917484635469 2216 6 -0.1497927407311863 2216 7 -0.16310873397348757 2216 8 -0.13280853684074856 2216 9 -0.20256511770059404 2216 10-11 -0.2347104907409232 2216 12-13 -0.26966181417722623 2216 14-15 -0.3563554980927748 2216 16-17 -0.3346596102248398 2216 18-19 -0.30307816836391765 2216 20-21 -0.31314417451827126 2216 22-23 -0.3469437805800837 2216 24-25 -0.34308329569658724 2216 26-27 -0.37978193128931537 2216 28-29 -0.3319080655560285 2216 30-31 -0.3610423291692655 2216 32-33 -0.3889707567944285 2216 34-35 -0.37330052238933575 2216 36-37 -0.3643747860395692 2216 38-39 -0.3180907843544247 2216 40-41 -0.26919648359393733 2216 42-43 -0.3065857304743531 2216 44-45 -0.29306463132979843 2216 46-47 -0.23672025547883635 2216 48-49 -0.3014645018061657 2216 50-51 -0.21603746064187845 2216 52-53 -0.21811888595541973 2216 54-55 -0.1903848130714536 2216 56-57 -0.17218945826351018 2216 58-59 -0.20663280521786476 2216 60-61 -0.2589034389807452 2216 62-63 -0.23025722612356248 2216 64-65 -0.255758950112579 2216 66-67 -0.20868028833131547 2216 68-69 -0.20085175609825967 2216 70-71 -0.2572463669911471 2216 72-73 -0.21376915355146053 2216 74-75 -0.24060509090898563 2216 76-77 -0.2842071922547902 2216 78-79 -0.28525087377300906 2216 80-81 -0.24705777568488685 2216 82-83 -0.23627354630718855 2216 84-85 -0.26837960609648803 2216 86-87 -0.21619996575483214 2216 88-89 -0.22746948719322546 2216 90-91 -0.2537299158127979 2216 92-93 -0.25022036187937857 2216 94-95 -0.2567356809185597 2216 96-97 -0.2374319459538512 2216 98-99 -0.29291675836054765 2216 100 -0.26444385372595747 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15.0 3 6.0 4 8.0 5 30.0 6 76.0 7 206.0 8 492.0 9 877.0 10 1615.0 11 2646.0 12 4259.0 13 6712.0 14 8090.0 15 10916.0 16 13406.0 17 18733.0 18 22995.0 19 12971.0 20 8442.0 21 7857.0 22 8061.0 23 8432.0 24 9402.0 25 11250.0 26 14088.0 27 17744.0 28 22860.0 29 29640.0 30 37391.0 31 47612.0 32 61292.0 33 79496.0 34 111367.0 35 170546.0 36 321340.0 37 767625.0 38 1475067.0 39 816680.0 40 3393.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.94087777235612 20.01292812624664 13.469443149508312 23.576750951888936 2 15.500317517917084 20.334524781517434 40.39810094045783 23.767056760107653 3 18.06748110801375 25.78466728162238 30.3035031428785 25.844348467485368 4 12.019078739965067 16.85771778533127 39.31433540268079 31.80886807202287 5 12.200643600835878 38.23778588152074 36.08879678770511 13.472773729938275 6 30.404473500283174 38.678412008040645 17.670534174816144 13.246580316860035 7 26.893084426690745 32.91587619308893 22.729980031923763 17.46105934829657 8 25.105315192157075 36.26946444478199 20.357343670925786 18.26787669213515 9 25.166459461741358 16.16873375654909 21.38526592750271 37.279540854206836 10-11 23.71563499271102 26.783066151414324 29.082868407925417 20.41843044794924 12-13 24.671798956486818 24.22433537572583 29.378530868431096 21.725334799356254 14-15 21.710140758831734 26.060215106309094 26.853573390267947 25.376070744591228 16-17 20.834311083844305 29.63646066733468 27.376949795797312 22.152278453023705 18-19 20.720337465937945 28.075225370811918 30.19964041307983 21.00479675017031 20-21 21.73654296771996 27.39232366259455 29.961839909542153 20.909293460143342 22-23 21.37022506261147 27.165606963859222 30.079391527175588 21.38477644635372 24-25 21.224395299418234 27.34525896938661 29.99597688803217 21.434368843162986 26-27 21.025940772921253 27.298893295138754 30.144386099905972 21.53077983203402 28-29 21.043013952498118 27.387251379644884 30.17227113378866 21.397463534068336 30-31 21.241295478185254 27.762322428480836 29.777261506203768 21.219120587130146 32-33 21.031228412738162 27.46263853653638 30.179218884400555 21.3269141663249 34-35 21.18446772502034 27.32174461389998 30.109916754222905 21.383870906856778 36-37 21.449743521733616 27.665070070501272 29.585087475593664 21.300098932171448 38-39 21.233689793756998 27.85646728084412 29.649277019266094 21.26056590613279 40-41 21.131810593183673 27.564908679764077 29.74508514205874 21.558195584993502 42-43 21.213156674438796 27.48917851096359 29.647206324317736 21.650458490279874 44-45 21.335636016256824 27.697463597665976 29.462692383188426 21.504208002888767 46-47 21.499153393558526 27.880042249185422 29.130697094605573 21.490107262650476 48-49 21.464415066752515 27.867829691624653 28.905043672773107 21.76271156884972 50-51 21.47044370111723 27.915395035412725 28.71234181254349 21.901819450926556 52-53 21.560123280624715 27.875349106200943 28.690075551280223 21.87445206189412 54-55 21.557165411238387 28.278822181777358 28.549263727372832 21.614748679611417 56-57 21.42941725888915 27.964129322162652 28.637261499633976 21.96919191931422 58-59 21.335744560966127 27.54167739648435 29.072666502253774 22.049911540295746 60-61 21.5859060704958 27.928002425311966 28.644505830579753 21.841585673612478 62-63 21.33491919341484 27.85177260906955 28.767124946169098 22.046183251346513 64-65 21.369114096026586 27.64837933862835 28.891836522151866 22.090670043193203 66-67 21.267366067065414 27.76596681468809 28.701462828367085 22.265204289879417 68-69 21.7127913524001 27.815740755860613 28.600558951479115 21.87090894026017 70-71 21.614543895716075 27.697901944969537 28.58056269078231 22.10699146853208 72-73 21.43869395433272 27.87816688350552 28.60174258123232 22.08139658092944 74-75 21.552492017927065 27.913269134839574 28.398688999859207 22.135549847374154 76-77 21.56524297149747 27.840570268332964 28.348089924770775 22.246096835398788 78-79 21.586873738550338 27.991596746400326 28.403703566617 22.01782594843234 80-81 21.546823726532555 27.829202234213042 28.439054287815864 22.184919751438535 82-83 21.39161154161764 27.937791753112034 28.53932086842757 22.131275836842757 84-85 21.36898538285162 27.86706286326959 28.373045728726126 22.390906025152663 86-87 21.58907957759955 27.903712805238417 28.2901423040812 22.217065313080834 88-89 21.248234146143208 27.887385002528653 28.31959659606154 22.544784255266595 90-91 21.256454524308708 27.765029369453952 28.417192001754387 22.561324104482946 92-93 21.37510627841273 27.91549401060891 28.2458839454853 22.46351576549306 94-95 21.177544997096962 27.72084865492549 28.37007693052061 22.73152941745694 96-97 21.131321234354235 27.687065136743104 28.372468052874634 22.809145576028026 98-99 21.165649180812103 27.770838098608813 28.240666541433917 22.822846179145166 100 21.197937506864413 27.605247484177376 28.186236917698164 23.010578091260047 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 60.0 1 63.0 2 215.5 3 385.0 4 260.5 5 171.5 6 463.5 7 1141.5 8 1967.5 9 2283.5 10 1872.5 11 1491.0 12 1603.5 13 1906.0 14 2157.5 15 2410.0 16 2821.5 17 3271.5 18 3731.5 19 4307.5 20 5207.0 21 6678.5 22 8840.5 23 11994.0 24 16285.5 25 21924.0 26 29322.5 27 38458.5 28 49434.5 29 60883.5 30 72502.0 31 85056.5 32 96893.5 33 107578.5 34 117395.5 35 125331.5 36 131834.5 37 138347.5 38 144242.5 39 145789.5 40 146073.5 41 146897.5 42 145907.0 43 145793.0 44 142950.5 45 138963.5 46 137471.0 47 134516.0 48 131769.0 49 129227.5 50 125917.0 51 121991.5 52 114259.5 53 107149.0 54 102779.0 55 97298.5 56 89661.0 57 80602.5 58 72143.5 59 66114.0 60 59601.0 61 50497.5 62 42652.5 63 36356.5 64 29674.5 65 23531.0 66 18475.0 67 13825.0 68 10115.5 69 7536.0 70 5277.5 71 3540.0 72 2603.5 73 1894.0 74 1392.0 75 998.0 76 633.5 77 396.0 78 255.0 79 152.0 80 87.5 81 45.5 82 26.5 83 15.5 84 8.5 85 4.0 86 2.5 87 1.5 88 0.5 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.6287647829829316E-4 2 3.1449294785852077E-4 3 1.6934235653920347E-4 4 0.010910486114168682 5 4.8383530439772426E-4 6 0.0034110388960039563 7 9.676706087954485E-4 8 0.14009451238836104 9 0.0011853964957744244 10-11 0.0 12-13 1.3305470870937416E-4 14-15 1.8143823914914658E-4 16-17 0.0 18-19 1.4515059131931727E-4 20-21 0.0 22-23 2.5401353480880524E-4 24-25 5.322188348374967E-4 26-27 0.00584231130060252 28-29 0.007934898992122678 30-31 0.014152182653633433 32-33 0.017006810949580006 34-35 0.025546504072199838 36-37 0.03662633254290773 38-39 0.04151306911732474 40-41 0.05226630875756416 42-43 0.06900701028972542 44-45 0.10986690174611324 46-47 0.1161446648206737 48-49 0.11351985829431606 50-51 0.11503184362055895 52-53 0.10939516232432545 54-55 0.11695508895553988 56-57 0.10008133271466925 58-59 0.09172307783119857 60-61 0.09338021374876078 62-63 0.09211014607471675 64-65 3.628764782982932E-5 66-67 4.838353043977242E-5 68-69 1.3305470870937416E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 1.2095882609943107E-4 78-79 2.419176521988621E-5 80-81 3.0239706524857763E-4 82-83 1.4515059131931727E-4 84-85 0.0 86-87 6.047941304971553E-4 88-89 3.5078059568835007E-4 90-91 1.5724647392926038E-4 92-93 9.07191195745733E-4 94-95 9.19287078355676E-4 96-97 2.661094174187483E-4 98-99 3.628764782982932E-5 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4133638.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.309201060240476 #Duplication Level Percentage of deduplicated Percentage of total 1 71.58606081172695 40.30953891358134 2 16.222940675124057 18.270016565278276 3 5.522650330916168 9.329280836068861 4 2.442902293937067 5.502315057592998 5 1.2649737047611336 3.561482933865597 6 0.7494445391385071 2.5320373942709673 7 0.4671516555804408 1.8413455549792273 8 0.3157012678556469 1.4221508933325164 9 0.23783695854524978 1.2053168206452494 >10 1.104545828373651 10.763792344648488 >50 0.06093193187835177 2.329445679321412 >100 0.023999313992923457 2.1586663915777833 >500 6.455161676483458E-4 0.24564314762737213 >1k 1.29103201228384E-4 0.16242928822705463 >5k 8.606880081888502E-5 0.36653817898283336 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8298 0.2007432677946158 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6828 0.16518137292138307 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.419176521988621E-5 0.0 16-17 0.0 0.0 0.0 2.419176521988621E-5 0.0 18-19 0.0 0.0 0.0 2.419176521988621E-5 0.0 20-21 0.0 0.0 0.0 4.838353043977242E-5 0.0 22-23 0.0 0.0 0.0 9.676706087954484E-5 0.0 24-25 0.0 0.0 0.0 1.693423565392035E-4 0.0 26-27 0.0 0.0 0.0 3.5078059568835007E-4 0.0 28-29 0.0 0.0 0.0 0.0014273141479732865 0.0 30-31 0.0 0.0 0.0 0.005830215417992577 0.0 32-33 0.0 0.0 0.0 0.01557949680160672 0.0 34-35 0.0 0.0 0.0 0.030203418877027932 0.0 36-37 0.0 0.0 0.0 0.057515921810279466 0.0 38-39 0.0 0.0 0.0 0.10485920634559678 0.0 40-41 2.419176521988621E-5 0.0 0.0 0.15978660927734845 0.0 42-43 2.419176521988621E-5 0.0 0.0 0.21703642167020915 0.0 44-45 2.419176521988621E-5 0.0 0.0 0.2829347901291792 0.0 46-47 2.419176521988621E-5 0.0 0.0 0.3649085865767636 0.0 48-49 2.419176521988621E-5 0.0 0.0 0.4544906931860022 0.0 50-51 2.419176521988621E-5 0.0 0.0 0.5552010117963886 0.0 52-53 2.419176521988621E-5 0.0 0.0 0.6525365791586006 0.0 54-55 2.419176521988621E-5 0.0 0.0 0.7603229890958038 0.0 56-57 2.419176521988621E-5 0.0 0.0 0.8927970954399007 0.0 58-59 2.419176521988621E-5 0.0 0.0 1.040705547994285 0.0 60-61 2.419176521988621E-5 0.0 0.0 1.1953876948102373 0.0 62-63 2.419176521988621E-5 0.0 0.0 1.3582587541531215 0.0 64-65 2.419176521988621E-5 0.0 0.0 1.5300565748621433 0.0 66-67 2.419176521988621E-5 0.0 0.0 1.72213919070804 0.0 68-69 2.419176521988621E-5 0.0 0.0 1.9437115683569774 0.0 70-71 2.419176521988621E-5 0.0 0.0 2.183210044033851 0.0 72-73 2.419176521988621E-5 0.0 0.0 2.431115158124635 0.0 74-75 2.419176521988621E-5 0.0 0.0 2.6818628046287554 0.0 76-77 3.628764782982932E-5 0.0 0.0 2.9498470838520454 0.0 78-79 4.838353043977242E-5 0.0 0.0 3.2573969950924586 0.0 80-81 4.838353043977242E-5 0.0 0.0 3.589828136861525 0.0 82-83 4.838353043977242E-5 0.0 0.0 3.9333995865143487 0.0 84-85 4.838353043977242E-5 0.0 0.0 4.275132462010461 0.0 86-87 4.838353043977242E-5 0.0 0.0 4.632239204303811 0.0 88 4.838353043977242E-5 0.0 0.0 4.921693675159751 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6050 0.0 52.185436 1 GTATCAA 11435 0.0 43.18192 1 TCAACGC 13565 0.0 35.870342 4 ATCAACG 13600 0.0 35.84723 3 CAACGCA 13855 0.0 35.149242 5 AACGCAG 14415 0.0 33.751106 6 TATCAAC 14785 0.0 33.100628 2 ACGCAGA 16625 0.0 29.150227 7 CGCAGAG 16905 0.0 28.583908 8 GTACATG 11740 0.0 27.733234 1 TACATGG 11745 0.0 26.323072 2 GCAGAGT 18880 0.0 25.304123 9 ACATGGG 12140 0.0 24.575668 3 GTGGTAT 3125 0.0 21.6495 1 AGAGTAC 17220 0.0 21.045761 10-11 GAGTACT 12955 0.0 20.994505 12-13 CAGAGTA 18370 0.0 20.661604 10-11 CATGGGG 7640 0.0 20.141376 4 TGGTATC 3300 0.0 19.250591 2 AGTACTT 13675 0.0 19.133408 12-13 >>END_MODULE