##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032943_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1456 Sequences flagged as poor quality 0 Sequence length 100 %GC 52 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 16.51098901098901 16.0 2.0 30.0 2.0 31.0 2 16.572115384615383 16.0 2.0 30.0 2.0 31.0 3 16.64217032967033 16.0 2.0 30.0 2.0 31.0 4 18.557692307692307 20.0 2.0 35.0 2.0 37.0 5 18.421703296703296 19.0 2.0 35.0 2.0 37.0 6 18.511675824175825 21.0 2.0 35.0 2.0 37.0 7 18.511675824175825 22.0 2.0 35.0 2.0 36.0 8 18.26717032967033 21.0 2.0 35.0 2.0 37.0 9 18.89010989010989 21.0 2.0 35.0 2.0 39.0 10-11 18.744162087912088 18.5 2.0 35.0 2.0 39.0 12-13 18.597184065934066 18.0 2.0 35.0 2.0 39.0 14-15 18.852335164835164 17.0 2.0 36.0 2.0 40.0 16-17 18.595810439560438 17.0 2.0 36.0 2.0 40.0 18-19 18.518200549450547 17.5 2.0 36.0 2.0 40.0 20-21 18.243475274725274 11.5 2.0 36.0 2.0 40.0 22-23 17.886332417582416 10.0 2.0 36.0 2.0 40.0 24-25 17.588255494505496 9.5 2.0 34.5 2.0 40.0 26-27 17.37809065934066 8.0 2.0 35.0 2.0 39.5 28-29 17.040865384615387 2.0 2.0 35.0 2.0 40.0 30-31 16.713255494505496 2.0 2.0 34.5 2.0 40.0 32-33 16.305975274725277 2.0 2.0 34.0 2.0 40.0 34-35 16.10164835164835 2.0 2.0 33.5 2.0 40.0 36-37 16.0364010989011 2.0 2.0 34.0 2.0 39.5 38-39 15.948832417582418 2.0 2.0 34.0 2.0 39.5 40-41 15.811469780219781 2.0 2.0 34.0 2.0 39.0 42-43 15.690247252747252 2.0 2.0 34.0 2.0 39.0 44-45 15.470467032967033 2.0 2.0 33.0 2.0 39.0 46-47 15.424450549450551 2.0 2.0 33.5 2.0 39.0 48-49 15.089972527472527 2.0 2.0 33.0 2.0 39.0 50-51 14.845123626373628 2.0 2.0 33.0 2.0 38.5 52-53 14.774381868131869 2.0 2.0 32.5 2.0 39.0 54-55 14.651098901098901 2.0 2.0 32.0 2.0 38.5 56-57 14.404876373626374 2.0 2.0 32.0 2.0 38.0 58-59 14.243131868131869 2.0 2.0 32.0 2.0 38.0 60-61 14.008928571428571 2.0 2.0 32.0 2.0 38.0 62-63 13.711195054945055 2.0 2.0 31.0 2.0 37.5 64-65 13.446428571428571 2.0 2.0 31.0 2.0 37.0 66-67 13.084134615384617 2.0 2.0 30.5 2.0 36.0 68-69 12.900755494505495 2.0 2.0 30.5 2.0 36.0 70-71 12.805631868131869 2.0 2.0 30.0 2.0 36.0 72-73 12.687843406593407 2.0 2.0 30.0 2.0 35.5 74-75 12.547733516483516 2.0 2.0 29.0 2.0 35.0 76-77 12.405219780219781 2.0 2.0 29.0 2.0 35.0 78-79 12.197115384615385 2.0 2.0 29.5 2.0 35.0 80-81 12.172046703296704 2.0 2.0 29.0 2.0 35.0 82-83 11.945741758241759 2.0 2.0 29.0 2.0 35.0 84-85 11.875343406593405 2.0 2.0 29.0 2.0 34.0 86-87 11.797046703296704 2.0 2.0 29.0 2.0 34.0 88-89 11.58173076923077 2.0 2.0 29.0 2.0 34.0 90-91 11.385989010989011 2.0 2.0 28.5 2.0 34.0 92-93 11.22081043956044 2.0 2.0 29.0 2.0 34.0 94-95 11.153502747252748 2.0 2.0 28.0 2.0 34.0 96-97 10.955357142857142 2.0 2.0 28.0 2.0 34.0 98-99 10.748969780219781 2.0 2.0 26.5 2.0 34.0 100 10.574175824175825 2.0 2.0 27.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1102 1 -13.659562841530056 1102 2 -14.42868852459016 1102 3 -14.399453551912568 1102 4 -16.678961748633878 1102 5 -16.27131147540983 1102 6 -16.00901639344262 1102 7 -15.819398907103821 1102 8 -16.186612021857922 1102 9 -16.904918032786888 1102 10-11 -16.46338797814208 1102 12-13 -15.929644808743173 1102 14-15 -16.69398907103825 1102 16-17 -16.411612021857923 1102 18-19 -15.509972677595627 1102 20-21 -14.78866120218579 1102 22-23 -14.394398907103827 1102 24-25 -14.16707650273224 1102 26-27 -13.995218579234976 1102 28-29 -13.718715846994536 1102 30-31 -13.483469945355193 1102 32-33 -13.00928961748634 1102 34-35 -12.785245901639342 1102 36-37 -13.100683060109292 1102 38-39 -13.017486338797816 1102 40-41 -12.958060109289617 1102 42-43 -12.875546448087434 1102 44-45 -12.799999999999997 1102 46-47 -12.838387978142078 1102 48-49 -12.511202185792351 1102 50-51 -11.969808743169398 1102 52-53 -12.269398907103826 1102 54-55 -12.031284153005465 1102 56-57 -11.41516393442623 1102 58-59 -11.390573770491804 1102 60-61 -11.478961748633878 1102 62-63 -10.918169398907104 1102 64-65 -10.889344262295081 1102 66-67 -10.680874316939892 1102 68-69 -10.546038251366122 1102 70-71 -10.888524590163938 1102 72-73 -10.69398907103825 1102 74-75 -10.239890710382511 1102 76-77 -10.414480874316942 1102 78-79 -10.326092896174865 1102 80-81 -10.251775956284153 1102 82-83 -9.756284153005465 1102 84-85 -10.203825136612023 1102 86-87 -10.103688524590165 1102 88-89 -9.627732240437158 1102 90-91 -9.33155737704918 1102 92-93 -9.088934426229507 1102 94-95 -9.354508196721312 1102 96-97 -9.296584699453552 1102 98-99 -9.321038251366119 1102 100 -8.93825136612022 1103 1 0.3404371584699444 1103 2 0.07131147540983918 1103 3 0.10054644808743163 1103 4 -0.1789617486338777 1103 5 -4.771311475409831 1103 6 -0.5090163934426215 1103 7 0.680601092896179 1103 8 0.3133879781420781 1103 9 -0.4049180327868882 1103 10-11 0.5366120218579198 1103 12-13 1.5703551912568265 1103 14-15 2.3060109289617507 1103 16-17 1.5883879781420767 1103 18-19 3.740027322404373 1103 20-21 4.461338797814211 1103 22-23 4.105601092896173 1103 24-25 5.08292349726776 1103 26-27 4.754781420765024 1103 28-29 5.031284153005464 1103 30-31 6.016530054644807 1103 32-33 6.49071038251366 1103 34-35 6.4647540983606575 1103 36-37 6.399316939890708 1103 38-39 5.482513661202184 1103 40-41 4.541939890710383 1103 42-43 3.8744535519125662 1103 44-45 5.200000000000003 1103 46-47 6.411612021857922 1103 48-49 5.988797814207649 1103 50-51 7.5301912568306015 1103 52-53 6.230601092896174 1103 54-55 6.9687158469945345 1103 56-57 7.08483606557377 1103 58-59 6.6094262295081965 1103 60-61 7.521038251366122 1103 62-63 8.331830601092896 1103 64-65 8.110655737704919 1103 66-67 7.569125683060108 1103 68-69 8.203961748633878 1103 70-71 6.861475409836062 1103 72-73 5.306010928961751 1103 74-75 8.260109289617489 1103 76-77 7.085519125683058 1103 78-79 6.923907103825135 1103 80-81 7.248224043715847 1103 82-83 6.743715846994535 1103 84-85 6.296174863387977 1103 86-87 6.646311475409835 1103 88-89 6.872267759562842 1103 90-91 7.1684426229508205 1103 92-93 7.161065573770493 1103 94-95 6.645491803278688 1103 96-97 7.203415300546448 1103 98-99 7.178961748633881 1103 100 7.56174863387978 1104 1 -1.1595628415300556 1104 2 -0.4286885245901608 1104 3 -0.39945355191256837 1104 4 -0.1789617486338777 1104 5 0.22868852459016864 1104 6 0.4909836065573785 1104 7 -7.819398907103821 1104 8 -4.686612021857922 1104 9 -0.9049180327868882 1104 10-11 -2.21338797814208 1104 12-13 -0.17964480874317346 1104 14-15 -3.1939890710382493 1104 16-17 -0.9116120218579233 1104 18-19 -2.259972677595627 1104 20-21 -5.288661202185789 1104 22-23 -11.394398907103827 1104 24-25 -4.91707650273224 1104 26-27 -1.995218579234976 1104 28-29 -4.968715846994536 1104 30-31 0.01653005464480728 1104 32-33 -1.7592896174863402 1104 34-35 -2.5352459016393425 1104 36-37 -4.350683060109292 1104 38-39 -4.267486338797816 1104 40-41 1.041939890710383 1104 42-43 -0.37554644808743376 1104 44-45 -4.049999999999997 1104 46-47 -6.838387978142078 1104 48-49 -6.011202185792351 1104 50-51 0.5301912568306015 1104 52-53 -0.5193989071038256 1104 54-55 -3.2812841530054655 1104 56-57 1.3348360655737697 1104 58-59 -1.3905737704918035 1104 60-61 -7.228961748633878 1104 62-63 -6.668169398907104 1104 64-65 -4.889344262295081 1104 66-67 0.06912568306010769 1104 68-69 1.453961748633878 1104 70-71 -0.1385245901639376 1104 72-73 -5.443989071038249 1104 74-75 -2.239890710382511 1104 76-77 2.5855191256830583 1104 78-79 3.923907103825135 1104 80-81 0.2482240437158474 1104 82-83 -4.006284153005465 1104 84-85 3.046174863387977 1104 86-87 4.146311475409835 1104 88-89 0.12226775956284186 1104 90-91 3.9184426229508205 1104 92-93 5.911065573770493 1104 94-95 5.145491803278688 1104 96-97 2.703415300546448 1104 98-99 3.1789617486338813 1104 100 3.5617486338797804 1105 1 -13.659562841530056 1105 2 -14.42868852459016 1105 3 -14.399453551912568 1105 4 -16.678961748633878 1105 5 -16.27131147540983 1105 6 -16.00901639344262 1105 7 -15.819398907103821 1105 8 -16.186612021857922 1105 9 -16.904918032786888 1105 10-11 -16.46338797814208 1105 12-13 -15.929644808743173 1105 14-15 -16.69398907103825 1105 16-17 -16.411612021857923 1105 18-19 -15.509972677595627 1105 20-21 -14.78866120218579 1105 22-23 -14.394398907103827 1105 24-25 -14.16707650273224 1105 26-27 -13.995218579234976 1105 28-29 -13.718715846994536 1105 30-31 -13.483469945355193 1105 32-33 -13.00928961748634 1105 34-35 -12.785245901639342 1105 36-37 -13.100683060109292 1105 38-39 -13.017486338797816 1105 40-41 -12.958060109289617 1105 42-43 -12.875546448087434 1105 44-45 -12.799999999999997 1105 46-47 -12.838387978142078 1105 48-49 -12.511202185792351 1105 50-51 -11.969808743169398 1105 52-53 -12.269398907103826 1105 54-55 -12.031284153005465 1105 56-57 -11.41516393442623 1105 58-59 -11.390573770491804 1105 60-61 -11.478961748633878 1105 62-63 -10.918169398907104 1105 64-65 -10.889344262295081 1105 66-67 -10.680874316939892 1105 68-69 -10.546038251366122 1105 70-71 -10.888524590163938 1105 72-73 -10.69398907103825 1105 74-75 -10.239890710382511 1105 76-77 -10.414480874316942 1105 78-79 -10.326092896174865 1105 80-81 -10.251775956284153 1105 82-83 -9.756284153005465 1105 84-85 -10.203825136612023 1105 86-87 -10.103688524590165 1105 88-89 -9.627732240437158 1105 90-91 -9.33155737704918 1105 92-93 -9.088934426229507 1105 94-95 -9.354508196721312 1105 96-97 -9.296584699453552 1105 98-99 -9.321038251366119 1105 100 -8.93825136612022 1106 1 -13.659562841530056 1106 2 -14.42868852459016 1106 3 -14.399453551912568 1106 4 -16.678961748633878 1106 5 -16.27131147540983 1106 6 -16.00901639344262 1106 7 -15.819398907103821 1106 8 -16.186612021857922 1106 9 -16.904918032786888 1106 10-11 -16.46338797814208 1106 12-13 -15.929644808743173 1106 14-15 -16.69398907103825 1106 16-17 -16.411612021857923 1106 18-19 -15.509972677595627 1106 20-21 -14.78866120218579 1106 22-23 -14.394398907103827 1106 24-25 -14.16707650273224 1106 26-27 -13.995218579234976 1106 28-29 -13.718715846994536 1106 30-31 -13.483469945355193 1106 32-33 -13.00928961748634 1106 34-35 -12.785245901639342 1106 36-37 -13.100683060109292 1106 38-39 -13.017486338797816 1106 40-41 -12.958060109289617 1106 42-43 -12.875546448087434 1106 44-45 -12.799999999999997 1106 46-47 -12.838387978142078 1106 48-49 -12.511202185792351 1106 50-51 -11.969808743169398 1106 52-53 -12.269398907103826 1106 54-55 -12.031284153005465 1106 56-57 -11.41516393442623 1106 58-59 -11.390573770491804 1106 60-61 -11.478961748633878 1106 62-63 -10.918169398907104 1106 64-65 -10.889344262295081 1106 66-67 -10.680874316939892 1106 68-69 -10.546038251366122 1106 70-71 -10.888524590163938 1106 72-73 -10.69398907103825 1106 74-75 -10.239890710382511 1106 76-77 -10.414480874316942 1106 78-79 -10.326092896174865 1106 80-81 -10.251775956284153 1106 82-83 -9.756284153005465 1106 84-85 -10.203825136612023 1106 86-87 -10.103688524590165 1106 88-89 -9.627732240437158 1106 90-91 -9.33155737704918 1106 92-93 -9.088934426229507 1106 94-95 -9.354508196721312 1106 96-97 -9.296584699453552 1106 98-99 -9.321038251366119 1106 100 -8.93825136612022 1107 1 14.673770491803277 1107 2 14.904644808743171 1107 3 14.933879781420764 1107 4 11.98770491803279 1107 5 12.0620218579235 1107 6 16.324316939890714 1107 7 8.847267759562847 1107 8 10.813387978142078 1107 9 15.428415300546447 1107 10-11 9.53661202185792 1107 12-13 13.403688524590162 1107 14-15 12.472677595628415 1107 16-17 13.588387978142077 1107 18-19 16.490027322404373 1107 20-21 14.21133879781421 1107 22-23 12.605601092896173 1107 24-25 13.999590163934425 1107 26-27 5.171448087431688 1107 28-29 7.614617486338796 1107 30-31 9.683196721311472 1107 32-33 8.824043715846996 1107 34-35 10.381420765027322 1107 36-37 1.8993169398907082 1107 38-39 8.81584699453552 1107 40-41 9.375273224043715 1107 42-43 5.124453551912566 1107 44-45 8.366666666666667 1107 46-47 9.82827868852459 1107 48-49 8.488797814207649 1107 50-51 10.19685792349727 1107 52-53 10.397267759562839 1107 54-55 7.802049180327867 1107 56-57 11.251502732240438 1107 58-59 5.442759562841532 1107 60-61 10.68770491803279 1107 62-63 8.081830601092896 1107 64-65 10.110655737704919 1107 66-67 10.485792349726772 1107 68-69 6.120628415300546 1107 70-71 5.27814207650273 1107 72-73 0.6393442622950829 1107 74-75 4.760109289617489 1107 76-77 8.752185792349726 1107 78-79 9.840573770491803 1107 80-81 8.581557377049183 1107 82-83 9.243715846994535 1107 84-85 7.296174863387977 1107 86-87 9.22964480874317 1107 88-89 8.372267759562842 1107 90-91 8.835109289617488 1107 92-93 10.411065573770493 1107 94-95 8.812158469945352 1107 96-97 9.036748633879784 1107 98-99 10.512295081967213 1107 100 1.3950819672131143 1109 1 14.840437158469944 1109 2 14.07131147540984 1109 3 14.100546448087432 1109 4 11.321038251366122 1109 5 16.72868852459017 1109 6 5.9909836065573785 1109 7 17.18060109289618 1109 8 16.813387978142078 1109 9 11.095081967213112 1109 10-11 11.28661202185792 1109 12-13 11.070355191256827 1109 14-15 15.30601092896175 1109 16-17 18.088387978142077 1109 18-19 14.740027322404373 1109 20-21 13.96133879781421 1109 22-23 14.605601092896173 1109 24-25 15.83292349726776 1109 26-27 18.004781420765024 1109 28-29 16.781284153005465 1109 30-31 14.516530054644807 1109 32-33 13.74071038251366 1109 34-35 12.214754098360658 1109 36-37 13.899316939890708 1109 38-39 18.482513661202184 1109 40-41 18.54193989071038 1109 42-43 16.374453551912566 1109 44-45 11.700000000000003 1109 46-47 13.411612021857922 1109 48-49 13.238797814207649 1109 50-51 18.0301912568306 1109 52-53 20.230601092896173 1109 54-55 16.968715846994535 1109 56-57 19.584836065573768 1109 58-59 16.109426229508195 1109 60-61 12.271038251366122 1109 62-63 15.331830601092896 1109 64-65 9.360655737704919 1109 66-67 3.3191256830601077 1109 68-69 7.953961748633878 1109 70-71 15.861475409836062 1109 72-73 18.30601092896175 1109 74-75 18.01010928961749 1109 76-77 16.085519125683057 1109 78-79 15.423907103825135 1109 80-81 17.748224043715847 1109 82-83 10.493715846994535 1109 84-85 9.796174863387977 1109 86-87 14.646311475409835 1109 88-89 11.372267759562842 1109 90-91 14.91844262295082 1109 92-93 16.411065573770493 1109 94-95 19.895491803278688 1109 96-97 16.703415300546446 1109 98-99 18.17896174863388 1109 100 22.06174863387978 1110 1 9.590437158469944 1110 2 12.82131147540984 1110 3 9.600546448087432 1110 4 15.821038251366122 1110 5 16.22868852459017 1110 6 16.49098360655738 1110 7 11.680601092896179 1110 8 9.813387978142078 1110 9 13.095081967213112 1110 10-11 8.28661202185792 1110 12-13 1.5703551912568265 1110 14-15 5.556010928961751 1110 16-17 9.838387978142077 1110 18-19 11.865027322404373 1110 20-21 10.08633879781421 1110 22-23 8.730601092896173 1110 24-25 8.20792349726776 1110 26-27 10.879781420765024 1110 28-29 12.656284153005464 1110 30-31 12.016530054644807 1110 32-33 13.49071038251366 1110 34-35 13.589754098360658 1110 36-37 13.274316939890708 1110 38-39 13.232513661202184 1110 40-41 10.916939890710383 1110 42-43 13.249453551912566 1110 44-45 13.075000000000003 1110 46-47 12.411612021857922 1110 48-49 11.988797814207649 1110 50-51 12.905191256830602 1110 52-53 13.855601092896174 1110 54-55 10.593715846994535 1110 56-57 12.33483606557377 1110 58-59 11.484426229508196 1110 60-61 9.271038251366122 1110 62-63 10.581830601092896 1110 64-65 13.360655737704919 1110 66-67 14.694125683060108 1110 68-69 15.203961748633878 1110 70-71 12.611475409836062 1110 72-73 13.68101092896175 1110 74-75 12.635109289617489 1110 76-77 13.335519125683058 1110 78-79 13.048907103825135 1110 80-81 12.998224043715847 1110 82-83 9.743715846994535 1110 84-85 10.046174863387977 1110 86-87 11.396311475409835 1110 88-89 11.747267759562842 1110 90-91 10.29344262295082 1110 92-93 13.536065573770493 1110 94-95 13.020491803278688 1110 96-97 13.328415300546448 1110 98-99 11.928961748633881 1110 100 14.81174863387978 1111 1 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2210 84-85 4.046174863387977 2210 86-87 5.646311475409835 2210 88-89 5.872267759562842 2210 90-91 6.6684426229508205 2210 92-93 6.411065573770493 2210 94-95 6.770491803278688 2210 96-97 7.078415300546448 2210 98-99 7.428961748633881 2210 100 7.31174863387978 2211 1 -6.9928961748633895 2211 2 -5.762021857923495 2211 3 -6.399453551912568 2211 4 -6.678961748633878 2211 5 -9.604644808743165 2211 6 -9.342349726775955 2211 7 -9.152732240437155 2211 8 -14.186612021857922 2211 9 -14.904918032786888 2211 10-11 -11.796721311475414 2211 12-13 -12.762978142076507 2211 14-15 -9.69398907103825 2211 16-17 -9.244945355191257 2211 18-19 -15.509972677595627 2211 20-21 -14.78866120218579 2211 22-23 -14.394398907103827 2211 24-25 -14.16707650273224 2211 26-27 -13.995218579234976 2211 28-29 -13.718715846994536 2211 30-31 -13.483469945355193 2211 32-33 -13.00928961748634 2211 34-35 -12.785245901639342 2211 36-37 -13.100683060109292 2211 38-39 -13.017486338797816 2211 40-41 -12.958060109289617 2211 42-43 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66-67 -3.3058743169398923 2212 68-69 -10.546038251366122 2212 70-71 -10.888524590163938 2212 72-73 -10.69398907103825 2212 74-75 -10.239890710382511 2212 76-77 -10.414480874316942 2212 78-79 -10.326092896174865 2212 80-81 -10.251775956284153 2212 82-83 -9.756284153005465 2212 84-85 -10.203825136612023 2212 86-87 -10.103688524590165 2212 88-89 -9.627732240437158 2212 90-91 -9.33155737704918 2212 92-93 -9.088934426229507 2212 94-95 -9.354508196721312 2212 96-97 -9.296584699453552 2212 98-99 -9.321038251366119 2212 100 -8.93825136612022 2213 1 0.8404371584699444 2213 2 0.3213114754098392 2213 3 0.35054644808743163 2213 4 -2.9289617486338777 2213 5 -2.5213114754098314 2213 6 0.2409836065573785 2213 7 0.430601092896179 2213 8 -5.186612021857922 2213 9 -5.404918032786888 2213 10-11 -1.5883879781420802 2213 12-13 -4.6796448087431735 2213 14-15 -0.6939890710382493 2213 16-17 -1.0366120218579233 2213 18-19 0.49002732240437297 2213 20-21 1.2113387978142107 2213 22-23 1.6056010928961726 2213 24-25 -0.2920765027322396 2213 26-27 1.504781420765024 2213 28-29 3.4062841530054637 2213 30-31 3.6415300546448073 2213 32-33 3.74071038251366 2213 34-35 4.2147540983606575 2213 36-37 4.149316939890708 2213 38-39 3.3575136612021836 2213 40-41 3.291939890710383 2213 42-43 -1.7505464480874338 2213 44-45 2.700000000000003 2213 46-47 1.0366120218579216 2213 48-49 2.488797814207649 2213 50-51 2.4051912568306015 2213 52-53 1.9806010928961744 2213 54-55 1.0937158469945345 2213 56-57 1.8348360655737697 2213 58-59 1.1094262295081965 2213 60-61 1.8960382513661216 2213 62-63 2.7068306010928964 2213 64-65 4.610655737704919 2213 66-67 2.9441256830601077 2213 68-69 4.578961748633878 2213 70-71 1.8614754098360624 2213 72-73 1.3060109289617507 2213 74-75 3.885109289617489 2213 76-77 1.8355191256830583 2213 78-79 3.048907103825135 2213 80-81 4.498224043715847 2213 82-83 2.993715846994535 2213 84-85 5.046174863387977 2213 86-87 4.521311475409835 2213 88-89 5.122267759562842 2213 90-91 -1.0815573770491795 2213 92-93 -0.8389344262295069 2213 94-95 -1.1045081967213122 2213 96-97 -1.0465846994535521 2213 98-99 -1.0710382513661187 2213 100 -0.6882513661202196 2214 1 0.8404371584699444 2214 2 0.07131147540983918 2214 3 0.10054644808743163 2214 4 0.8210382513661223 2214 5 1.2286885245901686 2214 6 1.4909836065573785 2214 7 1.680601092896179 2214 8 1.313387978142078 2214 9 1.5950819672131118 2214 10-11 1.7866120218579198 2214 12-13 2.5703551912568265 2214 14-15 1.8060109289617507 2214 16-17 2.5883879781420767 2214 18-19 3.740027322404373 2214 20-21 3.9613387978142107 2214 22-23 4.105601092896173 2214 24-25 4.58292349726776 2214 26-27 3.254781420765024 2214 28-29 5.281284153005464 2214 30-31 5.766530054644807 2214 32-33 6.24071038251366 2214 34-35 4.7147540983606575 2214 36-37 5.399316939890708 2214 38-39 5.232513661202184 2214 40-41 6.291939890710383 2214 42-43 6.624453551912566 2214 44-45 0.20000000000000284 2214 46-47 -0.33838797814207844 2214 48-49 3.988797814207649 2214 50-51 5.0301912568306015 2214 52-53 6.730601092896174 2214 54-55 6.2187158469945345 2214 56-57 5.83483606557377 2214 58-59 5.1094262295081965 2214 60-61 4.271038251366122 2214 62-63 4.581830601092896 2214 64-65 5.360655737704919 2214 66-67 5.819125683060108 2214 68-69 4.703961748633878 2214 70-71 5.111475409836062 2214 72-73 5.306010928961751 2214 74-75 6.260109289617489 2214 76-77 5.835519125683058 2214 78-79 6.173907103825135 2214 80-81 5.248224043715847 2214 82-83 6.743715846994535 2214 84-85 3.546174863387977 2214 86-87 6.146311475409835 2214 88-89 6.372267759562842 2214 90-91 3.6684426229508205 2214 92-93 6.911065573770493 2214 94-95 7.145491803278688 2214 96-97 6.703415300546448 2214 98-99 6.928961748633881 2214 100 7.56174863387978 2215 1 14.340437158469944 2215 2 9.82131147540984 2215 3 10.350546448087432 2215 4 13.071038251366122 2215 5 13.978688524590169 2215 6 9.240983606557378 2215 7 8.180601092896179 2215 8 9.063387978142078 2215 9 7.595081967213112 2215 10-11 10.03661202185792 2215 12-13 7.8203551912568265 2215 14-15 10.05601092896175 2215 16-17 9.963387978142077 2215 18-19 10.865027322404373 2215 20-21 11.71133879781421 2215 22-23 12.480601092896173 2215 24-25 12.58292349726776 2215 26-27 10.879781420765024 2215 28-29 10.656284153005464 2215 30-31 10.391530054644807 2215 32-33 11.36571038251366 2215 34-35 14.589754098360658 2215 36-37 14.524316939890708 2215 38-39 12.607513661202184 2215 40-41 13.166939890710383 2215 42-43 14.374453551912566 2215 44-45 12.200000000000003 2215 46-47 12.286612021857922 2215 48-49 14.863797814207649 2215 50-51 14.030191256830602 2215 52-53 12.480601092896174 2215 54-55 12.843715846994535 2215 56-57 15.70983606557377 2215 58-59 14.609426229508196 2215 60-61 14.146038251366122 2215 62-63 13.081830601092896 2215 64-65 14.110655737704919 2215 66-67 12.569125683060108 2215 68-69 14.078961748633878 2215 70-71 12.361475409836062 2215 72-73 12.55601092896175 2215 74-75 13.010109289617489 2215 76-77 11.835519125683058 2215 78-79 14.048907103825135 2215 80-81 14.123224043715847 2215 82-83 14.743715846994535 2215 84-85 13.796174863387977 2215 86-87 13.271311475409835 2215 88-89 12.122267759562842 2215 90-91 10.54344262295082 2215 92-93 12.536065573770493 2215 94-95 9.645491803278688 2215 96-97 12.078415300546448 2215 98-99 11.678961748633881 2215 100 12.56174863387978 2216 1 -8.659562841530056 2216 2 -1.4286885245901608 2216 3 -1.3994535519125684 2216 4 -1.6789617486338777 2216 5 9.728688524590169 2216 6 -0.009016393442621506 2216 7 8.180601092896179 2216 8 7.813387978142078 2216 9 9.095081967213112 2216 10-11 8.03661202185792 2216 12-13 10.570355191256827 2216 14-15 7.806010928961751 2216 16-17 -2.4116120218579233 2216 18-19 -15.509972677595627 2216 20-21 -14.78866120218579 2216 22-23 -14.394398907103827 2216 24-25 -14.16707650273224 2216 26-27 -13.995218579234976 2216 28-29 -13.718715846994536 2216 30-31 -13.483469945355193 2216 32-33 -13.00928961748634 2216 34-35 -12.785245901639342 2216 36-37 -13.100683060109292 2216 38-39 -13.017486338797816 2216 40-41 -12.958060109289617 2216 42-43 -12.875546448087434 2216 44-45 -12.799999999999997 2216 46-47 -12.838387978142078 2216 48-49 -12.511202185792351 2216 50-51 -11.969808743169398 2216 52-53 -12.269398907103826 2216 54-55 -12.031284153005465 2216 56-57 -11.41516393442623 2216 58-59 -11.390573770491804 2216 60-61 -11.478961748633878 2216 62-63 -10.918169398907104 2216 64-65 -10.889344262295081 2216 66-67 -10.680874316939892 2216 68-69 -10.546038251366122 2216 70-71 -10.888524590163938 2216 72-73 -10.69398907103825 2216 74-75 -10.239890710382511 2216 76-77 -10.414480874316942 2216 78-79 -10.326092896174865 2216 80-81 -10.251775956284153 2216 82-83 -9.756284153005465 2216 84-85 -10.203825136612023 2216 86-87 -10.103688524590165 2216 88-89 -9.627732240437158 2216 90-91 -9.33155737704918 2216 92-93 -9.088934426229507 2216 94-95 -9.354508196721312 2216 96-97 -9.296584699453552 2216 98-99 -9.321038251366119 2216 100 -8.93825136612022 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 598.0 3 32.0 4 22.0 5 27.0 6 25.0 7 15.0 8 31.0 9 29.0 10 17.0 11 21.0 12 11.0 13 13.0 14 17.0 15 14.0 16 14.0 17 15.0 18 11.0 19 12.0 20 9.0 21 8.0 22 15.0 23 7.0 24 19.0 25 16.0 26 19.0 27 19.0 28 31.0 29 22.0 30 30.0 31 33.0 32 24.0 33 37.0 34 46.0 35 46.0 36 59.0 37 49.0 38 36.0 39 7.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.84022038567493 20.316804407713498 13.016528925619836 25.826446280991732 2 20.798898071625345 22.65840220385675 30.922865013774103 25.6198347107438 3 20.9366391184573 25.0 28.78787878787879 25.27548209366391 4 15.426997245179063 18.044077134986225 31.47382920110193 35.05509641873278 5 17.699724517906336 38.22314049586777 28.994490358126722 15.082644628099173 6 32.231404958677686 34.29752066115703 18.52617079889807 14.94490358126722 7 29.545454545454547 30.991735537190085 20.52341597796143 18.939393939393938 8 29.40771349862259 33.33333333333333 17.83746556473829 19.421487603305785 9 28.512396694214875 17.768595041322314 16.735537190082646 36.98347107438016 10-11 26.170798898071624 25.96418732782369 25.96418732782369 21.90082644628099 12-13 25.723140495867767 23.829201101928373 25.585399449035812 24.862258953168045 14-15 24.6900826446281 24.6900826446281 23.312672176308542 27.307162534435264 16-17 26.136363636363637 26.549586776859503 23.450413223140494 23.863636363636363 18-19 25.206611570247933 26.618457300275484 23.484848484848484 24.6900826446281 20-21 25.447658402203853 26.65289256198347 24.414600550964188 23.484848484848484 22-23 28.719008264462808 24.449035812672175 23.519283746556475 23.312672176308542 24-25 24.827823691460054 24.276859504132233 24.070247933884296 26.825068870523417 26-27 26.618457300275484 23.93250688705234 23.725895316804408 25.723140495867767 28-29 26.75619834710744 24.070247933884296 24.414600550964188 24.758953168044076 30-31 25.96418732782369 25.413223140495866 22.727272727272727 25.895316804407713 32-33 25.02581755593804 23.78657487091222 22.822719449225474 28.36488812392427 34-35 27.37603305785124 21.831955922865014 21.763085399449036 29.028925619834713 36-37 26.78141135972461 22.23752151462995 22.203098106712567 28.777969018932875 38-39 25.895316804407713 23.415977961432507 22.141873278236915 28.546831955922862 40-41 24.517906336088156 22.486225895316807 23.69146005509642 29.30440771349862 42-43 24.517906336088156 24.104683195592287 24.89669421487603 26.480716253443525 44-45 25.96418732782369 24.070247933884296 23.106060606060606 26.859504132231404 46-47 25.344352617079892 23.553719008264462 24.276859504132233 26.825068870523417 48-49 26.997245179063363 23.588154269972453 23.24380165289256 26.170798898071624 50-51 26.30853994490358 24.6900826446281 23.794765840220386 25.206611570247933 52-53 26.515151515151516 23.794765840220386 23.381542699724516 26.30853994490358 54-55 27.100550964187324 24.03581267217631 22.796143250688704 26.06749311294766 56-57 26.30853994490358 25.344352617079892 22.141873278236915 26.205234159779618 58-59 28.581267217630852 23.863636363636363 23.140495867768596 24.414600550964188 60-61 26.584022038567497 24.517906336088156 23.484848484848484 25.413223140495866 62-63 28.581267217630852 23.93250688705234 21.935261707988982 25.550964187327825 64-65 26.377410468319557 23.450413223140494 22.865013774104685 27.307162534435264 66-67 27.72038567493113 25.034435261707987 21.17768595041322 26.06749311294766 68-69 29.132231404958674 23.863636363636363 21.935261707988982 25.068870523415974 70-71 25.482093663911847 24.311294765840223 23.62258953168044 26.584022038567497 72-73 28.09917355371901 23.69146005509642 22.31404958677686 25.895316804407713 74-75 29.338842975206614 23.002754820936637 22.210743801652892 25.447658402203853 76-77 31.09504132231405 22.107438016528928 21.831955922865014 24.965564738292013 78-79 29.924242424242426 24.276859504132233 22.486225895316807 23.312672176308542 80-81 27.37603305785124 24.483471074380166 22.27961432506887 25.860881542699726 82-83 29.028925619834713 23.898071625344354 22.55509641873278 24.517906336088156 84-85 25.447658402203853 23.588154269972453 24.34573002754821 26.618457300275484 86-87 26.549586776859503 22.65840220385675 23.07162534435262 27.72038567493113 88-89 28.40909090909091 22.348484848484848 22.486225895316807 26.75619834710744 90-91 26.962809917355372 22.417355371900825 24.793388429752067 25.826446280991732 92-93 27.513774104683197 22.83057851239669 25.0 24.655647382920108 94-95 26.72176308539945 22.27961432506887 24.586776859504134 26.41184573002755 96-97 28.236914600550968 23.07162534435262 22.176308539944902 26.515151515151516 98-99 27.789256198347108 23.002754820936637 23.174931129476583 26.033057851239672 100 26.239669421487605 23.829201101928373 22.520661157024794 27.41046831955923 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4.0 1 2.5 2 0.5 3 0.0 4 0.0 5 0.5 6 0.5 7 0.0 8 0.0 9 0.5 10 0.5 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 0.5 18 0.0 19 0.0 20 1.0 21 1.0 22 1.0 23 2.5 24 3.0 25 3.5 26 4.0 27 2.5 28 3.0 29 4.5 30 7.5 31 13.0 32 17.5 33 18.5 34 14.5 35 13.5 36 18.5 37 24.0 38 30.0 39 29.0 40 31.5 41 39.0 42 35.0 43 35.5 44 38.5 45 39.0 46 38.5 47 42.5 48 43.5 49 34.5 50 32.5 51 43.0 52 48.5 53 48.5 54 44.0 55 41.0 56 42.5 57 37.5 58 38.5 59 40.0 60 41.0 61 36.5 62 30.5 63 28.5 64 27.0 65 24.5 66 24.5 67 25.0 68 21.5 69 22.0 70 18.5 71 14.0 72 15.0 73 14.5 74 12.0 75 11.5 76 10.5 77 8.5 78 6.5 79 4.0 80 4.5 81 8.5 82 8.5 83 4.5 84 3.5 85 3.0 86 2.5 87 2.0 88 2.5 89 3.0 90 1.0 91 0.5 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27472527472527475 2 0.27472527472527475 3 0.27472527472527475 4 0.27472527472527475 5 0.27472527472527475 6 0.27472527472527475 7 0.27472527472527475 8 0.27472527472527475 9 0.27472527472527475 10-11 0.27472527472527475 12-13 0.27472527472527475 14-15 0.27472527472527475 16-17 0.27472527472527475 18-19 0.27472527472527475 20-21 0.27472527472527475 22-23 0.27472527472527475 24-25 0.27472527472527475 26-27 0.27472527472527475 28-29 0.27472527472527475 30-31 0.27472527472527475 32-33 0.2403846153846154 34-35 0.27472527472527475 36-37 0.2403846153846154 38-39 0.27472527472527475 40-41 0.27472527472527475 42-43 0.27472527472527475 44-45 0.27472527472527475 46-47 0.27472527472527475 48-49 0.27472527472527475 50-51 0.27472527472527475 52-53 0.27472527472527475 54-55 0.27472527472527475 56-57 0.27472527472527475 58-59 0.27472527472527475 60-61 0.27472527472527475 62-63 0.27472527472527475 64-65 0.27472527472527475 66-67 0.27472527472527475 68-69 0.27472527472527475 70-71 0.27472527472527475 72-73 0.27472527472527475 74-75 0.27472527472527475 76-77 0.27472527472527475 78-79 0.27472527472527475 80-81 0.27472527472527475 82-83 0.27472527472527475 84-85 0.27472527472527475 86-87 0.27472527472527475 88-89 0.27472527472527475 90-91 0.27472527472527475 92-93 0.27472527472527475 94-95 0.27472527472527475 96-97 0.27472527472527475 98-99 0.27472527472527475 100 0.27472527472527475 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1456.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.79395604395604 #Duplication Level Percentage of deduplicated Percentage of total 1 99.8623537508603 99.6565934065934 2 0.06882312456985547 0.13736263736263737 3 0.06882312456985547 0.20604395604395606 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3 0.20604395604395606 No Hit GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT 2 0.13736263736263737 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.06868131868131869 0.0 36-37 0.0 0.0 0.0 0.06868131868131869 0.0 38-39 0.0 0.0 0.0 0.06868131868131869 0.0 40-41 0.0 0.0 0.0 0.10302197802197803 0.0 42-43 0.0 0.0 0.0 0.13736263736263737 0.0 44-45 0.0 0.0 0.0 0.13736263736263737 0.0 46-47 0.0 0.0 0.0 0.20604395604395603 0.0 48-49 0.0 0.0 0.0 0.20604395604395603 0.0 50-51 0.0 0.0 0.0 0.20604395604395603 0.0 52-53 0.0 0.0 0.0 0.27472527472527475 0.0 54-55 0.0 0.0 0.0 0.37774725274725274 0.0 56-57 0.0 0.0 0.0 0.4807692307692308 0.0 58-59 0.0 0.0 0.0 0.4807692307692308 0.0 60-61 0.0 0.0 0.0 0.5837912087912088 0.0 62-63 0.0 0.0 0.0 0.7554945054945055 0.0 64-65 0.0 0.0 0.0 0.8928571428571429 0.0 66-67 0.0 0.0 0.0 0.9615384615384615 0.0 68-69 0.0 0.0 0.0 1.0645604395604396 0.0 70-71 0.0 0.0 0.0 1.3392857142857144 0.0 72-73 0.0 0.0 0.0 1.6826923076923075 0.0 74-75 0.0 0.0 0.0 1.7857142857142856 0.0 76-77 0.0 0.0 0.0 1.8887362637362637 0.0 78-79 0.0 0.0 0.0 1.9917582417582418 0.0 80-81 0.0 0.0 0.0 2.232142857142857 0.0 82-83 0.0 0.0 0.0 2.541208791208791 0.0 84-85 0.0 0.0 0.0 2.8502747252747254 0.0 86-87 0.0 0.0 0.0 3.1593406593406597 0.0 88 0.0 0.0 0.0 3.64010989010989 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE