FastQCFastQC Report
Wed 25 May 2016
SRR1032942_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032942_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5374770
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT90490.16836069264359219No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG246650.030.2421461
TACATGG248050.028.915862
ACATGGG270500.025.6124463
CATGGGG182100.023.4111214
GAGTACA170800.021.0518551
AGTACAT169050.020.130022
GTATCAA197550.018.5343441
GTATTAG23450.018.4400351
ATGGGGC82200.017.8401935
TCAACGC208650.016.895374
CATGGGA64550.016.8933814
CAACGCA210850.016.785655
AGAGTAC140450.016.664810-11
AACGCAG214800.016.5423136
ATCAACG213450.016.53733
TCGTACG2901.1507691E-616.2077542
TATACTG20900.015.9672035
GTATAAT18700.015.8349151
CCTATAC18750.015.7928353
TATACCG3604.0361556E-815.667355
Produced by FastQC (version 0.11.5)