##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032942_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5374770 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.962675798220204 34.0 31.0 34.0 30.0 34.0 2 32.07962722125784 34.0 31.0 34.0 30.0 34.0 3 32.23978105109614 34.0 31.0 34.0 30.0 34.0 4 35.56642926115908 37.0 35.0 37.0 35.0 37.0 5 35.66173510680457 37.0 37.0 37.0 35.0 37.0 6 35.707493157846756 37.0 37.0 37.0 35.0 37.0 7 35.65186063775752 37.0 37.0 37.0 35.0 37.0 8 35.66879103663971 37.0 37.0 37.0 35.0 37.0 9 37.39166178273675 39.0 39.0 39.0 35.0 39.0 10-11 37.38808730420092 39.0 39.0 39.0 35.0 39.0 12-13 37.356551536158754 39.0 39.0 39.0 35.0 39.0 14-15 38.89419351897848 41.0 40.0 41.0 36.0 41.0 16-17 38.9146790467313 41.0 40.0 41.0 36.0 41.0 18-19 38.92449053633923 41.0 40.0 41.0 36.0 41.0 20-21 38.951643698241966 41.0 40.0 41.0 36.0 41.0 22-23 38.94075309269048 41.0 40.0 41.0 36.0 41.0 24-25 38.960652827934965 41.0 40.0 41.0 36.0 41.0 26-27 38.92774211733712 41.0 40.0 41.0 36.0 41.0 28-29 38.90384128065015 41.0 40.0 41.0 35.0 41.0 30-31 38.85239219538697 41.0 39.0 41.0 35.0 41.0 32-33 38.79520565531176 41.0 39.0 41.0 35.0 41.0 34-35 38.71614431873364 41.0 39.0 41.0 35.0 41.0 36-37 38.624181964995714 41.0 39.0 41.0 35.0 41.0 38-39 38.51931543117194 41.0 39.0 41.0 35.0 41.0 40-41 38.42795487062702 41.0 39.0 41.0 34.5 41.0 42-43 38.30180584471522 41.0 38.0 41.0 34.0 41.0 44-45 38.15002837330714 40.0 38.0 41.0 34.0 41.0 46-47 37.99460497844559 40.0 38.0 41.0 33.0 41.0 48-49 37.83162572537988 40.0 37.5 41.0 33.0 41.0 50-51 37.66088716354375 40.0 37.0 41.0 33.0 41.0 52-53 37.47713651002741 40.0 37.0 41.0 33.0 41.0 54-55 37.27495697490311 40.0 36.0 41.0 32.5 41.0 56-57 37.036986140802306 39.5 35.5 41.0 32.0 41.0 58-59 36.78414146093694 39.0 35.0 41.0 32.0 41.0 60-61 36.487879481354554 39.0 35.0 41.0 31.0 41.0 62-63 36.200917620660974 38.0 35.0 40.5 31.0 41.0 64-65 35.89108826982364 37.5 35.0 40.0 31.0 41.0 66-67 35.54714518016585 37.0 35.0 40.0 31.0 41.0 68-69 35.19888051023579 36.5 35.0 39.0 30.0 41.0 70-71 34.83998599009818 36.0 34.5 39.0 30.0 41.0 72-73 34.53887766360235 35.0 34.0 39.0 30.0 40.5 74-75 34.26912053911144 35.0 34.0 37.5 30.0 40.0 76-77 33.96552819934621 35.0 34.0 37.0 30.0 39.0 78-79 33.640558851820636 35.0 34.0 36.5 29.0 39.0 80-81 33.366975238010184 35.0 34.0 36.0 29.0 38.0 82-83 33.10848185131643 35.0 34.0 36.0 29.0 37.0 84-85 32.99789897986332 35.0 34.0 35.0 29.0 37.0 86-87 32.82942042171107 35.0 34.0 35.0 29.0 36.5 88-89 32.63912251128886 35.0 34.0 35.0 29.0 36.0 90-91 32.48247394772241 35.0 34.0 35.0 29.0 36.0 92-93 32.32098033962384 35.0 34.0 35.0 28.0 36.0 94-95 32.163289033763306 35.0 34.0 35.0 27.0 35.5 96-97 32.00780424092566 35.0 34.0 35.0 27.0 35.0 98-99 31.842594566837278 35.0 33.0 35.0 27.0 35.0 100 31.715918076494436 35.0 33.0 35.0 27.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3367837545035641 1101 2 0.42065744921886505 1101 3 0.4318100549987349 1101 4 0.3703085860096351 1101 5 0.31062972048465554 1101 6 0.3181957419682462 1101 7 0.33053259752328756 1101 8 0.26998569778300663 1101 9 0.40767487164661986 1101 10-11 0.41340744185739453 1101 12-13 0.25270730522256457 1101 14-15 0.3568516168167335 1101 16-17 0.38675888453532536 1101 18-19 0.38880385434779186 1101 20-21 0.3588729158634507 1101 22-23 0.4301779867264486 1101 24-25 0.4019608486706332 1101 26-27 0.4093089867058026 1101 28-29 0.4232821830853055 1101 30-31 0.41448124317621193 1101 32-33 0.40643899768869574 1101 34-35 0.4393314504086021 1101 36-37 0.4430499667541383 1101 38-39 0.45737756807351815 1101 40-41 0.44605419220079057 1101 42-43 0.4610576512884563 1101 44-45 0.4611052275069767 1101 46-47 0.43672237762287835 1101 48-49 0.3969828023066668 1101 50-51 0.46003357050814486 1101 52-53 0.42042923271738175 1101 54-55 0.4004207683569021 1101 56-57 0.3858119534557716 1101 58-59 0.3670299864215423 1101 60-61 0.41414591018855873 1101 62-63 0.3946281393832365 1101 64-65 0.405758835174197 1101 66-67 0.4285417634272619 1101 68-69 0.4557123466894808 1101 70-71 0.416908001629146 1101 72-73 0.3045276235657397 1101 74-75 0.3084531634415484 1101 76-77 0.3213726179259595 1101 78-79 0.3835343999217997 1101 80-81 0.38579585070421984 1101 82-83 0.3348348064387352 1101 84-85 0.30056810122580657 1101 86-87 0.33095938458809826 1101 88-89 0.3387657763545562 1101 90-91 0.3568058266523195 1101 92-93 0.407124158729367 1101 94-95 0.4010010228617631 1101 96-97 0.3813455069202476 1101 98-99 0.38643952545329086 1101 100 0.4419089536266334 1102 1 0.2883236960476694 1102 2 0.29302682093002375 1102 3 0.3500150956921573 1102 4 0.3011379690549987 1102 5 0.243876176813572 1102 6 0.272832674657991 1102 7 0.3025953224512037 1102 8 0.297295446615756 1102 9 0.36032355763914126 1102 10-11 0.3866680481557907 1102 12-13 0.3862519071976962 1102 14-15 0.44835023937289264 1102 16-17 0.455439820610529 1102 18-19 0.4734937970122388 1102 20-21 0.4536731146208055 1102 22-23 0.46715406689255445 1102 24-25 0.4724229749030968 1102 26-27 0.49877597214588576 1102 28-29 0.48207585443115164 1102 30-31 0.47920194764736124 1102 32-33 0.49717029646144795 1102 34-35 0.5143296929620078 1102 36-37 0.48548996041098746 1102 38-39 0.4945284312322471 1102 40-41 0.4903872563635048 1102 42-43 0.5137180311527203 1102 44-45 0.47333433430385696 1102 46-47 0.49334350839142616 1102 48-49 0.52592784678329 1102 50-51 0.47886895512544925 1102 52-53 0.4754497840238159 1102 54-55 0.48244974934849694 1102 56-57 0.5001737204959511 1102 58-59 0.44308272578312113 1102 60-61 0.49596890209065236 1102 62-63 0.5124563027462372 1102 64-65 0.4744725063969497 1102 66-67 0.49487923221756347 1102 68-69 0.4795589881008411 1102 70-71 0.4828916346323737 1102 72-73 0.4752350229881088 1102 74-75 0.4713869462196101 1102 76-77 0.442328757348065 1102 78-79 0.4276541759418464 1102 80-81 0.4374838024293055 1102 82-83 0.45040194623599206 1102 84-85 0.44006496085386004 1102 86-87 0.4373288230389534 1102 88-89 0.40736073939240214 1102 90-91 0.4502244944760605 1102 92-93 0.46852431330773214 1102 94-95 0.48588128905890215 1102 96-97 0.5043260709552939 1102 98-99 0.4642321802208613 1102 100 0.5085616568872346 1103 1 0.15405832643220663 1103 2 0.24805896931280103 1103 3 0.2630654132060215 1103 4 0.22237100600759163 1103 5 0.18814012647519718 1103 6 0.17776211181524815 1103 7 0.202601083512306 1103 8 0.2276051830490573 1103 9 0.2635085196447591 1103 10-11 0.3076206119110694 1103 12-13 0.28803313681124365 1103 14-15 0.33356065382029954 1103 16-17 0.36528356290718733 1103 18-19 0.3500074650373719 1103 20-21 0.34661820598440585 1103 22-23 0.3061315228633674 1103 24-25 0.33634789350502103 1103 26-27 0.3775173243223193 1103 28-29 0.4068389538746331 1103 30-31 0.3998426293433752 1103 32-33 0.3850797034063618 1103 34-35 0.3531774142684583 1103 36-37 0.34576010432117954 1103 38-39 0.38127796300754113 1103 40-41 0.37436023366326765 1103 42-43 0.36857249142347115 1103 44-45 0.37064698395064966 1103 46-47 0.3950230146537166 1103 48-49 0.42516031464644755 1103 50-51 0.3854820993187147 1103 52-53 0.3488960840100219 1103 54-55 0.37075347507471434 1103 56-57 0.3209472389661556 1103 58-59 0.3232988726709749 1103 60-61 0.35646844517572873 1103 62-63 0.34837181937032113 1103 64-65 0.3119776973530435 1103 66-67 0.34763313021242936 1103 68-69 0.39286029953623824 1103 70-71 0.37858259174219455 1103 72-73 0.3934204309220135 1103 74-75 0.347575243547233 1103 76-77 0.33502805267058733 1103 78-79 0.3332613963978943 1103 80-81 0.256970637758414 1103 82-83 0.331248984708985 1103 84-85 0.28975872906606526 1103 86-87 0.29774884686241876 1103 88-89 0.34682602983020416 1103 90-91 0.2605241638243925 1103 92-93 0.3062155662258874 1103 94-95 0.26690318127572965 1103 96-97 0.29463677864440996 1103 98-99 0.31483343557823673 1103 100 0.37316326012406265 1104 1 0.03180676471301069 1104 2 0.10257757144876933 1104 3 0.13796722425767172 1104 4 0.04982766863732735 1104 5 0.13310119038458623 1104 6 0.0762222392186942 1104 7 0.053132220026832044 1104 8 0.11326097442854888 1104 9 0.1788281333719226 1104 10-11 0.17439505914813225 1104 12-13 0.17737472469237758 1104 14-15 0.2507404953486656 1104 16-17 0.2308277541129229 1104 18-19 0.2283132010617308 1104 20-21 0.17534076030899115 1104 22-23 0.2382728565682939 1104 24-25 0.22818170510554125 1104 26-27 0.23780251190333246 1104 28-29 0.22822535554691115 1104 30-31 0.22361424121031348 1104 32-33 0.23822788611892065 1104 34-35 0.24075094411890063 1104 36-37 0.2484279743972806 1104 38-39 0.24030982992000105 1104 40-41 0.26375842667410865 1104 42-43 0.21044712689921852 1104 44-45 0.22007968265312172 1104 46-47 0.2528218378920144 1104 48-49 0.24649208207024742 1104 50-51 0.27812798501356895 1104 52-53 0.2654145084470727 1104 54-55 0.2630511717501989 1104 56-57 0.26188806025616884 1104 58-59 0.20679505761307837 1104 60-61 0.27916938202258734 1104 62-63 0.2405578682868068 1104 64-65 0.277046206605462 1104 66-67 0.26088916025675957 1104 68-69 0.26420056838290407 1104 70-71 0.24439420134776668 1104 72-73 0.20945088291930603 1104 74-75 0.22872457057829365 1104 76-77 0.17321774391765388 1104 78-79 0.16125495548697444 1104 80-81 0.16952613493327817 1104 82-83 0.16707754247278217 1104 84-85 0.1268398084925053 1104 86-87 0.11780644844128574 1104 88-89 0.1206673540748966 1104 90-91 0.13960735035370675 1104 92-93 0.06910594630838318 1104 94-95 0.08916131615020362 1104 96-97 0.09060339326561007 1104 98-99 0.15187665988028698 1104 100 0.1122655652667639 1105 1 -0.03337960313693955 1105 2 0.001339273320382972 1105 3 0.030299144914096132 1105 4 0.021524161930990715 1105 5 0.04947317272875296 1105 6 0.030580572116932103 1105 7 0.05344349868224185 1105 8 0.02515970474621554 1105 9 0.07899583394647891 1105 10-11 0.04881487303796206 1105 12-13 0.07811845436179254 1105 14-15 0.11785005868970444 1105 16-17 0.1019935947056041 1105 18-19 0.10818194292392747 1105 20-21 0.1200991451353488 1105 22-23 0.11511458299142419 1105 24-25 0.12894147259060418 1105 26-27 0.11545370954872425 1105 28-29 0.1656735657131918 1105 30-31 0.09598854217404096 1105 32-33 0.173640786175568 1105 34-35 0.17779905210186087 1105 36-37 0.19532465875512628 1105 38-39 0.18260399003696648 1105 40-41 0.1955257001941817 1105 42-43 0.22073249343632995 1105 44-45 0.20036402992043634 1105 46-47 0.17283732188867873 1105 48-49 0.17406301306223781 1105 50-51 0.14029911535342166 1105 52-53 0.1771005732818054 1105 54-55 0.20843804163943247 1105 56-57 0.19600602896410635 1105 58-59 0.16861381640013917 1105 60-61 0.17231197968866496 1105 62-63 0.2123976563434482 1105 64-65 0.17466823701249012 1105 66-67 0.17562586721797402 1105 68-69 0.19673676325379574 1105 70-71 0.18145193033370077 1105 72-73 0.18614267515987137 1105 74-75 0.16203009153983317 1105 76-77 0.10234231932871296 1105 78-79 0.124149588623375 1105 80-81 0.08085629854664944 1105 82-83 0.06570798215919638 1105 84-85 0.08854292973812505 1105 86-87 0.05665244446485218 1105 88-89 0.04695612831898899 1105 90-91 0.07185227589439336 1105 92-93 0.03766422189546148 1105 94-95 0.056259147095502726 1105 96-97 -4.6843908197757855E-4 1105 98-99 0.001046661603133714 1105 100 -0.017258886347871538 1106 1 -0.02176211255471827 1106 2 0.025233278928304514 1106 3 0.05882086010501553 1106 4 0.03320849756221378 1106 5 0.05389355721592182 1106 6 0.038516408666346535 1106 7 0.08078537639130445 1106 8 0.09124156350636525 1106 9 0.06821588754425534 1106 10-11 0.11065119248693378 1106 12-13 0.11339854909643776 1106 14-15 0.10209900703447516 1106 16-17 0.07310995926667374 1106 18-19 0.0759931041906583 1106 20-21 0.06973300633208623 1106 22-23 0.10399387701696128 1106 24-25 0.10448634561288372 1106 26-27 0.16622425770320604 1106 28-29 0.12704966882225222 1106 30-31 0.1346834858940653 1106 32-33 0.16304591780160393 1106 34-35 0.15711201121123963 1106 36-37 0.17994522499244425 1106 38-39 0.1576670962206279 1106 40-41 0.1774104442799711 1106 42-43 0.18659032222893757 1106 44-45 0.08422451970196931 1106 46-47 0.10164321481015293 1106 48-49 0.16700939855600438 1106 50-51 0.17147736207009245 1106 52-53 0.17751318648882375 1106 54-55 0.19407292238292229 1106 56-57 0.18592441681489902 1106 58-59 0.143190993905975 1106 60-61 0.24304055190030738 1106 62-63 0.1240631862129149 1106 64-65 0.14255204274333266 1106 66-67 0.1485540617199419 1106 68-69 0.20719313264835648 1106 70-71 0.23550725601887024 1106 72-73 0.21320531023292233 1106 74-75 0.24830091249848607 1106 76-77 0.25364139869632396 1106 78-79 0.26517819940671217 1106 80-81 0.2556999248272973 1106 82-83 0.2570063703899095 1106 84-85 0.235486073215057 1106 86-87 0.27559163590400715 1106 88-89 0.2150980351816898 1106 90-91 0.22758616639335827 1106 92-93 0.2158565790364193 1106 94-95 0.22407264410462346 1106 96-97 0.22562793355321986 1106 98-99 0.17155384314027344 1106 100 0.22252419379945465 1107 1 -0.049459777194879706 1107 2 -0.00265830411404977 1107 3 0.05121323075901074 1107 4 0.005980482402520693 1107 5 0.022547800720126077 1107 6 0.011038376296426122 1107 7 -0.0050077727695736485 1107 8 -0.04044431594276432 1107 9 -0.026996056983932704 1107 10-11 -0.02798444560178126 1107 12-13 3.70247990566952E-4 1107 14-15 0.024544434173755292 1107 16-17 0.02301296560685273 1107 18-19 -0.007029310598845484 1107 20-21 0.0048079322400766955 1107 22-23 0.03156419751605455 1107 24-25 0.027853712172039025 1107 26-27 0.07818277613414182 1107 28-29 0.06456636311843766 1107 30-31 0.05181930558028114 1107 32-33 0.03163957520573746 1107 34-35 0.0659185132820923 1107 36-37 0.09651669495809756 1107 38-39 0.01868909153255771 1107 40-41 0.027500569166825528 1107 42-43 -3.890877626133715E-4 1107 44-45 0.020723188331608355 1107 46-47 0.06392361221270448 1107 48-49 0.05805002991340302 1107 50-51 0.02509027156516197 1107 52-53 0.021860695555488974 1107 54-55 0.01624920388959339 1107 56-57 0.0676270393767453 1107 58-59 0.07171994854075336 1107 60-61 0.03656423320118307 1107 62-63 0.024224437741644067 1107 64-65 -0.022634182556558358 1107 66-67 -0.03814864469147494 1107 68-69 0.019589989062893665 1107 70-71 0.05091613447076071 1107 72-73 0.10578240263767924 1107 74-75 0.07032721430900324 1107 76-77 0.024763913178759367 1107 78-79 0.08228824396491774 1107 80-81 0.06694316431215697 1107 82-83 0.0191101400138578 1107 84-85 0.040341683107342874 1107 86-87 -0.02478729523375023 1107 88-89 0.016177856832506166 1107 90-91 0.010428354381637917 1107 92-93 0.042107467210108496 1107 94-95 0.005125926409426995 1107 96-97 -0.00496803932423262 1107 98-99 -0.056842085492178285 1107 100 -0.009354049580394985 1108 1 0.03134287924280699 1108 2 0.10965133782959668 1108 3 0.09273684196622156 1108 4 0.05021433035076939 1108 5 0.038063976070965566 1108 6 0.013168498893378455 1108 7 0.05090984933287501 1108 8 0.014408689359058258 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2214 72-73 -0.3332893493932616 2214 74-75 -0.3018456511746166 2214 76-77 -0.26154994351634997 2214 78-79 -0.28689710364713505 2214 80-81 -0.28848600867812735 2214 82-83 -0.26347947970250374 2214 84-85 -0.26293051885701857 2214 86-87 -0.2579459274826803 2214 88-89 -0.2495411992513965 2214 90-91 -0.2496613930465017 2214 92-93 -0.2470767358457877 2214 94-95 -0.2613270682214974 2214 96-97 -0.3120233226794795 2214 98-99 -0.28578173505107074 2214 100 -0.2833785741207784 2215 1 -0.22222803530724633 2215 2 -0.2623152154241204 2215 3 -0.25286942082570363 2215 4 -0.23204310902309544 2215 5 -0.2559563260291071 2215 6 -0.21074354300563414 2215 7 -0.23992305012352944 2215 8 -0.25611690176737767 2215 9 -0.3658244085003943 2215 10-11 -0.3524333894281284 2215 12-13 -0.35066509794331324 2215 14-15 -0.42038805328468953 2215 16-17 -0.39983848751447937 2215 18-19 -0.4035595486390804 2215 20-21 -0.421407372097228 2215 22-23 -0.3847277091008934 2215 24-25 -0.3608476034369019 2215 26-27 -0.3436804689427433 2215 28-29 -0.3659503777362687 2215 30-31 -0.37276524382502174 2215 32-33 -0.40720497633994057 2215 34-35 -0.4140506421628487 2215 36-37 -0.42529706707630055 2215 38-39 -0.3783041404621912 2215 40-41 -0.39218912293681285 2215 42-43 -0.39584216475683576 2215 44-45 -0.41620477514186405 2215 46-47 -0.3660781593328153 2215 48-49 -0.3623757772403735 2215 50-51 -0.34468857079590265 2215 52-53 -0.38704230697307906 2215 54-55 -0.3913722241589497 2215 56-57 -0.39332971293256946 2215 58-59 -0.3707369652992014 2215 60-61 -0.34283079496162827 2215 62-63 -0.37205810955421015 2215 64-65 -0.33326137041370885 2215 66-67 -0.3663667473067278 2215 68-69 -0.32579505344449444 2215 70-71 -0.2787795272841507 2215 72-73 -0.33611352178690623 2215 74-75 -0.2908960839004777 2215 76-77 -0.2938292993283653 2215 78-79 -0.27469973288313554 2215 80-81 -0.2974025251493799 2215 82-83 -0.3068807059363863 2215 84-85 -0.26821216401760495 2215 86-87 -0.32637763463093705 2215 88-89 -0.29227537101548506 2215 90-91 -0.28614507956178414 2215 92-93 -0.2567467580136267 2215 94-95 -0.29244374357900327 2215 96-97 -0.31093688905390593 2215 98-99 -0.35498176856901154 2215 100 -0.3164255220177701 2216 1 0.02482860886708238 2216 2 -0.06274171653306837 2216 3 -0.18835917689938242 2216 4 -0.08246327340798132 2216 5 -0.14964231158638341 2216 6 -0.08106907817005293 2216 7 -0.11164365406462906 2216 8 -0.12325314432344214 2216 9 -0.14945693496560608 2216 10-11 -0.21638578657570662 2216 12-13 -0.21650306932247787 2216 14-15 -0.2955308012210267 2216 16-17 -0.3341020733458677 2216 18-19 -0.3537282210061292 2216 20-21 -0.360436298616591 2216 22-23 -0.3285182051698641 2216 24-25 -0.3887742158121057 2216 26-27 -0.3743826896394111 2216 28-29 -0.4005732243203397 2216 30-31 -0.4316010099111267 2216 32-33 -0.477633898247106 2216 34-35 -0.4360392564145883 2216 36-37 -0.4682062354880969 2216 38-39 -0.4737547370754669 2216 40-41 -0.4296119968467451 2216 42-43 -0.38156393822536927 2216 44-45 -0.42077706585509134 2216 46-47 -0.368683662557558 2216 48-49 -0.3385715845180215 2216 50-51 -0.3159091005864241 2216 52-53 -0.33295132962809504 2216 54-55 -0.35697000179025196 2216 56-57 -0.35289290182413424 2216 58-59 -0.32963932151049136 2216 60-61 -0.3595392551304144 2216 62-63 -0.41701652061237127 2216 64-65 -0.3828035976320763 2216 66-67 -0.3969414005397027 2216 68-69 -0.3547993582166029 2216 70-71 -0.3664901223917951 2216 72-73 -0.39888272149540427 2216 74-75 -0.38882267264926185 2216 76-77 -0.42857805548880634 2216 78-79 -0.39538963472974586 2216 80-81 -0.37834793025303526 2216 82-83 -0.37280191598170376 2216 84-85 -0.4179268821961415 2216 86-87 -0.36625214225253444 2216 88-89 -0.3344158178607586 2216 90-91 -0.2684710413747311 2216 92-93 -0.3493819701351626 2216 94-95 -0.3487224203853998 2216 96-97 -0.28850406297351583 2216 98-99 -0.32817623435126464 2216 100 -0.34526004983772296 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 43698.0 3 6787.0 4 4548.0 5 4059.0 6 3822.0 7 5210.0 8 6423.0 9 6690.0 10 6460.0 11 6706.0 12 7096.0 13 7975.0 14 8789.0 15 8990.0 16 9337.0 17 8704.0 18 8346.0 19 7817.0 20 8124.0 21 8605.0 22 10302.0 23 15264.0 24 23887.0 25 30941.0 26 30399.0 27 27066.0 28 31212.0 29 40016.0 30 49205.0 31 61730.0 32 82912.0 33 114867.0 34 174083.0 35 294325.0 36 624548.0 37 1306509.0 38 1720283.0 39 558285.0 40 750.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.13844499876274 17.93068058697827 12.400309751262437 24.53056466299655 2 16.373894867298425 19.665413056439274 38.438415513137954 25.522276563124347 3 18.976321330390878 24.366417580846136 29.045006751608582 27.6122543371544 4 12.973212420356193 15.735110159736365 36.77608345185174 34.51559396805571 5 13.823904277871241 37.812642850958326 34.34530387511882 14.018148996051611 6 32.53499131466497 36.11419842336358 17.246287947950183 14.104522314021272 7 29.800673669000986 31.699161544634762 20.913959173945507 17.58620561241875 8 27.179419479375927 34.36365406948544 19.494253241681132 18.962673209457503 9 26.852798362047036 15.947492703802885 19.264753363028305 37.93495557112177 10-11 25.57171026171927 25.41033284673707 27.479602058600094 21.53835483294356 12-13 26.079392943643885 23.002282382346895 27.289150547116353 23.629174126892863 14-15 23.453640135306166 24.589387114447476 24.474031484239028 27.482941266007327 16-17 23.447088224089836 27.785054719498564 24.987860385496067 23.779996670915533 18-19 23.489401308967224 27.015642049420062 26.33813611952092 23.156820522091788 20-21 24.650768590710516 26.072941386096915 25.83251619863071 23.443773824561852 22-23 24.56997962311704 25.899345943763976 25.63771786358511 23.89295656953388 24-25 24.21041822583929 25.87397841706325 25.773769331607078 24.141834025490382 26-27 23.913240395898764 26.116480405450382 25.941618757293465 24.028660441357392 28-29 23.722050669363355 26.422050939329868 25.971439590849922 23.884458800456855 30-31 23.984452102994393 26.20036816028872 25.645595496427553 24.16958424028934 32-33 24.011692039978524 26.070990657222943 25.982475531963523 23.93484177083501 34-35 24.306161371364567 25.890921223469533 25.950919679987134 23.85199772517877 36-37 24.399095613344915 26.042774048184057 25.632508395774913 23.92562194269611 38-39 24.001903901564603 26.26650600363875 25.779387593845254 23.95220250095139 40-41 23.843646188912395 26.21075775647215 25.923396191425923 24.02219986318953 42-43 23.935806039517296 26.038712925652913 25.87801576007125 24.147465274758545 44-45 23.92928992284205 26.176885236332247 25.80607050618957 24.087754334636134 46-47 24.120003698024806 26.190273803730406 25.844007593798974 23.845714904445813 48-49 24.059964165701786 25.996783404347713 25.814083229272843 24.129169200677655 50-51 23.626466630131844 26.345554995056997 25.879056328987698 24.148922045823458 52-53 23.844773867063523 26.177236469973952 25.861196334440926 24.1167933285216 54-55 23.73444333420357 26.2580429875502 25.87208945057229 24.135424227673948 56-57 23.78903687813907 26.063881638393227 25.974510841760434 24.17257064170727 58-59 24.02334215985365 25.695071017938524 26.075873564355966 24.20571325785186 60-61 24.060284567783363 26.19819716821288 25.741982280778373 23.999535983225385 62-63 23.670296210959837 26.104043543359296 25.905708688431517 24.319951557249347 64-65 23.840302214284197 26.10168636255808 25.804786300850534 24.253225122307185 66-67 23.688979242082112 25.939856204109503 25.828572939462763 24.54259161434563 68-69 23.878688458962092 26.143827846390106 25.785708267445486 24.191775427202323 70-71 23.96479465332157 25.930734975570513 25.763679204135283 24.340791166972632 72-73 23.722074174923883 25.896892297700692 26.04409964071428 24.336933886661143 74-75 23.85725069115912 26.202201654611102 25.794088206203746 24.146459448026025 76-77 23.845808023524704 25.926618987544348 25.798674640681945 24.428898348249 78-79 23.850831026903606 26.053934193926665 25.823311392265534 24.271923386904195 80-81 23.74302825416722 26.10841309155801 26.022119291565982 24.12643936270879 82-83 23.729396768130716 25.924359452266195 26.06067472803045 24.285569051572644 84-85 23.75072756068778 26.07574712057268 25.841196078117395 24.332329240622148 86-87 23.943732517191176 26.06687144699703 25.889154965531997 24.1002410702798 88-89 23.536167251123576 26.212380357630284 25.81302629516135 24.43842609608479 90-91 23.77995603760524 25.975348141130976 25.810814987430536 24.433880833833243 92-93 23.95413143045617 25.985939743112702 25.879627168690405 24.180301657740724 94-95 23.752658750491534 25.833717499195675 26.01016692782498 24.403456822487815 96-97 23.740294129037252 25.839968378186757 26.12723460317082 24.292502889605167 98-99 23.691434255919912 26.016595219758244 25.95371126574182 24.338259258580027 100 23.812361781838074 25.687902412904172 25.7160720143853 24.783663790872453 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4228.0 1 2472.5 2 679.5 3 575.0 4 479.0 5 405.5 6 362.0 7 348.0 8 346.5 9 352.5 10 335.5 11 327.0 12 324.5 13 343.0 14 395.0 15 449.0 16 535.5 17 651.5 18 813.5 19 1035.0 20 1358.5 21 1750.5 22 2445.0 23 3806.5 24 5640.5 25 8118.5 26 11616.0 27 15928.0 28 23205.0 29 31902.5 30 38635.0 31 46508.0 32 55317.0 33 65362.0 34 79088.0 35 93210.0 36 105150.0 37 117324.5 38 128793.5 39 137379.5 40 150161.5 41 162464.5 42 165849.0 43 173725.0 44 183712.0 45 187187.5 46 193901.5 47 200578.0 48 210920.5 49 216298.0 50 208945.0 51 206504.5 52 202992.0 53 198107.0 54 194026.5 55 184960.0 56 168663.5 57 150876.0 58 140517.0 59 136764.0 60 129733.0 61 115261.5 62 102410.5 63 89069.0 64 72925.0 65 58533.0 66 47296.5 67 36433.5 68 26839.5 69 19974.0 70 13982.0 71 9949.0 72 7742.5 73 5900.5 74 4707.0 75 3510.5 76 2366.0 77 1719.0 78 1155.0 79 702.0 80 446.0 81 249.5 82 169.5 83 129.5 84 102.0 85 91.0 86 74.5 87 57.0 88 40.5 89 35.5 90 30.0 91 20.5 92 17.5 93 16.5 94 10.0 95 10.5 96 9.0 97 5.5 98 4.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07458923823717108 2 0.0771753954122688 3 0.07810566777741187 4 0.07992900161309228 5 0.07860801485458913 6 0.0785708039599834 7 0.07777076972596036 8 0.07771495338405178 9 0.07747308256911459 10-11 0.0768404973608173 12-13 0.07564974873343418 14-15 0.07418922112015956 16-17 0.07300777521642786 18-19 0.07245891452099346 20-21 0.07197517289111907 22-23 0.07401246937078237 24-25 0.0744124864877939 26-27 0.07353803046455941 28-29 0.06946343750523278 30-31 0.07463575185542823 32-33 0.07324034330771363 34-35 0.07067279157991876 36-37 0.06727729744714657 38-39 0.06872852233676977 40-41 0.07137049585377607 42-43 0.07644978296745722 44-45 0.07746377984546315 46-47 0.08066391678155531 48-49 0.08365009107366456 50-51 0.08467339067532192 52-53 0.08495247238486484 54-55 0.08491526149025912 56-57 0.08274772687947576 58-59 0.08189187630354415 60-61 0.07952898449608076 62-63 0.08013366153342376 64-65 0.07955689266703506 66-67 0.07890570201143492 68-69 0.07846847399981767 70-71 0.07644048024380579 72-73 0.07654281020397152 74-75 0.07594743589027995 76-77 0.07582650048281135 78-79 0.0761986094288686 80-81 0.07747308256911459 82-83 0.07687770825542303 84-85 0.07598464678488567 86-87 0.07697073549193734 88-89 0.0739008366869652 90-91 0.07099838690771884 92-93 0.07098908418406741 94-95 0.07118444138074745 96-97 0.0705890670670559 98-99 0.07005881181892434 100 0.06943552933427849 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5374770.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.26419336337208 #Duplication Level Percentage of deduplicated Percentage of total 1 64.91019496962555 18.346343018756787 2 12.699360826863865 7.178743800034261 3 5.550287459792138 4.706231939575927 4 3.4769706872997665 3.9309508729846927 5 2.3249939348469297 3.285703907159047 6 1.6896654614665254 2.8654218793380712 7 1.2865578405775906 2.545446370547322 8 1.0372732284503623 2.3454152879656216 9 0.8077018224848433 2.0546136441593683 >10 5.503305650651744 29.59685718467507 >50 0.4744510287188435 9.239522924558102 >100 0.23012032445782377 11.45828409139331 >500 0.007002733957411494 1.2929274303526215 >1k 0.0020479673438622103 0.9845719619890215 >5k 6.606346270508741E-5 0.16896568651077976 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9049 0.16836069264359219 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 1.860544730286133E-5 0.0 6 0.0 0.0 0.0 1.860544730286133E-5 0.0 7 0.0 0.0 0.0 1.860544730286133E-5 0.0 8 0.0 0.0 0.0 1.860544730286133E-5 0.0 9 0.0 0.0 0.0 1.860544730286133E-5 0.0 10-11 0.0 0.0 0.0 2.7908170954291997E-5 0.0 12-13 0.0 0.0 0.0 7.442178921144533E-5 0.0 14-15 0.0 0.0 0.0 1.1163268381716799E-4 0.0 16-17 0.0 0.0 0.0 1.67449025725752E-4 0.0 18-19 0.0 0.0 0.0 2.0465992033147465E-4 0.0 20-21 0.0 0.0 0.0 2.3256809128576665E-4 0.0 22-23 0.0 0.0 0.0 3.4420077510293465E-4 0.0 24-25 0.0 0.0 0.0 6.511906556001466E-4 0.0 26-27 0.0 0.0 0.0 0.0012279595219888478 0.0 28-29 0.0 0.0 0.0 0.0035443377111950836 0.0 30-31 0.0 0.0 0.0 0.011619101840636902 0.0 32-33 0.0 0.0 0.0 0.029378001291218044 0.0 34-35 0.0 0.0 0.0 0.05555586564634393 0.0 36-37 0.0 0.0 0.0 0.09957635396491385 0.0 38-39 0.0 0.0 0.0 0.17210038755146734 1.860544730286133E-5 40-41 0.0 0.0 0.0 0.25623422025500625 1.860544730286133E-5 42-43 0.0 0.0 0.0 0.3433728326979573 1.860544730286133E-5 44-45 0.0 0.0 0.0 0.446856330596472 1.860544730286133E-5 46-47 0.0 0.0 0.0 0.5775037815571643 1.860544730286133E-5 48-49 0.0 0.0 0.0 0.7157887686356812 1.860544730286133E-5 50-51 0.0 0.0 0.0 0.8663347454867836 1.860544730286133E-5 52-53 0.0 0.0 0.0 1.017438885756972 1.860544730286133E-5 54-55 0.0 0.0 0.0 1.1829715504105291 1.860544730286133E-5 56-57 0.0 0.0 0.0 1.3831289524947112 1.860544730286133E-5 58-59 0.0 0.0 0.0 1.6027941660759437 1.860544730286133E-5 60-61 0.0 0.0 0.0 1.833176117303624 1.860544730286133E-5 62-63 0.0 0.0 0.0 2.0724421696184208 1.860544730286133E-5 64-65 0.0 0.0 0.0 2.327206559536501 1.860544730286133E-5 66-67 0.0 0.0 0.0 2.6124373694130165 1.860544730286133E-5 68-69 0.0 0.0 0.0 2.9347302303168323 1.860544730286133E-5 70-71 0.0 0.0 0.0 3.274177313633886 1.860544730286133E-5 72-73 0.0 0.0 0.0 3.625178379726016 1.860544730286133E-5 74-75 0.0 0.0 0.0 3.9872403842396977 1.860544730286133E-5 76-77 0.0 0.0 0.0 4.372670830565774 1.860544730286133E-5 78-79 0.0 0.0 0.0 4.812819897409564 1.860544730286133E-5 80-81 0.0 0.0 0.0 5.2772397702599365 1.860544730286133E-5 82-83 0.0 0.0 0.0 5.750515836026471 1.860544730286133E-5 84-85 0.0 0.0 0.0 6.2273362395042025 1.860544730286133E-5 86-87 0.0 0.0 0.0 6.731618283200955 1.860544730286133E-5 88 0.0 0.0 0.0 7.137905435953538 1.860544730286133E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 24665 0.0 30.242146 1 TACATGG 24805 0.0 28.91586 2 ACATGGG 27050 0.0 25.612446 3 CATGGGG 18210 0.0 23.411121 4 GAGTACA 17080 0.0 21.051855 1 AGTACAT 16905 0.0 20.13002 2 GTATCAA 19755 0.0 18.534344 1 GTATTAG 2345 0.0 18.440035 1 ATGGGGC 8220 0.0 17.840193 5 TCAACGC 20865 0.0 16.89537 4 CATGGGA 6455 0.0 16.893381 4 CAACGCA 21085 0.0 16.78565 5 AGAGTAC 14045 0.0 16.6648 10-11 AACGCAG 21480 0.0 16.542313 6 ATCAACG 21345 0.0 16.5373 3 TCGTACG 290 1.1507691E-6 16.207754 2 TATACTG 2090 0.0 15.967203 5 GTATAAT 1870 0.0 15.834915 1 CCTATAC 1875 0.0 15.792835 3 TATACCG 360 4.0361556E-8 15.66735 5 >>END_MODULE