FastQCFastQC Report
Wed 25 May 2016
SRR1032942_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032942_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5374770
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT95320.17734712369087421No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88420.1645093650518999No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73680.1370849357274823No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG239250.029.5945591
TACATGG237500.028.5143572
GTATCAA261700.025.6733681
ACATGGG259950.025.2921143
CAACGCA293500.022.6853735
TCAACGC294050.022.6311594
CATGGGG174100.022.4636754
ATCAACG298000.022.3469733
AACGCAG302400.022.0486326
GGTATCA197950.021.6703451
TATCAAC313400.021.3684162
GAGTACA165000.020.2173821
AGAGTAC234850.020.14115510-11
GAGTACT136900.020.12380412-13
ACGCAGA337300.019.5712577
CATGGGA64950.019.5642474
GTACTTT143450.018.8774914-15
CGCAGAG349250.018.8071218
AGTACAT170050.018.6530422
AGTACTT139250.018.29995512-13
Produced by FastQC (version 0.11.5)