FastQCFastQC Report
Wed 25 May 2016
SRR1032941_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032941_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2524242
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26600.033.218041
GTATCAA38800.031.252571
TCAACGC39000.029.6461474
GTACATG81900.029.0378461
CAACGCA39900.028.8596425
ATCAACG40100.028.8329123
AACGCAG41100.028.2457376
TACATGG82550.027.3858222
ACATGGG84300.026.371293
TATCAAC47450.024.8619542
ACGCAGA47800.024.188287
CGCAGAG50900.022.4381088
GAGTACA57300.021.9005181
TACACCG4100.021.7804835
CATGGGG57800.021.5484374
ACACCGT4750.020.7789426
AGTACAT60500.018.5669382
GCAGAGT60100.018.37779
CATGGGA28150.017.8650054
TAGACTG9800.016.7857115
Produced by FastQC (version 0.11.5)