##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032941_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2524242 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.588681275408618 33.0 31.0 34.0 30.0 34.0 2 31.597039824232382 34.0 31.0 34.0 30.0 34.0 3 31.775621751004856 34.0 31.0 34.0 30.0 34.0 4 35.09521630651895 37.0 35.0 37.0 33.0 37.0 5 35.18976310512225 37.0 35.0 37.0 33.0 37.0 6 35.2454305094361 37.0 36.0 37.0 33.0 37.0 7 35.192765590620866 37.0 36.0 37.0 33.0 37.0 8 35.209749699117594 37.0 37.0 37.0 33.0 37.0 9 36.853952988659564 39.0 38.0 39.0 34.0 39.0 10-11 36.84537199682123 39.0 38.0 39.0 34.0 39.0 12-13 36.784141932508845 39.0 38.0 39.0 34.0 39.0 14-15 38.25763298447613 41.0 39.0 41.0 34.0 41.0 16-17 38.279296121370294 41.0 39.0 41.0 34.0 41.0 18-19 38.282302766533476 41.0 39.0 41.0 34.0 41.0 20-21 38.32610225168585 41.0 39.0 41.0 34.0 41.0 22-23 38.309277795076696 41.0 39.0 41.0 34.0 41.0 24-25 38.3402601652298 41.0 39.0 41.0 34.0 41.0 26-27 38.32296685500043 41.0 39.0 41.0 34.0 41.0 28-29 38.30186091507866 41.0 39.0 41.0 34.0 41.0 30-31 38.25571656758742 41.0 39.0 41.0 34.0 41.0 32-33 38.21102592382188 41.0 39.0 41.0 34.0 41.0 34-35 38.13912612182192 41.0 39.0 41.0 33.0 41.0 36-37 38.04707393348181 41.0 38.5 41.0 33.0 41.0 38-39 37.94948681624028 41.0 38.0 41.0 33.0 41.0 40-41 37.86636542772048 41.0 38.0 41.0 33.0 41.0 42-43 37.743108030054174 40.0 38.0 41.0 33.0 41.0 44-45 37.59554452386102 40.0 38.0 41.0 32.0 41.0 46-47 37.467962659681604 40.0 38.0 41.0 32.0 41.0 48-49 37.335026118731875 40.0 37.5 41.0 32.0 41.0 50-51 37.19469706153372 40.0 37.0 41.0 31.0 41.0 52-53 37.0413607332419 40.0 37.0 41.0 31.0 41.0 54-55 36.87773002746963 40.0 36.5 41.0 31.0 41.0 56-57 36.691852841367826 40.0 36.0 41.0 30.5 41.0 58-59 36.48346691798964 39.5 35.5 41.0 30.0 41.0 60-61 36.243929464766055 39.0 35.0 41.0 30.0 41.0 62-63 35.991135358654205 39.0 35.0 41.0 30.0 41.0 64-65 35.709914302986796 38.5 35.0 40.0 30.0 41.0 66-67 35.4103604171074 38.0 35.0 40.0 29.0 41.0 68-69 35.0955752261471 37.0 35.0 40.0 29.0 41.0 70-71 34.75448471263849 37.0 34.0 39.5 29.0 41.0 72-73 34.46243822898121 36.0 34.0 39.0 29.0 41.0 74-75 34.18436683170631 36.0 34.0 39.0 29.0 40.5 76-77 33.85929479027764 35.0 34.0 38.5 28.5 40.0 78-79 33.4976038351315 35.0 34.0 37.0 28.0 39.0 80-81 33.1817676355912 35.0 34.0 37.0 28.0 39.0 82-83 32.87356204357585 35.0 34.0 36.0 27.0 38.5 84-85 32.71357183661472 35.0 34.0 36.0 27.0 37.0 86-87 32.49238860616375 35.0 34.0 36.0 27.0 37.0 88-89 32.26666322008745 35.0 34.0 35.0 27.0 37.0 90-91 32.078674905179454 35.0 34.0 35.0 27.0 36.0 92-93 31.897700973203044 35.0 33.0 35.0 26.0 36.0 94-95 31.721767564282665 35.0 33.0 35.0 26.0 36.0 96-97 31.55680735048383 35.0 33.0 35.0 25.0 36.0 98-99 31.385509788681116 35.0 33.0 35.0 25.0 35.0 100 31.26243086043256 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4035788340521762 1101 2 0.4661285844840215 1101 3 0.4765087082212318 1101 4 0.4394366349463752 1101 5 0.40344278733964245 1101 6 0.4683716888610405 1101 7 0.466127722692562 1101 8 0.4474761180761533 1101 9 0.5511761209089485 1101 10-11 0.49206973923517694 1101 12-13 0.34945433466594267 1101 14-15 0.3779118931064289 1101 16-17 0.48694067048307943 1101 18-19 0.41370652957273535 1101 20-21 0.3205719641548441 1101 22-23 0.32579849796368165 1101 24-25 0.37267711433856476 1101 26-27 0.3073069195869209 1101 28-29 0.3243011902137809 1101 30-31 0.32061529808190414 1101 32-33 0.3177610945924485 1101 34-35 0.404030782830084 1101 36-37 0.4181113117374977 1101 38-39 0.4344187907996755 1101 40-41 0.40207708410267884 1101 42-43 0.4392476600540647 1101 44-45 0.41259323495662414 1101 46-47 0.35485976562954846 1101 48-49 0.30280665255737915 1101 50-51 0.371539548347144 1101 52-53 0.37644657477440546 1101 54-55 0.3384489703131379 1101 56-57 0.3536843016092135 1101 58-59 0.3030144486340234 1101 60-61 0.4090234346017354 1101 62-63 0.39944950633624643 1101 64-65 0.33386154836196624 1101 66-67 0.3217674846123444 1101 68-69 0.3684921573347921 1101 70-71 0.40089088945473605 1101 72-73 0.3534070643182545 1101 74-75 0.3239275938573414 1101 76-77 0.3137535925345958 1101 78-79 0.32486727828499085 1101 80-81 0.39166832623803316 1101 82-83 0.3376205253991813 1101 84-85 0.2510161184203099 1101 86-87 0.20172159998922723 1101 88-89 0.3307602412169004 1101 90-91 0.24236120161710062 1101 92-93 0.2912964813695389 1101 94-95 0.3352192955130029 1101 96-97 0.2895555943764272 1101 98-99 0.2862793394878089 1101 100 0.22907562904855894 1102 1 0.3051615678651274 1102 2 0.2945444683893683 1102 3 0.43317515514055316 1102 4 0.45915647834448947 1102 5 0.3865660209431283 1102 6 0.37207428988988767 1102 7 0.3435227383974109 1102 8 0.4403980701981709 1102 9 0.4530726539337877 1102 10-11 0.45633605600735194 1102 12-13 0.4300987756605892 1102 14-15 0.5271337535725351 1102 16-17 0.5669794169024414 1102 18-19 0.5119637488047459 1102 20-21 0.46538549435344834 1102 22-23 0.4827166902794673 1102 24-25 0.5200511831591825 1102 26-27 0.5608414138951119 1102 28-29 0.587923278650031 1102 30-31 0.6006169993516934 1102 32-33 0.6012592880566032 1102 34-35 0.631723329354493 1102 36-37 0.6396949387170778 1102 38-39 0.6407209745920994 1102 40-41 0.5993185176776592 1102 42-43 0.6049612895642866 1102 44-45 0.5503600631730379 1102 46-47 0.524548539588217 1102 48-49 0.46979980707308044 1102 50-51 0.5351497704266421 1102 52-53 0.5586498892732692 1102 54-55 0.5852153030347296 1102 56-57 0.562760023000763 1102 58-59 0.5373411817494471 1102 60-61 0.49082622766140815 1102 62-63 0.5194511650742939 1102 64-65 0.5164555169352525 1102 66-67 0.5868614266032921 1102 68-69 0.5565945397504706 1102 70-71 0.4628345476526192 1102 72-73 0.4864726036101459 1102 74-75 0.45749045683919576 1102 76-77 0.47258601108099185 1102 78-79 0.49163229252248897 1102 80-81 0.41213655821466944 1102 82-83 0.4639070149778419 1102 84-85 0.45800353115048864 1102 86-87 0.4175502140710634 1102 88-89 0.44590328610703267 1102 90-91 0.3929054590872738 1102 92-93 0.4273346068254291 1102 94-95 0.46740570364337586 1102 96-97 0.4233087018520152 1102 98-99 0.509405720440391 1102 100 0.517329169284654 1103 1 0.1066022870049892 1103 2 0.13733345279946718 1103 3 0.14882120399798993 1103 4 0.23186795030848373 1103 5 0.030723599090833886 1103 6 0.0748472961246307 1103 7 0.12294504401152295 1103 8 0.10940158905587793 1103 9 0.1874345535070887 1103 10-11 0.19723937178366668 1103 12-13 0.20581682794946943 1103 14-15 0.26177484431677556 1103 16-17 0.20742485427958002 1103 18-19 0.19853041296423157 1103 20-21 0.16777583751830605 1103 22-23 0.1867496142652314 1103 24-25 0.2468662205330645 1103 26-27 0.2594029715435937 1103 28-29 0.2638491285324349 1103 30-31 0.27626760178582543 1103 32-33 0.18733973593339215 1103 34-35 0.18995080897011718 1103 36-37 0.23397824843551263 1103 38-39 0.2425272402323202 1103 40-41 0.24470820423302087 1103 42-43 0.19243683950016077 1103 44-45 0.2615108780396511 1103 46-47 0.23802586623212818 1103 48-49 0.26100839544398013 1103 50-51 0.192537562368841 1103 52-53 0.1717535786868183 1103 54-55 0.1686885029997569 1103 56-57 0.21288042851303146 1103 58-59 0.22266911042092374 1103 60-61 0.17900664687506662 1103 62-63 0.19606639394233838 1103 64-65 0.1744950801984686 1103 66-67 0.13691247138486773 1103 68-69 0.16065372811608114 1103 70-71 0.21179810074346506 1103 72-73 0.23452316457420608 1103 74-75 0.1521658213794339 1103 76-77 0.13513845344470354 1103 78-79 0.22981252877993796 1103 80-81 0.10292865694597708 1103 82-83 0.11664771927791406 1103 84-85 0.11807472144324294 1103 86-87 0.1574407656027077 1103 88-89 0.13453385890225178 1103 90-91 0.17128991512797853 1103 92-93 0.1468570911267726 1103 94-95 0.10489216996621664 1103 96-97 0.15698793083390683 1103 98-99 0.21538241181970008 1103 100 0.226748283329389 1104 1 0.01752583544687525 1104 2 0.10497562137492977 1104 3 0.13927631605282542 1104 4 0.11089483661184829 1104 5 0.1327659145846951 1104 6 0.1438250900865512 1104 7 0.12720173662142287 1104 8 0.20696178993367909 1104 9 0.2604547315353045 1104 10-11 0.15992414787694997 1104 12-13 0.1877490571090732 1104 14-15 0.24074703031515554 1104 16-17 0.157403947570792 1104 18-19 0.15205064163357918 1104 20-21 0.03866625050167016 1104 22-23 0.11054085772375544 1104 24-25 0.12736824164628047 1104 26-27 0.11183526316716552 1104 28-29 0.12677079477939657 1104 30-31 0.07851110247923287 1104 32-33 0.17529719580571168 1104 34-35 0.17173978480937535 1104 36-37 0.14017676246310629 1104 38-39 0.21694203958111302 1104 40-41 0.19469313012159972 1104 42-43 0.17187358399652908 1104 44-45 0.13419418416076923 1104 46-47 0.1373169691959859 1104 48-49 0.028392865680586965 1104 50-51 0.11143766061658766 1104 52-53 0.07837676885746703 1104 54-55 0.08286297569158307 1104 56-57 0.17313037107377482 1104 58-59 0.0981470976861587 1104 60-61 0.14677878778904585 1104 62-63 0.12691953347580665 1104 64-65 0.09376645479584766 1104 66-67 0.10911722865620987 1104 68-69 0.12861424616107087 1104 70-71 0.1505220545508692 1104 72-73 0.1817811935582725 1104 74-75 0.1425330503794271 1104 76-77 0.0531824209823597 1104 78-79 0.04128442019273848 1104 80-81 0.012927423430447504 1104 82-83 0.0781086745706645 1104 84-85 0.06228837106098695 1104 86-87 0.03918111019098802 1104 88-89 0.11754291164509567 1104 90-91 0.144745961580675 1104 92-93 0.1322156559610157 1104 94-95 0.1419964745412976 1104 96-97 0.23167248781069816 1104 98-99 0.26348376344531843 1104 100 0.20846309059948354 1105 1 0.0254207248427214 1105 2 0.01916848555705286 1105 3 0.12317236031040224 1105 4 0.0766302255081257 1105 5 0.08817893110399666 1105 6 0.03912050621755725 1105 7 0.046199325733894625 1105 8 0.03386074748910062 1105 9 0.07174282293306078 1105 10-11 0.14913653445100294 1105 12-13 0.13074412734989949 1105 14-15 0.16918640973611332 1105 16-17 0.1067720131432992 1105 18-19 0.19502559272814324 1105 20-21 0.20658371426162603 1105 22-23 0.16876896337542036 1105 24-25 0.2294451740789114 1105 26-27 0.21090307605682312 1105 28-29 0.22740187670068934 1105 30-31 0.20220395472627217 1105 32-33 0.2220509090385363 1105 34-35 0.22274739030132906 1105 36-37 0.21874418831495746 1105 38-39 0.236806570662921 1105 40-41 0.25178228749310705 1105 42-43 0.21960256431955116 1105 44-45 0.23072937163824747 1105 46-47 0.23000064733966497 1105 48-49 0.22643846680544044 1105 50-51 0.23862306025444013 1105 52-53 0.2978869983635022 1105 54-55 0.21844931240424614 1105 56-57 0.3237874260172333 1105 58-59 0.3212456962796395 1105 60-61 0.3204365736144723 1105 62-63 0.30363645254113436 1105 64-65 0.33440246988634215 1105 66-67 0.3572996286089207 1105 68-69 0.3689658651840091 1105 70-71 0.3216997293458945 1105 72-73 0.32536358373558016 1105 74-75 0.34490199460164916 1105 76-77 0.34948027073439647 1105 78-79 0.29729180403308675 1105 80-81 0.3272832867975808 1105 82-83 0.27801649023381714 1105 84-85 0.2749959421116941 1105 86-87 0.2636533804377237 1105 88-89 0.23507021173522702 1105 90-91 0.24271586015517244 1105 92-93 0.19610224308317115 1105 94-95 0.21577702125920695 1105 96-97 0.23164077458014276 1105 98-99 0.2173782959252364 1105 100 0.21270175141454928 1106 1 0.0013874925235803914 1106 2 0.0012949845985268382 1106 3 0.04253451746362913 1106 4 0.09631623210214713 1106 5 0.07526386651488082 1106 6 0.061124888000385624 1106 7 0.04409758584174028 1106 8 0.10772220340897576 1106 9 0.09555622560130672 1106 10-11 0.16149737857944046 1106 12-13 0.15765953838125313 1106 14-15 0.1875318402341506 1106 16-17 0.1936797925719702 1106 18-19 0.16136483082528486 1106 20-21 0.2164701680362029 1106 22-23 0.2293401632808667 1106 24-25 0.1764159171336246 1106 26-27 0.14069675997635045 1106 28-29 0.19101872639659945 1106 30-31 0.14811431737113878 1106 32-33 0.1609325670710149 1106 34-35 0.1917217528497943 1106 36-37 0.20965679499368406 1106 38-39 0.27256083247597473 1106 40-41 0.2880486623700307 1106 42-43 0.21965715873747627 1106 44-45 0.25097616889882346 1106 46-47 0.23761976299430643 1106 48-49 0.2600869231845735 1106 50-51 0.22548402787370492 1106 52-53 0.2647610366995252 1106 54-55 0.25619427400012285 1106 56-57 0.22229295464583032 1106 58-59 0.16736552144391936 1106 60-61 0.20489384048723025 1106 62-63 0.11732465249493629 1106 64-65 0.1584715588318346 1106 66-67 0.10575541243013475 1106 68-69 0.1372014171560778 1106 70-71 0.18791846768922937 1106 72-73 0.1584987582075783 1106 74-75 0.1364087439091648 1106 76-77 0.11093079650331816 1106 78-79 0.16696091684911352 1106 80-81 0.1261094269533487 1106 82-83 0.10549940007563663 1106 84-85 0.1374273379094646 1106 86-87 0.1578616314256891 1106 88-89 0.06500729312055142 1106 90-91 0.04492180859451622 1106 92-93 0.02374298306404299 1106 94-95 0.028722969445912128 1106 96-97 0.021775185746676584 1106 98-99 0.012410269233210869 1106 100 0.1230770285066285 1107 1 0.1250649343379635 1107 2 0.140931925843514 1107 3 0.12667140101213192 1107 4 0.12204004522442347 1107 5 0.12598678051629264 1107 6 0.13618717728846264 1107 7 0.07037732643391337 1107 8 0.06997592934499153 1107 9 0.0920906180504133 1107 10-11 0.08404072002360863 1107 12-13 0.10180552561526923 1107 14-15 0.1110340472113549 1107 16-17 0.142553489211231 1107 18-19 0.10048521845676817 1107 20-21 0.08183629629957778 1107 22-23 0.08015726626762643 1107 24-25 0.13709135616497292 1107 26-27 0.1874623618756246 1107 28-29 0.20076099600537844 1107 30-31 0.21821929164101306 1107 32-33 0.07716161529846488 1107 34-35 0.1207751806248396 1107 36-37 0.12233275364051366 1107 38-39 0.04055432381296242 1107 40-41 -0.0148355090619674 1107 42-43 0.03122628224668489 1107 44-45 0.0704312766621058 1107 46-47 0.0669321167483119 1107 48-49 0.11679663517567462 1107 50-51 0.028257874745996503 1107 52-53 -0.037907415155984836 1107 54-55 -0.03382146443360057 1107 56-57 0.009890231538534522 1107 58-59 0.07739400309331046 1107 60-61 0.09316599263820535 1107 62-63 0.06949843513041998 1107 64-65 0.05122897019136019 1107 66-67 -0.0011936601145521308 1107 68-69 -0.01865924007835673 1107 70-71 0.0011102092745289838 1107 72-73 0.13373589352381288 1107 74-75 -0.029549248886475254 1107 76-77 -0.013984620177055263 1107 78-79 -0.0501682312050562 1107 80-81 -0.017886861864539583 1107 82-83 -0.16111142389538458 1107 84-85 -0.1388195622064714 1107 86-87 -0.1940218844465349 1107 88-89 -0.133152209948193 1107 90-91 -0.058332814518315956 1107 92-93 -0.07336814508061096 1107 94-95 -0.08871617048631819 1107 96-97 -0.1364610598911966 1107 98-99 -0.1307976398643511 1107 100 -0.04753667459518951 1108 1 0.044223830212914095 1108 2 0.05388768744095174 1108 3 -0.005196555476619125 1108 4 0.023393698755008074 1108 5 0.05672145124439254 1108 6 0.027568855077255705 1108 7 0.06768063245584699 1108 8 0.06512888907518999 1108 9 0.09253276334817429 1108 10-11 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2214 62-63 -0.04277022716127732 2214 64-65 -0.07000912590498842 2214 66-67 0.05039820271402817 2214 68-69 -0.01919440515346338 2214 70-71 0.045429075068554425 2214 72-73 -0.029607666610310446 2214 74-75 -0.04754339411223185 2214 76-77 -0.012877830948987423 2214 78-79 -0.016752337791849925 2214 80-81 -0.014582191020807045 2214 82-83 -0.03515005131652771 2214 84-85 -0.037602418005398874 2214 86-87 -0.09033344499578533 2214 88-89 -0.12062242055502992 2214 90-91 -0.09259377516848843 2214 92-93 -0.009722227870046396 2214 94-95 1.6637880308678632E-4 2214 96-97 -0.10880072373378624 2214 98-99 -0.14515597864740215 2214 100 -0.11228410948702461 2215 1 -0.22962921370373834 2215 2 -0.2439101448822072 2215 3 -0.3259739421193366 2215 4 -0.28960007688004197 2215 5 -0.2797160195310582 2215 6 -0.31276180762608874 2215 7 -0.3230648228602391 2215 8 -0.360009502925287 2215 9 -0.413835761407789 2215 10-11 -0.34363229122510575 2215 12-13 -0.3735196605654423 2215 14-15 -0.4054408252786459 2215 16-17 -0.4351688039416217 2215 18-19 -0.4162784409941622 2215 20-21 -0.4341147100375409 2215 22-23 -0.3673413503983127 2215 24-25 -0.40299511736396454 2215 26-27 -0.43518099435560487 2215 28-29 -0.44976444691570094 2215 30-31 -0.4189026738772199 2215 32-33 -0.45942581144284844 2215 34-35 -0.4306306503845576 2215 36-37 -0.43713208606592246 2215 38-39 -0.4563854534646481 2215 40-41 -0.3756436978251969 2215 42-43 -0.38426178609854844 2215 44-45 -0.3507725332923499 2215 46-47 -0.3927488866759532 2215 48-49 -0.32390949963587445 2215 50-51 -0.37808821225817724 2215 52-53 -0.3914969064009597 2215 54-55 -0.3706926462635778 2215 56-57 -0.384933126481414 2215 58-59 -0.4064959925968523 2215 60-61 -0.4281811797224293 2215 62-63 -0.4268812017097119 2215 64-65 -0.3899654457860535 2215 66-67 -0.42034853304340203 2215 68-69 -0.4018324926673742 2215 70-71 -0.2593149138526769 2215 72-73 -0.4203792503035828 2215 74-75 -0.41475277002525246 2215 76-77 -0.3680403637863989 2215 78-79 -0.31988657904839357 2215 80-81 -0.33396851306768127 2215 82-83 -0.34741939504161934 2215 84-85 -0.269750601603441 2215 86-87 -0.3225719751552276 2215 88-89 -0.2739913849348241 2215 90-91 -0.24531753227422826 2215 92-93 -0.34240894213376905 2215 94-95 -0.3320646930660125 2215 96-97 -0.2614471662370619 2215 98-99 -0.2991796235329396 2215 100 -0.29476772317705624 2216 1 -0.06650484795619249 2216 2 -0.0595989950825313 2216 3 -0.14050053192485734 2216 4 -0.17425160690279284 2216 5 -0.14742934343482972 2216 6 -0.10861563515751982 2216 7 -0.12886520898526754 2216 8 -0.20824044158653976 2216 9 -0.24623982913415432 2216 10-11 -0.260159534131418 2216 12-13 -0.29917562908340045 2216 14-15 -0.3489670561562548 2216 16-17 -0.33457652662725934 2216 18-19 -0.27824921889640564 2216 20-21 -0.31332119719707663 2216 22-23 -0.2602401743543439 2216 24-25 -0.35369870396041847 2216 26-27 -0.3649081120071713 2216 28-29 -0.2569460810922948 2216 30-31 -0.29439684731156035 2216 32-33 -0.41877532860154076 2216 34-35 -0.3849199083566006 2216 36-37 -0.4450394160585134 2216 38-39 -0.33295335790842273 2216 40-41 -0.35269180711689785 2216 42-43 -0.2958391077747393 2216 44-45 -0.27881139463979565 2216 46-47 -0.20473324000902693 2216 48-49 -0.23405483306418517 2216 50-51 -0.21293472432066096 2216 52-53 -0.1878017527603859 2216 54-55 -0.22135599223031477 2216 56-57 -0.23715554245481485 2216 58-59 -0.14738986186753777 2216 60-61 -0.13700782308961834 2216 62-63 -0.11415438563158631 2216 64-65 -0.1592321505040104 2216 66-67 -0.19004595126011026 2216 68-69 -0.1552420997245676 2216 70-71 -0.19452675995401592 2216 72-73 -0.2224726418671068 2216 74-75 -0.23414058978793406 2216 76-77 -0.24005328334121145 2216 78-79 -0.18249521718522743 2216 80-81 -0.21817438967973146 2216 82-83 -0.2707755653177557 2216 84-85 -0.3547269706405487 2216 86-87 -0.307471609474284 2216 88-89 -0.26990478709967647 2216 90-91 -0.28991233688515194 2216 92-93 -0.2648903412495862 2216 94-95 -0.29660185181853294 2216 96-97 -0.2820653085630802 2216 98-99 -0.3153516619382266 2216 100 -0.3373528089821036 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 37934.0 3 5567.0 4 3674.0 5 3162.0 6 2415.0 7 2714.0 8 3216.0 9 3425.0 10 3591.0 11 3746.0 12 4133.0 13 4583.0 14 5126.0 15 5275.0 16 5305.0 17 5218.0 18 5003.0 19 4934.0 20 4858.0 21 5160.0 22 6056.0 23 8280.0 24 12569.0 25 16895.0 26 17664.0 27 15723.0 28 16944.0 29 21724.0 30 26073.0 31 31515.0 32 41032.0 33 55542.0 34 80545.0 35 128213.0 36 245020.0 37 513066.0 38 819939.0 39 347905.0 40 498.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.80344387916926 19.23797274837924 13.0470464723868 23.911536900064704 2 15.452263547923986 19.723433349998594 40.454791090205305 24.369512011872114 3 18.35362386874285 25.15698633808502 29.656997043050616 26.83239275012152 4 12.254107817878612 16.27283475712311 38.46099862735722 33.01205879764105 5 12.95207879526537 38.25699334940936 35.37591656748006 13.415011287845207 6 31.42518547417635 37.69217471841864 17.571001957034063 13.311637850370946 7 28.095159932059204 32.369688953594405 22.06620738435871 17.468943729987675 8 25.64554446652383 36.790364965355806 19.782516299185 17.781574268935362 9 25.470330268965757 17.040444973608533 20.476390413671783 37.01283434375392 10-11 24.29729165555709 26.97092466230689 28.441293961459007 20.290489720677012 12-13 24.70126464090205 24.271708974995615 28.61165047581383 22.415375908288507 14-15 22.001930392098753 26.261142919784458 25.395677848522695 26.34124883959409 16-17 22.029544287688097 29.661977191587884 26.029778199788684 22.278700320935332 18-19 22.004083923214633 28.683881308924946 27.806973672551504 21.505061095308918 20-21 22.864541302744332 27.985768795658167 27.400539458428458 21.749150443169043 22-23 22.710012111985314 27.686472042326532 27.365514343088336 22.238001502599815 24-25 22.435719407211288 27.808434546262976 27.39410727946108 22.361738767064658 26-27 22.18216389878343 27.829799414778883 27.48630265022582 22.50173403621187 28-29 22.313550097951822 27.8819273683716 27.550637514428146 22.25388501924843 30-31 22.40571676194471 28.14053785741161 27.216825181473652 22.236920199170033 32-33 22.135207853574286 27.987704952966848 27.635647627884925 22.24143956557395 34-35 22.393028650310956 27.759604912977064 27.585939634020395 22.261426802691584 36-37 22.566698916757698 27.9811859272258 27.185161707347966 22.266953448668538 38-39 22.371670055920205 28.1616542120932 27.347460299746256 22.119215432240335 40-41 22.245375160861382 27.86866495556935 27.589164583298643 22.29679530027062 42-43 22.173988718672945 27.847163273955772 27.53783272758414 22.44101527978714 44-45 22.189468701103063 27.95741663397372 27.619280030909454 22.233834634013764 46-47 22.319206271387003 27.999622539992618 27.45773746427363 22.223433724346744 48-49 22.208597908798954 27.917658834988373 27.544925374998485 22.32881788121418 50-51 22.210459159903237 27.870924049343003 27.54257600755585 22.376040783197908 52-53 22.26311538493564 27.814362223282217 27.536093106356457 22.386429285425685 54-55 22.25749821323603 28.083587841876597 27.401078699890068 22.25783524499731 56-57 22.172926188049512 27.878589147898474 27.505402815828067 22.443081848223944 58-59 22.13747782596525 27.53561128173653 27.760741669422313 22.566169222875914 60-61 22.34387981848037 27.954902788353515 27.37196794086319 22.329249452302925 62-63 22.125500643206212 27.99595421410589 27.450601782897436 22.42794335979046 64-65 22.17650943233517 27.769855740056542 27.536859943020804 22.516774884587484 66-67 22.030127327269224 27.861949391038323 27.451963831030678 22.655959450661772 68-69 22.317820928365396 27.891660967220748 27.45081130373586 22.339706800677988 70-71 22.280272727020424 27.72837042155966 27.504084680653502 22.487272170766413 72-73 22.102433997864587 27.819956078532126 27.607425812644294 22.470184110958996 74-75 22.205676099740607 27.942356851095802 27.4645082699225 22.387458779241094 76-77 22.259550071553306 27.80595703312782 27.38292128977929 22.55157160553959 78-79 22.259893792734864 27.888843162302685 27.43785479403609 22.41340825092636 80-81 22.196462098686407 27.775040971151594 27.536182180527607 22.492314749634396 82-83 22.141889313449997 27.793970571472464 27.612760621874642 22.451379493202893 84-85 22.15811909899066 27.80607936285156 27.476471292558436 22.559330245599345 86-87 22.276358812332465 27.83178000658943 27.479768822795464 22.41209235828264 88-89 22.07046442643086 27.76274296335003 27.520899625499645 22.645892984719467 90-91 22.12531384161965 27.729029551957268 27.540040699801892 22.60561590662119 92-93 22.2931167328511 27.821249891718917 27.439307587842464 22.446325787587508 94-95 22.10751292486639 27.649748757763792 27.511941662720307 22.730796654649513 96-97 22.189409400932767 27.660752429840297 27.568815741273745 22.581022427953194 98-99 22.179152536594145 27.75959622549653 27.432248994805736 22.629002243103585 100 22.278490848485426 27.545924079377613 27.379932953381353 22.795652118755612 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2431.0 1 1544.5 2 614.5 3 516.5 4 414.0 5 334.0 6 288.5 7 268.0 8 286.5 9 308.0 10 287.0 11 270.0 12 275.0 13 302.0 14 330.0 15 365.5 16 456.5 17 571.0 18 738.0 19 1054.0 20 1434.0 21 1956.0 22 2964.5 23 4625.0 24 6836.0 25 9582.5 26 13250.5 27 17940.0 28 23575.5 29 30224.0 30 36951.5 31 43256.5 32 49467.5 33 56403.5 34 62638.5 35 67674.5 36 73362.5 37 78947.0 38 83436.5 39 85342.0 40 87381.0 41 90352.5 42 90904.0 43 90707.5 44 90022.5 45 89358.5 46 89602.5 47 88480.5 48 86405.5 49 83946.0 50 81346.5 51 78685.5 52 74270.5 53 70380.5 54 68030.0 55 64399.0 56 59122.5 57 53942.0 58 49161.0 59 45252.0 60 40780.0 61 35387.5 62 30701.5 63 26012.5 64 21560.0 65 17749.0 66 14353.0 67 11086.0 68 8460.5 69 6503.5 70 4885.0 71 3736.5 72 2924.5 73 2204.5 74 1663.5 75 1216.5 76 823.0 77 589.0 78 422.5 79 282.0 80 186.5 81 135.5 82 111.0 83 80.0 84 66.5 85 59.5 86 47.0 87 38.5 88 25.0 89 19.0 90 17.5 91 13.0 92 12.5 93 11.0 94 7.0 95 3.5 96 3.0 97 3.0 98 1.5 99 2.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07804323040342408 2 0.08038056573022713 3 0.08113326693716372 4 0.08319329129299013 5 0.08160865717312366 6 0.08152942546713032 7 0.08105403523117038 8 0.0804201815832238 9 0.08022210231824049 10-11 0.07996459927376218 12-13 0.07861766027187567 14-15 0.07750841638796914 16-17 0.07633974872456761 18-19 0.07576531885611601 20-21 0.07517108106116609 22-23 0.07713206578450085 24-25 0.07766687979995579 26-27 0.07715187371099919 28-29 0.07243758720439641 30-31 0.07824130966840738 32-33 0.07628032494507261 34-35 0.07372510242678793 36-37 0.0700606360245967 38-39 0.07164527014446316 40-41 0.07463626704571115 42-43 0.08006363890625383 44-45 0.0806776846277021 46-47 0.08400541627942171 48-49 0.08709545281316133 50-51 0.08806604121157956 52-53 0.08806604121157956 54-55 0.08812546499107454 56-57 0.08626351990023144 58-59 0.0850948522368299 60-61 0.08281694068952185 62-63 0.08364887360245174 64-65 0.08293578824851183 66-67 0.0824009742330569 68-69 0.08188596814410029 70-71 0.07974671208228054 72-73 0.07974671208228054 74-75 0.0792118980668256 76-77 0.07909305050783562 78-79 0.07939016940531059 80-81 0.08083614803968875 82-83 0.08040037365672546 84-85 0.07939016940531059 86-87 0.08006363890625383 88-89 0.0771716816374975 90-91 0.07412126095675454 92-93 0.07414106888325288 94-95 0.07429953229523951 96-97 0.07344779145581129 98-99 0.07305163292584467 100 0.07202162074793146 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2524242.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.67595973130621 #Duplication Level Percentage of deduplicated Percentage of total 1 78.37203286045721 49.9041440848308 2 12.895692963933273 16.422912517574076 3 3.89310805401089 7.43692175030464 4 1.6185528806244056 4.122516321985173 5 0.9054341356552311 2.8827193780666267 6 0.515119431566174 1.9680434508732625 7 0.37033182912728835 1.650686424711113 8 0.24794480289425574 1.2630498627745026 9 0.19550096929555957 1.1203840663465847 >10 0.9344326028907545 10.366458722581404 >50 0.04079022974055069 1.729588222016102 >100 0.010934836723609745 1.028566092516364 >500 6.220154038260121E-5 0.03742901927693207 >1k 6.220154038260121E-5 0.06658008614235217 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.961585299666197E-5 0.0 4 0.0 0.0 0.0 3.961585299666197E-5 0.0 5 0.0 3.961585299666197E-5 0.0 3.961585299666197E-5 0.0 6 3.961585299666197E-5 3.961585299666197E-5 0.0 3.961585299666197E-5 0.0 7 3.961585299666197E-5 3.961585299666197E-5 0.0 3.961585299666197E-5 0.0 8 3.961585299666197E-5 3.961585299666197E-5 0.0 3.961585299666197E-5 0.0 9 3.961585299666197E-5 3.961585299666197E-5 0.0 1.5846341198664789E-4 0.0 10-11 3.961585299666197E-5 3.961585299666197E-5 0.0 1.5846341198664789E-4 0.0 12-13 3.961585299666197E-5 3.961585299666197E-5 0.0 1.5846341198664789E-4 0.0 14-15 3.961585299666197E-5 7.923170599332394E-5 0.0 1.7827133848497884E-4 0.0 16-17 3.961585299666197E-5 1.188475589899859E-4 0.0 2.575030444783028E-4 0.0 18-19 3.961585299666197E-5 1.188475589899859E-4 0.0 2.773109709766338E-4 0.0 20-21 3.961585299666197E-5 1.188475589899859E-4 0.0 2.773109709766338E-4 0.0 22-23 3.961585299666197E-5 1.188475589899859E-4 0.0 3.961585299666197E-4 0.0 24-25 3.961585299666197E-5 1.386554854883169E-4 0.0 4.753902359599436E-4 0.0 26-27 3.961585299666197E-5 1.5846341198664789E-4 0.0 6.536615744449224E-4 0.0 28-29 3.961585299666197E-5 1.5846341198664789E-4 0.0 0.001564826193368148 0.0 30-31 3.961585299666197E-5 1.5846341198664789E-4 0.0 0.004258704197141161 0.0 32-33 3.961585299666197E-5 1.5846341198664789E-4 0.0 0.012122451016978564 0.0 34-35 3.961585299666197E-5 1.5846341198664789E-4 0.0 0.02214526182513404 0.0 36-37 3.961585299666197E-5 1.7827133848497884E-4 0.0 0.04254742611841496 0.0 38-39 3.961585299666197E-5 1.9807926498330983E-4 0.0 0.08129173034915035 0.0 40-41 3.961585299666197E-5 2.773109709766338E-4 0.0 0.1272857356782749 0.0 42-43 3.961585299666197E-5 2.773109709766338E-4 0.0 0.17201203371150625 0.0 44-45 3.961585299666197E-5 3.1692682397329577E-4 0.0 0.22398803284312677 0.0 46-47 3.961585299666197E-5 3.1692682397329577E-4 0.0 0.28923534272862905 0.0 48-49 3.961585299666197E-5 3.1692682397329577E-4 0.0 0.36559489937969497 0.0 50-51 3.961585299666197E-5 3.1692682397329577E-4 0.0 0.45001628211558165 0.0 52-53 3.961585299666197E-5 3.1692682397329577E-4 0.0 0.5378446282091812 0.0 54-55 3.961585299666197E-5 3.1692682397329577E-4 0.0 0.6317738156642667 0.0 56-57 3.961585299666197E-5 3.1692682397329577E-4 0.0 0.739449704109194 0.0 58-59 3.961585299666197E-5 3.1692682397329577E-4 0.0 0.8652102294470974 0.0 60-61 3.961585299666197E-5 3.1692682397329577E-4 0.0 1.0050145746723174 0.0 62-63 3.961585299666197E-5 3.1692682397329577E-4 0.0 1.1472552948568322 0.0 64-65 3.961585299666197E-5 3.1692682397329577E-4 0.0 1.295656280182328 0.0 66-67 3.961585299666197E-5 3.1692682397329577E-4 0.0 1.4609336188844018 0.0 68-69 3.961585299666197E-5 3.1692682397329577E-4 0.0 1.6521989571522857 0.0 70-71 3.961585299666197E-5 3.1692682397329577E-4 0.0 1.8640447310519357 0.0 72-73 3.961585299666197E-5 3.1692682397329577E-4 0.0 2.080783062796673 0.0 74-75 3.961585299666197E-5 3.1692682397329577E-4 0.0 2.2983731353808388 0.0 76-77 3.961585299666197E-5 3.1692682397329577E-4 0.0 2.528580857144442 0.0 78-79 3.961585299666197E-5 3.1692682397329577E-4 0.0 2.7940863039280703 0.0 80-81 3.961585299666197E-5 3.1692682397329577E-4 0.0 3.0880161252368037 0.0 82-83 3.961585299666197E-5 3.1692682397329577E-4 0.0 3.3963265011833257 0.0 84-85 3.961585299666197E-5 3.1692682397329577E-4 0.0 3.6917617249059322 0.0 86-87 3.961585299666197E-5 3.1692682397329577E-4 0.0 3.9939316436379713 0.0 88 3.961585299666197E-5 3.1692682397329577E-4 0.0 4.246344051006203 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2660 0.0 33.21804 1 GTATCAA 3880 0.0 31.25257 1 TCAACGC 3900 0.0 29.646147 4 GTACATG 8190 0.0 29.037846 1 CAACGCA 3990 0.0 28.859642 5 ATCAACG 4010 0.0 28.832912 3 AACGCAG 4110 0.0 28.245737 6 TACATGG 8255 0.0 27.385822 2 ACATGGG 8430 0.0 26.37129 3 TATCAAC 4745 0.0 24.861954 2 ACGCAGA 4780 0.0 24.18828 7 CGCAGAG 5090 0.0 22.438108 8 GAGTACA 5730 0.0 21.900518 1 TACACCG 410 0.0 21.780483 5 CATGGGG 5780 0.0 21.548437 4 ACACCGT 475 0.0 20.778942 6 AGTACAT 6050 0.0 18.566938 2 GCAGAGT 6010 0.0 18.3777 9 CATGGGA 2815 0.0 17.865005 4 TAGACTG 980 0.0 16.785711 5 >>END_MODULE