FastQCFastQC Report
Wed 25 May 2016
SRR1032941_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032941_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2524242
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57350.22719691693585642No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47280.18730375296821777No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25980.10292198608532781No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39650.051.184381
GTATCAA72450.040.3967631
ATCAACG87450.032.869233
TCAACGC88100.032.6267244
CAACGCA89100.032.3127025
AACGCAG91800.031.4648176
TATCAAC94200.030.7630482
ACGCAGA108150.026.7074727
GTACATG85300.026.1051521
CGCAGAG110500.026.0963958
TACATGG84250.025.6854672
ACATGGG85300.024.0461543
GCAGAGT122550.023.152799
TACCGTC1754.4069984E-621.505177
GCATACG1554.2562162E-521.2159881
GAGTACT75750.020.61959512-13
AGAGTAC110350.020.3482310-11
CATGGGG56100.019.8743844
CAGAGTA119450.019.74188610-11
CTAGTAC5050.019.5630283
Produced by FastQC (version 0.11.5)