##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032941_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2524242 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.81928198643395 34.0 31.0 34.0 31.0 34.0 2 32.87681133583864 34.0 31.0 34.0 31.0 34.0 3 32.95791172161782 34.0 31.0 34.0 31.0 34.0 4 36.369414263767105 37.0 37.0 37.0 35.0 37.0 5 36.35127535315552 37.0 37.0 37.0 35.0 37.0 6 36.41035011698561 37.0 37.0 37.0 35.0 37.0 7 36.376174709080985 37.0 37.0 37.0 35.0 37.0 8 36.318185419623 37.0 37.0 37.0 35.0 37.0 9 38.160499666830674 39.0 39.0 39.0 37.0 39.0 10-11 38.170280226697756 39.0 39.0 39.0 37.0 39.0 12-13 38.159450044805524 39.0 39.0 39.0 37.0 39.0 14-15 39.71430116446838 41.0 40.0 41.0 37.0 41.0 16-17 39.727431640864864 41.0 40.0 41.0 37.0 41.0 18-19 39.74835455554579 41.0 40.0 41.0 37.0 41.0 20-21 39.75828426909939 41.0 40.0 41.0 37.0 41.0 22-23 39.72173428696615 41.0 40.0 41.0 37.0 41.0 24-25 39.700405705950544 41.0 40.0 41.0 37.0 41.0 26-27 39.62362701357477 41.0 40.0 41.0 37.0 41.0 28-29 39.56400079707096 41.0 40.0 41.0 37.0 41.0 30-31 39.45504749544615 41.0 39.5 41.0 36.0 41.0 32-33 39.334964119921935 41.0 39.0 41.0 35.5 41.0 34-35 39.246412982590414 41.0 39.0 41.0 35.0 41.0 36-37 39.159849966841534 41.0 39.0 41.0 35.0 41.0 38-39 39.06953552789312 41.0 39.0 41.0 35.0 41.0 40-41 38.971378338526975 41.0 39.0 41.0 35.0 41.0 42-43 38.83554726527805 41.0 39.0 41.0 35.0 41.0 44-45 38.66394684027918 41.0 39.0 41.0 35.0 41.0 46-47 38.44606460077917 41.0 38.0 41.0 35.0 41.0 48-49 38.25051460993043 41.0 38.0 41.0 34.5 41.0 50-51 38.09423581415728 40.0 38.0 41.0 34.0 41.0 52-53 37.95301341947405 40.0 38.0 41.0 34.0 41.0 54-55 37.80120705542495 40.0 37.0 41.0 34.0 41.0 56-57 37.58804425249243 40.0 37.0 41.0 33.0 41.0 58-59 37.41751167281109 40.0 36.5 41.0 33.0 41.0 60-61 37.22365981550105 40.0 36.0 41.0 33.0 41.0 62-63 36.98525141408787 39.0 35.5 41.0 33.0 41.0 64-65 36.693362205366995 39.0 35.0 41.0 32.0 41.0 66-67 36.4163921288054 38.5 35.0 41.0 32.0 41.0 68-69 36.12118231928635 37.5 35.0 40.0 32.0 41.0 70-71 35.796434533614445 37.0 35.0 40.0 31.5 41.0 72-73 35.455449200195545 36.5 35.0 39.0 31.0 41.0 74-75 35.10792051633718 36.0 35.0 39.0 31.0 41.0 76-77 34.75945452139692 35.5 35.0 38.5 31.0 40.0 78-79 34.41621247091206 35.0 35.0 37.0 31.0 39.0 80-81 34.08578060265221 35.0 34.0 37.0 30.0 39.0 82-83 33.78284193829276 35.0 34.0 36.5 30.0 39.0 84-85 33.50868221034275 35.0 34.0 36.0 30.0 37.0 86-87 33.366155067541065 35.0 34.0 36.0 30.0 37.0 88-89 33.182846771426824 35.0 34.0 35.5 30.0 37.0 90-91 33.01556031474003 35.0 34.0 35.0 30.0 36.0 92-93 32.85722822930607 35.0 34.0 35.0 30.0 36.0 94-95 32.76233716894022 35.0 34.0 35.0 30.0 36.0 96-97 32.70095834709984 35.0 34.0 35.0 30.0 36.0 98-99 32.593030303750595 35.0 34.0 35.0 30.0 36.0 100 32.51042768482578 35.0 34.0 35.0 30.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19422055573524943 1101 2 0.25233029131243256 1101 3 0.20406306925961104 1101 4 0.12690870492831863 1101 5 0.1245729096633923 1101 6 0.11632660819556406 1101 7 0.11431981840938477 1101 8 0.07219494283520334 1101 9 0.13230531578275162 1101 10-11 0.21349856325554128 1101 12-13 0.21260074311047106 1101 14-15 0.30925750330909807 1101 16-17 0.2855107270094237 1101 18-19 0.2584232746703776 1101 20-21 0.2931611873534692 1101 22-23 0.2732961746338418 1101 24-25 0.2659194310174229 1101 26-27 0.3479397226786318 1101 28-29 0.3094107021354091 1101 30-31 0.31716065866406495 1101 32-33 0.3790730154323825 1101 34-35 0.28855038949116363 1101 36-37 0.3000690176493279 1101 38-39 0.34368002053975033 1101 40-41 0.377843569303721 1101 42-43 0.4025458056762048 1101 44-45 0.3981616988565122 1101 46-47 0.45114484588267345 1101 48-49 0.3587786613595725 1101 50-51 0.324680264463332 1101 52-53 0.4024010987424731 1101 54-55 0.3939177688912423 1101 56-57 0.4372168995700676 1101 58-59 0.45167539890467623 1101 60-61 0.4774555579909787 1101 62-63 0.3992849172024293 1101 64-65 0.4228943977740869 1101 66-67 0.4346503833511477 1101 68-69 0.3977972099314684 1101 70-71 0.48433767495615854 1101 72-73 0.4465755081112235 1101 74-75 0.4443905413641147 1101 76-77 0.4024098989874645 1101 78-79 0.4196843436647555 1101 80-81 0.4236502901482879 1101 82-83 0.3991474089714515 1101 84-85 0.4564784967443387 1101 86-87 0.5100685974607799 1101 88-89 0.4500880231427331 1101 90-91 0.46703154761472376 1101 92-93 0.4996411169444386 1101 94-95 0.5212547245399008 1101 96-97 0.5005826444121837 1101 98-99 0.4922142676432415 1101 100 0.5155452308747641 1102 1 0.16249187424060096 1102 2 0.2337802410186427 1102 3 0.18903132994518046 1102 4 0.12849054557463546 1102 5 0.13586423722799879 1102 6 0.11463588628299703 1102 7 0.14184006883629507 1102 8 0.059887787081834176 1102 9 0.13501560867761242 1102 10-11 0.17598024802300927 1102 12-13 0.17576931555372965 1102 14-15 0.2430946109803358 1102 16-17 0.2285426024254349 1102 18-19 0.25048166224941326 1102 20-21 0.2511077972544271 1102 22-23 0.21090916552991956 1102 24-25 0.20336259265718581 1102 26-27 0.22529981982113867 1102 28-29 0.22976150396512907 1102 30-31 0.24425189138288772 1102 32-33 0.27661204362454583 1102 34-35 0.27083970317766415 1102 36-37 0.317380926325157 1102 38-39 0.31389874003650675 1102 40-41 0.27910910136521494 1102 42-43 0.23791214222564605 1102 44-45 0.3207617069375388 1102 46-47 0.40259468925953 1102 48-49 0.37132216601786183 1102 50-51 0.4218211104622611 1102 52-53 0.44279370927716144 1102 54-55 0.39470409578598264 1102 56-57 0.44010701668123176 1102 58-59 0.39611065176653426 1102 60-61 0.4106759128333195 1102 62-63 0.4313912380576639 1102 64-65 0.430206285184056 1102 66-67 0.41911961907696593 1102 68-69 0.4151579875818925 1102 70-71 0.38808735862630783 1102 72-73 0.42401373606861625 1102 74-75 0.392906374869348 1102 76-77 0.4174227116755347 1102 78-79 0.3776245312722821 1102 80-81 0.38874831518672437 1102 82-83 0.4087564359089271 1102 84-85 0.4016080378055449 1102 86-87 0.3624762131949737 1102 88-89 0.3802210411740603 1102 90-91 0.3468063845586471 1102 92-93 0.3901435657096286 1102 94-95 0.4061964028850724 1102 96-97 0.3756018368619536 1102 98-99 0.4261520054222814 1102 100 0.40618735388932947 1103 1 0.10469600864429651 1103 2 0.1277680154783596 1103 3 0.14937829622634524 1103 4 0.11451732629755895 1103 5 0.09832203024761554 1103 6 0.11701293463018914 1103 7 0.09182122434703643 1103 8 0.029147566599270647 1103 9 0.06464081272459765 1103 10-11 0.07446801333491493 1103 12-13 0.1094141487688205 1103 14-15 0.1046403063257273 1103 16-17 0.19691894740766003 1103 18-19 0.2222820529625551 1103 20-21 0.15392650188383072 1103 22-23 0.1948719156463028 1103 24-25 0.15512277366185856 1103 26-27 0.12289122169257638 1103 28-29 0.102327424102711 1103 30-31 0.09311725797630288 1103 32-33 0.18875244828301874 1103 34-35 0.18610508115502 1103 36-37 0.18388164493648418 1103 38-39 0.20753731208364457 1103 40-41 0.21852579632232505 1103 42-43 0.18788829693340858 1103 44-45 0.08008936320754856 1103 46-47 0.11216724257601385 1103 48-49 0.1349308348894951 1103 50-51 0.11580294900950605 1103 52-53 0.1137382308568462 1103 54-55 0.15058038486492364 1103 56-57 0.1921732630907158 1103 58-59 0.14305945045575186 1103 60-61 0.12555915628094994 1103 62-63 0.16825317320577682 1103 64-65 0.1961777318187714 1103 66-67 0.11254014798303302 1103 68-69 0.13198052616024114 1103 70-71 0.13976939700238944 1103 72-73 0.06724108354920588 1103 74-75 0.07035474564433031 1103 76-77 0.15129636211941033 1103 78-79 0.135839041325724 1103 80-81 0.05889372471386878 1103 82-83 0.0796056982904858 1103 84-85 0.12967506482817726 1103 86-87 0.09064333759096144 1103 88-89 0.0901334458081422 1103 90-91 0.039048756809471286 1103 92-93 0.07810647032236062 1103 94-95 0.12368750409848417 1103 96-97 0.08811014612299317 1103 98-99 0.10311407860069721 1103 100 0.09053292970332905 1104 1 0.09632489150879309 1104 2 0.13390445963538156 1104 3 0.09955900822096453 1104 4 0.027855806503964686 1104 5 0.051969512003999796 1104 6 0.0712753613138517 1104 7 0.03264447970091311 1104 8 0.019829992313461275 1104 9 0.1295841045085595 1104 10-11 0.09440032367796647 1104 12-13 0.1142961509891478 1104 14-15 0.1580445924177809 1104 16-17 0.1085209293872964 1104 18-19 0.10090518953636973 1104 20-21 0.11392288409708584 1104 22-23 0.146813362674294 1104 24-25 0.1455423384003467 1104 26-27 0.17868352290051348 1104 28-29 0.16596350470762644 1104 30-31 0.18506342625541095 1104 32-33 0.2753574155278571 1104 34-35 0.30749703839735787 1104 36-37 0.23539463251501758 1104 38-39 0.23815588662049691 1104 40-41 0.22766874737225606 1104 42-43 0.3087594978139805 1104 44-45 0.3228784403290632 1104 46-47 0.35435331583106944 1104 48-49 0.3332129379191642 1104 50-51 0.2586594556856596 1104 52-53 0.30724189967881443 1104 54-55 0.31740319173934495 1104 56-57 0.3169413483790535 1104 58-59 0.26792767513528304 1104 60-61 0.2926184592146299 1104 62-63 0.2398465323995893 1104 64-65 0.2766891323790972 1104 66-67 0.21353941378513497 1104 68-69 0.22051383679374226 1104 70-71 0.24531060095295487 1104 72-73 0.2089421448060591 1104 74-75 0.20222417585868868 1104 76-77 0.29784754335830854 1104 78-79 0.19580499945003993 1104 80-81 0.22015916976666716 1104 82-83 0.26646611753600524 1104 84-85 0.25734467025690577 1104 86-87 0.27093672512015843 1104 88-89 0.27706901885886026 1104 90-91 0.2659095622675238 1104 92-93 0.2901395521840513 1104 94-95 0.30231351756153657 1104 96-97 0.31651170311121035 1104 98-99 0.3804126638411489 1104 100 0.36836870504271246 1105 1 0.059118338350486965 1105 2 0.1348069795300333 1105 3 0.11697036746821254 1105 4 0.08381454065730054 1105 5 0.06997035087009351 1105 6 0.09552995831143818 1105 7 0.06564033424744053 1105 8 -0.01883855214018837 1105 9 0.0769997035532981 1105 10-11 0.07917456298667958 1105 12-13 0.09797042996782324 1105 14-15 0.16454557734660824 1105 16-17 0.17314953790395293 1105 18-19 0.1644508878469253 1105 20-21 0.1494520460607589 1105 22-23 0.15503652379000954 1105 24-25 0.19423010402706353 1105 26-27 0.14420623991733805 1105 28-29 0.16539050687526213 1105 30-31 0.18564313046985603 1105 32-33 0.16532421325314317 1105 34-35 0.15833027795500243 1105 36-37 0.17112941897358525 1105 38-39 0.15703708022010687 1105 40-41 0.05932639452334598 1105 42-43 0.0998209134971404 1105 44-45 0.027017983090217967 1105 46-47 0.02072035498611058 1105 48-49 0.032486261636229585 1105 50-51 0.07981297613842742 1105 52-53 0.053227220658762064 1105 54-55 0.03710983867333084 1105 56-57 0.10230336229422932 1105 58-59 0.12231052316525393 1105 60-61 0.048198794283663915 1105 62-63 0.062386568932325304 1105 64-65 0.05416728264525261 1105 66-67 0.10589436553896547 1105 68-69 0.08092901867877345 1105 70-71 0.07396060623848655 1105 72-73 0.08077568103587396 1105 74-75 0.0977797768812394 1105 76-77 0.0969503658358235 1105 78-79 0.07380999854638048 1105 80-81 0.12905660645102301 1105 82-83 0.1077764081951571 1105 84-85 0.15099940979353477 1105 86-87 0.16200246016804698 1105 88-89 0.14455681949517185 1105 90-91 0.12370220775338936 1105 92-93 0.13492397610014706 1105 94-95 0.1531758559974037 1105 96-97 0.1969852191536674 1105 98-99 0.1656053131917048 1105 100 0.16597236695466933 1106 1 0.0801618002986828 1106 2 0.0803557267551156 1106 3 0.0837122030980737 1106 4 0.034320998262188596 1106 5 0.057584242303576616 1106 6 0.062178576876647185 1106 7 0.0630283688643658 1106 8 0.05095692642562 1106 9 0.06455253926141324 1106 10-11 0.0909979933136782 1106 12-13 0.054400563414873204 1106 14-15 0.07989322980841251 1106 16-17 0.08025499413686532 1106 18-19 0.08294643121456602 1106 20-21 0.044659585433720395 1106 22-23 0.07246671371905222 1106 24-25 0.14508855768954732 1106 26-27 0.0841164800151617 1106 28-29 0.10429960731462273 1106 30-31 0.11880268367262659 1106 32-33 0.15162802015385068 1106 34-35 0.16784353677567765 1106 36-37 0.15535315677169592 1106 38-39 0.1880018434436863 1106 40-41 0.2228267301483413 1106 42-43 0.1951305342677614 1106 44-45 0.20157441433442358 1106 46-47 0.28584282725367416 1106 48-49 0.22129067437526118 1106 50-51 0.29345752233757594 1106 52-53 0.31618903897812345 1106 54-55 0.33961921446602616 1106 56-57 0.3960783935081551 1106 58-59 0.28256270872064704 1106 60-61 0.2938035443465026 1106 62-63 0.29516696680656906 1106 64-65 0.30647723635470214 1106 66-67 0.33764193430869227 1106 68-69 0.3283621372498686 1106 70-71 0.4042619892051178 1106 72-73 0.26610971828871754 1106 74-75 0.27844875016205606 1106 76-77 0.34315699757658535 1106 78-79 0.34539639181698334 1106 80-81 0.3785102629775068 1106 82-83 0.34671236298981256 1106 84-85 0.33076314664204887 1106 86-87 0.37216806420374127 1106 88-89 0.3771852314466386 1106 90-91 0.40258711967125294 1106 92-93 0.34377837031917835 1106 94-95 0.3650050652016148 1106 96-97 0.45011648489767 1106 98-99 0.37972697975601477 1106 100 0.4137870293600727 1107 1 0.08420937163784714 1107 2 0.057946024525897144 1107 3 0.020837479725273056 1107 4 0.03843161220690661 1107 5 0.047739320170222754 1107 6 0.03380412768363783 1107 7 -0.03009680722793462 1107 8 -0.009705473617479754 1107 9 -0.005853345418664446 1107 10-11 -0.005463684905478772 1107 12-13 -0.004276515279656223 1107 14-15 -0.01558937869464927 1107 16-17 0.009071526129815766 1107 18-19 0.056902128370666105 1107 20-21 0.05865601588918423 1107 22-23 0.04892845859117756 1107 24-25 0.07928592881289376 1107 26-27 0.10285086082777184 1107 28-29 0.10265477158018399 1107 30-31 0.16086706378121107 1107 32-33 0.19664512705647752 1107 34-35 0.14421363380712648 1107 36-37 0.15507099251070855 1107 38-39 0.13374035076974877 1107 40-41 0.0957610954363588 1107 42-43 0.1687145369427654 1107 44-45 0.17126855632942295 1107 46-47 0.13078016581981444 1107 48-49 0.1425319916102339 1107 50-51 0.08935101171663007 1107 52-53 0.0775193504322047 1107 54-55 0.16110557164348904 1107 56-57 0.17726827130963585 1107 58-59 0.17607368056407324 1107 60-61 0.2076095833559819 1107 62-63 0.24377408626077823 1107 64-65 0.17834331414233873 1107 66-67 0.1749478543842784 1107 68-69 0.18356080546787723 1107 70-71 0.145633240860505 1107 72-73 0.202863404580107 1107 74-75 0.18653450587974163 1107 76-77 0.14579326854259023 1107 78-79 0.16133503442081576 1107 80-81 0.14836559408379912 1107 82-83 0.2017736635920997 1107 84-85 0.2145396597721998 1107 86-87 0.12899353713783768 1107 88-89 0.1571968790540339 1107 90-91 0.1135896095764437 1107 92-93 0.11403316797949259 1107 94-95 0.07233605235023077 1107 96-97 0.15677885939046377 1107 98-99 0.1261736124546573 1107 100 0.051713072052130826 1108 1 0.0519487244246406 1108 2 0.08451058482140894 1108 3 0.06214584677808688 1108 4 0.005878373730332953 1108 5 0.034234437919252514 1108 6 0.01725516582736475 1108 7 0.02445776479981987 1108 8 0.0961483266409715 1108 9 0.09243032088020442 1108 10-11 0.03214875575585552 1108 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-0.12178641376986121 2214 32-33 -0.18107633842882365 2214 34-35 -0.23873782455304138 2214 36-37 -0.17849905977890757 2214 38-39 -0.1480098958296736 2214 40-41 -0.19074764982418202 2214 42-43 -0.16141690598443859 2214 44-45 -0.17606476840451535 2214 46-47 -0.2586856285854964 2214 48-49 -0.2066477881027211 2214 50-51 -0.29598091814835925 2214 52-53 -0.21256217830930524 2214 54-55 -0.22244893400057464 2214 56-57 -0.2129108311126302 2214 58-59 -0.14982959898004822 2214 60-61 -0.25474291419981654 2214 62-63 -0.20322213041782078 2214 64-65 -0.20127084279391028 2214 66-67 -0.24799712144582742 2214 68-69 -0.17021973880195418 2214 70-71 -0.17447725665041247 2214 72-73 -0.16262633478107347 2214 74-75 -0.1306907474328085 2214 76-77 -0.13204381168252155 2214 78-79 -0.18218121923047192 2214 80-81 -0.22654813955607978 2214 82-83 -0.21165678871427218 2214 84-85 -0.23701933098633532 2214 86-87 -0.27341802765810286 2214 88-89 -0.2133813165679399 2214 90-91 -0.19473880413737277 2214 92-93 -0.19813051690593397 2214 94-95 -0.2327171699519539 2214 96-97 -0.24590326239123073 2214 98-99 -0.24542340529879425 2214 100 -0.24884602407843914 2215 1 -0.18936760655857654 2215 2 -0.20838264507752058 2215 3 -0.23440177057860723 2215 4 -0.1318209240204169 2215 5 -0.1512766271311392 2215 6 -0.17065537484915438 2215 7 -0.1590715626809498 2215 8 -0.1368688699405496 2215 9 -0.17084855164127077 2215 10-11 -0.18368108327013033 2215 12-13 -0.18453779582107188 2215 14-15 -0.28272237239958287 2215 16-17 -0.2975604010881767 2215 18-19 -0.2736752209550559 2215 20-21 -0.3578940589656625 2215 22-23 -0.2724125617886415 2215 24-25 -0.3065289131079112 2215 26-27 -0.2902332270997192 2215 28-29 -0.28516460511444564 2215 30-31 -0.2863106848660877 2215 32-33 -0.3296503689525778 2215 34-35 -0.3601362359472233 2215 36-37 -0.35424101556417753 2215 38-39 -0.35442469716774383 2215 40-41 -0.3537663537427349 2215 42-43 -0.31186137347428655 2215 44-45 -0.27948604356866724 2215 46-47 -0.3060273019381441 2215 48-49 -0.3536017957634101 2215 50-51 -0.342543631217751 2215 52-53 -0.3377883517156519 2215 54-55 -0.4256156448840116 2215 56-57 -0.41211550068404534 2215 58-59 -0.3669562946321321 2215 60-61 -0.35457100261243113 2215 62-63 -0.3404050008300672 2215 64-65 -0.36032941007137254 2215 66-67 -0.3080250308895529 2215 68-69 -0.3358955087764457 2215 70-71 -0.34687676558615266 2215 72-73 -0.3553366028068723 2215 74-75 -0.3759925382795899 2215 76-77 -0.4079716833987206 2215 78-79 -0.39901512185156207 2215 80-81 -0.31840282439243595 2215 82-83 -0.3736126862596194 2215 84-85 -0.3775228949013112 2215 86-87 -0.4742656513280039 2215 88-89 -0.4989166992637024 2215 90-91 -0.49180200708311617 2215 92-93 -0.4199162882422698 2215 94-95 -0.38874523036591313 2215 96-97 -0.40738885658815605 2215 98-99 -0.49897485218957627 2215 100 -0.48542860737659055 2216 1 -0.17633898575759588 2216 2 -0.20005648876071547 2216 3 -0.18726107546104487 2216 4 -0.13127947940956375 2216 5 -0.17327456052041157 2216 6 -0.1590593667196245 2216 7 -0.15201381876492093 2216 8 -0.09962607463214823 2216 9 -0.14154560260440974 2216 10-11 -0.18711989947405527 2216 12-13 -0.2120636816468391 2216 14-15 -0.2758814884748304 2216 16-17 -0.29346651831144754 2216 18-19 -0.263739161928072 2216 20-21 -0.22676749247195005 2216 22-23 -0.30248928725596613 2216 24-25 -0.302818888935775 2216 26-27 -0.3058353055513692 2216 28-29 -0.26946187883491035 2216 30-31 -0.2964219293931265 2216 32-33 -0.38165095267297744 2216 34-35 -0.3554039378927456 2216 36-37 -0.3933013228545761 2216 38-39 -0.34946614964170664 2216 40-41 -0.35172455806707603 2216 42-43 -0.3276421758261847 2216 44-45 -0.29866967682573886 2216 46-47 -0.3450539366096521 2216 48-49 -0.38816084171821075 2216 50-51 -0.3140044215807194 2216 52-53 -0.3031486163602395 2216 54-55 -0.3416623591672163 2216 56-57 -0.4347671592918019 2216 58-59 -0.38974317563179994 2216 60-61 -0.42666327021354533 2216 62-63 -0.4321548623005427 2216 64-65 -0.4094684687366552 2216 66-67 -0.5028831803571236 2216 68-69 -0.4729659400542374 2216 70-71 -0.45890836696894866 2216 72-73 -0.4032614814143045 2216 74-75 -0.4170924210729794 2216 76-77 -0.36840071326651724 2216 78-79 -0.40768175089863234 2216 80-81 -0.4316190729111611 2216 82-83 -0.3814766997582737 2216 84-85 -0.46061209403877257 2216 86-87 -0.4582125077947623 2216 88-89 -0.42331452367949396 2216 90-91 -0.41941121876455156 2216 92-93 -0.443193781149283 2216 94-95 -0.49210768188058296 2216 96-97 -0.47424840846480265 2216 98-99 -0.44702806140292495 2216 100 -0.49153366594752157 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 3.0 4 8.0 5 13.0 6 46.0 7 156.0 8 305.0 9 573.0 10 885.0 11 1494.0 12 2411.0 13 3654.0 14 4329.0 15 5856.0 16 7342.0 17 10621.0 18 13080.0 19 7363.0 20 5203.0 21 4812.0 22 5119.0 23 5361.0 24 5975.0 25 7119.0 26 8879.0 27 11360.0 28 14699.0 29 19008.0 30 23941.0 31 30132.0 32 38812.0 33 50883.0 34 71765.0 35 113023.0 36 217270.0 37 497883.0 38 891015.0 39 442442.0 40 1393.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.65927460088946 19.410927178815943 13.334162626503524 23.595635593791076 2 15.790399448544704 19.891927439258705 40.093137313160845 24.224535799035745 3 18.476189646142718 25.22943557620161 29.94721575382353 26.347159023832145 4 12.370995144092403 16.467335146374552 38.25005863857086 32.91161107096218 5 12.641706916660327 38.018258284526254 35.49558200856976 13.844452790243656 6 31.266069816991276 37.83792670935312 17.251446234007435 13.644557239648167 7 27.84060229131532 32.57575487466064 21.934833911530145 17.6488089224939 8 25.834799215762814 36.21467501049326 19.65440879513589 18.296116978608033 9 25.66975357061927 16.163024341854012 20.658922467684963 37.50829961984176 10-11 24.45973880475802 26.479275758821856 28.36861521201216 20.69237022440796 12-13 25.19675244568856 23.81425602498655 28.837058946105714 22.15193258321917 14-15 22.190216970385325 25.58698395575537 26.17492364528946 26.047875428569846 16-17 21.559660286137383 29.25068198690934 26.748940870170134 22.440716856783144 18-19 21.462547722303633 27.72908983658045 29.468406121607345 21.339956319508573 20-21 22.341479145026508 26.9795645583902 29.25779303252224 21.421163264061054 22-23 22.145244141849457 26.6616935516618 29.359753700074815 21.833308606413933 24-25 21.849721241614766 26.866282734252717 29.313148688173698 21.970847335958823 26-27 21.637194287913665 26.970133551286775 29.34182326952741 22.05084889127215 28-29 21.78249340443091 27.021488936598942 29.389257631506275 21.806760027463877 30-31 21.923667055948634 27.220417789690664 29.033025572754624 21.82288958160608 32-33 21.68845744285679 27.054466209355887 29.400658725638294 21.85641762214903 34-35 21.89219401415999 26.837135524049284 29.395951559465505 21.87471890232522 36-37 22.067001744744218 27.117961948525586 28.920586891927908 21.894449414802285 38-39 21.89423125130417 27.355210568670856 28.98239922192667 21.7681589580983 40-41 21.79312649246033 27.083766451126774 29.158728513476312 21.96437854293658 42-43 21.815992388352594 27.06854447065353 28.964558266764456 22.15090487422942 44-45 21.876214574095957 27.222462382905142 28.884059235522276 22.01726380747662 46-47 22.031580066268084 27.374037887195122 28.545528966704925 22.04885307983186 48-49 22.090287095401404 27.4101208198601 28.332282973018103 22.167309111720396 50-51 22.067946343829583 27.47052825254143 28.231342161802203 22.23018324182678 52-53 22.125890660978918 27.46860724934383 28.136875644968235 22.26862644470902 54-55 22.070491832537773 27.729134151831307 28.047563048207564 22.15281096742336 56-57 22.010107282811195 27.458594164519347 28.194929227606586 22.336369325062876 58-59 21.964602794566307 27.097669450024465 28.495706005089826 22.4420217503194 60-61 22.094296789031752 27.496158535980886 28.146064853826097 22.263479821161262 62-63 21.904594983303983 27.440530973381144 28.254196606550963 22.40067743676391 64-65 21.936391585353462 27.24226267573843 28.32468684157201 22.49665889733609 66-67 21.819667773401985 27.323599450290203 28.26326804770226 22.59346472860555 68-69 22.04311000538776 27.389630146103382 28.218533103032996 22.348726745475865 70-71 22.050778015737002 27.285973373392885 28.1545509503447 22.508697660525417 72-73 21.904773789517805 27.388459585095248 28.233029955131084 22.47373667025586 74-75 21.963405251952864 27.53678133871475 27.982935075163155 22.516878334169228 76-77 22.02856224900871 27.452588718747535 27.88454034301796 22.63430868922579 78-79 22.059671774734753 27.491282531548084 27.974477090548373 22.474568603168795 80-81 22.00055779253604 27.354870939167338 28.02491526148902 22.619656006807602 82-83 21.96191049300611 27.397153636631405 28.06068047848886 22.580255391873628 84-85 21.913766588148047 27.453528623642264 27.893502287023193 22.739202501186494 86-87 21.995102647509864 27.45286565637478 27.900211590031144 22.651820106084212 88-89 21.737093656351522 27.40194201526919 27.95187133044546 22.90909299793383 90-91 21.791174562615442 27.38373469215951 27.929661927611804 22.89542881761324 92-93 21.906727060348057 27.468217353074646 27.81216579963161 22.812889786945686 94-95 21.715979027143092 27.272558003181185 27.922007435975143 23.089455533700576 96-97 21.776586481285015 27.293210534364086 27.91947973127689 23.010723253074 98-99 21.708584877592905 27.375139695456973 27.79641484311522 23.119860583834903 100 21.768237752164808 27.145297479401737 27.841506479965073 23.24495828846838 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 81.0 1 76.0 2 244.5 3 420.0 4 267.0 5 175.0 6 453.0 7 1063.0 8 1858.0 9 2137.0 10 1606.0 11 1127.0 12 1106.0 13 1260.0 14 1468.0 15 1623.0 16 1741.5 17 1914.0 18 2180.0 19 2490.5 20 2890.5 21 3439.0 22 4236.5 23 5755.0 24 7932.0 25 10579.5 26 14009.5 27 18532.5 28 23967.0 29 30252.5 30 36851.5 31 43334.0 32 49591.5 33 55901.0 34 61859.0 35 66898.5 36 72458.5 37 78120.5 38 82318.0 39 84255.0 40 86431.5 41 89195.5 42 90428.0 43 90655.0 44 89417.5 45 88691.0 46 89323.5 47 87921.5 48 85858.0 49 83806.5 50 80950.0 51 78280.0 52 74033.5 53 70031.5 54 67634.5 55 64046.0 56 58795.5 57 53556.0 58 49076.5 59 45405.5 60 40731.5 61 35139.0 62 30180.5 63 25608.5 64 21051.5 65 16865.0 66 13623.0 67 10598.5 68 8054.5 69 6133.5 70 4476.5 71 3345.0 72 2576.0 73 1893.0 74 1369.5 75 948.5 76 623.5 77 421.0 78 286.5 79 156.5 80 80.5 81 47.5 82 28.5 83 16.5 84 8.0 85 4.5 86 2.5 87 1.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.5846341198664789E-4 2 4.753902359599436E-4 3 1.5846341198664789E-4 4 0.012122451016978562 5 7.130853539399154E-4 6 0.003961585299666197 7 5.942377949499295E-4 8 0.1416662903160632 9 0.001188475589899859 10-11 0.0 12-13 1.5846341198664789E-4 14-15 1.386554854883169E-4 16-17 0.0 18-19 1.188475589899859E-4 20-21 0.0 22-23 9.903963249165493E-5 24-25 5.150060889566056E-4 26-27 0.006160265140980937 28-29 0.007883554746335732 30-31 0.014439978417283287 32-33 0.017015008862066316 34-35 0.025869152006820266 36-37 0.037615252420330536 38-39 0.04304262428087323 40-41 0.053580441177985313 42-43 0.07099160857001825 44-45 0.11021130303671359 46-47 0.11757985169409271 48-49 0.11429173589536977 50-51 0.11603483342722291 52-53 0.11092438839065352 54-55 0.11714407731112943 56-57 0.09888116907966826 58-59 0.0930180228361623 60-61 0.09575151669293198 62-63 0.09440457769104547 64-65 1.9807926498330986E-5 66-67 3.961585299666197E-5 68-69 7.923170599332394E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 3.961585299666197E-5 78-79 0.0 80-81 2.376951179799718E-4 82-83 1.386554854883169E-4 84-85 0.0 86-87 8.319329129299013E-4 88-89 3.3673475047162673E-4 90-91 1.7827133848497884E-4 92-93 0.0010498201044115423 94-95 0.0010696280309098732 96-97 3.1692682397329577E-4 98-99 3.961585299666197E-5 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2524242.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.692487526101125 #Duplication Level Percentage of deduplicated Percentage of total 1 77.04937209720566 47.53367427000784 2 13.671761856801822 16.868899956211433 3 4.140676508042422 7.663459014660814 4 1.7669788918542515 4.360372929784096 5 0.9217163507281362 2.8431487234949495 6 0.562776635571421 2.0831454341982627 7 0.37123749155189284 1.6031795021750357 8 0.2709977052043134 1.3374818038335308 9 0.19846924467030988 1.1019655261024024 >10 0.9863136515835113 10.722913136290712 >50 0.04616982653688645 1.9195260760515354 >100 0.013080348619472668 1.3201437603436248 >500 1.9259641277991895E-4 0.0746966129374967 >1k 1.925964127641178E-4 0.340253765484208 >5k 6.419880425470593E-5 0.22713948842415702 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5735 0.22719691693585642 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4728 0.18730375296821777 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2598 0.10292198608532781 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 3.961585299666197E-5 0.0 3.961585299666197E-5 0.0 6 0.0 3.961585299666197E-5 0.0 3.961585299666197E-5 0.0 7 0.0 3.961585299666197E-5 0.0 3.961585299666197E-5 0.0 8 0.0 3.961585299666197E-5 0.0 3.961585299666197E-5 0.0 9 0.0 3.961585299666197E-5 0.0 1.5846341198664789E-4 0.0 10-11 0.0 3.961585299666197E-5 0.0 1.5846341198664789E-4 0.0 12-13 0.0 3.961585299666197E-5 0.0 1.5846341198664789E-4 0.0 14-15 0.0 5.942377949499296E-5 0.0 1.5846341198664789E-4 0.0 16-17 0.0 1.1884755898998591E-4 0.0 1.9807926498330983E-4 0.0 18-19 0.0 1.5846341198664789E-4 0.0 2.376951179799718E-4 0.0 20-21 0.0 1.5846341198664789E-4 0.0 2.376951179799718E-4 0.0 22-23 0.0 1.5846341198664789E-4 0.0 3.763506034682887E-4 0.0 24-25 0.0 1.7827133848497884E-4 0.0 4.753902359599436E-4 0.0 26-27 0.0 1.9807926498330983E-4 0.0 6.140457214482605E-4 0.0 28-29 0.0 1.9807926498330983E-4 0.0 0.001485594487374824 0.0 30-31 0.0 1.9807926498330983E-4 0.0 0.004159664564649506 0.0 32-33 0.0 1.9807926498330983E-4 0.0 0.011944179678493583 0.0 34-35 0.0 1.9807926498330983E-4 0.0 0.022046222192642385 0.0 36-37 0.0 2.575030444783028E-4 0.0 0.04254742611841496 0.0 38-39 0.0 2.773109709766338E-4 0.0 0.08150961754063199 0.0 40-41 0.0 2.773109709766338E-4 0.0 0.12795920517921816 0.0 42-43 0.0 2.773109709766338E-4 0.0 0.17280435077143952 0.0 44-45 0.0 2.773109709766338E-4 0.0 0.22478034990306 0.0 46-47 0.0 2.773109709766338E-4 0.0 0.2899880439355656 0.0 48-49 0.0 2.773109709766338E-4 0.0 0.36642683229262485 0.0 50-51 0.0 2.773109709766338E-4 0.0 0.4512641814849765 0.0 52-53 0.0 2.773109709766338E-4 0.0 0.5390331037990811 0.0 54-55 0.0 2.773109709766338E-4 0.0 0.6331603705191499 0.0 56-57 0.0 2.773109709766338E-4 0.0 0.7413908809060304 0.0 58-59 0.0 2.773109709766338E-4 0.0 0.8674089092884121 0.0 60-61 0.0 2.773109709766338E-4 0.0 1.0076688368230937 0.0 62-63 0.0 2.773109709766338E-4 0.0 1.1501868679785852 0.0 64-65 0.0 2.773109709766338E-4 0.0 1.299776328893981 0.0 66-67 0.0 2.773109709766338E-4 0.0 1.4664402224509376 0.0 68-69 0.0 2.773109709766338E-4 0.0 1.6590128838677116 0.0 70-71 0.0 2.773109709766338E-4 0.0 1.872740410784703 0.0 72-73 0.0 2.9711889747496476E-4 0.0 2.09066721811934 0.0 74-75 0.0 3.1692682397329577E-4 0.0 2.3094655742199044 0.0 76-77 0.0 3.1692682397329577E-4 0.0 2.5405844606024304 0.0 78-79 0.0 3.1692682397329577E-4 0.0 2.806842608592995 0.0 80-81 0.0 3.1692682397329577E-4 0.0 3.102218408536107 0.0 82-83 0.0 3.1692682397329577E-4 0.0 3.4119747631170068 0.0 84-85 0.0 3.565426769699577E-4 0.0 3.7100840569168883 0.0 86-87 0.0 3.565426769699577E-4 0.0 4.013838609768794 0.0 88 0.0 3.565426769699577E-4 0.0 4.266706599446487 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3965 0.0 51.18438 1 GTATCAA 7245 0.0 40.396763 1 ATCAACG 8745 0.0 32.86923 3 TCAACGC 8810 0.0 32.626724 4 CAACGCA 8910 0.0 32.312702 5 AACGCAG 9180 0.0 31.464817 6 TATCAAC 9420 0.0 30.763048 2 ACGCAGA 10815 0.0 26.707472 7 GTACATG 8530 0.0 26.105152 1 CGCAGAG 11050 0.0 26.096395 8 TACATGG 8425 0.0 25.685467 2 ACATGGG 8530 0.0 24.046154 3 GCAGAGT 12255 0.0 23.15279 9 TACCGTC 175 4.4069984E-6 21.50517 7 GCATACG 155 4.2562162E-5 21.215988 1 GAGTACT 7575 0.0 20.619595 12-13 AGAGTAC 11035 0.0 20.34823 10-11 CATGGGG 5610 0.0 19.874384 4 CAGAGTA 11945 0.0 19.741886 10-11 CTAGTAC 505 0.0 19.563028 3 >>END_MODULE