FastQCFastQC Report
Wed 25 May 2016
SRR1032940_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032940_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3319429
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT41610.1253528844870609No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG95700.027.160221
TACATGG96650.025.6288342
ACATGGG95550.025.4319673
CATGGGG62050.019.4675564
GAGTACA70500.018.2676011
AACGCGC650.00804188518.07730340-41
GTCTCGC6300.017.9056761
GTATCAA95700.016.600641
GTATAGG13050.016.2077261
TCAACGC94050.015.99231154
ATCAACG95550.015.9380223
CATGGGC31850.015.938024
CATGGGA35050.015.823924
CAACGCA95100.015.8152645
AGAGTAC63150.015.70302710-11
AGTACAT72950.015.5278682
AACGCAG97150.015.481546
GGTATCA76200.015.4207361
TATACTG22950.015.3597995
GTCTAGG9900.015.1926981
Produced by FastQC (version 0.11.5)