FastQCFastQC Report
Wed 25 May 2016
SRR1032940_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032940_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3319429
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71270.2147056014754345No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60860.18334478610628513No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT44520.13411945247209683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG91250.027.3403321
TACATGG91950.026.0921882
GTATCAA149350.025.5442371
GGTATCA107150.025.4756971
ACATGGG91050.025.265483
TACACCG4000.024.6940565
TCAACGC162550.023.123764
ATCAACG164100.022.9340133
CAACGCA165400.022.7787655
AACGCAG168650.022.33986
TATCAAC171600.021.9861492
TATAACG1805.625643E-620.9078582
GAGTACT106850.020.68405212-13
AGAGTAC141250.019.80358510-11
ACGCAGA189750.019.73147
CGCAGAG192050.019.4942138
CATGGGG58300.018.6398354
GTACTTT119800.018.38935714-15
GAGTACA69900.018.2824271
GCAGAGT205450.018.0167799
Produced by FastQC (version 0.11.5)