FastQCFastQC Report
Wed 25 May 2016
SRR1032939_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032939_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3444267
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27450.033.217981
TCAACGC39750.030.6245784
GTATCAA43100.029.9895041
GTACATG98500.029.4894081
CAACGCA42400.028.5992775
TACATGG97550.028.4752622
ATCAACG43050.028.2770523
AACGCAG43500.028.0921736
ACATGGG101250.026.8776343
TATCAAC52500.023.8138642
TATACCG2002.3013854E-823.5001835
ACGCAGA52800.022.6100227
CATGGGG67700.021.8690244
CGCAGAG55600.020.5415278
TACACCG3503.6379788E-1220.1430135
GAGTACA70100.019.9807641
CATGGGA36250.018.4114444
TCTGTCG8250.018.2304448
AGTACAT71100.018.0468162
CTGTCGC8200.017.1952559
Produced by FastQC (version 0.11.5)