FastQCFastQC Report
Wed 25 May 2016
SRR1032939_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032939_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3444267
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59490.17272180118440295No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47530.1379974316741414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44400.051.7456931
GTATCAA86500.044.4309431
TCAACGC97600.038.7023854
ATCAACG98600.038.3098643
CAACGCA100400.037.572365
AACGCAG106000.035.7205246
TATCAAC106800.035.544652
ACGCAGA124850.030.0632427
CGCAGAG126400.029.5825238
GTACATG96450.027.7177731
GCAGAGT140850.026.3488589
TACATGG98100.025.7979552
ACATGGG100600.024.0813753
GAGTACT93800.021.88650712-13
CAGAGTA139300.021.11098310-11
AGAGTAC129750.020.81832310-11
GTGGTAT22950.020.2675291
TGGTATC21700.020.1602172
CATGGGG66000.020.0991274
AGTACTT100600.019.61323412-13
Produced by FastQC (version 0.11.5)