FastQCFastQC Report
Wed 25 May 2016
SRR1032938_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032938_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3769188
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG114250.028.3856331
TACATGG116200.026.372252
ACATGGG117350.024.7920973
GTATCAA72150.023.4515671
TCAACGC71700.022.2221584
CAACGCA73850.021.7022045
ATCAACG74100.021.5658423
AACGCAG76150.021.108446
GGTATCA54950.020.9557911
CATGGGG77550.019.6973254
GAGTACA83000.019.5931261
TATCAAC84700.019.4773312
ACGCAGA87100.018.2386537
TAGACCG1550.001033886318.1936345
AGTACAT83000.018.1774392
CGCAGAG88600.017.8768238
GTCGTAC2859.598298E-716.4915281
CATGGGA43650.016.366854
TACACTG29350.016.333985
GTATAGG15350.015.6159171
Produced by FastQC (version 0.11.5)