##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1032938_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3769188
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.748813802866824	33.0	31.0	34.0	30.0	34.0
2	31.863894557660696	34.0	31.0	34.0	30.0	34.0
3	31.94281712665964	34.0	31.0	34.0	30.0	34.0
4	35.298899391593096	37.0	35.0	37.0	33.0	37.0
5	35.36454562627282	37.0	35.0	37.0	33.0	37.0
6	35.485644918746424	37.0	37.0	37.0	35.0	37.0
7	35.44768342677521	37.0	37.0	37.0	35.0	37.0
8	35.46262006564809	37.0	37.0	37.0	35.0	37.0
9	37.156037321566345	39.0	39.0	39.0	35.0	39.0
10-11	37.144746295488574	39.0	38.5	39.0	35.0	39.0
12-13	37.09513653869215	39.0	38.0	39.0	35.0	39.0
14-15	38.60792205111552	41.0	39.5	41.0	36.0	41.0
16-17	38.63054244044075	41.0	39.5	41.0	35.5	41.0
18-19	38.646517233950654	41.0	39.5	41.0	35.0	41.0
20-21	38.682399100283675	41.0	40.0	41.0	35.0	41.0
22-23	38.67460789963249	41.0	39.0	41.0	35.0	41.0
24-25	38.705876438108156	41.0	39.0	41.0	35.0	41.0
26-27	38.682078606851135	41.0	39.0	41.0	35.0	41.0
28-29	38.6654531426928	41.0	39.0	41.0	35.0	41.0
30-31	38.62716996339795	41.0	39.0	41.0	35.0	41.0
32-33	38.57773225957421	41.0	39.0	41.0	35.0	41.0
34-35	38.512383967050724	41.0	39.0	41.0	34.0	41.0
36-37	38.43010059461083	41.0	39.0	41.0	34.0	41.0
38-39	38.34497801648524	41.0	39.0	41.0	34.0	41.0
40-41	38.26673211842975	41.0	39.0	41.0	34.0	41.0
42-43	38.16305395220403	41.0	38.5	41.0	33.0	41.0
44-45	38.043807976678266	40.0	38.0	41.0	33.0	41.0
46-47	37.92853367887195	40.0	38.0	41.0	33.0	41.0
48-49	37.804313687722654	40.0	38.0	41.0	33.0	41.0
50-51	37.6734098962429	40.0	38.0	41.0	33.0	41.0
52-53	37.52284125917837	40.0	37.0	41.0	32.5	41.0
54-55	37.36441376763377	40.0	37.0	41.0	32.0	41.0
56-57	37.18527491862969	40.0	36.5	41.0	32.0	41.0
58-59	36.97888643389504	40.0	36.0	41.0	31.0	41.0
60-61	36.73982539475346	39.0	35.5	41.0	31.0	41.0
62-63	36.489919712150204	39.0	35.0	41.0	31.0	41.0
64-65	36.2143319728281	39.0	35.0	41.0	31.0	41.0
66-67	35.91687931193668	38.0	35.0	40.0	30.5	41.0
68-69	35.5987424877719	37.5	35.0	40.0	30.0	41.0
70-71	35.25574354476349	37.0	35.0	40.0	30.0	41.0
72-73	34.96079288164984	36.5	35.0	39.0	30.0	41.0
74-75	34.67564910532454	36.0	34.5	39.0	30.0	41.0
76-77	34.348800855781136	35.5	34.0	38.5	29.0	40.0
78-79	33.97970862689789	35.0	34.0	37.0	29.0	39.5
80-81	33.65667406348529	35.0	34.0	37.0	29.0	39.0
82-83	33.339181542549746	35.0	34.0	36.5	29.0	39.0
84-85	33.173472907161965	35.0	34.0	36.0	29.0	37.5
86-87	32.94619782828556	35.0	34.0	36.0	29.0	37.0
88-89	32.71933623369277	35.0	34.0	35.5	29.0	37.0
90-91	32.527985603265215	35.0	34.0	35.0	28.5	36.0
92-93	32.3436573076217	35.0	34.0	35.0	28.0	36.0
94-95	32.174980393655076	35.0	34.0	35.0	27.5	36.0
96-97	32.014394612314376	35.0	34.0	35.0	27.0	36.0
98-99	31.84445323502038	35.0	34.0	35.0	27.0	35.5
100	31.724507241347474	35.0	34.0	35.0	27.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.30898752583269484
1101	2	0.3619362247520179
1101	3	0.38601153182461445
1101	4	0.3508012404911156
1101	5	0.2286734834644264
1101	6	0.2894746364507341
1101	7	0.35232261207206506
1101	8	0.3276250409758319
1101	9	0.38116928363275804
1101	10-11	0.34071289620256806
1101	12-13	0.15176445654543613
1101	14-15	0.15816339250481093
1101	16-17	0.22373048348980973
1101	18-19	0.27427397570477297
1101	20-21	0.23568924608573383
1101	22-23	0.2086866071390645
1101	24-25	0.22977893038112285
1101	26-27	0.28984918968194506
1101	28-29	0.21646729248986674
1101	30-31	0.23701117048267406
1101	32-33	0.22135676998230025
1101	34-35	0.23298152247976844
1101	36-37	0.22210714644313612
1101	38-39	0.21922454468872843
1101	40-41	0.23681479955094176
1101	42-43	0.20826924987078144
1101	44-45	0.28475850367671285
1101	46-47	0.28139587893466
1101	48-49	0.24615495820270894
1101	50-51	0.24078256016534283
1101	52-53	0.28630252894475205
1101	54-55	0.29233834239486356
1101	56-57	0.29574951605666655
1101	58-59	0.2640882915849829
1101	60-61	0.3034928077286452
1101	62-63	0.2991190178643066
1101	64-65	0.27478665373200784
1101	66-67	0.2953410880412548
1101	68-69	0.2582997383167296
1101	70-71	0.2654130509214312
1101	72-73	0.21486977342635782
1101	74-75	0.2929691705075754
1101	76-77	0.28851931756653926
1101	78-79	0.3011826034325722
1101	80-81	0.3061641883017856
1101	82-83	0.2796603824319206
1101	84-85	0.2078211488277688
1101	86-87	0.1625292406362746
1101	88-89	0.15021953744184202
1101	90-91	0.1709398204385053
1101	92-93	0.19701013818898616
1101	94-95	0.2547108759913499
1101	96-97	0.2269506564203425
1101	98-99	0.2635804397712498
1101	100	0.25009355892946417
1102	1	0.2477064243260223
1102	2	0.2629882394814622
1102	3	0.3576044139377217
1102	4	0.3284240882365381
1102	5	0.24000176086802583
1102	6	0.2524722566715525
1102	7	0.3346411325364542
1102	8	0.30220181799938217
1102	9	0.3509320984560915
1102	10-11	0.32521262185180433
1102	12-13	0.2888088827170989
1102	14-15	0.36065267883530083
1102	16-17	0.36012050924615124
1102	18-19	0.34286073505484893
1102	20-21	0.3328916065904153
1102	22-23	0.348266601760848
1102	24-25	0.4027549134195567
1102	26-27	0.40083752083653224
1102	28-29	0.40313435034600786
1102	30-31	0.38848034604695414
1102	32-33	0.4569672665027795
1102	34-35	0.5023475390465109
1102	36-37	0.49945665377754267
1102	38-39	0.5015461739436873
1102	40-41	0.510433408460429
1102	42-43	0.5378648583932133
1102	44-45	0.5262554174248706
1102	46-47	0.45999601385616273
1102	48-49	0.5075284685091219
1102	50-51	0.5500070801292196
1102	52-53	0.4534459156922921
1102	54-55	0.45853413091627715
1102	56-57	0.4537221649172096
1102	58-59	0.4377749441481029
1102	60-61	0.5232907130255526
1102	62-63	0.5018772145792454
1102	64-65	0.528193888198544
1102	66-67	0.483577502237857
1102	68-69	0.5065952661660518
1102	70-71	0.4943694921023436
1102	72-73	0.453102806813213
1102	74-75	0.40144237351004364
1102	76-77	0.40734490975228255
1102	78-79	0.4539251007551215
1102	80-81	0.440749522271382
1102	82-83	0.41102337725423155
1102	84-85	0.4695744367623931
1102	86-87	0.42327854465578696
1102	88-89	0.4347537706664184
1102	90-91	0.4548765441152156
1102	92-93	0.43010134854996096
1102	94-95	0.44623604087382773
1102	96-97	0.45665981351840657
1102	98-99	0.45171026131112413
1102	100	0.4855539785851022
1103	1	0.10551674335027883
1103	2	0.2211397533393189
1103	3	0.19269479707969595
1103	4	0.16880941755260892
1103	5	0.12186452053354202
1103	6	0.06556610999035684
1103	7	0.10876666049616546
1103	8	0.1256858369179099
1103	9	0.16997325317840506
1103	10-11	0.14076851464318452
1103	12-13	0.1717150788774262
1103	14-15	0.2411091675938124
1103	16-17	0.28096818788279165
1103	18-19	0.20774868216406617
1103	20-21	0.2594239361510162
1103	22-23	0.21235347339281674
1103	24-25	0.21041352911708344
1103	26-27	0.24567838583438117
1103	28-29	0.27460405938607835
1103	30-31	0.23505530007133757
1103	32-33	0.2551168373995196
1103	34-35	0.2180920823503314
1103	36-37	0.21577331842532033
1103	38-39	0.21486478773747564
1103	40-41	0.24024039684504572
1103	42-43	0.2197486466907037
1103	44-45	0.190842854220179
1103	46-47	0.21932892123651015
1103	48-49	0.22563750724570752
1103	50-51	0.22383510348156221
1103	52-53	0.23186954743960797
1103	54-55	0.2966568458061971
1103	56-57	0.24285246655740167
1103	58-59	0.22274647177262352
1103	60-61	0.3059412657361449
1103	62-63	0.3164870074954962
1103	64-65	0.2621682679632755
1103	66-67	0.2651152081287549
1103	68-69	0.24550371875591281
1103	70-71	0.3000437003645473
1103	72-73	0.31600414029096413
1103	74-75	0.2419732552025664
1103	76-77	0.2144186006256632
1103	78-79	0.20996732516200467
1103	80-81	0.25714974706956895
1103	82-83	0.2549364896320512
1103	84-85	0.20106126437603677
1103	86-87	0.16996297126426896
1103	88-89	0.1905854070515005
1103	90-91	0.16202629407210623
1103	92-93	0.20824023014353088
1103	94-95	0.14420075516123632
1103	96-97	0.14908514641266635
1103	98-99	0.18300562126956166
1103	100	0.13473469916119996
1104	1	-0.03037740399134492
1104	2	0.04525385627630385
1104	3	0.03090226207000768
1104	4	-0.028222749894929677
1104	5	0.024064198873468
1104	6	6.504917665992593E-4
1104	7	0.031918914852397506
1104	8	0.03977898299535809
1104	9	-0.002384245936099205
1104	10-11	0.050603552266188956
1104	12-13	0.04510089314968724
1104	14-15	0.09768224951201887
1104	16-17	0.08218794766001736
1104	18-19	0.0409275987426625
1104	20-21	0.03491868289769684
1104	22-23	0.051994298305494624
1104	24-25	0.11088334864763993
1104	26-27	0.12132562955019921
1104	28-29	0.11949631981843112
1104	30-31	0.06988686408226386
1104	32-33	0.1080378031860576
1104	34-35	0.09027989641526091
1104	36-37	0.08687117421678892
1104	38-39	0.13791371563321064
1104	40-41	0.11018521140815096
1104	42-43	0.10293836206329843
1104	44-45	0.11273578921719718
1104	46-47	0.06527525195028971
1104	48-49	0.05188142032548626
1104	50-51	0.07315103587823302
1104	52-53	0.07631432384697945
1104	54-55	0.0704189260570729
1104	56-57	0.06584684254548989
1104	58-59	0.0613932676984561
1104	60-61	0.05504914136859185
1104	62-63	0.04652358301073889
1104	64-65	0.043051504923369066
1104	66-67	-0.0020951898139998093
1104	68-69	-0.058516528262899214
1104	70-71	-0.0526913821814432
1104	72-73	-0.006055978035021781
1104	74-75	0.05997311697800001
1104	76-77	0.04522460821073793
1104	78-79	0.026191505245570568
1104	80-81	0.03556094210731686
1104	82-83	0.065059369108873
1104	84-85	0.028006937083304706
1104	86-87	0.035355158264827935
1104	88-89	0.059013045052893176
1104	90-91	0.08147585794481671
1104	92-93	0.0350464724223869
1104	94-95	0.041401923734738943
1104	96-97	0.05819713278602734
1104	98-99	0.03786848018547673
1104	100	-0.007674756550787976
1105	1	-0.0605780226904038
1105	2	-0.0130812526028663
1105	3	-0.03624229612923813
1105	4	-0.007324597901011032
1105	5	0.023396076708721125
1105	6	0.04981178235018291
1105	7	0.029966398791572146
1105	8	-0.004795398440435861
1105	9	0.06469544865203858
1105	10-11	0.05879834807392115
1105	12-13	0.0960142092040357
1105	14-15	0.13532431565402447
1105	16-17	0.09373865994607655
1105	18-19	0.12079671853941676
1105	20-21	0.05156486336148447
1105	22-23	0.07333934844331225
1105	24-25	0.09004095778302457
1105	26-27	0.11535581742542433
1105	28-29	0.12203055189260681
1105	30-31	0.10821711156188485
1105	32-33	0.14143683171526078
1105	34-35	0.16580183946474136
1105	36-37	0.18201402249796672
1105	38-39	0.1467111968753514
1105	40-41	0.14653091557615028
1105	42-43	0.14973135544477856
1105	44-45	0.11476308048043649
1105	46-47	0.12058452731183422
1105	48-49	0.1283256133466466
1105	50-51	0.13852735270393168
1105	52-53	0.1416559163902278
1105	54-55	0.1327201211117952
1105	56-57	0.12545717541313195
1105	58-59	0.16753670123550535
1105	60-61	0.21145917648394885
1105	62-63	0.11454902545878554
1105	64-65	0.1447247770700386
1105	66-67	0.14397199863838495
1105	68-69	0.21152983371111134
1105	70-71	0.26994096116147404
1105	72-73	0.23033085586558855
1105	74-75	0.2318128701456672
1105	76-77	0.15831262853556893
1105	78-79	0.17122074106862328
1105	80-81	0.16199293106027568
1105	82-83	0.11740076342029937
1105	84-85	0.11135286780196196
1105	86-87	0.1451035454399019
1105	88-89	0.1205326066234278
1105	90-91	0.17252751149491274
1105	92-93	0.12034515519213329
1105	94-95	0.03596322456704115
1105	96-97	0.06049507117503339
1105	98-99	0.07915177504115789
1105	100	0.0431197889724082
1106	1	-0.02091435049335999
1106	2	0.04588509608011293
1106	3	-0.00929705528027469
1106	4	0.052415115781990096
1106	5	0.0179155338153123
1106	6	0.05042221591909879
1106	7	0.09158094252795479
1106	8	0.06243577332738681
1106	9	0.10891768533432611
1106	10-11	0.10743942067793455
1106	12-13	0.12538558791075616
1106	14-15	0.1807375689530204
1106	16-17	0.11808051348318571
1106	18-19	0.16479041017190355
1106	20-21	0.16578043577111146
1106	22-23	0.13618584939334966
1106	24-25	0.2089082577068737
1106	26-27	0.2242841082919682
1106	28-29	0.21431596276042342
1106	30-31	0.1704556398532162
1106	32-33	0.19738974791940933
1106	34-35	0.17665745767158825
1106	36-37	0.19912923745559397
1106	38-39	0.19270298779028394
1106	40-41	0.266200600064451
1106	42-43	0.23289011330003007
1106	44-45	0.2178474127408947
1106	46-47	0.18773963458259146
1106	48-49	0.21216505257105212
1106	50-51	0.18011427416152515
1106	52-53	0.13262428007926275
1106	54-55	0.1770932723130585
1106	56-57	0.16457947932621408
1106	58-59	0.07788443232104214
1106	60-61	0.15129826363839527
1106	62-63	0.14159321407367997
1106	64-65	0.15553418130093632
1106	66-67	0.1299075133310481
1106	68-69	0.15283760485643683
1106	70-71	0.15742792313140086
1106	72-73	0.1448233030445465
1106	74-75	0.12872882552406395
1106	76-77	0.09755329903324395
1106	78-79	0.15477607620048417
1106	80-81	0.15758048711309414
1106	82-83	0.15756761185341617
1106	84-85	0.14626976382331947
1106	86-87	0.15740199210060268
1106	88-89	0.1573322907064778
1106	90-91	0.2243781223567467
1106	92-93	0.17853721203878337
1106	94-95	0.18997973682704128
1106	96-97	0.22394565846327197
1106	98-99	0.2786112313219071
1106	100	0.26980809801261785
1107	1	0.052186424019943445
1107	2	0.06610783959285627
1107	3	0.08348492286599196
1107	4	0.12438990771878622
1107	5	0.08785262740539679
1107	6	0.06968953718961046
1107	7	0.11133262052884163
1107	8	0.09540195168911225
1107	9	0.0951133021501036
1107	10-11	0.09867240930917376
1107	12-13	0.0739711673466843
1107	14-15	0.1324408664239698
1107	16-17	0.14869936992652555
1107	18-19	0.11888186142088841
1107	20-21	0.16858349193534394
1107	22-23	0.1796902949041126
1107	24-25	0.15091162390887547
1107	26-27	0.22065050551987042
1107	28-29	0.18225856373689453
1107	30-31	0.18016830596408084
1107	32-33	0.1546420982086758
1107	34-35	0.200217255234584
1107	36-37	0.26189380225103065
1107	38-39	0.2408960188149578
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2216	62-63	-0.2658882423240385
2216	64-65	-0.303710974457573
2216	66-67	-0.2962467113804479
2216	68-69	-0.2785486698998909
2216	70-71	-0.2746039458202034
2216	72-73	-0.32324021291314864
2216	74-75	-0.3188730328374447
2216	76-77	-0.33639874881399834
2216	78-79	-0.2214275298974897
2216	80-81	-0.2782909820941768
2216	82-83	-0.2747047063500858
2216	84-85	-0.2911722381331501
2216	86-87	-0.267293815131076
2216	88-89	-0.2353598105120085
2216	90-91	-0.24913933948469236
2216	92-93	-0.24651707097563502
2216	94-95	-0.2562301063968704
2216	96-97	-0.28429095164786133
2216	98-99	-0.3015972345284119
2216	100	-0.3069959779958715
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	39897.0
3	6083.0
4	3950.0
5	3492.0
6	2898.0
7	3476.0
8	4043.0
9	4424.0
10	4581.0
11	4818.0
12	5423.0
13	6044.0
14	6832.0
15	7086.0
16	7078.0
17	7011.0
18	6816.0
19	6565.0
20	6549.0
21	6857.0
22	8047.0
23	11396.0
24	17802.0
25	23993.0
26	24390.0
27	21156.0
28	22948.0
29	28994.0
30	36046.0
31	42953.0
32	56323.0
33	76533.0
34	111527.0
35	176857.0
36	344332.0
37	753750.0
38	1266440.0
39	600543.0
40	1235.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.813517808284644	19.351868446101214	12.818566442710678	24.016047302903463
2	15.628553745307364	19.882839082562523	40.36143954514184	24.12716762698827
3	18.684320786188387	25.03816822269107	29.454503744070315	26.823007247050228
4	12.236614174482789	16.26651422089626	38.75901849847643	32.73785310614453
5	12.931671500447534	38.27802165735623	35.566298470900264	13.22400837129597
6	31.476774427376025	37.80806459535634	17.66903907503953	13.0461219022281
7	28.131706779564258	32.168910944455284	22.113696798800927	17.585685477179524
8	25.414650401128974	36.54236514087639	20.003664127001827	18.039320330992805
9	25.23297660436433	16.878078984498675	20.83080872314111	37.058135687995886
10-11	24.120391470219754	26.88875857995597	28.65292085909549	20.33792909072878
12-13	24.68054267453892	24.22126012496585	28.651738346481924	22.446458854013308
14-15	21.829458405789232	26.306260548116406	25.61879517195794	26.24548587413642
16-17	21.68205842753668	29.71120502314008	26.271204216005216	22.335532333318024
18-19	21.70810918160525	28.77157684401801	28.00709798317873	21.513215991198013
20-21	22.70793325362936	27.98749852977761	27.61121452449623	21.6933536920968
22-23	22.435674392195875	27.681540732039945	27.675141990335245	22.207642885428935
24-25	22.26228887952235	27.8094182384197	27.620854338901264	22.307438543156692
26-27	22.053381046534444	27.80381654277713	27.763658757861965	22.379143652826457
28-29	22.04777655000592	27.901553523743576	27.806983821200078	22.243686105050426
30-31	22.215288019223895	28.20616062561291	27.490176508876157	22.08837484628704
32-33	21.946112108818685	28.008101609701754	27.85294067669708	22.192845604782484
34-35	22.167553405087485	27.76983572495396	27.874322135945967	22.18828873401259
36-37	22.355034795047455	28.102363705680517	27.427834657417723	22.114766841854305
38-39	22.136032625996034	28.2247866558593	27.629722237783106	22.00945848036156
40-41	22.019502332752992	27.918735633938653	27.857325388676006	22.204436644632345
42-43	21.958812225566955	27.87880638427098	27.76573748021095	22.396643909951113
44-45	22.028610474682594	27.990042065793418	27.78996203909307	22.19138542043092
46-47	22.104469237993555	28.130161972936097	27.686549270961148	22.078819518109206
48-49	22.003001316909778	27.960229032636235	27.760043876635066	22.27672577381892
50-51	21.961846088810532	27.912111139095536	27.77295758199684	22.35308519009709
52-53	22.035531350488718	27.85605036683776	27.784886762241868	22.323531520431654
54-55	22.090306915737614	28.202187564765875	27.64460191315992	22.06290360633659
56-57	22.023370449262593	27.95845395080984	27.691807676927315	22.326367923000245
58-59	21.946623286464533	27.611584510740013	28.02761385477554	22.414178348019913
60-61	22.19045405104146	28.0039939742553	27.62180234128884	22.183749633414394
62-63	21.89917844243136	28.010888605014312	27.759875564868953	22.330057387685372
64-65	21.94739567892982	27.805609592866602	27.81253970376033	22.434455024443245
66-67	21.823545085267174	27.842769014799167	27.726019773556388	22.60766612637727
68-69	22.168914881417276	27.965869140877814	27.656685679146186	22.208530298558724
70-71	22.12876009846292	27.820699462680924	27.667936439324524	22.382603999531632
72-73	21.91809719100057	27.91454384143825	27.823433335399905	22.34392563216128
74-75	22.028406500667362	28.00605683902142	27.668286903851264	22.29724975645995
76-77	22.02442675815826	27.900811946301435	27.55664314813357	22.51811814740673
78-79	22.09809699831108	27.978688972477862	27.63267418294762	22.290539846263442
80-81	22.060771548713063	27.866118962825016	27.700968783695483	22.37214070476644
82-83	22.00961822484343	27.861216117994182	27.837054393749817	22.29211126341258
84-85	21.971215111634937	27.87880413130442	27.690383960030974	22.459596797029672
86-87	22.14722796726804	27.945801284208578	27.584172056920224	22.32279869160315
88-89	21.88826741330469	27.823917542360473	27.663897130266097	22.62391791406875
90-91	21.953430925495372	27.772096096871067	27.728833017256644	22.545639960376914
92-93	22.125450850193616	27.942609773808275	27.567605957262654	22.364333418735455
94-95	21.933114075116407	27.713393446812184	27.731606702531202	22.621885775540207
96-97	21.995165821765163	27.69513136019141	27.772550427265102	22.537152390778317
98-99	21.995743563507112	27.826188075977605	27.623667507316412	22.55440085319887
100	22.10192966628949	27.55937914669043	27.61006202479824	22.728629162221846
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3125.0
1	1859.0
2	541.5
3	458.5
4	372.5
5	293.5
6	272.0
7	269.5
8	268.0
9	266.5
10	272.0
11	271.0
12	268.0
13	297.5
14	352.5
15	435.5
16	589.0
17	762.5
18	1075.5
19	1575.0
20	2297.5
21	3434.5
22	4967.0
23	7452.0
24	10866.0
25	14974.0
26	20570.0
27	28307.0
28	38066.0
29	48266.0
30	57950.0
31	68302.0
32	78615.0
33	88661.0
34	98030.5
35	106285.0
36	114566.0
37	122054.5
38	127262.5
39	129210.0
40	131358.0
41	134496.5
42	134930.0
43	134009.0
44	131966.5
45	129533.0
46	129223.0
47	127730.0
48	126536.5
49	123751.5
50	119236.5
51	115732.5
52	108572.5
53	103000.5
54	100276.5
55	95373.0
56	88093.0
57	79564.5
58	72078.5
59	67103.0
60	60799.5
61	52280.5
62	45431.0
63	39247.5
64	32072.0
65	25562.0
66	20457.0
67	15660.5
68	11493.5
69	8395.0
70	5981.0
71	4395.5
72	3572.5
73	2631.0
74	1906.0
75	1419.5
76	921.5
77	626.0
78	459.0
79	303.5
80	190.0
81	124.0
82	94.5
83	74.5
84	62.5
85	59.5
86	52.0
87	35.5
88	22.0
89	17.5
90	20.5
91	16.5
92	10.5
93	8.0
94	6.5
95	3.5
96	2.0
97	3.0
98	4.5
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07489676821639037
2	0.07733761223902867
3	0.07869068881679556
4	0.08065397640022201
5	0.07919477616929695
6	0.07887640520982238
7	0.07808047781113599
8	0.07808047781113599
9	0.07773557593837188
10-11	0.07758965591527936
12-13	0.076382499360605
14-15	0.07472431728000832
16-17	0.07372940803165032
18-19	0.07338450615888621
20-21	0.07298654245954302
22-23	0.0748835027597456
24-25	0.07496309549961425
26-27	0.07415390264428307
28-29	0.07041304387045698
30-31	0.07517534280593062
32-33	0.07364981529178168
34-35	0.07082427302644495
36-37	0.06791913802123958
38-39	0.06953752373190195
40-41	0.0720446950377641
42-43	0.07695291399633025
44-45	0.07813353963771506
46-47	0.08131724923246068
48-49	0.08422238423766604
50-51	0.08501831163635244
52-53	0.08537647896576132
54-55	0.08541627533569565
56-57	0.08357237686207214
58-59	0.08244481304726642
60-61	0.07997743811133858
62-63	0.08089275461982792
64-65	0.07997743811133858
66-67	0.07949988167212674
68-69	0.07899579431962533
70-71	0.07686005579981683
72-73	0.0764753575571184
74-75	0.07606412840113043
76-77	0.0760773938577752
78-79	0.0762763757074468
80-81	0.07744373589218685
82-83	0.07695291399633025
84-85	0.07618351751093339
86-87	0.07692638308304069
88-89	0.07411410627434875
90-91	0.07155387314190749
92-93	0.0714477494887493
94-95	0.07164673133842091
96-97	0.07094366213624792
98-99	0.07049263661032562
100	0.06977630195150786
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3769188.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.18656870412915
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.54210924489698	44.11891670173795
2	14.728803442169976	17.434946737192146
3	4.907829442106802	8.714327533902063
4	2.098418998047217	4.967928807918858
5	1.087329229695867	3.217764307870113
6	0.6392550124478689	2.270118642822284
7	0.4229825676610422	1.7524420761063462
8	0.274272477462232	1.2986597464776086
9	0.20264969141606712	1.0794725895480866
>10	1.0177411944625787	10.655709425993347
>50	0.05780582544746672	2.3085759275075555
>100	0.020220235162815788	1.8728734578577064
>500	4.930022541759544E-4	0.1969855290575689
>1k	8.96367688425404E-5	0.1112785160083805
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	2.6530913289546713E-5	0.0	0.0	0.0	0.0
9	2.6530913289546713E-5	0.0	0.0	0.0	0.0
10-11	2.6530913289546713E-5	0.0	0.0	0.0	0.0
12-13	2.6530913289546713E-5	0.0	0.0	0.0	0.0
14-15	2.6530913289546713E-5	0.0	0.0	0.0	0.0
16-17	2.6530913289546713E-5	0.0	0.0	0.0	0.0
18-19	2.6530913289546713E-5	0.0	0.0	3.979636993432007E-5	0.0
20-21	2.6530913289546713E-5	0.0	0.0	7.959273986864014E-5	0.0
22-23	2.6530913289546713E-5	0.0	0.0	1.4592002309250692E-4	0.0
24-25	2.6530913289546713E-5	0.0	0.0	2.785745895402405E-4	0.0
26-27	2.6530913289546713E-5	0.0	0.0	4.3776006927752077E-4	0.0
28-29	2.6530913289546713E-5	0.0	0.0	0.0011673601847400554	0.0
30-31	2.6530913289546713E-5	0.0	0.0	0.004072495189945421	0.0
32-33	2.6530913289546713E-5	0.0	0.0	0.011514416367663274	0.0
34-35	2.6530913289546713E-5	0.0	0.0	0.022511479926180387	0.0
36-37	2.6530913289546713E-5	0.0	0.0	0.04284742496261794	0.0
38-39	2.6530913289546713E-5	0.0	0.0	0.08162235473529046	0.0
40-41	2.6530913289546713E-5	0.0	0.0	0.12787900205561517	0.0
42-43	2.6530913289546713E-5	0.0	0.0	0.17617852969923495	0.0
44-45	2.6530913289546713E-5	0.0	0.0	0.23495776809222568	0.0
46-47	2.6530913289546713E-5	0.0	0.0	0.3058085720319602	0.0
48-49	2.6530913289546713E-5	0.0	0.0	0.38625030112586584	0.0
50-51	2.6530913289546713E-5	0.0	0.0	0.4742002786807132	0.0
52-53	2.6530913289546713E-5	0.0	0.0	0.565519682223333	0.0
54-55	2.6530913289546713E-5	0.0	0.0	0.6634718140883394	0.0
56-57	2.6530913289546713E-5	0.0	0.0	0.7802741598455689	0.0
58-59	2.6530913289546713E-5	0.0	0.0	0.916404275934233	0.0
60-61	2.6530913289546713E-5	0.0	0.0	1.0670202706789897	0.0
62-63	2.6530913289546713E-5	0.0	0.0	1.2195730220938832	0.0
64-65	2.6530913289546713E-5	0.0	0.0	1.37838706904511	0.0
66-67	2.6530913289546713E-5	0.0	0.0	1.5599779050554123	0.0
68-69	2.6530913289546713E-5	0.0	0.0	1.7637618500324206	0.0
70-71	2.6530913289546713E-5	0.0	0.0	1.9867011144044817	0.0
72-73	2.6530913289546713E-5	0.0	0.0	2.2190588529943316	0.0
74-75	2.6530913289546713E-5	0.0	0.0	2.4530747736647784	0.0
76-77	2.6530913289546713E-5	0.0	0.0	2.7006081946562497	0.0
78-79	2.6530913289546713E-5	0.0	0.0	2.985749185235653	0.0
80-81	2.6530913289546713E-5	0.0	0.0	3.302727271762512	0.0
82-83	2.6530913289546713E-5	0.0	0.0	3.6345626697315176	0.0
84-85	2.6530913289546713E-5	0.0	0.0	3.954406094893648	0.0
86-87	2.6530913289546713E-5	0.0	0.0	4.288562947775489	0.0
88	2.6530913289546713E-5	0.0	0.0	4.566076300784148	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	11425	0.0	28.385633	1
TACATGG	11620	0.0	26.37225	2
ACATGGG	11735	0.0	24.792097	3
GTATCAA	7215	0.0	23.451567	1
TCAACGC	7170	0.0	22.222158	4
CAACGCA	7385	0.0	21.702204	5
ATCAACG	7410	0.0	21.565842	3
AACGCAG	7615	0.0	21.10844	6
GGTATCA	5495	0.0	20.955791	1
CATGGGG	7755	0.0	19.697325	4
GAGTACA	8300	0.0	19.593126	1
TATCAAC	8470	0.0	19.477331	2
ACGCAGA	8710	0.0	18.238653	7
TAGACCG	155	0.0010338863	18.193634	5
AGTACAT	8300	0.0	18.177439	2
CGCAGAG	8860	0.0	17.876823	8
GTCGTAC	285	9.598298E-7	16.491528	1
CATGGGA	4365	0.0	16.36685	4
TACACTG	2935	0.0	16.33398	5
GTATAGG	1535	0.0	15.615917	1
>>END_MODULE