FastQCFastQC Report
Wed 25 May 2016
SRR1032938_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032938_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3769188
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68640.18210818881944865No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55720.14783024884935428No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA121600.034.8118481
GGTATCA77200.034.3239821
TCAACGC136650.030.5349794
ATCAACG137150.030.4236583
CAACGCA139000.030.048995
AACGCAG143350.029.1695676
TATCAAC145300.028.8139132
GTACATG117050.027.294391
TACATGG117900.026.014272
ACATGGG117050.025.6408793
ACGCAGA165350.025.2875187
CGCAGAG167000.024.9528488
GCAGAGT182150.022.5139569
GAGTACT105400.021.48163812-13
CATGGGG79850.020.972914
AGAGTAC149150.020.29834410-11
GAGTACA87300.020.1276591
GTACTTT116500.019.25344514-15
GTCGTAC3004.26553E-918.792991
AGTACTT114300.018.432212-13
Produced by FastQC (version 0.11.5)