##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032938_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3769188 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.86048453937559 34.0 31.0 34.0 31.0 34.0 2 32.919691986709076 34.0 31.0 34.0 31.0 34.0 3 33.00154967064524 34.0 33.0 34.0 31.0 34.0 4 36.39378454988183 37.0 37.0 37.0 35.0 37.0 5 36.375661548322874 37.0 37.0 37.0 35.0 37.0 6 36.43302934212886 37.0 37.0 37.0 35.0 37.0 7 36.398460623349116 37.0 37.0 37.0 35.0 37.0 8 36.3401997990018 37.0 37.0 37.0 35.0 37.0 9 38.187651557842166 39.0 39.0 39.0 37.0 39.0 10-11 38.202366000316246 39.0 39.0 39.0 37.0 39.0 12-13 38.19218250721376 39.0 39.0 39.0 37.0 39.0 14-15 39.75934737137017 41.0 40.0 41.0 37.0 41.0 16-17 39.77275437043735 41.0 40.0 41.0 37.0 41.0 18-19 39.79387470192519 41.0 40.0 41.0 37.5 41.0 20-21 39.8043902294075 41.0 40.0 41.0 37.5 41.0 22-23 39.76779972238052 41.0 40.0 41.0 37.0 41.0 24-25 39.749385676702786 41.0 40.0 41.0 37.0 41.0 26-27 39.67942962781373 41.0 40.0 41.0 37.0 41.0 28-29 39.620612715523876 41.0 40.0 41.0 37.0 41.0 30-31 39.517418871120256 41.0 40.0 41.0 36.5 41.0 32-33 39.402665242487245 41.0 39.5 41.0 36.0 41.0 34-35 39.316391222724896 41.0 39.0 41.0 35.5 41.0 36-37 39.230424828902144 41.0 39.0 41.0 35.0 41.0 38-39 39.138193690524325 41.0 39.0 41.0 35.0 41.0 40-41 39.04175541257162 41.0 39.0 41.0 35.0 41.0 42-43 38.91108403189228 41.0 39.0 41.0 35.0 41.0 44-45 38.74804759009102 41.0 39.0 41.0 35.0 41.0 46-47 38.53535164603092 41.0 39.0 41.0 35.0 41.0 48-49 38.34388282038466 41.0 38.0 41.0 35.0 41.0 50-51 38.19211538400313 41.0 38.0 41.0 34.0 41.0 52-53 38.05094147598899 40.0 38.0 41.0 34.0 41.0 54-55 37.90378988790158 40.0 37.5 41.0 34.0 41.0 56-57 37.695933845698335 40.0 37.0 41.0 33.5 41.0 58-59 37.52831869888156 40.0 37.0 41.0 33.0 41.0 60-61 37.33364135723663 40.0 36.0 41.0 33.0 41.0 62-63 37.09682085372234 39.0 36.0 41.0 33.0 41.0 64-65 36.810415399815554 39.0 35.0 41.0 32.5 41.0 66-67 36.536067052107775 39.0 35.0 41.0 32.0 41.0 68-69 36.24151169960214 37.5 35.0 40.5 32.0 41.0 70-71 35.91835734911604 37.0 35.0 40.0 32.0 41.0 72-73 35.57589671833827 37.0 35.0 39.0 31.0 41.0 74-75 35.22837160152267 36.0 35.0 39.0 31.0 41.0 76-77 34.880792759607644 36.0 35.0 39.0 31.0 40.5 78-79 34.53783825587899 35.0 35.0 37.0 31.0 39.5 80-81 34.205893815856356 35.0 35.0 37.0 31.0 39.0 82-83 33.89714614925018 35.0 34.0 36.5 30.0 39.0 84-85 33.62153585865178 35.0 34.0 36.0 30.0 37.5 86-87 33.47511612580747 35.0 34.0 36.0 30.0 37.0 88-89 33.28525427757915 35.0 34.0 35.5 30.0 37.0 90-91 33.11435380776973 35.0 34.0 35.0 30.0 36.0 92-93 32.95436563525088 35.0 34.0 35.0 30.0 36.0 94-95 32.85891337869059 35.0 34.0 35.0 30.0 36.0 96-97 32.80009766029182 35.0 34.0 35.0 30.0 36.0 98-99 32.69291436245685 35.0 34.0 35.0 30.0 36.0 100 32.61238627524018 35.0 34.0 35.0 30.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23599641122811477 1101 2 0.234663430658685 1101 3 0.23814380508875388 1101 4 0.1583252095323857 1101 5 0.1495495318792237 1101 6 0.16247011500924913 1101 7 0.13816762753069156 1101 8 0.029476262487982297 1101 9 0.12095051616852004 1101 10-11 0.18093331715257222 1101 12-13 0.23650864393707138 1101 14-15 0.3299103106528989 1101 16-17 0.32074509834099274 1101 18-19 0.3172968540157939 1101 20-21 0.3195681661322496 1101 22-23 0.2877443122341319 1101 24-25 0.30985944418385003 1101 26-27 0.3405271028521355 1101 28-29 0.31324737407170744 1101 30-31 0.3689965072407233 1101 32-33 0.42167086663820186 1101 34-35 0.3418854966305531 1101 36-37 0.34527179684714326 1101 38-39 0.3499637931377322 1101 40-41 0.40025331039854706 1101 42-43 0.38341490179738713 1101 44-45 0.39435925894689916 1101 46-47 0.3859191031846194 1101 48-49 0.35106402456000296 1101 50-51 0.3239113617887597 1101 52-53 0.34748830004724596 1101 54-55 0.36040228795128115 1101 56-57 0.3230478210262646 1101 58-59 0.33718221886486077 1101 60-61 0.3684885168021381 1101 62-63 0.38427683514246525 1101 64-65 0.4294526936605507 1101 66-67 0.45828010237246275 1101 68-69 0.4658427012673556 1101 70-71 0.48999271633277885 1101 72-73 0.44871181954335526 1101 74-75 0.48139653090623113 1101 76-77 0.4754883810348147 1101 78-79 0.4720688470810117 1101 80-81 0.42224355919267964 1101 82-83 0.3903310251030163 1101 84-85 0.4272885134694633 1101 86-87 0.40317967972091395 1101 88-89 0.3588420822797431 1101 90-91 0.4016757249771956 1101 92-93 0.39906003262588285 1101 94-95 0.3690724139522885 1101 96-97 0.3688660947064406 1101 98-99 0.3848696870762254 1101 100 0.3783236040888127 1102 1 0.19587055184182134 1102 2 0.21754712924899877 1102 3 0.20142891071768787 1102 4 0.12487205822910141 1102 5 0.12712271669526842 1102 6 0.15448075639937286 1102 7 0.14662260524843873 1102 8 0.046258291191442424 1102 9 0.1222558973295591 1102 10-11 0.15608117900413276 1102 12-13 0.14773172763970166 1102 14-15 0.23295420522137533 1102 16-17 0.2541186646056417 1102 18-19 0.27239457107543785 1102 20-21 0.26497527780156105 1102 22-23 0.2537214361987097 1102 24-25 0.2299849382872523 1102 26-27 0.2468097742980575 1102 28-29 0.23375334528120106 1102 30-31 0.20848564745805476 1102 32-33 0.19162638761709871 1102 34-35 0.23483596427037412 1102 36-37 0.2245934432004475 1102 38-39 0.21600161623822345 1102 40-41 0.191244346200925 1102 42-43 0.1852288422360644 1102 44-45 0.23212927683799478 1102 46-47 0.19806386557198152 1102 48-49 0.23921378121942638 1102 50-51 0.25414520501556126 1102 52-53 0.23626719819861108 1102 54-55 0.20617678094640013 1102 56-57 0.16520620768830696 1102 58-59 0.20386950717564645 1102 60-61 0.23658972192480832 1102 62-63 0.19374590034468753 1102 64-65 0.21027017345730314 1102 66-67 0.2159667036405324 1102 68-69 0.23071317350818532 1102 70-71 0.2108769884949595 1102 72-73 0.23941354352317035 1102 74-75 0.26013667798311957 1102 76-77 0.23030611264359635 1102 78-79 0.24775573435781695 1102 80-81 0.21108552693276295 1102 82-83 0.2640127036012174 1102 84-85 0.2560056166362301 1102 86-87 0.23522027430819747 1102 88-89 0.23938723202240908 1102 90-91 0.2650136800458398 1102 92-93 0.2533277803226568 1102 94-95 0.27081022788184583 1102 96-97 0.23594746108206976 1102 98-99 0.24181973813811197 1102 100 0.23071892722427378 1103 1 0.12148421755626515 1103 2 0.1598195746360318 1103 3 0.18222191792821718 1103 4 0.03375340439945518 1103 5 0.08117854997593099 1103 6 0.10491914454416928 1103 7 0.06983626897164896 1103 8 0.03387817438147067 1103 9 0.07105525144584135 1103 10-11 0.08046182620628883 1103 12-13 0.09912871743779306 1103 14-15 0.1339696513097124 1103 16-17 0.1374239371353525 1103 18-19 0.14875126856649956 1103 20-21 0.13029784555140367 1103 22-23 0.14771286288277707 1103 24-25 0.12368929548315322 1103 26-27 0.17753998600086618 1103 28-29 0.1879657242062862 1103 30-31 0.1783372983037239 1103 32-33 0.19538915885288333 1103 34-35 0.16361416379407956 1103 36-37 0.16245534422196783 1103 38-39 0.14060173281588817 1103 40-41 0.15832789971388905 1103 42-43 0.16869517462694006 1103 44-45 0.14877733854571318 1103 46-47 0.18406215688249006 1103 48-49 0.19499127717397613 1103 50-51 0.13807277570924725 1103 52-53 0.22235628296586896 1103 54-55 0.2020733881108896 1103 56-57 0.2325850218997445 1103 58-59 0.20051054525995227 1103 60-61 0.25609285411992744 1103 62-63 0.2542986682572277 1103 64-65 0.2506488238445286 1103 66-67 0.2479383136297102 1103 68-69 0.20912761842848226 1103 70-71 0.20063377064361276 1103 72-73 0.13389278468823562 1103 74-75 0.18906963179539815 1103 76-77 0.20331631982691079 1103 78-79 0.20548367741216111 1103 80-81 0.21708149664610232 1103 82-83 0.20034629524177916 1103 84-85 0.16582248425405055 1103 86-87 0.20368381293820192 1103 88-89 0.15758025329225234 1103 90-91 0.1928917703986599 1103 92-93 0.24981287616660808 1103 94-95 0.28336280511869916 1103 96-97 0.23246131290142102 1103 98-99 0.23701251216314034 1103 100 0.25213452977529016 1104 1 0.11125308016968916 1104 2 0.13337674777093156 1104 3 0.13016258791419233 1104 4 0.07852156869086713 1104 5 0.1007627748097022 1104 6 0.08074442437691687 1104 7 0.10339751685148713 1104 8 0.06112925498078425 1104 9 0.0741522015784497 1104 10-11 0.07440049913530089 1104 12-13 0.12240483396110591 1104 14-15 0.18921251978615317 1104 16-17 0.1907228067176021 1104 18-19 0.16857760236154462 1104 20-21 0.13546958597142833 1104 22-23 0.16736045863928695 1104 24-25 0.14137946584492767 1104 26-27 0.16154391244236166 1104 28-29 0.1289251358591912 1104 30-31 0.18661783153377343 1104 32-33 0.1820546460512702 1104 34-35 0.18345399608161017 1104 36-37 0.16546259237249217 1104 38-39 0.16482481358222856 1104 40-41 0.1947937172113967 1104 42-43 0.2056542270399646 1104 44-45 0.15702709521288227 1104 46-47 0.16183840083157008 1104 48-49 0.12337066976341049 1104 50-51 0.1222847409169745 1104 52-53 0.13337132280621944 1104 54-55 0.10945461577538396 1104 56-57 0.11417104437274617 1104 58-59 0.06417548315968702 1104 60-61 0.12423500800418452 1104 62-63 0.07212270766850537 1104 64-65 0.06803203848711803 1104 66-67 0.06553915996718729 1104 68-69 0.07787090517822293 1104 70-71 0.08023373291982949 1104 72-73 0.07455767723303808 1104 74-75 0.09264786617818999 1104 76-77 0.14923378176476376 1104 78-79 0.21553825451699282 1104 80-81 0.15953121386168334 1104 82-83 0.16529263603444377 1104 84-85 0.15581270414629955 1104 86-87 0.11352655370091469 1104 88-89 0.10180408637457816 1104 90-91 0.06554938599614957 1104 92-93 0.06910765732544633 1104 94-95 0.10230288189092107 1104 96-97 0.0852951324204625 1104 98-99 0.07218015264104594 1104 100 0.05343636038684707 1105 1 0.04293389726240093 1105 2 0.09726383114696802 1105 3 0.09178423213256792 1105 4 0.0384914833028418 1105 5 0.02070388512368737 1105 6 0.03055772236115928 1105 7 0.03969571954959861 1105 8 -0.01865777391520851 1105 9 0.051720719667443404 1105 10-11 0.0799942101969009 1105 12-13 0.08565245113364028 1105 14-15 0.09113095210953759 1105 16-17 0.13275108913996547 1105 18-19 0.1051854242927206 1105 20-21 0.1086651706609203 1105 22-23 0.14579988195998084 1105 24-25 0.11407495344128193 1105 26-27 0.08125092040938853 1105 28-29 0.14307211194730485 1105 30-31 0.14344479402884502 1105 32-33 0.172873984037885 1105 34-35 0.1904803029282931 1105 36-37 0.17816992668035425 1105 38-39 0.1964484198911265 1105 40-41 0.19402691740126699 1105 42-43 0.16724883351607644 1105 44-45 0.13860168297159703 1105 46-47 0.22146434276987037 1105 48-49 0.2648132243378498 1105 50-51 0.2750074955032815 1105 52-53 0.25361069110746826 1105 54-55 0.2720059942282589 1105 56-57 0.26862333792846016 1105 58-59 0.24021289638269394 1105 60-61 0.31261343121160223 1105 62-63 0.22939043256048564 1105 64-65 0.21425396913025452 1105 66-67 0.26023929050352024 1105 68-69 0.2506591369503326 1105 70-71 0.19037585300246462 1105 72-73 0.20330274166512652 1105 74-75 0.2323765919925549 1105 76-77 0.21064010171902225 1105 78-79 0.20097664352604028 1105 80-81 0.20838507655040672 1105 82-83 0.19267920562594298 1105 84-85 0.17551347522764615 1105 86-87 0.17262337174297926 1105 88-89 0.16290527091087625 1105 90-91 0.1417305172930412 1105 92-93 0.21963775889565795 1105 94-95 0.136637173569504 1105 96-97 0.16154116104135596 1105 98-99 0.14383300151558132 1105 100 0.16171476417563468 1106 1 0.041026084620376935 1106 2 0.07288496807725409 1106 3 0.02017085045715561 1106 4 0.030567735979644794 1106 5 0.04327982785364526 1106 6 0.04160260693424078 1106 7 0.03502503983085603 1106 8 0.07400674496278015 1106 9 0.0376040438879528 1106 10-11 0.03913296960924839 1106 12-13 0.0451540829408259 1106 14-15 0.06708728418489329 1106 16-17 0.043610242108741204 1106 18-19 0.06424009727994218 1106 20-21 0.029402193450479785 1106 22-23 0.05684977144913006 1106 24-25 0.11821883857800941 1106 26-27 0.10211860794257177 1106 28-29 0.030042921972835757 1106 30-31 0.09510459166660468 1106 32-33 0.1464010142280756 1106 34-35 0.05588666823551591 1106 36-37 0.10743103104225327 1106 38-39 0.11534349933634047 1106 40-41 0.13962721905132724 1106 42-43 0.09939904645192854 1106 44-45 0.10273324565980602 1106 46-47 0.13765063124300525 1106 48-49 0.16203973379943903 1106 50-51 0.18715622933621745 1106 52-53 0.14413470390215366 1106 54-55 0.1426103132343215 1106 56-57 0.15283278299128966 1106 58-59 0.05215995063483092 1106 60-61 0.05539344028564841 1106 62-63 0.06696102712533758 1106 64-65 0.072877043212344 1106 66-67 0.08316510435209068 1106 68-69 0.08267552111515641 1106 70-71 0.14083348002793628 1106 72-73 0.12896398184370383 1106 74-75 0.0848211195610773 1106 76-77 0.11245058376466943 1106 78-79 0.08196718608834885 1106 80-81 0.1160649253009467 1106 82-83 0.13945988125509956 1106 84-85 0.0807037138132074 1106 86-87 0.11705806487514536 1106 88-89 0.12528495317604893 1106 90-91 0.09243442709768601 1106 92-93 0.09013424529117486 1106 94-95 0.04206656788261398 1106 96-97 0.050652771181027845 1106 98-99 0.038647691142386975 1106 100 0.05849485813510569 1107 1 0.0375163374589178 1107 2 0.05234719177600766 1107 3 -0.011189068692672777 1107 4 0.015121794579947334 1107 5 0.027037835194597903 1107 6 0.018394408465567835 1107 7 0.03643242532206159 1107 8 0.025170048044479643 1107 9 0.013148325467916777 1107 10-11 0.03225516585761312 1107 12-13 0.05856136778969301 1107 14-15 0.10015858681209266 1107 16-17 0.07899010575196286 1107 18-19 0.05886287209813901 1107 20-21 0.06993054967788481 1107 22-23 0.08584459553954815 1107 24-25 0.06957820258763547 1107 26-27 0.10165950452737604 1107 28-29 0.12750266719934444 1107 30-31 0.12956952491934004 1107 32-33 0.10681098711769721 1107 34-35 0.12220205933054729 1107 36-37 0.1503241114113507 1107 38-39 0.1546963554708256 1107 40-41 0.20405507165901327 1107 42-43 0.16747447306603647 1107 44-45 0.1542511831792055 1107 46-47 0.1551764010611123 1107 48-49 0.12821284085482176 1107 50-51 0.16146374757887827 1107 52-53 0.11745823923769194 1107 54-55 0.1502866101203324 1107 56-57 0.1830999167010745 1107 58-59 0.19689288183565168 1107 60-61 0.18110564868403856 1107 62-63 0.1556827234755218 1107 64-65 0.11780621461799967 1107 66-67 0.09574354263268248 1107 68-69 0.1187115073056404 1107 70-71 0.11872962726842218 1107 72-73 0.08689161876272777 1107 74-75 0.10065052806970698 1107 76-77 0.11288764848498545 1107 78-79 0.10597585591927583 1107 80-81 0.06846038615818628 1107 82-83 0.07956958882727605 1107 84-85 0.12851908877331653 1107 86-87 0.058223898852126865 1107 88-89 0.11142765239627295 1107 90-91 0.08509912724099422 1107 92-93 0.10990919837934854 1107 94-95 0.16168666900220785 1107 96-97 0.15000231131104869 1107 98-99 0.13764207754988433 1107 100 0.18429747772918148 1108 1 0.03947122790965807 1108 2 0.027075175710045585 1108 3 0.04782711349598401 1108 4 -0.010047524765958826 1108 5 -0.01614688410831633 1108 6 -0.003157261727423588 1108 7 0.013165779785573761 1108 8 0.037935699458756744 1108 9 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-0.20558194069553792 2214 36-37 -0.1533389760717725 2214 38-39 -0.11649514208631473 2214 40-41 -0.1255191687799524 2214 42-43 -0.11874328958995051 2214 44-45 -0.11511704602794737 2214 46-47 -0.08215386137530345 2214 48-49 -0.050376893578473414 2214 50-51 -0.016133413196598667 2214 52-53 -0.05367023175919883 2214 54-55 -0.025123527962740866 2214 56-57 -0.08437086277822203 2214 58-59 -0.03660963728083999 2214 60-61 -0.05616713041596455 2214 62-63 -0.04139644985414037 2214 64-65 -0.06983143903948275 2214 66-67 -0.08696369215797972 2214 68-69 -0.05228330047123109 2214 70-71 -0.05844697952756661 2214 72-73 -0.042687602162935434 2214 74-75 -0.08612808366932967 2214 76-77 -0.03121195262377796 2214 78-79 -0.06384677564367536 2214 80-81 -0.12648262119115827 2214 82-83 -0.11616206570513299 2214 84-85 -0.12365454918979424 2214 86-87 -0.0669754652443828 2214 88-89 -0.05757856327216615 2214 90-91 -0.02518786028822717 2214 92-93 -0.025563247853746418 2214 94-95 -0.05291650717039431 2214 96-97 -0.04640197188647477 2214 98-99 -0.04783884655496706 2214 100 -0.07345569902719262 2215 1 -0.19115458831636545 2215 2 -0.18402572116818305 2215 3 -0.2046494427353096 2215 4 -0.11375437612083061 2215 5 -0.13631722401180468 2215 6 -0.1465157417466969 2215 7 -0.11146852252321793 2215 8 -0.06529303661722707 2215 9 -0.1098885174757882 2215 10-11 -0.16132510773311992 2215 12-13 -0.17980240917691503 2215 14-15 -0.24333008321725202 2215 16-17 -0.224203589412582 2215 18-19 -0.19089083807991614 2215 20-21 -0.2235160686258908 2215 22-23 -0.21247401517529596 2215 24-25 -0.2299287603511928 2215 26-27 -0.25352438003297095 2215 28-29 -0.2550915905322455 2215 30-31 -0.28831512813781757 2215 32-33 -0.2635293809187118 2215 34-35 -0.20585577906663133 2215 36-37 -0.20772935111230595 2215 38-39 -0.2459380439752863 2215 40-41 -0.23587151107762594 2215 42-43 -0.19799415809969645 2215 44-45 -0.17558190704151855 2215 46-47 -0.13129733789692466 2215 48-49 -0.15681410511203353 2215 50-51 -0.1456746606058914 2215 52-53 -0.12676325161659463 2215 54-55 -0.13670647633679778 2215 56-57 -0.1721966356145046 2215 58-59 -0.077895889760768 2215 60-61 -0.09419858619764199 2215 62-63 -0.0899672892324972 2215 64-65 -0.13091760392808993 2215 66-67 -0.1388402170910794 2215 68-69 -0.1837483908696882 2215 70-71 -0.20080513437552128 2215 72-73 -0.21970477549091072 2215 74-75 -0.27338524778846107 2215 76-77 -0.24903469544997847 2215 78-79 -0.2220935073492143 2215 80-81 -0.23595250495467468 2215 82-83 -0.2662051199218496 2215 84-85 -0.263480383893544 2215 86-87 -0.32815275264788113 2215 88-89 -0.33647071704653797 2215 90-91 -0.31093339875678794 2215 92-93 -0.30404112937419114 2215 94-95 -0.3441365469336617 2215 96-97 -0.29545688683696625 2215 98-99 -0.3395060064159807 2215 100 -0.35337635553347724 2216 1 -0.1936803519535033 2216 2 -0.2348185679548891 2216 3 -0.19098921870361352 2216 4 -0.1105453754118173 2216 5 -0.14170351157888916 2216 6 -0.1368208964001809 2216 7 -0.14304843238002718 2216 8 -0.09417587049421172 2216 9 -0.15995073549855476 2216 10-11 -0.20372676057792916 2216 12-13 -0.23606761037527946 2216 14-15 -0.3219633930593915 2216 16-17 -0.30031920534678136 2216 18-19 -0.27709262989244365 2216 20-21 -0.2711967514539353 2216 22-23 -0.2816093872233836 2216 24-25 -0.2910727801921311 2216 26-27 -0.3219384353328749 2216 28-29 -0.2811553990103448 2216 30-31 -0.2965071147570981 2216 32-33 -0.3942979462393481 2216 34-35 -0.4336700452759885 2216 36-37 -0.37447939129154406 2216 38-39 -0.33863639899691833 2216 40-41 -0.2789759901079236 2216 42-43 -0.330463588250943 2216 44-45 -0.3160229360170064 2216 46-47 -0.36988329629297567 2216 48-49 -0.4107871685202511 2216 50-51 -0.3505143769957755 2216 52-53 -0.3641359580553001 2216 54-55 -0.38768394538595174 2216 56-57 -0.38203181211437 2216 58-59 -0.375622193252525 2216 60-61 -0.42828232563376645 2216 62-63 -0.3966411257064024 2216 64-65 -0.4331536232197948 2216 66-67 -0.46559581080456525 2216 68-69 -0.4695935786796994 2216 70-71 -0.4555975114341706 2216 72-73 -0.45343157622582453 2216 74-75 -0.47335434873377125 2216 76-77 -0.4302074258679056 2216 78-79 -0.453323106770938 2216 80-81 -0.5067249273183876 2216 82-83 -0.4533859177281343 2216 84-85 -0.47416800510760027 2216 86-87 -0.45042094654536413 2216 88-89 -0.421958481560921 2216 90-91 -0.4262846129810214 2216 92-93 -0.4483147971763728 2216 94-95 -0.4799913414174739 2216 96-97 -0.4635041411177667 2216 98-99 -0.5224978755580807 2216 100 -0.4763108975657744 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 5.0 4 3.0 5 24.0 6 64.0 7 187.0 8 382.0 9 748.0 10 1255.0 11 2242.0 12 3435.0 13 5424.0 14 6492.0 15 8505.0 16 11051.0 17 15782.0 18 19014.0 19 10675.0 20 7353.0 21 6838.0 22 6992.0 23 7507.0 24 8589.0 25 10096.0 26 12765.0 27 16342.0 28 20878.0 29 26888.0 30 34136.0 31 43371.0 32 56197.0 33 72838.0 34 102570.0 35 158875.0 36 308135.0 37 726632.0 38 1344662.0 39 709413.0 40 2815.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.611233006241676 19.429780180187826 13.075821837015923 23.883164976554568 2 15.815754759931359 19.964809247229894 40.03088742036713 24.188548572471618 3 18.657192985528148 25.144600455430744 29.772372179724023 26.425834379317088 4 12.331750216983082 16.296351526656082 38.55296496668504 32.8189332896758 5 12.568048819249016 37.94136054923513 35.73177535950232 13.758815272013539 6 31.28931873048739 37.92470065961146 17.307285654133935 13.478694955767216 7 27.745053144439 32.386933753444396 22.098041952030716 17.76997115008588 8 25.59387455690223 35.980893990767946 19.87904912055288 18.546182331776944 9 25.440126458421332 16.10278089667044 20.98168708863339 37.47540555627485 10-11 24.213570668271256 26.444037813980092 28.608655232904272 20.733736284844376 12-13 25.14658670951589 23.81178904567048 28.854483282926775 22.187140961886858 14-15 22.03802949182581 25.706347269167303 26.345730371062142 25.90989286794475 16-17 21.333812481627344 29.30724071073133 26.870217139606726 22.4887296680346 18-19 21.194484163479967 27.86208206631489 29.56540852946003 21.37802524074511 20-21 22.170398504929974 27.084666511726134 29.341916614400766 21.403018368943126 22-23 21.853446509038363 26.854357183724275 29.41743218391994 21.874764123317426 24-25 21.718399313589025 26.978044638734172 29.359576596495167 21.943979451181633 26-27 21.522920512753842 27.012360366804618 29.515503125247083 21.949215995194457 28-29 21.53903321855238 27.13841213844398 29.551292970555476 21.771261672448166 30-31 21.721681056596825 27.392813185463126 29.15074314767389 21.734762610266152 32-33 21.506927521698966 27.163259238188214 29.53920938672656 21.790603853386266 34-35 21.669653465044377 26.99428832861098 29.504114510479535 21.831943695865107 36-37 21.88378410298807 27.345703013550292 29.009412617126902 21.761100266334733 38-39 21.746304264536267 27.457443619240212 29.123508889366352 21.672743226857172 40-41 21.61059719236093 27.2130408197205 29.276636556220403 21.899725431698165 42-43 21.644386130588643 27.19518790333222 29.00982612659564 22.1505998394835 44-45 21.694979942291592 27.346953735535717 28.952234394720406 22.005831927452288 46-47 21.897025498929782 27.542618516615452 28.668443525543353 21.89191245891141 48-49 21.872431104121574 27.478085616626647 28.49802111185864 22.15146216739314 50-51 21.8357479838956 27.541757704119117 28.363193086518912 22.25930122546637 52-53 21.904544622144233 27.552531187248853 28.311338242693694 22.23158594791322 54-55 21.972978514981715 27.826522448667358 28.175420255117327 22.0250787812336 56-57 21.843207668030292 27.560648761423597 28.276525633575993 22.31961793697012 58-59 21.793449396668933 27.131004100101975 28.680000849762067 22.395545653467032 60-61 21.95029168640631 27.54896316423453 28.351645170816063 22.149099978543095 62-63 21.74978715991394 27.499536611415877 28.419993637369355 22.330682591300828 64-65 21.7793461084987 27.32287431669633 28.467298526897572 22.4304810479074 66-67 21.659933341071515 27.380370437925944 28.36399091316099 22.595705307841545 68-69 22.009447132616963 27.476436556858697 28.270799053164264 22.243317257360076 70-71 22.0047262168934 27.334786696763334 28.20642005652146 22.454067029821807 72-73 21.769158237795512 27.45961464379065 28.32424384244033 22.446983275973498 74-75 21.86884814448099 27.55142486922913 28.10792404093402 22.471802945355872 76-77 21.892041970854724 27.46654649928486 28.02035293022076 22.621058599639657 78-79 21.90247443347184 27.534181120273715 28.101067445331694 22.462277000922747 80-81 21.855773327765366 27.394461073475433 28.17920777421496 22.570557824544238 82-83 21.751922962635945 27.440793551071003 28.261899657856983 22.545383828436076 84-85 21.732227206496464 27.40248032202161 28.128737542409667 22.736554929072256 86-87 21.85282618347797 27.485958061427816 27.99761113596932 22.663604619124893 88-89 21.533641049382922 27.42539300787243 28.069938520906806 22.971027421837846 90-91 21.57448611488072 27.320312991237618 28.133513956222295 22.97168693765937 92-93 21.704805444939 27.418743979911657 28.03279464887522 22.843655926274124 94-95 21.42452352627172 27.21913546509715 28.174974821896086 23.18136618673504 96-97 21.445227196691903 27.17957942565211 28.196153592068722 23.17903978558726 98-99 21.386538837053326 27.256947392326826 28.07766880046185 23.278844970157998 100 21.44252289883126 27.034841456568365 28.06461232498883 23.458023319611545 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 49.0 1 60.5 2 180.0 3 314.0 4 217.0 5 145.5 6 422.0 7 988.0 8 1691.0 9 1965.0 10 1609.0 11 1296.5 12 1328.5 13 1573.0 14 1837.0 15 2099.5 16 2390.5 17 2633.5 18 3040.0 19 3562.5 20 4267.5 21 5348.0 22 6900.5 23 9289.0 24 12437.5 25 16462.5 26 21999.0 27 29115.0 28 38192.5 29 48294.0 30 57793.0 31 68147.0 32 78337.5 33 87920.5 34 97156.5 35 105243.5 36 113383.0 37 120642.5 38 126083.5 39 128372.0 40 129969.5 41 132472.5 42 133235.0 43 133403.0 44 131717.5 45 128971.0 46 128301.5 47 127248.5 48 126487.5 49 123612.0 50 118411.0 51 114903.5 52 108364.5 53 102254.5 54 99078.0 55 94434.5 56 87545.5 57 79400.5 58 72400.5 59 67341.5 60 60682.5 61 51931.5 62 44953.0 63 38751.5 64 31730.5 65 25105.0 66 19931.5 67 15138.5 68 10971.0 69 8038.5 70 5729.0 71 4097.5 72 3087.5 73 2200.5 74 1578.0 75 1117.5 76 698.5 77 460.0 78 315.0 79 182.0 80 87.5 81 38.5 82 16.0 83 10.0 84 5.0 85 4.0 86 4.5 87 3.0 88 1.5 89 1.0 90 1.0 91 1.0 92 1.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.653091328954671E-4 2 4.2449461263274746E-4 3 2.3877821960592043E-4 4 0.012230751026481036 5 5.040873525013876E-4 6 0.004006167906721554 7 6.898037455282146E-4 8 0.14183426244591674 9 0.0011673601847400554 10-11 0.0 12-13 6.632728322386678E-5 14-15 2.1224730631637373E-4 16-17 0.0 18-19 1.1938910980296021E-4 20-21 0.0 22-23 1.1938910980296021E-4 24-25 4.6429098256706755E-4 26-27 0.005465368137646623 28-29 0.0071633465881776135 30-31 0.013862402193788158 32-33 0.016780802655638298 34-35 0.024912527578884364 36-37 0.03642694394654764 38-39 0.04161373749465402 40-41 0.05310162294902775 42-43 0.06890078181295281 44-45 0.1075032606492433 46-47 0.11420231625485383 48-49 0.11129718124964848 50-51 0.11286250513373172 52-53 0.10791448980523126 54-55 0.11493191637031637 56-57 0.09911949204974653 58-59 0.09094797075656613 60-61 0.0928581965134135 62-63 0.09153165084893616 64-65 0.0 66-67 1.3265456644773358E-5 68-69 5.306182657909343E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 2.6530913289546716E-5 78-79 5.306182657909343E-5 80-81 2.653091328954671E-4 82-83 1.0612365315818687E-4 84-85 0.0 86-87 8.622546819102682E-4 88-89 5.438837224357077E-4 90-91 2.3877821960592043E-4 92-93 9.683783350684551E-4 94-95 0.0010612365315818685 96-97 2.3877821960592043E-4 98-99 1.0612365315818687E-4 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3769188.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.552700608291964 #Duplication Level Percentage of deduplicated Percentage of total 1 73.52393994359497 42.3150130311576 2 15.345293123685973 17.663261217879604 3 5.017179377222127 8.662566677860854 4 2.180909269589674 5.020688729861727 5 1.122600430978761 3.2304343253430083 6 0.677639660519313 2.339999550130357 7 0.4483134995147092 1.8061156831357992 8 0.31063824113508276 1.4302455751627057 9 0.2227066474782063 1.1535632105210665 >10 1.0636382503420094 10.929824884420448 >50 0.0630587724018819 2.472139562934951 >100 0.023022326403678593 2.1561936093741756 >500 8.299229832525942E-4 0.3122193635138948 >1k 1.3832049018388924E-4 0.17773629184478731 >5k 9.221366012259283E-5 0.3299982868590288 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6864 0.18210818881944865 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5572 0.14783024884935428 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 2.6530913289546713E-5 0.0 8 0.0 0.0 0.0 2.6530913289546713E-5 0.0 9 0.0 0.0 0.0 2.6530913289546713E-5 0.0 10-11 0.0 0.0 0.0 2.6530913289546713E-5 0.0 12-13 0.0 0.0 0.0 2.6530913289546713E-5 0.0 14-15 0.0 0.0 0.0 2.6530913289546713E-5 0.0 16-17 0.0 0.0 0.0 2.6530913289546713E-5 0.0 18-19 0.0 0.0 0.0 9.28581965134135E-5 0.0 20-21 0.0 0.0 0.0 1.0612365315818685E-4 0.0 22-23 0.0 0.0 0.0 1.7245093638205365E-4 0.0 24-25 0.0 0.0 0.0 2.653091328954671E-4 0.0 26-27 0.0 0.0 0.0 3.846982426984274E-4 0.0 28-29 0.0 0.0 0.0 0.0011408292714505087 0.0 30-31 0.0 0.0 0.0 0.0040326988200111 0.0 32-33 0.0 0.0 0.0 0.011487885454373726 0.0 34-35 0.0 0.0 0.0 0.02252474538282516 0.0 36-37 0.0 0.0 0.0 0.043033141355644774 0.0 38-39 0.0 0.0 0.0 0.08216623845772617 0.0 40-41 0.0 0.0 0.0 0.12874125673752543 0.0 42-43 0.0 0.0 0.0 0.1772530316874616 0.0 44-45 0.0 0.0 0.0 0.23611186282032098 0.0 46-47 0.0 0.0 0.0 0.30706879041321367 0.0 48-49 0.0 0.0 0.0 0.38760337770363273 0.0 50-51 0.0 0.0 0.0 0.4759778498711128 0.0 52-53 0.0 0.0 0.0 0.5676421552864968 0.0 54-55 0.0 0.0 0.0 0.6655942871515032 0.0 56-57 0.0 0.0 0.0 0.7827548002381415 0.0 58-59 0.0 0.0 0.0 0.9192298181995697 0.0 60-61 0.0 0.0 0.0 1.0706682712563023 0.0 62-63 0.0 0.0 0.0 1.2235924554572497 0.0 64-65 0.0 0.0 0.0 1.3830565097840701 0.0 66-67 0.0 0.0 0.0 1.5653238840832562 0.0 68-69 0.0 0.0 0.0 1.7695721200428314 0.0 70-71 0.0 0.0 0.0 1.993493028206606 0.0 72-73 0.0 0.0 0.0 2.2262885268657335 0.0 74-75 0.0 0.0 0.0 2.4611799676747355 0.0 76-77 0.0 0.0 0.0 2.7092042105620626 0.0 78-79 0.0 0.0 0.0 2.9953003140198895 0.0 80-81 0.0 0.0 0.0 3.313512088014713 0.0 82-83 0.0 0.0 0.0 3.646090351555826 0.0 84-85 0.0 0.0 0.0 3.9675388969719743 0.0 86-87 0.0 0.0 0.0 4.3033937283043455 0.0 88 0.0 0.0 0.0 4.581358106838927 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 12160 0.0 34.811848 1 GGTATCA 7720 0.0 34.323982 1 TCAACGC 13665 0.0 30.534979 4 ATCAACG 13715 0.0 30.423658 3 CAACGCA 13900 0.0 30.04899 5 AACGCAG 14335 0.0 29.169567 6 TATCAAC 14530 0.0 28.813913 2 GTACATG 11705 0.0 27.29439 1 TACATGG 11790 0.0 26.01427 2 ACATGGG 11705 0.0 25.640879 3 ACGCAGA 16535 0.0 25.287518 7 CGCAGAG 16700 0.0 24.952848 8 GCAGAGT 18215 0.0 22.513956 9 GAGTACT 10540 0.0 21.481638 12-13 CATGGGG 7985 0.0 20.97291 4 AGAGTAC 14915 0.0 20.298344 10-11 GAGTACA 8730 0.0 20.127659 1 GTACTTT 11650 0.0 19.253445 14-15 GTCGTAC 300 4.26553E-9 18.79299 1 AGTACTT 11430 0.0 18.4322 12-13 >>END_MODULE