FastQCFastQC Report
Wed 25 May 2016
SRR1032937_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032937_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2403
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTCGTCGCCAAGTCCC150.6242197253433208No Hit
GTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCG140.5826050769870994No Hit
GTACAAGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACACGCCGC130.5409904286308781No Hit
TGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACT120.4993757802746567No Hit
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG110.4577611319184353RNA PCR Primer, Index 31 (96% over 29bp)
CTTCTGGTACTTTGTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGG100.41614648356221395No Hit
GTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACACGCCGCCCCTCTA100.41614648356221395No Hit
CAGTTAAAGAAGATGAAGAAGTCTTCAGGGGAGATTGTCTACTGTGGGCA100.41614648356221395No Hit
CCCCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTCGTCGCCAAGTC90.37453183520599254No Hit
ATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAA70.2913025384935497No Hit
GTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTT60.24968789013732834No Hit
ACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGG60.24968789013732834No Hit
CCATGAAGGCCTCGGGCACGCTACGAGAGTACAAGGTAGTGGGTCGCTGC50.20807324178110698No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.20807324178110698No Hit
CGCTACGAGAGTACAAGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGC50.20807324178110698No Hit
GATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGA50.20807324178110698No Hit
ACAAGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACACGCCGCCC50.20807324178110698No Hit
CTGCCTGCCCACCCCCAAATGCCACACGCCGCCCCTCTACCGCATGCGAA50.20807324178110698No Hit
CTTCAGGGGAGATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTG50.20807324178110698No Hit
GGTACTTTGTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGGAGATT40.16645859342488556No Hit
CTATGACTCCCGGAGCGGCACCCACAACATGTACCGGGAATACCGGGACC40.16645859342488556No Hit
AGATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAG40.16645859342488556No Hit
CTACGAGAGTACAAGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCA40.16645859342488556No Hit
CCCACAACATGTACCGGGAATACCGGGACCTGACCACCGCAGGCGCTGTC40.16645859342488556No Hit
TTGTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGGAGATTGTCTAC40.16645859342488556No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT40.16645859342488556No Hit
GCCACACGCCGCCCCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTC40.16645859342488556No Hit
GGGGTAGGAAATCCTCCAGGATCTTAGGAAATTTCACTCCGCTTCTCCTG40.16645859342488556No Hit
GCCACGAGGTGTGTTTTATTTTCATTATTCATACAAATAATTTTCTATAA30.12484394506866417No Hit
CAACATGTACCGGGAATACCGGGACCTGACCACCGCAGGCGCTGTCACCC30.12484394506866417No Hit
GTGCCCAGGTTCACAGTGCAGCTTGTGGCTCGTGTCCATCTTGCAGGTGG30.12484394506866417No Hit
CCTCGGGCACGCTACGAGAGTACAAGGTAGTGGGTCGCTGCCTGCCCACC30.12484394506866417No Hit
CGAGAGTACAAGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACAC30.12484394506866417No Hit
GTACATGGGGTCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATG30.12484394506866417No Hit
ACTTTGTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGGAGATTGTC30.12484394506866417No Hit
CTCCCGGAGCGGCACCCACAACATGTACCGGGAATACCGGGACCTGACCA30.12484394506866417No Hit
GTACATGGGGCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGA30.12484394506866417No Hit
GTCCCGCTTCTGGTACTTTGTATCTCAGTTAAAGAAGATGAAGAAGTCTT30.12484394506866417No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.12484394506866417No Hit
GCCAAGTCCCGCTTCTGGTACTTTGTATCTCAGTTAAAGAAGATGAAGAA30.12484394506866417No Hit
AGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACACGCCGCCCCTC30.12484394506866417No Hit
GTTCAGGTCCCCAAGCTAACCCCCTACTTCCCAGACAGCTGCTCGTACAG30.12484394506866417No Hit
CCCCCAAATGCCACACGCCGCCCCTCTACCGCATGCGAATCTTTGCGCCT30.12484394506866417No Hit
GGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGC30.12484394506866417No Hit
GTGAAGAACTTCGGGATCTGGCTGCGCTATGACTCCCGGAGCGGCACCCA30.12484394506866417No Hit
GGCCTCGGGCACGCTACGAGAGTACAAGGTAGTGGGTCGCTGCCTGCCCA30.12484394506866417No Hit
GGTTCACAGTGCAGCTTGTGGCTCGTGTCCATCTTGCAGGTGGCTCTTCC30.12484394506866417No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)