FastQCFastQC Report
Wed 25 May 2016
SRR1032936_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032936_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2953569
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA43800.036.8077621
GGTATCA27200.033.523721
TCAACGC46650.033.0482834
ATCAACG46850.032.9066473
CAACGCA48850.031.6550755
AACGCAG50400.030.7748136
GTACATG106500.030.3198131
TACATGG106700.028.5449962
TATCAAC57200.027.5275882
ACATGGG109450.026.539463
ACGCAGA60300.025.4879637
CGCAGAG64350.023.372558
CATGGGG73800.022.1003864
GAGTACA72850.020.7107051
GCAGAGT73400.019.9785169
AGTACAT74850.018.838612
GGACCGT4303.6379788E-1217.4889436
TATACTG16350.017.248275
CATGGGA34450.017.0548344
GTATAGA13900.016.9073121
Produced by FastQC (version 0.11.5)