FastQCFastQC Report
Wed 25 May 2016
SRR1032936_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032936_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2953569
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73110.24753103787316294No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59020.199826040969417No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33670.11399767535479957No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47950.052.7098581
GTATCAA92200.044.1759951
ATCAACG109800.036.5951353
TCAACGC110300.036.3865934
CAACGCA112350.035.8027765
TATCAAC116150.034.7165682
AACGCAG117150.034.3759846
ACGCAGA135500.029.719137
CGCAGAG139200.028.8953848
GTACATG110900.026.5180131
GCAGAGT156000.025.5627619
TACATGG108900.025.4917342
ACATGGG113650.023.3494953
GAGTACT95100.021.7071612-13
CAGAGTA149700.021.25749810-11
AGAGTAC141250.020.88314210-11
CATGGGG74500.020.7150234
GTACTTT102400.019.99913214-15
GTGGTAT21500.019.8838791
AGTACTT99500.019.51987812-13
Produced by FastQC (version 0.11.5)