FastQCFastQC Report
Wed 25 May 2016
SRR1032935_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032935_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1637287
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG55700.029.532861
TACATGG55900.027.9137952
GTATCAA30300.027.610231
GTCTCGC3300.027.060281
ATCAACG30050.026.745923
TCAACGC30300.026.3701254
AACGCAG30900.026.0093966
CAACGCA31050.025.732385
ACATGGG56800.025.6518843
TATCAAC34700.023.838332
GGTATCA19950.023.3230291
CGAACTA853.45634E-622.11670528-29
CATGGGG39450.021.8026834
GTACACG1805.667722E-620.8886361
ACGCAGA38250.020.7657627
GAGTACA39750.020.6915761
CGCAGAG39950.019.7644678
TACGCGA600.00506555119.58369480-81
CGTACAC1456.600711E-419.4486353
AGTACAT40450.018.590632
Produced by FastQC (version 0.11.5)