FastQCFastQC Report
Wed 25 May 2016
SRR1032935_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032935_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1637287
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42760.26116374221501787No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37270.2276326630578512No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19530.11928269142795367No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35750.037.4507061
GTATCAA58750.035.663031
ATCAACG66150.031.2904193
TCAACGC66350.031.1960984
GTACATG54250.030.6545371
CAACGCA67550.030.6381655
TATCAAC69400.029.9606572
AACGCAG69550.029.7571246
TACATGG56300.028.5763172
ACATGGG56850.026.6448923
GTCGTAC1458.690222E-725.91871
ACGCAGA80300.025.6562967
CGCAGAG81900.025.0976458
GCAGAGT89850.022.7932669
CATGGGG37500.021.0749514
GAGTACT55050.020.77685512-13
CATGGGA19050.020.0022354
AGAGTAC75550.019.5845810-11
GTACTTT59050.019.1308114-15
ACACCGT3759.094947E-1218.814626
Produced by FastQC (version 0.11.5)