FastQCFastQC Report
Wed 25 May 2016
SRR1032934_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032934_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4448110
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC42050.032.0790144
GTATCAA46250.031.0967371
GTACATG123350.030.8639131
ATCAACG43750.030.8325183
GGTATCA29700.030.7008531
CAACGCA44650.030.2110335
TACATGG126050.029.0468982
ACATGGG127200.028.2669833
AACGCAG48050.027.9751786
TATCAAC56150.024.6932012
CATGGGG101750.022.6342874
ACGCAGA59550.022.4143967
GAGTACA82500.022.1615851
CGCAGAG64000.021.0025338
ACACCGT7400.020.3244366
TACACCG7450.020.1882575
TATACCG2109.538926E-720.1431965
AGTACAT86700.019.7869532
TACGACC4600.019.4133684
ACCGTTC4700.017.999498
Produced by FastQC (version 0.11.5)