##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032934_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4448110 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.97176576118846 33.0 31.0 34.0 30.0 34.0 2 32.06668697491744 34.0 31.0 34.0 30.0 34.0 3 32.17601543127306 34.0 31.0 34.0 30.0 34.0 4 35.520595488870555 37.0 35.0 37.0 35.0 37.0 5 35.55416412813532 37.0 35.0 37.0 35.0 37.0 6 35.66187841577659 37.0 37.0 37.0 35.0 37.0 7 35.613213027555524 37.0 37.0 37.0 35.0 37.0 8 35.61638111467567 37.0 37.0 37.0 35.0 37.0 9 37.337808192693075 39.0 39.0 39.0 35.0 39.0 10-11 37.32228440843414 39.0 39.0 39.0 35.0 39.0 12-13 37.27113672998195 39.0 39.0 39.0 35.0 39.0 14-15 38.796514699501586 41.0 40.0 41.0 36.0 41.0 16-17 38.81015768494934 41.0 40.0 41.0 36.0 41.0 18-19 38.81772381977964 41.0 40.0 41.0 36.0 41.0 20-21 38.84763281483596 41.0 40.0 41.0 36.0 41.0 22-23 38.826767211242526 41.0 40.0 41.0 36.0 41.0 24-25 38.842939360762216 41.0 39.5 41.0 36.0 41.0 26-27 38.807697876176626 41.0 39.0 41.0 35.0 41.0 28-29 38.78202079085274 41.0 39.0 41.0 35.0 41.0 30-31 38.737106209154 41.0 39.0 41.0 35.0 41.0 32-33 38.68751863150867 41.0 39.0 41.0 35.0 41.0 34-35 38.623046979503656 41.0 39.0 41.0 35.0 41.0 36-37 38.54044796554042 41.0 39.0 41.0 35.0 41.0 38-39 38.45596028425556 41.0 39.0 41.0 34.0 41.0 40-41 38.37839329512984 41.0 39.0 41.0 34.0 41.0 42-43 38.27539022191448 41.0 38.5 41.0 34.0 41.0 44-45 38.15280703489797 40.0 38.0 41.0 34.0 41.0 46-47 38.03827468295523 40.0 38.0 41.0 33.0 41.0 48-49 37.913068921407074 40.0 38.0 41.0 33.0 41.0 50-51 37.77926580053101 40.0 38.0 41.0 33.0 41.0 52-53 37.62553500250668 40.0 37.5 41.0 33.0 41.0 54-55 37.46172183241872 40.0 37.0 41.0 33.0 41.0 56-57 37.282421073219865 40.0 36.5 41.0 32.0 41.0 58-59 37.07767681105008 40.0 36.0 41.0 32.0 41.0 60-61 36.83298681912093 39.0 35.5 41.0 31.5 41.0 62-63 36.579737236714024 39.0 35.0 41.0 31.0 41.0 64-65 36.29937276731016 39.0 35.0 41.0 31.0 41.0 66-67 35.99565995445256 38.0 35.0 40.0 31.0 41.0 68-69 35.6780289830962 37.0 35.0 40.0 30.5 41.0 70-71 35.33511806137888 37.0 35.0 39.5 30.0 41.0 72-73 35.034549730110086 36.0 35.0 39.0 30.0 41.0 74-75 34.7428784360099 36.0 35.0 39.0 30.0 41.0 76-77 34.41364287304046 35.5 34.0 38.5 30.0 40.0 78-79 34.04495268777076 35.0 34.0 37.0 29.0 39.0 80-81 33.723285620184754 35.0 34.0 37.0 29.0 39.0 82-83 33.41118194019482 35.0 34.0 36.5 29.0 39.0 84-85 33.24443808718759 35.0 34.0 36.0 29.0 37.0 86-87 33.016561753194054 35.0 34.0 36.0 29.0 37.0 88-89 32.79306413285643 35.0 34.0 35.5 29.0 37.0 90-91 32.60836231568014 35.0 34.0 35.0 29.0 36.0 92-93 32.42875457216661 35.0 34.0 35.0 29.0 36.0 94-95 32.25924628662511 35.0 34.0 35.0 28.0 36.0 96-97 32.0982486943893 35.0 34.0 35.0 27.5 36.0 98-99 31.93083051003685 35.0 34.0 35.0 27.0 35.0 100 31.812723381391198 35.0 34.0 35.0 27.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2399484972012118 1101 2 0.3250396888273315 1101 3 0.30273149755638684 1101 4 0.2969416740672841 1101 5 0.2633437503692875 1101 6 0.240844874032625 1101 7 0.28096503338238676 1101 8 0.2604056658973448 1101 9 0.31415795608318575 1101 10-11 0.33268140779001953 1101 12-13 0.18189657738222564 1101 14-15 0.2956180498667109 1101 16-17 0.2679517812124459 1101 18-19 0.27855768981427786 1101 20-21 0.23615905603464427 1101 22-23 0.28165329358414226 1101 24-25 0.26765508006607774 1101 26-27 0.307378241040027 1101 28-29 0.2787406721643535 1101 30-31 0.32700623656033656 1101 32-33 0.325129663975261 1101 34-35 0.31575239855496307 1101 36-37 0.3275340160944964 1101 38-39 0.30523427457594465 1101 40-41 0.316062764331015 1101 42-43 0.29549321749420443 1101 44-45 0.31469715980254875 1101 46-47 0.26826172933150616 1101 48-49 0.31169431732363506 1101 50-51 0.33806499686678393 1101 52-53 0.3100798273028218 1101 54-55 0.33167043423786424 1101 56-57 0.36685507397614003 1101 58-59 0.3403869617285835 1101 60-61 0.4280211600306032 1101 62-63 0.4321401173530077 1101 64-65 0.3934194742166852 1101 66-67 0.374184406308423 1101 68-69 0.3898036351493772 1101 70-71 0.4305347502078689 1101 72-73 0.3581697955210359 1101 74-75 0.3932930503118115 1101 76-77 0.33226234469590565 1101 78-79 0.36840595443545965 1101 80-81 0.3834064989126986 1101 82-83 0.3108088922990646 1101 84-85 0.2291960194087821 1101 86-87 0.1774751827911203 1101 88-89 0.20175688742115483 1101 90-91 0.2248329535492033 1101 92-93 0.26250582686462565 1101 94-95 0.27900578587916414 1101 96-97 0.250014819730751 1101 98-99 0.1903642995737158 1101 100 0.19964662610162875 1102 1 0.31799204933075487 1102 2 0.318468202005171 1102 3 0.33400301391998966 1102 4 0.2872101960659563 1102 5 0.2842965381521694 1102 6 0.2787695669333843 1102 7 0.2820711038019752 1102 8 0.28491113799871215 1102 9 0.35745394884899895 1102 10-11 0.35720260758864697 1102 12-13 0.3454414117213105 1102 14-15 0.4394573555180088 1102 16-17 0.4451242352741218 1102 18-19 0.42318442051976035 1102 20-21 0.4019344218006893 1102 22-23 0.4053327148492656 1102 24-25 0.4065375536694802 1102 26-27 0.4537605100724136 1102 28-29 0.42568691922235047 1102 30-31 0.43148333223439295 1102 32-33 0.41671086618713105 1102 34-35 0.4052799157839999 1102 36-37 0.42493012522307794 1102 38-39 0.4105529684836142 1102 40-41 0.4244767633684887 1102 42-43 0.45393685505815995 1102 44-45 0.40665310838102187 1102 46-47 0.4088835836186888 1102 48-49 0.45317316468037205 1102 50-51 0.47978824764588524 1102 52-53 0.42394712104321997 1102 54-55 0.40084231688760497 1102 56-57 0.4427027399941892 1102 58-59 0.4115938336504854 1102 60-61 0.44752315789197183 1102 62-63 0.39846068221866204 1102 64-65 0.3547698093664309 1102 66-67 0.3950319683073147 1102 68-69 0.4229736537757418 1102 70-71 0.4165482505081286 1102 72-73 0.33017489662073274 1102 74-75 0.3404495547686821 1102 76-77 0.30486053531726043 1102 78-79 0.3577145876098058 1102 80-81 0.31440669169487734 1102 82-83 0.3565267146648452 1102 84-85 0.313768479850296 1102 86-87 0.3103337957857448 1102 88-89 0.3222677047205167 1102 90-91 0.2974977719109404 1102 92-93 0.32795163218790435 1102 94-95 0.35788545789981185 1102 96-97 0.3888233981216729 1102 98-99 0.38695969693384313 1102 100 0.3691831583246845 1103 1 0.09345413413655734 1103 2 0.17246757001891666 1103 3 0.2294344655754088 1103 4 0.1336548860796043 1103 5 0.0501161255514333 1103 6 0.07628909083277335 1103 7 0.0917291695453315 1103 8 0.13734194497220642 1103 9 0.17996339690358099 1103 10-11 0.19686728422664856 1103 12-13 0.17433894807235362 1103 14-15 0.1970437726982155 1103 16-17 0.20425378180408416 1103 18-19 0.14968830894589047 1103 20-21 0.1551706750238253 1103 22-23 0.149781518798612 1103 24-25 0.1665596301520793 1103 26-27 0.19928255556897057 1103 28-29 0.2305532563897046 1103 30-31 0.2396162362316545 1103 32-33 0.22132826597147925 1103 34-35 0.2098967238209184 1103 36-37 0.20058938296092776 1103 38-39 0.2015678836388659 1103 40-41 0.2553199652557083 1103 42-43 0.28637326964544485 1103 44-45 0.23751686004248995 1103 46-47 0.2698191997773094 1103 48-49 0.2253823466422702 1103 50-51 0.19405165228513965 1103 52-53 0.10823596329046126 1103 54-55 0.17786676283449054 1103 56-57 0.14289308631275333 1103 58-59 0.19185056542299606 1103 60-61 0.18274175100493295 1103 62-63 0.22347857450900932 1103 64-65 0.1992096859989232 1103 66-67 0.2054093030467783 1103 68-69 0.23210108346377467 1103 70-71 0.19404813861407177 1103 72-73 0.1653305385666286 1103 74-75 0.1527563088877315 1103 76-77 0.20509491840335414 1103 78-79 0.18072819102241056 1103 80-81 0.16245227367632964 1103 82-83 0.18383273286527668 1103 84-85 0.19347431090790934 1103 86-87 0.22774730598198545 1103 88-89 0.14265099082523136 1103 90-91 0.16583815223884812 1103 92-93 0.17711597992193617 1103 94-95 0.147572612870718 1103 96-97 0.1366175116549826 1103 98-99 0.14436101820901825 1103 100 0.21780725410771495 1104 1 0.03657007631413478 1104 2 0.09124020111780595 1104 3 0.1168491446152089 1104 4 0.005461873590945743 1104 5 0.03405781139476716 1104 6 0.029480114224959664 1104 7 0.02625780289660895 1104 8 0.08648664374302228 1104 9 0.12329649920096131 1104 10-11 0.10305618871844047 1104 12-13 0.09854511915187913 1104 14-15 0.11547964156221013 1104 16-17 0.08167712161598928 1104 18-19 0.09702621091255281 1104 20-21 0.08807160715134899 1104 22-23 0.1491444183763022 1104 24-25 0.1759703633986618 1104 26-27 0.22432966332017656 1104 28-29 0.16701146711334758 1104 30-31 0.15146765749234703 1104 32-33 0.22079361939699993 1104 34-35 0.23749148991342395 1104 36-37 0.2275259272974779 1104 38-39 0.19174710430677067 1104 40-41 0.21809215362684142 1104 42-43 0.17251743894748728 1104 44-45 0.16020383306008767 1104 46-47 0.12375671877113348 1104 48-49 0.13550279258539888 1104 50-51 0.15595112457989302 1104 52-53 0.16277519421964115 1104 54-55 0.13855444987620302 1104 56-57 0.15926194046605247 1104 58-59 0.12320051495736806 1104 60-61 0.18920167501735108 1104 62-63 0.16480807223549476 1104 64-65 0.14271350198822574 1104 66-67 0.21279223446641993 1104 68-69 0.1905851028166552 1104 70-71 0.12237045775290767 1104 72-73 0.1627071612694806 1104 74-75 0.2199631622068381 1104 76-77 0.18960562425371563 1104 78-79 0.19925572749977505 1104 80-81 0.10603580732055917 1104 82-83 0.125426247262439 1104 84-85 0.1570196836338269 1104 86-87 0.20437267975781737 1104 88-89 0.17864494745801096 1104 90-91 0.1789168972871238 1104 92-93 0.14150641105551642 1104 94-95 0.19101540256005478 1104 96-97 0.14254391693113178 1104 98-99 0.15716113595532732 1104 100 0.1744230607396524 1105 1 0.08345720540817325 1105 2 0.09395949756589062 1105 3 0.13632683380759403 1105 4 0.1962204223063182 1105 5 0.13166185871790503 1105 6 0.0808748656526106 1105 7 0.12461295874580003 1105 8 0.1097046766408809 1105 9 0.1343644727744504 1105 10-11 0.1856538471516913 1105 12-13 0.24012652697243908 1105 14-15 0.3047708384906471 1105 16-17 0.27032765715714646 1105 18-19 0.2812873163978509 1105 20-21 0.2560918602770528 1105 22-23 0.26587762363724465 1105 24-25 0.26633394401671495 1105 26-27 0.30054513298976815 1105 28-29 0.3084408856860037 1105 30-31 0.27380232208315647 1105 32-33 0.2950903583487232 1105 34-35 0.25701674251421025 1105 36-37 0.270215749485601 1105 38-39 0.3027268609899494 1105 40-41 0.2989120720071554 1105 42-43 0.31987111190606043 1105 44-45 0.3558145292572732 1105 46-47 0.2974348323485714 1105 48-49 0.280834522958223 1105 50-51 0.2786525057202809 1105 52-53 0.34800109151705527 1105 54-55 0.3079994443846843 1105 56-57 0.2907622608088829 1105 58-59 0.31399290920173684 1105 60-61 0.2912404335380998 1105 62-63 0.2864357683086709 1105 64-65 0.2740448617597906 1105 66-67 0.26506493017588895 1105 68-69 0.2546821249830984 1105 70-71 0.22245229400833466 1105 72-73 0.26158468911046384 1105 74-75 0.26566931542113537 1105 76-77 0.20402513069946338 1105 78-79 0.21732394707247238 1105 80-81 0.20252275795105135 1105 82-83 0.15411943788190996 1105 84-85 0.17283115422837625 1105 86-87 0.14875185029665516 1105 88-89 0.16106435600908497 1105 90-91 0.21267366068725835 1105 92-93 0.17677753122404738 1105 94-95 0.16694557432927581 1105 96-97 0.1937329244471684 1105 98-99 0.23014758676941938 1105 100 0.2189683900032371 1106 1 0.05713670421478412 1106 2 0.05839946783508765 1106 3 0.026042088619739445 1106 4 0.05165164699877067 1106 5 0.048842901952994566 1106 6 0.0492025319996543 1106 7 0.100730860386264 1106 8 0.09632377352500754 1106 9 0.06886954504569331 1106 10-11 0.09916265172991245 1106 12-13 0.10988073017796296 1106 14-15 0.0906099192106069 1106 16-17 0.09764804313067543 1106 18-19 0.12861518516818649 1106 20-21 0.12845207781066392 1106 22-23 0.15031117981556008 1106 24-25 0.17770542953277868 1106 26-27 0.20614268431734217 1106 28-29 0.1919020076119935 1106 30-31 0.1384433426527778 1106 32-33 0.15407022938411785 1106 34-35 0.12688127197932886 1106 36-37 0.16198269700917223 1106 38-39 0.16236660226663702 1106 40-41 0.1846866129089051 1106 42-43 0.1314066790326649 1106 44-45 0.12055726706660863 1106 46-47 0.13752108211107839 1106 48-49 0.1847550548397976 1106 50-51 0.19611682096179806 1106 52-53 0.21917320864574208 1106 54-55 0.24070798675887062 1106 56-57 0.21599521921692144 1106 58-59 0.20100504309574774 1106 60-61 0.14636702602123108 1106 62-63 0.17225806236755403 1106 64-65 0.18254828198979567 1106 66-67 0.15863998060545015 1106 68-69 0.16607552731970543 1106 70-71 0.13094590385200888 1106 72-73 0.12275139498774479 1106 74-75 0.09766519700703213 1106 76-77 0.14916347894768478 1106 78-79 0.16758215736855675 1106 80-81 0.11863428353616712 1106 82-83 0.16754438337049038 1106 84-85 0.14480533885770797 1106 86-87 0.19366692992266366 1106 88-89 0.16118657997125752 1106 90-91 0.13044126297874215 1106 92-93 0.14382627571724527 1106 94-95 0.1994933979913185 1106 96-97 0.21669945632577736 1106 98-99 0.15269681310797267 1106 100 0.1608992521520456 1107 1 0.1490291948068858 1107 2 0.12403550826063281 1107 3 0.14064459244258387 1107 4 0.17715788115313558 1107 5 0.16023999630484553 1107 6 0.13654184628886412 1107 7 0.17265035072540513 1107 8 0.18021732925615197 1107 9 0.16503080773640733 1107 10-11 0.12259948557390032 1107 12-13 0.1613678834926091 1107 14-15 0.2103919192134427 1107 16-17 0.17562713276010555 1107 18-19 0.22485472118964367 1107 20-21 0.2187070903237327 1107 22-23 0.21640309300139648 1107 24-25 0.22754824336153945 1107 26-27 0.20339572340986933 1107 28-29 0.2150769755299251 1107 30-31 0.19108418934949611 1107 32-33 0.183134815784733 1107 34-35 0.19852424096026766 1107 36-37 0.22544964465748052 1107 38-39 0.1500313692932309 1107 40-41 0.11309287285683922 1107 42-43 0.13265549949242939 1107 44-45 0.1834472125874811 1107 46-47 0.16332148187574091 1107 48-49 0.20462198085135697 1107 50-51 0.14152937759394746 1107 52-53 0.08902961827448763 1107 54-55 0.07625174414875602 1107 56-57 0.1212514254815602 1107 58-59 0.12050182174294122 1107 60-61 0.20147245009437142 1107 62-63 0.22723407453387523 1107 64-65 0.189831365606409 1107 66-67 0.14661082411172544 1107 68-69 0.11737341683089397 1107 70-71 0.16699393689761166 1107 72-73 0.1400937007615255 1107 74-75 0.13279011546953257 1107 76-77 0.0858800128686994 1107 78-79 0.08826786905055428 1107 80-81 0.08100541787727167 1107 82-83 0.04878912962012549 1107 84-85 0.09029647969339294 1107 86-87 0.09293905678293157 1107 88-89 0.17317405730418045 1107 90-91 0.20866851792570884 1107 92-93 0.2051412730028801 1107 94-95 0.11696237555020872 1107 96-97 0.14425070503965287 1107 98-99 0.16409893915819396 1107 100 0.1470686099705496 1108 1 -7.291010217080895E-4 1108 2 0.03961275010718879 1108 3 0.05794930909726048 1108 4 0.028049614504922715 1108 5 -0.04124987722300233 1108 6 0.01463723906165626 1108 7 0.006804461110817783 1108 8 0.03238330215219065 1108 9 0.03427592774576027 1108 10-11 0.033654855687487384 1108 12-13 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-0.05387386976414632 2214 58-59 -0.07854447570433365 2214 60-61 -0.041591020649192956 2214 62-63 -0.07031634411563203 2214 64-65 -0.14819471535471962 2214 66-67 -0.09915811831340449 2214 68-69 -0.14993931706094088 2214 70-71 -0.10376993455317063 2214 72-73 -0.1399572034228953 2214 74-75 -0.12303340962972698 2214 76-77 -0.15630308301088292 2214 78-79 -0.10877806992933614 2214 80-81 -0.08765305160317638 2214 82-83 -0.10137473415894505 2214 84-85 -0.1167141224826338 2214 86-87 -0.12918448645154257 2214 88-89 -0.04585762907906599 2214 90-91 -0.07301903802808596 2214 92-93 -0.13011708869525762 2214 94-95 -0.1328923800931392 2214 96-97 -0.21621698141048284 2214 98-99 -0.23482560950996145 2214 100 -0.23379496655796217 2215 1 -0.23942991057088392 2215 2 -0.28936518864667704 2215 3 -0.2863427562920684 2215 4 -0.24721491895830638 2215 5 -0.2969782863744328 2215 6 -0.2964869466470077 2215 7 -0.3546764176390198 2215 8 -0.3353571380454312 2215 9 -0.3902413010759389 2215 10-11 -0.3864172355139317 2215 12-13 -0.38370859706129323 2215 14-15 -0.4240057539916009 2215 16-17 -0.42718309070380656 2215 18-19 -0.41102897200022426 2215 20-21 -0.39073699733645384 2215 22-23 -0.4042071203171176 2215 24-25 -0.41912896324475923 2215 26-27 -0.3801268396620614 2215 28-29 -0.40163094639247987 2215 30-31 -0.37603605344165203 2215 32-33 -0.4475729462908262 2215 34-35 -0.4576683920221001 2215 36-37 -0.4711980867478829 2215 38-39 -0.42805364091287146 2215 40-41 -0.42226750163813165 2215 42-43 -0.4362960863896532 2215 44-45 -0.44905236184504815 2215 46-47 -0.3945419944659889 2215 48-49 -0.33669219350355206 2215 50-51 -0.33950469138822115 2215 52-53 -0.35687198084320215 2215 54-55 -0.3339053143640953 2215 56-57 -0.3535371176442794 2215 58-59 -0.31925380734737274 2215 60-61 -0.3563638012997501 2215 62-63 -0.37290073159607573 2215 64-65 -0.38844111069229825 2215 66-67 -0.38034596372518337 2215 68-69 -0.329572395198241 2215 70-71 -0.3147691169650102 2215 72-73 -0.3370656685616069 2215 74-75 -0.323288515681007 2215 76-77 -0.27141242363555307 2215 78-79 -0.3067374470124662 2215 80-81 -0.29572285279075317 2215 82-83 -0.3141883601073019 2215 84-85 -0.28494754991869087 2215 86-87 -0.2491381551858396 2215 88-89 -0.25479272932341956 2215 90-91 -0.22980791458611805 2215 92-93 -0.2525321128676694 2215 94-95 -0.26621751997127774 2215 96-97 -0.22221425554496932 2215 98-99 -0.27056522126055427 2215 100 -0.25750040196136936 2216 1 -0.011077170864510322 2216 2 -0.07557611575911949 2216 3 -0.1359261139285053 2216 4 -0.0851805688779379 2216 5 -0.05432158754788219 2216 6 -0.050222072645652815 2216 7 -0.05500320116851043 2216 8 -0.10874014803956555 2216 9 -0.1313716906984439 2216 10-11 -0.18296740462128014 2216 12-13 -0.16116296124139495 2216 14-15 -0.20437534153789017 2216 16-17 -0.24793769254048215 2216 18-19 -0.2074017265804713 2216 20-21 -0.2649961826832907 2216 22-23 -0.22940256511645885 2216 24-25 -0.2621686271149031 2216 26-27 -0.2893021249034007 2216 28-29 -0.2545706760393216 2216 30-31 -0.251794393767085 2216 32-33 -0.30065901363274605 2216 34-35 -0.261385015033639 2216 36-37 -0.2523874842374383 2216 38-39 -0.24477876183259184 2216 40-41 -0.22506267544690672 2216 42-43 -0.18889425710840158 2216 44-45 -0.17783481605700047 2216 46-47 -0.08896546272853811 2216 48-49 -0.13896532695716957 2216 50-51 -0.1475334676893496 2216 52-53 -0.17614081943562354 2216 54-55 -0.11410248518448185 2216 56-57 -0.08597243063631765 2216 58-59 -0.062325123464042065 2216 60-61 -0.058789720650104016 2216 62-63 -0.1304221294890766 2216 64-65 -0.1273748628799325 2216 66-67 -0.12987175470026813 2216 68-69 -0.10858424922631116 2216 70-71 -0.09525160736134097 2216 72-73 -0.14547128945364562 2216 74-75 -0.15719692983226707 2216 76-77 -0.17061825967923028 2216 78-79 -0.12277699418320509 2216 80-81 -0.0799524854124698 2216 82-83 -0.09236701987290274 2216 84-85 -0.09492669959635691 2216 86-87 -0.13334819998615188 2216 88-89 -0.10955285985531305 2216 90-91 -0.12487706168672474 2216 92-93 -0.1685499514883162 2216 94-95 -0.16363532219547494 2216 96-97 -0.17589438941723046 2216 98-99 -0.14594759198104157 2216 100 -0.21726515115857836 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 42341.0 3 6209.0 4 4086.0 5 3713.0 6 3282.0 7 4027.0 8 4537.0 9 4848.0 10 5087.0 11 5627.0 12 6076.0 13 6732.0 14 7249.0 15 7802.0 16 7587.0 17 7830.0 18 7657.0 19 7340.0 20 7341.0 21 7620.0 22 9021.0 23 11660.0 24 17504.0 25 23698.0 26 25030.0 27 23485.0 28 26802.0 29 33953.0 30 41766.0 31 51939.0 32 67463.0 33 91694.0 34 134251.0 35 213007.0 36 411458.0 37 904924.0 38 1516225.0 39 686267.0 40 972.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.010567125817374 18.87490078133015 12.712633933955015 24.40189815889746 2 15.610656829464089 19.54874023089467 40.25546367926736 24.585139260373882 3 18.594751433548293 24.673680174847217 29.358164554199444 27.373403837405046 4 12.33363573200278 15.95708728881593 38.671659844696684 33.037617134484606 5 13.038153711018532 38.08121770762741 35.767304121813964 13.113324459540094 6 31.85254267033259 37.4870740279084 17.583596952665516 13.076786349093494 7 28.452657801329078 31.765347245721525 22.180585954655744 17.60140899829365 8 25.69506161450939 36.591818781119436 19.9124774725114 17.800642131859775 9 25.38992954347819 16.684234861072518 20.48434218267847 37.44149341277082 10-11 24.45754050228879 26.542241367575826 28.67518060421485 20.325037525920532 12-13 25.021478081220167 23.688402736099995 28.604911863089256 22.685207319590585 14-15 22.0908866741272 25.817148969046883 25.354712848182633 26.737251508643283 16-17 22.153917663534127 29.193269391177125 26.100913235999336 22.55189970928941 18-19 22.075814560727895 28.29038707762903 27.767092428285604 21.866705933357466 20-21 22.976630419376363 27.654832414589688 27.434801898291905 21.93373526774204 22-23 22.750583159645352 27.31127630967937 27.51294110447557 22.425199426199708 24-25 22.613619195776767 27.418559227816825 27.35011890999007 22.617702666416335 26-27 22.331530096506356 27.464399290404373 27.465726688692477 22.73834392439679 28-29 22.423118850410738 27.493758944818758 27.573444674380195 22.509677530390306 30-31 22.54033935477281 27.91776506509171 27.15954111038041 22.382354469755075 32-33 22.337512008441344 27.596613113330438 27.629460559441505 22.436414318786717 34-35 22.46824261855382 27.3594536134184 27.649965584241663 22.52233818378611 36-37 22.66771367318751 27.663007880557565 27.183407353814754 22.485871092440167 38-39 22.427252493008687 27.93544313636471 27.293933010504773 22.34337136012183 40-41 22.343110796740824 27.503234350563442 27.631247251744117 22.522407600951617 42-43 22.32367776959189 27.466895796610725 27.527406890930074 22.682019542867312 44-45 22.289882567240234 27.639447183607203 27.631561184776267 22.4391090643763 46-47 22.36381106602483 27.725114617888437 27.470785699395528 22.44028861669121 48-49 22.25659216657779 27.578072088005058 27.518276260100038 22.647059485317115 50-51 22.316508889692923 27.526670428832954 27.473894346130102 22.68292633534402 52-53 22.421393794487336 27.539116906775103 27.515794594443776 22.52369470429378 54-55 22.46691108802004 27.897099570275042 27.282269761430417 22.353719580274504 56-57 22.291797380249484 27.56704038797985 27.451399297693285 22.68976293407738 58-59 22.201218252730744 27.237715884130992 27.815870675848565 22.745195187289706 60-61 22.51514767314933 27.648667356883895 27.314827366756784 22.521357603209992 62-63 22.27428068459922 27.584161347508555 27.449027094125306 22.692530873766923 64-65 22.317878634130537 27.38925344724204 27.498388455831314 22.79447946279611 66-67 22.154557175814237 27.443774343863858 27.483531136689905 22.918137343632 68-69 22.43200098997649 27.554679326366 27.45626666366674 22.557053019990775 70-71 22.42982853130311 27.334746949155843 27.467806378955462 22.76761814058559 72-73 22.272404974662198 27.500630815497352 27.468468505966786 22.758495703873663 74-75 22.372588374972356 27.551027984311094 27.35201428947802 22.72436935123853 76-77 22.33146667806609 27.496158319965264 27.304468048246548 22.8679069537221 78-79 22.46558058360859 27.52846845099633 27.377703161346307 22.62824780404877 80-81 22.41580814272075 27.40672351398457 27.44956205800955 22.72790628528513 82-83 22.347416055234433 27.399142648672477 27.55140541855219 22.70203587754091 84-85 22.334009162219296 27.395008690755247 27.38164435546387 22.889337791561594 86-87 22.536988538952713 27.436946240623495 27.352270835302008 22.673794385121788 88-89 22.23464010172887 27.47551271655857 27.384135372110414 22.905711809602145 90-91 22.29427536366382 27.31943078168247 27.4840701100764 22.902223744577313 92-93 22.504712451321733 27.435775168854637 27.311598406568827 22.747913973254807 94-95 22.29784385906201 27.21268655667076 27.482548672765468 23.006920911501762 96-97 22.41394748410651 27.204908004720913 27.40032288675881 22.980821624413768 98-99 22.30285359466537 27.3931476831979 27.371381262204945 22.932617459931787 100 22.51706315335263 27.1407288887769 27.27344105388622 23.068766903984244 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3245.0 1 1793.0 2 289.0 3 221.0 4 196.0 5 169.5 6 161.0 7 159.5 8 148.0 9 161.0 10 172.0 11 171.5 12 192.5 13 215.0 14 228.5 15 268.0 16 351.5 17 486.5 18 653.5 19 868.5 20 1275.5 21 1931.5 22 2985.5 23 4840.5 24 7396.0 25 10740.5 26 15888.0 27 23358.0 28 33655.5 29 45261.5 30 57345.5 31 70850.0 32 83935.5 33 97272.5 34 111159.5 35 123102.0 36 133740.0 37 144282.5 38 150977.5 39 153472.5 40 156767.0 41 160166.0 42 161133.0 43 161112.0 44 159906.5 45 159759.0 46 160423.0 47 156581.0 48 152093.0 49 149409.0 50 147244.0 51 143842.5 52 134097.0 53 126590.0 54 123429.5 55 118511.5 56 110140.0 57 99517.5 58 90025.0 59 82869.0 60 74966.5 61 64346.0 62 55904.5 63 48610.5 64 40650.5 65 32568.0 66 25390.5 67 18846.5 68 13763.5 69 10491.5 70 7586.5 71 5364.5 72 4080.5 73 3038.0 74 2223.5 75 1545.0 76 980.0 77 631.5 78 402.0 79 275.0 80 223.5 81 158.0 82 93.0 83 67.5 84 52.5 85 43.5 86 41.0 87 33.5 88 24.0 89 21.5 90 15.5 91 13.5 92 10.0 93 5.0 94 5.5 95 5.0 96 5.5 97 3.5 98 1.0 99 0.5 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07603229236687042 2 0.07837036404225615 3 0.07967428863045203 4 0.08196739738900342 5 0.08007895488196111 6 0.08025880654929847 7 0.07944947404628033 8 0.07958436279678335 9 0.0792471409205258 10-11 0.07903356706556268 12-13 0.07784204976945265 14-15 0.07616718111737344 16-17 0.0744473495484599 18-19 0.0739864796509079 20-21 0.07355933194098167 22-23 0.07549273736485833 24-25 0.07557142246931843 26-27 0.0747508492370917 28-29 0.07078287182646113 30-31 0.07600981090845325 32-33 0.07463844194500585 34-35 0.07190694474731964 36-37 0.06871457765208144 38-39 0.07035572411653489 40-41 0.07296357329292666 42-43 0.07802190143679001 44-45 0.07922465946210862 46-47 0.08247323020338976 48-49 0.08587917115359107 50-51 0.08682339240711223 52-53 0.08695828115761525 54-55 0.08704820699128395 56-57 0.08442911708568358 58-59 0.08345117364453668 60-61 0.08128171290727973 62-63 0.08185499009691757 64-65 0.08127047217807114 66-67 0.08062975061318177 68-69 0.08003399196512677 70-71 0.07822423456254454 72-73 0.07793197560312132 74-75 0.07735869841348347 76-77 0.07733621695506632 78-79 0.07762847591448953 80-81 0.07901108560714551 82-83 0.078482771334342 84-85 0.07743738351794358 86-87 0.07846028987592484 88-89 0.07540281153118965 90-91 0.07289612891767515 92-93 0.07276124016717213 94-95 0.073008536209761 96-97 0.07212051860228277 98-99 0.0717158523507737 100 0.07086155693092122 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4448110.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.41135333610221 #Duplication Level Percentage of deduplicated Percentage of total 1 72.01325041813905 37.743119125462954 2 15.056069877911476 15.782179964485271 3 5.320840492867605 8.366173533501726 4 2.51046359750327 5.263071785846646 5 1.3630352542850415 3.571926116094861 6 0.8196436198601224 2.5775178822102425 7 0.5621956866242147 2.0625805742986016 8 0.4199423180081951 1.7607796167927448 9 0.28538920670663087 1.3461871095910023 >10 1.5173438399613828 14.17026013181149 >50 0.09091597816808161 3.265797288808639 >100 0.04022298091407684 3.7916475364369067 >500 6.008879765811578E-4 0.1962710468146338 >1k 8.584107430978457E-5 0.1024882878444085 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.496291683434088E-5 0.0 10-11 0.0 0.0 0.0 5.6203646042926096E-5 0.0 12-13 0.0 0.0 0.0 7.868510446009653E-5 0.0 14-15 0.0 0.0 0.0 8.992583366868175E-5 0.0 16-17 0.0 0.0 0.0 1.798516673373635E-4 0.0 18-19 0.0 0.0 0.0 2.4729604258887485E-4 0.0 20-21 0.0 0.0 0.0 3.372218762575566E-4 0.0 22-23 0.0 0.0 0.0 5.845179188464315E-4 0.0 24-25 0.0 0.0 0.0 9.329805243125732E-4 0.0 26-27 0.0 0.0 0.0 0.0012702024005701298 0.0 28-29 0.0 0.0 0.0 0.002090775632796851 0.0 30-31 0.0 0.0 0.0 0.006564585857813768 0.0 32-33 0.0 0.0 0.0 0.01610796495590262 0.0 34-35 0.0 0.0 0.0 0.029237136671530155 0.0 36-37 0.0 0.0 0.0 0.0530000382184793 0.0 38-39 0.0 0.0 0.0 0.09580473504477183 0.0 40-41 0.0 0.0 0.0 0.14969279087072937 0.0 42-43 0.0 0.0 0.0 0.2076949535870291 0.0 44-45 0.0 0.0 0.0 0.2743187556063137 0.0 46-47 0.0 0.0 0.0 0.35457756215561215 0.0 48-49 0.0 0.0 0.0 0.446695338019968 0.0 50-51 0.0 0.0 0.0 0.5504585093444182 0.0 52-53 0.0 0.0 0.0 0.657020622241806 0.0 54-55 0.0 0.0 0.0 0.7695515623489527 0.0 56-57 0.0 0.0 0.0 0.901630130549829 0.0 58-59 0.0 0.0 0.0 1.051064384648761 0.0 60-61 0.0 0.0 0.0 1.2164155113070496 0.0 62-63 0.0 0.0 0.0 1.3867125588171154 0.0 64-65 0.0 0.0 0.0 1.5682053726189324 0.0 66-67 0.0 0.0 0.0 1.7689423148258474 0.0 68-69 0.0 0.0 0.0 1.9983768387022802 0.0 70-71 0.0 0.0 0.0 2.249831951098332 0.0 72-73 0.0 0.0 0.0 2.509919943526577 0.0 74-75 0.0 0.0 0.0 2.768288104385908 0.0 76-77 0.0 0.0 0.0 3.045383320106742 0.0 78-79 0.0 0.0 0.0 3.365687898905378 0.0 80-81 0.0 0.0 0.0 3.712857820512532 0.0 82-83 0.0 0.0 0.0 4.075348856030988 0.0 84-85 2.248145841717044E-5 0.0 0.0 4.434951024142839 0.0 86-87 2.248145841717044E-5 0.0 0.0 4.81202128544483 0.0 88 2.248145841717044E-5 0.0 0.0 5.118016415960936 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCAACGC 4205 0.0 32.079014 4 GTATCAA 4625 0.0 31.096737 1 GTACATG 12335 0.0 30.863913 1 ATCAACG 4375 0.0 30.832518 3 GGTATCA 2970 0.0 30.700853 1 CAACGCA 4465 0.0 30.211033 5 TACATGG 12605 0.0 29.046898 2 ACATGGG 12720 0.0 28.266983 3 AACGCAG 4805 0.0 27.975178 6 TATCAAC 5615 0.0 24.693201 2 CATGGGG 10175 0.0 22.634287 4 ACGCAGA 5955 0.0 22.414396 7 GAGTACA 8250 0.0 22.161585 1 CGCAGAG 6400 0.0 21.002533 8 ACACCGT 740 0.0 20.324436 6 TACACCG 745 0.0 20.188257 5 TATACCG 210 9.538926E-7 20.143196 5 AGTACAT 8670 0.0 19.786953 2 TACGACC 460 0.0 19.413368 4 ACCGTTC 470 0.0 17.99949 8 >>END_MODULE