FastQCFastQC Report
Wed 25 May 2016
SRR1032934_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032934_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4448110
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47800.046.591541
GTATCAA86400.040.8397251
TCAACGC93550.036.764724
ATCAACG95050.036.184533
CAACGCA96750.035.5431255
AACGCAG102350.033.598416
TATCAAC104500.033.40762
GTACATG124000.028.7970871
ACGCAGA120550.028.5239757
CGCAGAG124100.027.7459248
TACATGG128950.026.8543192
ACATGGG130050.025.541833
GCAGAGT146500.023.438989
GTGGTAT27750.023.1960621
CATGGGG101800.022.7391914
GAGTACA89300.020.3091831
TGGTATC28200.020.1880282
AGTACAT88500.019.8300232
CAGAGTA139800.019.43794310-11
AGAGTAC132550.018.71148910-11
Produced by FastQC (version 0.11.5)