FastQCFastQC Report
Wed 25 May 2016
SRR1032933_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032933_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1177616
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG37800.029.4680651
TACATGG38500.028.0777442
ACATGGG39050.027.3212093
GTGATCG1101.0181063E-425.6372919
GACCGAC950.001304329124.7377367
CATGGGG25550.024.6495514
GTATCAA26050.023.996011
TCAACGC25500.023.5920094
ATCAACG25750.023.5454833
ACCGATC1401.9308261E-523.500858
ACCGACC1602.0382322E-623.500858
AACGCAG26950.022.671476
CAACGCA26500.022.5243875
TATCAAC28450.021.476142
GGTATCA19300.021.4299161
GTCTCGC2901.2187229E-1021.0688321
TACACGG1354.0773043E-420.8887582
TCTGTCG3401.8189894E-1220.7360468
TACCTGG9550.020.6700252
GTACCTG9850.020.5176371
Produced by FastQC (version 0.11.5)