##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032933_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1177616 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.452981277428297 33.0 31.0 34.0 30.0 34.0 2 31.566070773494925 34.0 31.0 34.0 30.0 34.0 3 31.63475700058423 34.0 31.0 34.0 29.0 34.0 4 34.97154165704271 37.0 35.0 37.0 33.0 37.0 5 35.02931515876143 37.0 35.0 37.0 33.0 37.0 6 35.09889131941142 37.0 36.0 37.0 33.0 37.0 7 35.0137727408595 37.0 36.0 37.0 33.0 37.0 8 34.99992782027418 37.0 36.0 37.0 33.0 37.0 9 36.62793134604149 39.0 38.0 39.0 33.0 39.0 10-11 36.614011273624 39.0 38.0 39.0 33.0 39.0 12-13 36.555355905490416 39.0 38.0 39.0 33.0 39.0 14-15 38.01815914525618 41.0 39.0 41.0 33.0 41.0 16-17 38.04520106724094 41.0 39.0 41.0 33.0 41.0 18-19 38.065929810736264 41.0 39.0 41.0 33.0 41.0 20-21 38.11104978193231 41.0 39.0 41.0 33.0 41.0 22-23 38.109587505604544 41.0 39.0 41.0 33.0 41.0 24-25 38.14557376937813 41.0 39.0 41.0 33.0 41.0 26-27 38.12754242469532 41.0 39.0 41.0 33.0 41.0 28-29 38.11408090583008 41.0 39.0 41.0 33.0 41.0 30-31 38.07440455972066 41.0 39.0 41.0 33.0 41.0 32-33 38.01946050325403 41.0 39.0 41.0 33.0 41.0 34-35 37.94852991127838 41.0 38.5 41.0 33.0 41.0 36-37 37.85620991902284 41.0 38.0 41.0 32.0 41.0 38-39 37.755973084604825 41.0 38.0 41.0 32.0 41.0 40-41 37.6679341992636 40.5 38.0 41.0 32.0 41.0 42-43 37.55248782285567 40.0 38.0 41.0 31.5 41.0 44-45 37.41499181396992 40.0 38.0 41.0 31.0 41.0 46-47 37.2891447636581 40.0 38.0 41.0 31.0 41.0 48-49 37.1541236701947 40.0 37.5 41.0 31.0 41.0 50-51 37.015550485047754 40.0 37.0 41.0 30.0 41.0 52-53 36.85080111003927 40.0 37.0 41.0 30.0 41.0 54-55 36.68345241572805 40.0 36.0 41.0 30.0 41.0 56-57 36.50724939199196 40.0 36.0 41.0 30.0 41.0 58-59 36.303623592070764 39.5 35.0 41.0 30.0 41.0 60-61 36.05635623157294 39.0 35.0 41.0 30.0 41.0 62-63 35.7972416305485 39.0 35.0 41.0 29.5 41.0 64-65 35.51659878941862 38.0 35.0 40.5 29.0 41.0 66-67 35.21249668822435 37.5 35.0 40.0 29.0 41.0 68-69 34.895836163910815 37.0 34.5 40.0 28.5 41.0 70-71 34.55510752231628 37.0 34.0 39.5 28.0 41.0 72-73 34.260361187347996 36.0 34.0 39.0 28.0 41.0 74-75 33.98995428051249 36.0 34.0 39.0 28.0 41.0 76-77 33.67440659773645 35.0 34.0 38.5 27.5 40.0 78-79 33.314669213054174 35.0 34.0 37.0 27.0 39.0 80-81 32.99295738169319 35.0 34.0 37.0 27.0 39.0 82-83 32.686046639991304 35.0 34.0 36.5 26.0 39.0 84-85 32.5251924226573 35.0 34.0 36.0 26.5 37.5 86-87 32.29827082852135 35.0 34.0 36.0 26.0 37.0 88-89 32.06708511093599 35.0 33.0 35.5 26.0 37.0 90-91 31.87039238597302 35.0 33.0 35.0 26.0 36.0 92-93 31.678541222266002 35.0 33.0 35.0 25.0 36.0 94-95 31.49524675276151 35.0 33.0 35.0 25.0 36.0 96-97 31.325775974511217 35.0 33.0 35.0 24.5 36.0 98-99 31.149527520006522 35.0 33.0 35.0 24.0 35.5 100 31.026760845640684 35.0 33.0 35.0 23.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3153366194270859 1101 2 0.4206388632966558 1101 3 0.3727925353735273 1101 4 0.3582500320808677 1101 5 0.42015465392547213 1101 6 0.4257213134154725 1101 7 0.49496131552682243 1101 8 0.49522349553298994 1101 9 0.48618479946350135 1101 10-11 0.4185350597790958 1101 12-13 0.25174841178264273 1101 14-15 0.3276747416875523 1101 16-17 0.2936081413817746 1101 18-19 0.34013719253505315 1101 20-21 0.3121242889587066 1101 22-23 0.3076652926153898 1101 24-25 0.40175622502385266 1101 26-27 0.4344453140353721 1101 28-29 0.47750649842951987 1101 30-31 0.4191059452865815 1101 32-33 0.4483777086024787 1101 34-35 0.40714078443068047 1101 36-37 0.48146152031160483 1101 38-39 0.5545440514953057 1101 40-41 0.4508812485425082 1101 42-43 0.4355940261340763 1101 44-45 0.38254752900751043 1101 46-47 0.35707674855432714 1101 48-49 0.40499184673504374 1101 50-51 0.4003437987603746 1101 52-53 0.38088412003003214 1101 54-55 0.3753582499571664 1101 56-57 0.37239006208695713 1101 58-59 0.33004978551831954 1101 60-61 0.3552687517383575 1101 62-63 0.327581255805498 1101 64-65 0.353177464553589 1101 66-67 0.45612383130220024 1101 68-69 0.3897839709291375 1101 70-71 0.4607464027307344 1101 72-73 0.3861444237905829 1101 74-75 0.38735000052955826 1101 76-77 0.5889880196528381 1101 78-79 0.4844110019588044 1101 80-81 0.4462109087387134 1101 82-83 0.3770963995513412 1101 84-85 0.33997037739226954 1101 86-87 0.19178560016591462 1101 88-89 0.2545699369040335 1101 90-91 0.3180631130410312 1101 92-93 0.432935319699979 1101 94-95 0.3777444108467698 1101 96-97 0.35482531844967014 1101 98-99 0.3411459053867283 1101 100 0.4048442498213589 1102 1 0.2502397069069602 1102 2 0.2885392480613831 1102 3 0.26654214209087357 1102 4 0.2663485085230448 1102 5 0.19138999887081098 1102 6 0.12320637634837084 1102 7 0.18085394053698423 1102 8 0.21166171293810265 1102 9 0.2536358060368826 1102 10-11 0.29959621695862637 1102 12-13 0.2740306559724246 1102 14-15 0.278363235733444 1102 16-17 0.24046529498224345 1102 18-19 0.17756949036474623 1102 20-21 0.23765976727482752 1102 22-23 0.2237871189202778 1102 24-25 0.2529338475860712 1102 26-27 0.28590168767000534 1102 28-29 0.34242273114234933 1102 30-31 0.42208357460175705 1102 32-33 0.4377890047546771 1102 34-35 0.4025758279488443 1102 36-37 0.2874168173109908 1102 38-39 0.3933869870644813 1102 40-41 0.27621636373403646 1102 42-43 0.2612337914997269 1102 44-45 0.2526558601538085 1102 46-47 0.26240613141134617 1102 48-49 0.2594461852758201 1102 50-51 0.21319612246614383 1102 52-53 0.1629483590108265 1102 54-55 0.26073630777777623 1102 56-57 0.155860910058081 1102 58-59 0.13765648598043612 1102 60-61 0.06557137602718655 1102 62-63 0.13134241927755852 1102 64-65 0.0953488375666538 1102 66-67 0.1216635992769497 1102 68-69 0.10319659771963074 1102 70-71 0.06509637748390418 1102 72-73 0.03543509535621325 1102 74-75 0.020627928969766174 1102 76-77 -0.015234761734191693 1102 78-79 0.010639118177088847 1102 80-81 0.008158682336819822 1102 82-83 0.05377374048482153 1102 84-85 0.08504839699731548 1102 86-87 0.08297215504055089 1102 88-89 0.15139827535579542 1102 90-91 0.08777866032933801 1102 92-93 0.04071682950633715 1102 94-95 0.034932198134953296 1102 96-97 0.18604854645231228 1102 98-99 0.2829523504645195 1102 100 0.13393964599703878 1103 1 0.045017319371893905 1103 2 0.030577518599169906 1103 3 -0.002470984436826029 1103 4 -0.0059637514754982135 1103 5 0.0703677317463729 1103 6 0.01736513415866625 1103 7 0.06742587041584613 1103 8 0.16309805557931156 1103 9 0.2500371197950173 1103 10-11 0.174202432181211 1103 12-13 0.0756911657795527 1103 14-15 0.09420976154767402 1103 16-17 0.21413486432631856 1103 18-19 0.18761464066802347 1103 20-21 0.10772000206819854 1103 22-23 0.07933046638012797 1103 24-25 0.037732447167663 1103 26-27 0.12428928316397503 1103 28-29 0.12834953562540363 1103 30-31 0.18737476716076173 1103 32-33 0.17333088576923217 1103 34-35 0.16749948804893222 1103 36-37 0.10616067795299955 1103 38-39 0.07494138960592522 1103 40-41 0.10501771787468783 1103 42-43 0.15201843553303007 1103 44-45 0.11804262957481626 1103 46-47 0.08549074383132194 1103 48-49 0.16000664003598786 1103 50-51 0.11379710712152757 1103 52-53 0.13404409578168242 1103 54-55 0.18626299376614952 1103 56-57 0.12183587879479774 1103 58-59 0.2253442038746769 1103 60-61 0.28001744658015326 1103 62-63 0.12112649073397108 1103 64-65 0.06147008237974205 1103 66-67 0.11605886744852256 1103 68-69 0.24844800465967154 1103 70-71 0.19291753453135385 1103 72-73 0.10681229382669244 1103 74-75 0.014735250658489463 1103 76-77 0.08094783797119476 1103 78-79 0.08603577689252973 1103 80-81 0.09132436928538112 1103 82-83 0.09460429383579338 1103 84-85 0.11845313663695123 1103 86-87 0.0840727259225389 1103 88-89 0.1390849247346182 1103 90-91 0.11243170600357999 1103 92-93 0.2089943752811685 1103 94-95 0.14380102349753088 1103 96-97 0.1392039093312043 1103 98-99 0.22067098161520704 1103 100 0.22854157978887457 1104 1 0.10875125141341258 1104 2 0.17801153525924462 1104 3 0.09649445802467937 1104 4 0.0578701070031542 1104 5 0.12012897134445666 1104 6 4.2441392292857927E-4 1104 7 0.11159122953445433 1104 8 0.14803042451622161 1104 9 0.20226165237315996 1104 10-11 0.13834585000554256 1104 12-13 0.1487490848433879 1104 14-15 0.1912404580987257 1104 16-17 0.19791063790576402 1104 18-19 0.08599476933925132 1104 20-21 0.15435907202946453 1104 22-23 0.1306724571698865 1104 24-25 0.16620489085804735 1104 26-27 0.23835105896306175 1104 28-29 0.11927323860150807 1104 30-31 0.12596253021179393 1104 32-33 0.17633718326085557 1104 34-35 0.17548722473639344 1104 36-37 0.18596682066496584 1104 38-39 0.20211372656638105 1104 40-41 0.24156932031203837 1104 42-43 0.27304210746815727 1104 44-45 0.008935716791455661 1104 46-47 -0.04122023064278579 1104 48-49 -0.03155446604337442 1104 50-51 -0.01833540809067813 1104 52-53 0.045263708577365946 1104 54-55 0.06417946376908645 1104 56-57 0.11378861146393149 1104 58-59 0.09063167309946607 1104 60-61 0.08277435319783422 1104 62-63 0.07998124872064238 1104 64-65 0.05903995647243221 1104 66-67 0.10562080695688536 1104 68-69 0.05758532059304855 1104 70-71 0.08138368498229909 1104 72-73 0.13570272767751135 1104 74-75 0.048295650874500495 1104 76-77 0.08442825451560765 1104 78-79 0.06130929893800641 1104 80-81 -0.015024999051966859 1104 82-83 0.05827040346571977 1104 84-85 0.040781592709713266 1104 86-87 0.009120456741982252 1104 88-89 -0.04227199123788239 1104 90-91 -0.06154978847070325 1104 92-93 0.028918431188948546 1104 94-95 0.10141201625605589 1104 96-97 0.0016200880592052158 1104 98-99 0.11486908258768125 1104 100 0.14634464391613733 1105 1 0.01608499997706403 1105 2 0.09638691580138925 1105 3 0.056905741600267845 1105 4 0.09154487477223938 1105 5 -0.048965353772658204 1105 6 0.04962509555844008 1105 7 0.06722530884344735 1105 8 0.07966234133860439 1105 9 0.08141524031257319 1105 10-11 0.1705760479388445 1105 12-13 0.1131400015773778 1105 14-15 0.25255784823227856 1105 16-17 0.1290841569005039 1105 18-19 0.1309460208886577 1105 20-21 0.14224441912705998 1105 22-23 0.13832287024182932 1105 24-25 0.16853901970689122 1105 26-27 0.26125656117591234 1105 28-29 0.10542828806941884 1105 30-31 0.10577298373786448 1105 32-33 0.22946764986050994 1105 34-35 0.18925149313962208 1105 36-37 0.1521276052447078 1105 38-39 0.22666884428006284 1105 40-41 0.2450180958964978 1105 42-43 0.2398827803057202 1105 44-45 0.1435900786626263 1105 46-47 0.22137645943711703 1105 48-49 0.16092071777826789 1105 50-51 0.06057531122390003 1105 52-53 0.037537752365665256 1105 54-55 0.11770768812501586 1105 56-57 0.1651934055240858 1105 58-59 0.2555654726418837 1105 60-61 0.2094843518205849 1105 62-63 0.0900557229506731 1105 64-65 0.10857107510702946 1105 66-67 0.1600159787738491 1105 68-69 0.13679821246666535 1105 70-71 0.1378077296956164 1105 72-73 0.1274911369959426 1105 74-75 0.12050050331357198 1105 76-77 0.16613715339469337 1105 78-79 0.16380746431890003 1105 80-81 0.13684292070580284 1105 82-83 0.19622810752984066 1105 84-85 0.11921233044242996 1105 86-87 0.17396946296426563 1105 88-89 0.12660495464034227 1105 90-91 0.1499781974361376 1105 92-93 0.13135184530718647 1105 94-95 0.11136675204006963 1105 96-97 0.10605822182503744 1105 98-99 0.13952492448770926 1105 100 0.05126615099663567 1106 1 0.007590903786631742 1106 2 0.05436845755256314 1106 3 -0.037303651038445906 1106 4 -0.021034327466651348 1106 5 -0.011426833577068862 1106 6 -0.00858658470821183 1106 7 0.040573657191792734 1106 8 0.0027084240705477214 1106 9 0.05143237786897714 1106 10-11 0.05907433025257802 1106 12-13 0.12228412606836514 1106 14-15 0.12258854646977824 1106 16-17 0.061880172811697776 1106 18-19 0.07228428323477942 1106 20-21 0.1604761652711062 1106 22-23 0.13384391484664349 1106 24-25 0.16397850369349953 1106 26-27 0.16730364228843797 1106 28-29 0.11860671518763866 1106 30-31 0.1034519454220515 1106 32-33 0.21105509463512817 1106 34-35 0.16906671411976504 1106 36-37 0.22048179058187856 1106 38-39 0.2689948744987589 1106 40-41 0.15981574695069867 1106 42-43 0.21991123808287227 1106 44-45 0.2528017117616983 1106 46-47 0.17793167302766477 1106 48-49 0.17669729278259183 1106 50-51 0.1357697792521435 1106 52-53 0.20285670351577068 1106 54-55 0.21712271178741815 1106 56-57 0.28037370362323344 1106 58-59 0.1827770428508373 1106 60-61 0.22033682733275128 1106 62-63 0.19667756075706677 1106 64-65 0.26976581041825654 1106 66-67 0.2253632304758142 1106 68-69 0.32792323801577794 1106 70-71 0.27429844161171957 1106 72-73 0.15303631568247766 1106 74-75 0.17583938276893463 1106 76-77 0.24951814564348496 1106 78-79 0.19323119433163072 1106 80-81 0.3466694029472066 1106 82-83 0.3788850773268635 1106 84-85 0.272794413834724 1106 86-87 0.25894039593912765 1106 88-89 0.345218856500324 1106 90-91 0.3714884839006096 1106 92-93 0.30756700025271044 1106 94-95 0.367331977736967 1106 96-97 0.3540378768727521 1106 98-99 0.27698846787402687 1106 100 0.3330050233768702 1107 1 0.18089856899374013 1107 2 0.31406446641058494 1107 3 0.20245677978343934 1107 4 0.3026319365627401 1107 5 0.3362787630810402 1107 6 0.2481229879929785 1107 7 0.15387956086082255 1107 8 0.27627528702288373 1107 9 0.21863253892388457 1107 10-11 0.21487262156063736 1107 12-13 0.2693395264408025 1107 14-15 0.3472054119385959 1107 16-17 0.4004440159844833 1107 18-19 0.34921505478845916 1107 20-21 0.4414990526836675 1107 22-23 0.3879722051481238 1107 24-25 0.3773152342788322 1107 26-27 0.35254258234581215 1107 28-29 0.3532303751604573 1107 30-31 0.3468474358845839 1107 32-33 0.3447989292518727 1107 34-35 0.3782084676050985 1107 36-37 0.44640565438981383 1107 38-39 0.2833545554087209 1107 40-41 0.27020504686512936 1107 42-43 0.2778683572131726 1107 44-45 0.2138257945036557 1107 46-47 0.2911724627000254 1107 48-49 0.26328632594983503 1107 50-51 0.2673941060929508 1107 52-53 0.3586889673438449 1107 54-55 0.3136043540441946 1107 56-57 0.4524841520604923 1107 58-59 0.4027346401129037 1107 60-61 0.47543018885068733 1107 62-63 0.3654363684474333 1107 64-65 0.23651394822085337 1107 66-67 0.2730320235705861 1107 68-69 0.32810568303062126 1107 70-71 0.3844257127375954 1107 72-73 0.29121891168630754 1107 74-75 0.31436516413689475 1107 76-77 0.24048302812372668 1107 78-79 0.2528863103414949 1107 80-81 0.3199339733835629 1107 82-83 0.19887255782412439 1107 84-85 0.2336697095214717 1107 86-87 0.18738703391788647 1107 88-89 0.20328670088101575 1107 90-91 0.24719530105295817 1107 92-93 0.3729232084161822 1107 94-95 0.2683665667953008 1107 96-97 0.24775421891311566 1107 98-99 0.24923386271020576 1107 100 0.3047518400259186 1108 1 -0.006216167249352367 1108 2 0.027721328495797337 1108 3 0.02374151241635758 1108 4 -0.0038957485810868775 1108 5 0.04434107836554091 1108 6 -0.054764539771710474 1108 7 0.12478889652751235 1108 8 0.011401673727654327 1108 9 -0.0469710639079608 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-0.29446950498272173 2215 1 -0.16068088913304734 2215 2 -0.2466720195442491 2215 3 -0.24070423472072022 2215 4 -0.23222262844368657 2215 5 -0.16182494295405547 2215 6 -0.08355010456610046 2215 7 -0.1560923750376233 2215 8 -0.05737278389798206 2215 9 -0.20329417967448649 2215 10-11 -0.13870751849758278 2215 12-13 -0.14300343261708548 2215 14-15 -0.15562329212938408 2215 16-17 -0.22137385586486857 2215 18-19 -0.13429007281141025 2215 20-21 -0.10401200247410003 2215 22-23 -0.06253662061839549 2215 24-25 -0.0775723781742883 2215 26-27 -0.1631287267002719 2215 28-29 -0.2004533660203336 2215 30-31 -0.19178660298550199 2215 32-33 -0.2811944612587922 2215 34-35 -0.2135703243271294 2215 36-37 -0.17123801020482432 2215 38-39 -0.203367452617087 2215 40-41 -0.09839069893180863 2215 42-43 -0.09470619625287213 2215 44-45 -0.09788506656592233 2215 46-47 -0.13212336440412997 2215 48-49 -0.09305432503239786 2215 50-51 -0.10319497775839181 2215 52-53 -0.12639640522677098 2215 54-55 -0.1063998424569661 2215 56-57 -0.013438947055618655 2215 58-59 -0.06908978876951011 2215 60-61 -0.011740320237358048 2215 62-63 -0.13391579668488873 2215 64-65 -0.03202409568503839 2215 66-67 -0.15197387775918259 2215 68-69 -0.1987833981979037 2215 70-71 -0.09252762741505194 2215 72-73 -0.13392599874462263 2215 74-75 -0.013287155741402046 2215 76-77 -0.08368750719244389 2215 78-79 -0.07772585426626222 2215 80-81 -0.133115046995691 2215 82-83 -0.18052917847823835 2215 84-85 -0.1472847322127322 2215 86-87 -0.08540914726604854 2215 88-89 -0.1424984382592278 2215 90-91 -0.08121501184866275 2215 92-93 -0.11213069039568424 2215 94-95 -0.08328580553926557 2215 96-97 -0.060807954874487535 2215 98-99 -0.10865818229726898 2215 100 -0.05193502230261515 2216 1 -0.2897188368932717 2216 2 -0.3695029276447883 2216 3 -0.33460094833574416 2216 4 -0.29025313190645363 2216 5 -0.22052854578651448 2216 6 -0.2973259456160804 2216 7 -0.27777162178884396 2216 8 -0.36741999163798056 2216 9 -0.3660182219020669 2216 10-11 -0.4083006759691159 2216 12-13 -0.27659939142704104 2216 14-15 -0.416269026572877 2216 16-17 -0.44076404599273644 2216 18-19 -0.4941913775088409 2216 20-21 -0.5700794939792999 2216 22-23 -0.5073262608539224 2216 24-25 -0.5689336211385196 2216 26-27 -0.5652414027566053 2216 28-29 -0.6245649786056049 2216 30-31 -0.5601412057401518 2216 32-33 -0.4907345748686254 2216 34-35 -0.5967830444786131 2216 36-37 -0.6324887559314902 2216 38-39 -0.5424785464359871 2216 40-41 -0.46181831805353823 2216 42-43 -0.4971262413855513 2216 44-45 -0.4920249519185589 2216 46-47 -0.533769150724261 2216 48-49 -0.3898716933305906 2216 50-51 -0.3309301734630026 2216 52-53 -0.3470925897000967 2216 54-55 -0.4658867825135147 2216 56-57 -0.48439970237945573 2216 58-59 -0.44926902255158296 2216 60-61 -0.4269803793767224 2216 62-63 -0.43933949912049286 2216 64-65 -0.41940315827419994 2216 66-67 -0.431288068613334 2216 68-69 -0.4328527313237345 2216 70-71 -0.432403253176993 2216 72-73 -0.4458076940731317 2216 74-75 -0.41760264351148635 2216 76-77 -0.4803375733351629 2216 78-79 -0.40013461623031077 2216 80-81 -0.42957835724392623 2216 82-83 -0.4554701688032452 2216 84-85 -0.4545850720047042 2216 86-87 -0.45294085686062857 2216 88-89 -0.520118856903693 2216 90-91 -0.5204142959510669 2216 92-93 -0.5121812701171073 2216 94-95 -0.4645097051060958 2216 96-97 -0.5307986170797605 2216 98-99 -0.5188078463960437 2216 100 -0.5779719065917739 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19282.0 3 3152.0 4 1913.0 5 1740.0 6 1274.0 7 1359.0 8 1673.0 9 1672.0 10 1884.0 11 2041.0 12 2150.0 13 2322.0 14 2684.0 15 2819.0 16 2927.0 17 2774.0 18 2678.0 19 2427.0 20 2533.0 21 2659.0 22 3055.0 23 4393.0 24 7083.0 25 9545.0 26 9503.0 27 7908.0 28 8270.0 29 10289.0 30 12626.0 31 14724.0 32 19098.0 33 25980.0 34 36986.0 35 57932.0 36 109764.0 37 233212.0 38 373121.0 39 171785.0 40 379.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.31304330090701 19.418654751049317 12.86806790746134 23.400234040582333 2 15.702428067718369 19.775694167657434 40.79112974708018 23.730748017544023 3 18.62396829065989 25.32693458248916 29.305729301225135 26.743367825625825 4 12.21474536572763 16.700717619583433 38.82250558356308 32.26203143112586 5 12.57793112825917 37.883791685080055 35.014362443485055 14.523914743175714 6 30.527782003418096 38.06849616590421 18.08913051053006 13.314591320147636 7 27.679167835198736 32.47830779559612 21.955740254442546 17.886784114762598 8 24.882211767265407 36.89754633343758 20.075839984770813 18.144401914526195 9 24.686513915765197 17.90144702881905 21.063545360830876 36.34849369458488 10-11 23.79235500229032 27.32803259309368 28.26923125952353 20.610381145092465 12-13 24.56454491374182 24.5901249256395 28.425299566584517 22.420030594034163 14-15 21.838304873442112 26.622982890072315 25.514685167897568 26.02402706858801 16-17 21.588894648729955 30.034842317269046 25.863749543225715 22.512513490775284 18-19 21.470582236657606 28.887866287992743 27.95613806981673 21.685413405532923 20-21 22.64281380285568 28.275569326156457 27.409704081268448 21.67191278971941 22-23 22.342721851653877 28.216629090498085 27.44456842361103 21.996080634237003 24-25 22.158150554868385 28.087086100735053 27.558200949163375 22.196562395233194 26-27 21.937638015290727 27.85324372315912 27.763163317899277 22.445954943650882 28-29 22.0138463407981 28.147612156295228 27.682951597291915 22.15558990561476 30-31 22.256120749765554 28.57723772418883 27.210381107736836 21.956260418308773 32-33 21.736152391991865 28.27789077939389 27.867515743861055 22.118441084753197 34-35 21.892653146724193 27.751516776424666 27.974885182181 22.380944894670133 36-37 22.400847384893062 28.38102068581072 27.245098347463497 21.97303358183272 38-39 22.123407251237172 28.516284075550324 27.451810174981954 21.90849849823055 40-41 21.766828383671253 28.12082801145871 27.850421543458364 22.26192206141167 42-43 21.65346869539796 28.165685954571106 27.716292058441606 22.464553291589333 44-45 21.940322797904784 28.28074400825033 27.736203667860558 22.042729525984324 46-47 21.872939060789633 28.66702701232654 27.42158232538533 22.038451601498494 48-49 21.740806198084943 28.256282925307758 27.79500459578548 22.207906280821827 50-51 21.79279988679308 28.09745654133306 27.858081695112176 22.251661876761688 52-53 21.935691137962422 28.09826254055106 27.65962690899139 22.306419412495124 54-55 21.939525030755927 28.64897023421249 27.474826885035515 21.93667784999607 56-57 21.680680553495765 28.277951319466904 27.592604475236705 22.44876365180063 58-59 21.665192835352574 27.749814407738654 28.031124257790307 22.55386849911846 60-61 22.159837676479025 28.339774574272568 27.467179416357705 22.0332083328907 62-63 21.758346637272645 28.304097245032857 27.63937478779916 22.298181329895346 64-65 21.742967733820702 27.994956935509705 27.894121141479882 22.367954189189707 66-67 21.638540660117673 28.053853717570533 27.613585468810687 22.694020153501114 68-69 22.179073312118177 28.252418149624216 27.513556212869407 22.054952325388204 70-71 22.235026419279382 27.981957979854638 27.6007215108386 22.182294090027384 72-73 21.732952493292665 28.117901209912155 28.02832844819015 22.120817848605036 74-75 21.701841247826554 28.29756386918693 27.760044599227328 22.240550283759184 76-77 21.92875183563581 28.256808849124333 27.476578646796472 22.33786066844338 78-79 22.061617979743385 28.267097361948906 27.611918735584712 22.059365922723 80-81 21.902935954931092 28.075223062717612 27.726277251299624 22.295563731051672 82-83 21.722573347508654 28.14098494679581 27.909956122794988 22.22648558290055 84-85 21.86582578504404 28.147070433750613 27.65654671129424 22.3305570699111 86-87 22.03682520689321 28.223862788456472 27.63347990216658 22.10583210248374 88-89 21.68521885520455 28.14139746270477 27.618973158990777 22.554410523099907 90-91 21.739551638557504 28.020529398092968 27.69085061714389 22.549068346205633 92-93 22.020608376683498 28.3598660553242 27.469111016783877 22.15041455120843 94-95 21.773724448281737 27.990096498380506 27.71756749809115 22.518611555246608 96-97 21.804636190704418 27.992668025478356 27.776141935061716 22.426553848755507 98-99 21.743798703230027 28.117219167721814 27.720625100911818 22.41835702813634 100 22.003999058445228 27.974034162802297 27.5404770827438 22.481489696008676 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1560.0 1 1058.0 2 493.5 3 383.0 4 267.0 5 195.5 6 178.5 7 182.0 8 192.5 9 192.0 10 179.0 11 176.5 12 183.5 13 180.0 14 198.0 15 237.0 16 283.0 17 360.5 18 502.0 19 679.0 20 938.5 21 1351.5 22 1957.0 23 2882.0 24 4082.5 25 5693.0 26 7952.5 27 10581.0 28 13351.5 29 16566.5 30 19804.0 31 22971.5 32 26038.0 33 28690.5 34 31421.0 35 33423.0 36 35295.0 37 37331.0 38 38383.5 39 38568.0 40 39136.0 41 39895.5 42 39805.5 43 39707.5 44 39015.5 45 38376.0 46 38662.5 47 38638.0 48 38900.5 49 38214.5 50 36559.5 51 35309.0 52 33187.5 53 31602.5 54 30563.0 55 29375.5 56 27888.5 57 25477.0 58 23134.0 59 21484.0 60 19637.5 61 17142.0 62 14778.0 63 12569.5 64 10314.0 65 8164.5 66 6580.0 67 5181.5 68 3814.5 69 2725.0 70 1943.0 71 1434.0 72 1081.0 73 839.5 74 643.5 75 477.5 76 330.5 77 235.0 78 163.5 79 107.5 80 68.0 81 59.5 82 49.5 83 38.0 84 28.0 85 19.0 86 17.5 87 12.5 88 9.5 89 10.5 90 7.5 91 4.0 92 5.5 93 4.0 94 1.5 95 1.5 96 1.0 97 1.0 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07617084007010774 2 0.07803902120895097 3 0.07880327712938684 4 0.0805016236192443 5 0.07948261572532982 6 0.0795675330498227 7 0.07863344248040108 8 0.07880327712938684 9 0.0788881944538797 10-11 0.07854852515590821 12-13 0.07778426923547234 14-15 0.07655296803032569 16-17 0.07523674950068614 18-19 0.07455741090474313 20-21 0.07421774160677164 22-23 0.076001005421122 24-25 0.07604346408336843 26-27 0.07553396013641119 28-29 0.07158530454749255 30-31 0.07655296803032569 32-33 0.07536412548742544 34-35 0.07307135772611785 36-37 0.0695472887596636 38-39 0.07111825926278176 40-41 0.07379315498430727 42-43 0.07812393853344383 44-45 0.07944015706308338 46-47 0.08219997010910178 48-49 0.08445027920816293 50-51 0.08572403907555604 52-53 0.0860212497112811 54-55 0.08606370837352753 56-57 0.08368602328772706 58-59 0.08300668469178409 60-61 0.08096866890395511 62-63 0.08147817285091236 64-65 0.08071391693047648 66-67 0.08041670629475144 68-69 0.07994966101004061 70-71 0.07799656254670453 72-73 0.07803902120895097 74-75 0.07761443458648659 76-77 0.07744459993750086 78-79 0.07761443458648659 80-81 0.0788881944538797 82-83 0.07837869050692246 84-85 0.07765689324873304 86-87 0.07829377318242958 88-89 0.07561887746090407 90-91 0.07341102702408935 92-93 0.07315627505061073 94-95 0.07307135772611785 96-97 0.07239201913017486 98-99 0.07184005652097118 100 0.07124563524952106 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1177616.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.97851525867901 #Duplication Level Percentage of deduplicated Percentage of total 1 84.966207856579 64.5560632010318 2 10.245301192362932 15.568455459474187 3 2.35659850293155 5.371525659406948 4 0.8823878521551951 2.68170075556186 5 0.45805048791049596 1.7400997992476492 6 0.28230951976160623 1.2869674892926528 7 0.17809310225007383 0.9471874640740903 8 0.12749706092809537 0.7749629911329617 9 0.08352887003638886 0.5711759573939987 >10 0.402202405603939 5.141462595626045 >50 0.013573368154298451 0.6803767514507597 >100 0.003914278094209995 0.47209998088888117 >500 3.3550323215976447E-4 0.20792189541811895 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 4.245866224643687E-5 0.0 8.491732449287373E-5 0.0 16-17 0.0 8.491732449287373E-5 0.0 8.491732449287373E-5 0.0 18-19 0.0 8.491732449287373E-5 0.0 8.491732449287373E-5 0.0 20-21 0.0 8.491732449287373E-5 0.0 1.273759867393106E-4 0.0 22-23 0.0 8.491732449287373E-5 0.0 2.1229331123218435E-4 0.0 24-25 0.0 8.491732449287373E-5 0.0 3.3966929797149493E-4 0.0 26-27 0.0 8.491732449287373E-5 0.0 3.821279602179318E-4 0.0 28-29 0.0 8.491732449287373E-5 0.0 0.0013586771918859797 0.0 30-31 0.0 8.491732449287373E-5 0.0 0.004415700873629434 0.0 32-33 0.0 8.491732449287373E-5 0.0 0.010487289574869907 0.0 34-35 0.0 8.491732449287373E-5 0.0 0.017790179481257048 0.0 36-37 0.0 8.491732449287373E-5 0.0 0.032056289996059834 0.0 38-39 0.0 8.491732449287373E-5 0.0 0.05901754052254725 0.0 40-41 0.0 8.491732449287373E-5 0.0 0.10037227755057676 0.0 42-43 0.0 8.491732449287373E-5 0.0 0.143255526419478 0.0 44-45 0.0 8.491732449287373E-5 0.0 0.19008743087729785 0.0 46-47 0.0 8.491732449287373E-5 0.0 0.24400993193027268 0.0 48-49 0.0 8.491732449287373E-5 0.0 0.3045984429559381 0.0 50-51 0.0 8.491732449287373E-5 0.0 0.37724521405959155 0.0 52-53 0.0 8.491732449287373E-5 0.0 0.4560909498512249 0.0 54-55 0.0 8.491732449287373E-5 0.0 0.5370596187551799 0.0 56-57 0.0 8.491732449287373E-5 0.0 0.6284306599095121 0.0 58-59 0.0 8.491732449287373E-5 0.0 0.7346622328500971 0.0 60-61 0.0 8.491732449287373E-5 0.0 0.8539286151003382 0.0 62-63 0.0 8.491732449287373E-5 0.0 0.9777805328731947 0.0 64-65 0.0 8.491732449287373E-5 0.0 1.1123320335321532 0.0 66-67 0.0 8.491732449287373E-5 0.0 1.2613619380171466 0.0 68-69 0.0 8.491732449287373E-5 0.0 1.4280121873344114 0.0 70-71 0.0 8.491732449287373E-5 0.0 1.607102824689882 0.0 72-73 0.0 8.491732449287373E-5 0.0 1.808951305009443 0.0 74-75 0.0 8.491732449287373E-5 0.0 1.998868901237755 0.0 76-77 0.0 8.491732449287373E-5 0.0 2.191631227836578 0.0 78-79 0.0 8.491732449287373E-5 0.0 2.412883316802761 0.0 80-81 0.0 8.491732449287373E-5 0.0 2.6613938669311556 0.0 82-83 0.0 8.491732449287373E-5 0.0 2.93470027581147 0.0 84-85 0.0 8.491732449287373E-5 0.0 3.1935707395279955 0.0 86-87 0.0 8.491732449287373E-5 0.0 3.4593619651906904 0.0 88 0.0 8.491732449287373E-5 0.0 3.673438540237225 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 3780 0.0 29.468065 1 TACATGG 3850 0.0 28.077744 2 ACATGGG 3905 0.0 27.321209 3 GTGATCG 110 1.0181063E-4 25.637291 9 GACCGAC 95 0.0013043291 24.737736 7 CATGGGG 2555 0.0 24.649551 4 GTATCAA 2605 0.0 23.99601 1 TCAACGC 2550 0.0 23.592009 4 ATCAACG 2575 0.0 23.545483 3 ACCGATC 140 1.9308261E-5 23.50085 8 ACCGACC 160 2.0382322E-6 23.50085 8 AACGCAG 2695 0.0 22.67147 6 CAACGCA 2650 0.0 22.524387 5 TATCAAC 2845 0.0 21.47614 2 GGTATCA 1930 0.0 21.429916 1 GTCTCGC 290 1.2187229E-10 21.068832 1 TACACGG 135 4.0773043E-4 20.888758 2 TCTGTCG 340 1.8189894E-12 20.736046 8 TACCTGG 955 0.0 20.670025 2 GTACCTG 985 0.0 20.517637 1 >>END_MODULE