FastQCFastQC Report
Wed 25 May 2016
SRR1032933_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032933_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1177616
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34300.29126642301055694No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28260.2399763590168612No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15170.12881958125568946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG1750.042.9904332
TAACGCA1550.039.4367874
TACACCG1700.038.7197075
GGTATCA27600.036.083671
ATAACGC1850.035.5832983
GTATCAA44750.035.481961
TCAACGC52950.029.7487494
ATCAACG53450.029.4704653
TATCAAC54500.029.0752352
CAACGCA54350.028.9799885
AACGCAG55900.028.3446456
GTACATG36600.025.6704141
ACACCGT2208.731149E-1125.6455216
TACATGG35400.024.9714892
ACGCAGA63100.024.9613697
ACATGGG35250.024.6775743
CGCAGAG64700.024.2714188
GTCTCGC3750.023.8016051
TACCTGG8100.023.8006442
GTACCTG8600.022.9421861
Produced by FastQC (version 0.11.5)