##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1032933_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1177616
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.83400191573484	34.0	31.0	34.0	31.0	34.0
2	32.87886798413065	34.0	31.0	34.0	31.0	34.0
3	32.94794398173938	34.0	31.0	34.0	31.0	34.0
4	36.36500183421421	37.0	37.0	37.0	35.0	37.0
5	36.34954942867624	37.0	37.0	37.0	35.0	37.0
6	36.41461817774215	37.0	37.0	37.0	35.0	37.0
7	36.38159722693985	37.0	37.0	37.0	35.0	37.0
8	36.321101275797886	37.0	37.0	37.0	35.0	37.0
9	38.157482574965016	39.0	39.0	39.0	37.0	39.0
10-11	38.16966693726988	39.0	39.0	39.0	37.0	39.0
12-13	38.162263420333964	39.0	39.0	39.0	37.0	39.0
14-15	39.71258372848195	41.0	40.0	41.0	37.0	41.0
16-17	39.725706427222455	41.0	40.0	41.0	37.0	41.0
18-19	39.75229616445429	41.0	40.0	41.0	37.0	41.0
20-21	39.763250923900486	41.0	40.0	41.0	37.0	41.0
22-23	39.71976094074809	41.0	40.0	41.0	37.0	41.0
24-25	39.6952699351911	41.0	40.0	41.0	37.0	41.0
26-27	39.61011526677626	41.0	40.0	41.0	37.0	41.0
28-29	39.54281404124944	41.0	40.0	41.0	37.0	41.0
30-31	39.42183190445782	41.0	39.0	41.0	36.0	41.0
32-33	39.2721362481488	41.0	39.0	41.0	35.0	41.0
34-35	39.166723278216324	41.0	39.0	41.0	35.0	41.0
36-37	39.06576761864649	41.0	39.0	41.0	35.0	41.0
38-39	38.95513011032459	41.0	39.0	41.0	35.0	41.0
40-41	38.842525916767435	41.0	39.0	41.0	35.0	41.0
42-43	38.69019400211954	41.0	39.0	41.0	35.0	41.0
44-45	38.500066660099726	41.0	38.5	41.0	35.0	41.0
46-47	38.24966500115488	41.0	38.0	41.0	34.5	41.0
48-49	38.01811583742069	41.0	38.0	41.0	34.0	41.0
50-51	37.85005086547737	40.0	38.0	41.0	34.0	41.0
52-53	37.69822548267007	40.0	38.0	41.0	33.0	41.0
54-55	37.543441580277445	40.0	37.0	41.0	33.0	41.0
56-57	37.33525954131058	40.0	37.0	41.0	33.0	41.0
58-59	37.16562444803739	40.0	36.0	41.0	33.0	41.0
60-61	36.96740745709977	40.0	36.0	41.0	33.0	41.0
62-63	36.72495703183381	39.0	35.0	41.0	32.0	41.0
64-65	36.433713111914244	39.0	35.0	41.0	32.0	41.0
66-67	36.15568147851252	38.5	35.0	41.0	31.0	41.0
68-69	35.85499602586921	37.5	35.0	40.0	31.0	41.0
70-71	35.53287149631119	37.0	35.0	40.0	31.0	41.0
72-73	35.19648637586446	36.5	35.0	39.0	31.0	41.0
74-75	34.86358286572193	36.0	35.0	39.0	30.5	41.0
76-77	34.52603862379587	35.5	35.0	39.0	30.0	40.5
78-79	34.19043219521474	35.0	35.0	37.0	30.0	39.5
80-81	33.860919433839214	35.0	34.0	37.0	30.0	39.0
82-83	33.558315698835614	35.0	34.0	36.5	29.5	39.0
84-85	33.28524578470402	35.0	34.0	36.0	29.0	37.5
86-87	33.14230827366476	35.0	34.0	36.0	29.0	37.0
88-89	32.954640561948885	35.0	34.0	35.5	29.0	37.0
90-91	32.779794941644816	35.0	34.0	35.0	29.0	36.0
92-93	32.61338246083613	35.0	34.0	35.0	29.0	36.0
94-95	32.5100720438581	35.0	34.0	35.0	29.0	36.0
96-97	32.44705829404492	35.0	34.0	35.0	29.0	36.0
98-99	32.33118350973492	35.0	34.0	35.0	29.0	36.0
100	32.24966033070203	35.0	34.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.12667201084674673
1101	2	0.22256594911551986
1101	3	0.22174645926948244
1101	4	0.11492986862747756
1101	5	0.1301326217414882
1101	6	0.12356945125296193
1101	7	0.09958553575095408
1101	8	-0.01829312150353246
1101	9	0.07686577394147776
1101	10-11	0.1714846965424499
1101	12-13	0.20625567489118168
1101	14-15	0.29143860802798827
1101	16-17	0.2779003765443022
1101	18-19	0.2820329539684252
1101	20-21	0.225473506683052
1101	22-23	0.28148510609106125
1101	24-25	0.321008479247638
1101	26-27	0.29063214303032936
1101	28-29	0.3399561809508924
1101	30-31	0.3507204077505719
1101	32-33	0.368682412576554
1101	34-35	0.4493979823481311
1101	36-37	0.434218024617266
1101	38-39	0.45453773828498356
1101	40-41	0.5398288004822334
1101	42-43	0.3823553340462311
1101	44-45	0.3238343438279472
1101	46-47	0.3333775579074967
1101	48-49	0.39684750362142296
1101	50-51	0.4138578619119926
1101	52-53	0.36199450742874717
1101	54-55	0.45245009426260197
1101	56-57	0.4303403813937976
1101	58-59	0.39876389553911906
1101	60-61	0.37322984134119963
1101	62-63	0.39974168232568985
1101	64-65	0.46097134035393594
1101	66-67	0.4541196856286831
1101	68-69	0.43299428343445356
1101	70-71	0.4000935010452906
1101	72-73	0.40986246820592953
1101	74-75	0.4434911717971275
1101	76-77	0.45598406884114695
1101	78-79	0.3587975253544897
1101	80-81	0.3693403366425443
1101	82-83	0.3543637771186283
1101	84-85	0.3920108393870976
1101	86-87	0.4185786186613214
1101	88-89	0.44238777711198196
1101	90-91	0.5006762748410623
1101	92-93	0.4584032380919183
1101	94-95	0.396438134033815
1101	96-97	0.3918422710969338
1101	98-99	0.4366039885460751
1101	100	0.558951286504417
1102	1	0.15110810107231032
1102	2	0.17398345491308476
1102	3	0.10288915065808624
1102	4	0.10263182406720972
1102	5	0.13797180021430933
1102	6	0.13488793423321965
1102	7	0.11955853929471516
1102	8	0.008388324917170564
1102	9	0.08595501572321496
1102	10-11	0.08792114897174486
1102	12-13	0.12504414944559983
1102	14-15	0.17353093314574153
1102	16-17	0.14560200968410442
1102	18-19	0.210938001338846
1102	20-21	0.21391042107557467
1102	22-23	0.1957481442743969
1102	24-25	0.21552047878106606
1102	26-27	0.2092166861394773
1102	28-29	0.1447170497487349
1102	30-31	0.1138963359360119
1102	32-33	0.22457309611331766
1102	34-35	0.1937786446591403
1102	36-37	0.19942482606669643
1102	38-39	0.2060953294459864
1102	40-41	0.0785646480619917
1102	42-43	-0.04357054924742698
1102	44-45	-0.00840911693664026
1102	46-47	-0.11354635782181077
1102	48-49	-0.07757358430967543
1102	50-51	-0.16738877120479145
1102	52-53	-0.16596904044891403
1102	54-55	-0.12828853277038377
1102	56-57	-0.2509364481571126
1102	58-59	-0.24445295706391335
1102	60-61	-0.21455545165246548
1102	62-63	-0.1857382069259117
1102	64-65	-0.15525788089268389
1102	66-67	-0.07676086848164232
1102	68-69	-0.11356731345427562
1102	70-71	-0.12168495765504161
1102	72-73	-0.11257363583249003
1102	74-75	-0.016155533760397134
1102	76-77	-0.08479013396532054
1102	78-79	-0.056698693760345975
1102	80-81	-0.09388696768686344
1102	82-83	-0.07014302724684995
1102	84-85	-0.10638092231967278
1102	86-87	-0.07239429740103276
1102	88-89	-0.05058378222113902
1102	90-91	-0.010147823064450279
1102	92-93	0.028647932804815923
1102	94-95	-0.06461019253889333
1102	96-97	-0.02356090309697123
1102	98-99	0.10885718737290517
1102	100	0.12177867033447143
1103	1	0.12583734294956628
1103	2	0.12645699441830516
1103	3	0.14132880864472241
1103	4	0.06664127711258772
1103	5	0.11601565061330632
1103	6	0.09011713815975497
1103	7	0.06782065423307415
1103	8	0.029006987975940035
1103	9	0.06703750290287047
1103	10-11	0.07033154290022026
1103	12-13	0.15018815953238374
1103	14-15	0.11402530943855282
1103	16-17	0.08183485172141047
1103	18-19	0.12334313375001216
1103	20-21	0.10713275407951528
1103	22-23	0.06981310116901085
1103	24-25	0.10221012958285769
1103	26-27	0.15822418269627292
1103	28-29	0.12458585079336615
1103	30-31	0.1075515650662453
1103	32-33	0.12656425617471712
1103	34-35	0.14627668632829938
1103	36-37	0.17023224790110447
1103	38-39	0.14887172306643492
1103	40-41	0.17863701234764306
1103	42-43	0.15496827433920402
1103	44-45	0.18220205914511922
1103	46-47	0.24256275012993456
1103	48-49	0.03219150441763219
1103	50-51	0.13104003213900484
1103	52-53	0.11822511101351552
1103	54-55	0.13325069685210877
1103	56-57	0.17746767616502268
1103	58-59	0.1610882533650937
1103	60-61	0.19824816331942685
1103	62-63	0.1812837595403849
1103	64-65	0.21674502546925822
1103	66-67	0.12415669150075814
1103	68-69	0.17145143714906652
1103	70-71	0.14434922157404628
1103	72-73	0.19092048327971867
1103	74-75	0.11876351330070634
1103	76-77	0.21087784660686282
1103	78-79	0.21073671448254316
1103	80-81	0.09583052420531146
1103	82-83	0.09133820778011881
1103	84-85	0.12169719426346148
1103	86-87	0.2471459756820309
1103	88-89	0.08119825095000266
1103	90-91	0.09601728983341218
1103	92-93	0.10806552557959037
1103	94-95	0.1940628365844077
1103	96-97	0.11577756058419197
1103	98-99	0.16518084585179338
1103	100	0.09979120217807491
1104	1	0.1829102212136533
1104	2	0.17665833555865618
1104	3	0.17135989214495595
1104	4	0.1574212561067725
1104	5	0.1588904704268046
1104	6	0.14090740745087516
1104	7	0.11273014639416346
1104	8	0.1551417908657271
1104	9	0.22042033171400277
1104	10-11	0.16104561296778286
1104	12-13	0.1300359736947243
1104	14-15	0.2723715056430507
1104	16-17	0.2517661363501773
1104	18-19	0.252124924176627
1104	20-21	0.22956390051214015
1104	22-23	0.24569000880720893
1104	24-25	0.20595715836592632
1104	26-27	0.34681832403052937
1104	28-29	0.36388415459525447
1104	30-31	0.3818804633666417
1104	32-33	0.4066251049966496
1104	34-35	0.3562997331916691
1104	36-37	0.3793591459718968
1104	38-39	0.3189082493683486
1104	40-41	0.37690382018697477
1104	42-43	0.3544565234154149
1104	44-45	0.4071045777165523
1104	46-47	0.3927585634248203
1104	48-49	0.2585301997610614
1104	50-51	0.200706830623524
1104	52-53	0.22493697844727478
1104	54-55	0.17958042007713715
1104	56-57	0.17238099529604511
1104	58-59	0.22102604384274827
1104	60-61	0.17663853976571176
1104	62-63	0.17475452361619404
1104	64-65	0.21373553883601204
1104	66-67	0.31147730228515513
1104	68-69	0.2878699885984233
1104	70-71	0.2583599711073745
1104	72-73	0.23441612266008605
1104	74-75	0.22088407986234415
1104	76-77	0.2595909216622445
1104	78-79	0.36673145027278764
1104	80-81	0.33677548812548963
1104	82-83	0.17358829601304393
1104	84-85	0.24363083460482926
1104	86-87	0.21886998311485684
1104	88-89	0.18625495388783975
1104	90-91	0.10407412458910414
1104	92-93	0.22138954661942023
1104	94-95	0.133299058429607
1104	96-97	0.23529808377350747
1104	98-99	0.24430344921186986
1104	100	0.14069938466501242
1105	1	0.08420874097923559
1105	2	0.1397203097115991
1105	3	0.11553197016714023
1105	4	0.08054197918309569
1105	5	0.08563109742458863
1105	6	0.11821465938673015
1105	7	0.09137456329362692
1105	8	0.1294704847197039
1105	9	0.0795174729971464
1105	10-11	0.0777785725938287
1105	12-13	-0.0010940694522290073
1105	14-15	0.10384812221485618
1105	16-17	0.11445183372127588
1105	18-19	0.11916231751690987
1105	20-21	0.10664185075439292
1105	22-23	0.1425366223161788
1105	24-25	0.16867738974376323
1105	26-27	0.10804598741440685
1105	28-29	0.22140773741622866
1105	30-31	0.24153929615832936
1105	32-33	0.26025744527173345
1105	34-35	0.16849229316881065
1105	36-37	0.15272458770910902
1105	38-39	0.3319587121857026
1105	40-41	0.2783308146800323
1105	42-43	0.2679889206304935
1105	44-45	0.20706171854746458
1105	46-47	0.2914582964029506
1105	48-49	0.24571869993681617
1105	50-51	0.2364351384366401
1105	52-53	0.30817267098773016
1105	54-55	0.3801330669648664
1105	56-57	0.37362791961107433
1105	58-59	0.3835147123717775
1105	60-61	0.40848814416631996
1105	62-63	0.3538333906540956
1105	64-65	0.3506119995936601
1105	66-67	0.3969818585128948
1105	68-69	0.40758171186591596
1105	70-71	0.3959836408800399
1105	72-73	0.5321681232685407
1105	74-75	0.46031934607226077
1105	76-77	0.4696805045202197
1105	78-79	0.5047158770802227
1105	80-81	0.47894407985747733
1105	82-83	0.3769961652442717
1105	84-85	0.3900617038956682
1105	86-87	0.3955006357459183
1105	88-89	0.46074737593450976
1105	90-91	0.45414360372172524
1105	92-93	0.42373158951147616
1105	94-95	0.4500565824235423
1105	96-97	0.4343722434973998
1105	98-99	0.4795910373198353
1105	100	0.43694360587176817
1106	1	0.08297747044167636
1106	2	0.10201965093764187
1106	3	0.042967586411663206
1106	4	-0.017373016964882027
1106	5	0.05100321037523514
1106	6	0.09495907395837122
1106	7	0.06499314260592826
1106	8	0.03598531106437264
1106	9	0.09175942699302198
1106	10-11	0.13542325916522202
1106	12-13	0.07946276355879434
1106	14-15	0.15963951570257962
1106	16-17	0.10893912205673217
1106	18-19	0.18056517980328124
1106	20-21	0.19796173739495515
1106	22-23	0.21087361113745828
1106	24-25	0.2252475397366993
1106	26-27	0.17933193304590134
1106	28-29	0.18391785879415323
1106	30-31	0.18759087784472683
1106	32-33	0.17393879678030544
1106	34-35	0.16760397029096197
1106	36-37	0.1625231624618806
1106	38-39	0.23133403646286155
1106	40-41	0.235364091412265
1106	42-43	0.1797796380494887
1106	44-45	0.20583241332601432
1106	46-47	0.21784590783637725
1106	48-49	0.18793840655443006
1106	50-51	0.15206952282102293
1106	52-53	0.17427224569331656
1106	54-55	0.2268777173744354
1106	56-57	0.2314648140971869
1106	58-59	0.1513484820052895
1106	60-61	0.1508410992156186
1106	62-63	0.13326891256344453
1106	64-65	0.15606634136999276
1106	66-67	0.14215946412898006
1106	68-69	0.14629146219215983
1106	70-71	0.24323386885671283
1106	72-73	0.18512621879862934
1106	74-75	0.1093428787672579
1106	76-77	0.07392037921167116
1106	78-79	0.016311242078735688
1106	80-81	0.1288510221400756
1106	82-83	0.18401391597929972
1106	84-85	0.12092984297716214
1106	86-87	0.1463820800174176
1106	88-89	0.19403039717564496
1106	90-91	0.1935613591735148
1106	92-93	0.15916908852882017
1106	94-95	0.17160027926174592
1106	96-97	0.13707740284522885
1106	98-99	0.10321138541662833
1106	100	0.04918870437341383
1107	1	0.04216955358627672
1107	2	0.06405724719311934
1107	3	0.05236350467629336
1107	4	0.04240918143463546
1107	5	0.07570815274777942
1107	6	0.086935268029535
1107	7	0.043500490736178676
1107	8	-0.010430588074989089
1107	9	0.02713740273412668
1107	10-11	-0.011726823878717596
1107	12-13	-0.004932211024730293
1107	14-15	0.05961183438998319
1107	16-17	0.08406242972193212
1107	18-19	0.030356626277693977
1107	20-21	0.1008893507625217
1107	22-23	0.042497623417894204
1107	24-25	0.0337246271593159
1107	26-27	0.1012379172423934
1107	28-29	0.07781444392676917
1107	30-31	0.012016805168698852
1107	32-33	-0.03135714161619774
1107	34-35	0.031057088213351847
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2216	14-15	-0.4345008954267797
2216	16-17	-0.4264000845531939
2216	18-19	-0.40335454793550696
2216	20-21	-0.37901769369359073
2216	22-23	-0.4278607724173682
2216	24-25	-0.39074500667712186
2216	26-27	-0.4870112056729283
2216	28-29	-0.39821002184891796
2216	30-31	-0.4582564542940446
2216	32-33	-0.5773428716284315
2216	34-35	-0.5767031821653674
2216	36-37	-0.5086900051256009
2216	38-39	-0.5387915794845739
2216	40-41	-0.41299524859072534
2216	42-43	-0.5351300229927745
2216	44-45	-0.5189044091317996
2216	46-47	-0.34286068964508587
2216	48-49	-0.47786344502090117
2216	50-51	-0.47297247359528427
2216	52-53	-0.4378504087290551
2216	54-55	-0.3844158966816096
2216	56-57	-0.3898021711200812
2216	58-59	-0.36827628779212773
2216	60-61	-0.5113832798595936
2216	62-63	-0.42632555759732327
2216	64-65	-0.37578560726372956
2216	66-67	-0.4924421609767933
2216	68-69	-0.49439497700947044
2216	70-71	-0.4997060669354383
2216	72-73	-0.5313995265599871
2216	74-75	-0.5510445459502762
2216	76-77	-0.5307766866387098
2216	78-79	-0.6254188858392382
2216	80-81	-0.665212551101682
2216	82-83	-0.5334964120282706
2216	84-85	-0.43303866282145975
2216	86-87	-0.41634190086955414
2216	88-89	-0.4656990836864239
2216	90-91	-0.4879036002610633
2216	92-93	-0.5218028018698675
2216	94-95	-0.47506209443791647
2216	96-97	-0.4932312552603939
2216	98-99	-0.556368774854139
2216	100	-0.5626337534435208
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	6.0
3	2.0
4	4.0
5	9.0
6	31.0
7	83.0
8	161.0
9	300.0
10	581.0
11	921.0
12	1583.0
13	2408.0
14	2646.0
15	3405.0
16	4345.0
17	6297.0
18	7442.0
19	4465.0
20	2867.0
21	2561.0
22	2546.0
23	2622.0
24	2937.0
25	3674.0
26	4311.0
27	5480.0
28	7004.0
29	8772.0
30	11180.0
31	14053.0
32	18251.0
33	23646.0
34	33221.0
35	52059.0
36	98682.0
37	228892.0
38	406032.0
39	213223.0
40	914.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.300622191878304	19.31113075540225	13.20232232885053	23.18592472386892
2	16.08223435602619	19.791017399648442	40.474605344723635	23.652142899601735
3	18.783149656848508	25.235943186816733	29.694195211673772	26.286711944660983
4	12.389605249860082	16.701274502560132	38.640009715672214	32.26911053190757
5	12.281474952255031	37.465258052107195	35.23149834707726	15.021768648560519
6	30.559556240956	37.98905548346773	17.52761605195758	13.923772223618688
7	27.23236045889556	32.50770628646156	22.047961549239563	18.211971705403318
8	25.428293476060976	35.69942837511246	20.107640997120615	18.764637151705944
9	25.07179845142926	16.612913361220514	21.341081282130975	36.97420690521925
10-11	24.103357970679745	26.477816198149483	28.258022988818087	21.160802842352684
12-13	25.311095146626993	23.804446958001517	28.8070199573035	22.07743793806799
14-15	22.207776823400188	25.698392810210812	26.567947320621478	25.52588304576752
16-17	21.10560658143232	29.395533009062376	26.849669162103773	22.64919124740153
18-19	20.838603530448257	27.50403889225021	30.14977337621671	21.50758420108482
20-21	22.024539408431952	26.86198217415524	29.902362060298092	21.21111635711471
22-23	21.63906069351277	26.880721051516478	30.00946405590629	21.470754199064466
24-25	21.447125958509183	26.785395844124967	30.08750774874534	21.679970448620512
26-27	21.14704745890815	26.588750323766817	30.393955220394975	21.870246996930053
28-29	21.39118087661312	26.755323846825398	30.24142868304216	21.612066593519327
30-31	21.5957495145293	27.31009953157863	29.631059253965404	21.463091699926665
32-33	21.12678149727093	26.976882842123278	30.314319796197687	21.582015864408106
34-35	21.293009649858195	26.51969765306547	30.276950335634318	21.910342361442016
36-37	21.78782220742241	27.241860291269393	29.431859883519422	21.538457617788772
38-39	21.676158663599324	27.284705538546227	29.660225021492913	21.37891077636153
40-41	21.271684158430563	26.999594301529545	29.902058866635656	21.826662673404236
42-43	21.309510455756786	27.081846209278293	29.569507328547346	22.03913600641758
44-45	21.573207266148884	27.281383043839007	29.41697044301473	21.728439246997375
46-47	21.664297483281103	27.702739467477855	28.89193633131386	21.74102671792718
48-49	21.632423172647144	27.512385680856443	28.94914591405368	21.906045232442725
50-51	21.600105421110577	27.386449136439573	28.865660348484784	22.147785093965066
52-53	21.75517747643665	27.450677847516403	28.68000173425589	22.114142941791062
54-55	21.76213356367759	27.96871818437261	28.457023636500438	21.81212461544936
56-57	21.478804549474752	27.60748816241494	28.57255788314526	22.341149404965048
58-59	21.425456919171012	27.113710677497473	29.026723704889246	22.43410869844227
60-61	21.926076405564032	27.66584359341598	28.43589166032716	21.972188340692828
62-63	21.524806991567385	27.51161705495014	28.67837991063346	22.28519604284902
64-65	21.56872152613545	27.283365106592562	28.87170255134318	22.276210815928803
66-67	21.46828847434138	27.361805546120298	28.5468692680806	22.623036711457726
68-69	21.9566148713226	27.53322285584726	28.48591072383134	22.0242515489988
70-71	22.090265417589436	27.206449300960582	28.521861116017444	22.181424165432535
72-73	21.65086072200106	27.463069455578047	28.831469681118467	22.054600141302426
74-75	21.69671607722721	27.58067995000068	28.499485401013576	22.223118571758533
76-77	21.808476535847227	27.54506882764366	28.278075483890962	22.36837915261815
78-79	21.925186138775288	27.732299832882706	28.259763793970187	22.082750234371815
80-81	21.782883200366843	27.451883680150136	28.449408338039174	22.31582478144385
82-83	21.566580277441883	27.552572315593537	28.61998308446896	22.260864322495618
84-85	21.727583524680373	27.57711342237198	28.358947228977865	22.336355823969782
86-87	21.909738100120883	27.650832430018006	28.27221996481847	22.167209505042642
88-89	21.391708039288034	27.63248119815508	28.288258299327325	22.687552463229565
90-91	21.4591265145542	27.43811541203389	28.389147391487292	22.713610681924617
92-93	21.727072010869566	27.744607676630434	28.118546195652172	22.409774116847824
94-95	21.445637031636842	27.441101529935715	28.350919264927732	22.76234217349971
96-97	21.44739585168793	27.393244716105443	28.416035339328804	22.743324092877828
98-99	21.379464952921836	27.48480829064823	28.36905239059252	22.76667436583742
100	21.62759337509001	27.319431801198352	28.129458159535876	22.923516664175757
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	66.0
1	65.0
2	240.5
3	432.0
4	258.0
5	115.5
6	300.5
7	715.5
8	1222.5
9	1355.5
10	992.5
11	679.0
12	631.5
13	687.5
14	799.5
15	944.5
16	1079.0
17	1212.0
18	1368.5
19	1534.0
20	1784.5
21	2166.5
22	2691.5
23	3574.5
24	4835.5
25	6266.5
26	8149.5
27	10604.5
28	13589.5
29	16773.5
30	19815.0
31	22908.0
32	25916.0
33	28498.0
34	30849.5
35	32908.5
36	34899.0
37	36564.0
38	37704.0
39	38220.0
40	38636.5
41	39211.0
42	39258.5
43	39248.5
44	38541.5
45	38038.5
46	38297.0
47	38517.0
48	38883.5
49	37790.0
50	36093.0
51	35028.5
52	32947.5
53	31104.5
54	30267.5
55	29280.0
56	27465.5
57	25023.0
58	23083.0
59	21739.0
60	19754.0
61	17065.0
62	14580.5
63	12551.5
64	10310.5
65	7954.0
66	6371.0
67	4962.5
68	3585.5
69	2563.5
70	1767.5
71	1248.0
72	932.0
73	669.5
74	490.0
75	361.5
76	242.5
77	148.5
78	99.0
79	59.0
80	30.5
81	15.0
82	9.0
83	4.0
84	2.0
85	1.0
86	0.5
87	0.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.245866224643687E-4
2	5.095039469572425E-4
3	1.698346489857475E-4
4	0.011633673455523703
5	4.245866224643687E-4
6	0.0033966929797149496
7	5.095039469572425E-4
8	0.14181193190309915
9	0.0011888425429002322
10-11	0.0
12-13	1.698346489857475E-4
14-15	1.2737598673931061E-4
16-17	0.0
18-19	2.9721063572505805E-4
20-21	0.0
22-23	2.1229331123218435E-4
24-25	5.095039469572425E-4
26-27	0.006029130038994035
28-29	0.007133055257401394
30-31	0.012780057336177498
32-33	0.017025923560821183
34-35	0.024880776076412002
36-37	0.036004945584978465
38-39	0.04109998505455089
40-41	0.053795125066235516
42-43	0.07200989116995693
44-45	0.11141152973465035
46-47	0.11905408893900897
48-49	0.11536018532356898
50-51	0.11727082512465864
52-53	0.11200595100610046
54-55	0.11935129957473403
56-57	0.09956556296789447
58-59	0.09298447031969674
60-61	0.0960414940014402
62-63	0.09451298216056847
64-65	8.491732449287375E-5
66-67	0.0
68-69	4.2458662246436874E-5
70-71	0.0
72-73	0.0
74-75	0.0
76-77	4.2458662246436874E-5
78-79	0.0
80-81	8.491732449287375E-5
82-83	0.0
84-85	0.0
86-87	8.067145826823005E-4
88-89	4.670452847108055E-4
90-91	4.2458662246436874E-5
92-93	0.00135867719188598
94-95	0.0014860531786252905
96-97	3.821279602179318E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1177616.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.665496359596
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.98245872491673	61.866095074702756
2	10.828782255511031	15.954152396444115
3	2.5262570929549337	5.582939480534258
4	0.9757558365989415	2.8751815211533556
5	0.5082309040639179	1.8719540906577368
6	0.2782080046535733	1.2296598452410967
7	0.19428058595424116	1.0018243058146632
8	0.13872066754080542	0.8175141463782343
9	0.09490429720136981	0.6292054943997774
>10	0.44835500594421346	5.648092418966269
>50	0.017122757085866202	0.8433709050840772
>100	0.006000693282486818	0.7534273111106448
>500	5.76983932455252E-4	0.26581881383002676
>1k	3.461903594731512E-4	0.6607641956830183
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3430	0.29126642301055694	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2826	0.2399763590168612	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1517	0.12881958125568946	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	4.245866224643687E-5	0.0
18-19	0.0	0.0	0.0	8.491732449287373E-5	0.0
20-21	0.0	0.0	0.0	1.273759867393106E-4	0.0
22-23	0.0	0.0	0.0	2.1229331123218435E-4	0.0
24-25	0.0	0.0	0.0	3.3966929797149493E-4	0.0
26-27	0.0	0.0	0.0	3.821279602179318E-4	0.0
28-29	0.0	0.0	0.0	0.001316218529639543	0.0
30-31	0.0	0.0	0.0	0.00433078354913656	0.0
32-33	0.0	0.0	0.0	0.010402372250377033	0.0
34-35	0.0	0.0	0.0	0.017790179481257048	0.0
36-37	0.0	0.0	0.0	0.03180153802258122	0.0
38-39	0.0	0.0	0.0	0.058975081860300815	0.0
40-41	0.0	0.0	0.0	0.10007506691485171	0.0
42-43	0.0	0.0	0.0	0.14261864648578143	0.0
44-45	0.0	0.0	0.0	0.1888561296721512	0.0
46-47	0.0	0.0	0.0	0.2423965027649081	0.0
48-49	0.0	0.0	0.0	0.30328222442629854	0.0
50-51	0.0	0.0	0.0	0.37614128884118425	0.0
52-53	0.0	0.0	0.0	0.4549021073083246	0.0
54-55	0.0	0.0	0.0	0.5357434002255403	0.0
56-57	0.0	0.0	0.0	0.6269021480686403	0.0
58-59	0.0	0.0	0.0	0.7332610969959648	0.0
60-61	0.0	0.0	0.0	0.8526548552329452	0.0
62-63	0.0	0.0	0.0	0.9766341489925409	0.0
64-65	0.0	0.0	0.0	1.1113130256382386	0.0
66-67	0.0	0.0	0.0	1.2609798100569285	0.0
68-69	0.0	0.0	0.0	1.4279697286721649	0.0
70-71	0.0	0.0	0.0	1.6077397046235786	0.0
72-73	0.0	0.0	0.0	1.810819486148286	0.0
74-75	0.0	0.0	0.0	2.0020108422439913	0.0
76-77	0.0	0.0	0.0	2.1959195527234687	0.0
78-79	0.0	0.0	0.0	2.41920965747748	0.0
80-81	0.0	0.0	0.0	2.6696733060692113	0.0
82-83	0.0	0.0	0.0	2.945484776022065	0.0
84-85	0.0	0.0	0.0	3.2062658795396803	0.0
86-87	0.0	0.0	0.0	3.4727789024605644	0.0
88	0.0	0.0	0.0	3.687704650752028	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAACG	175	0.0	42.990433	2
TAACGCA	155	0.0	39.436787	4
TACACCG	170	0.0	38.719707	5
GGTATCA	2760	0.0	36.08367	1
ATAACGC	185	0.0	35.583298	3
GTATCAA	4475	0.0	35.48196	1
TCAACGC	5295	0.0	29.748749	4
ATCAACG	5345	0.0	29.470465	3
TATCAAC	5450	0.0	29.075235	2
CAACGCA	5435	0.0	28.979988	5
AACGCAG	5590	0.0	28.344645	6
GTACATG	3660	0.0	25.670414	1
ACACCGT	220	8.731149E-11	25.645521	6
TACATGG	3540	0.0	24.971489	2
ACGCAGA	6310	0.0	24.961369	7
ACATGGG	3525	0.0	24.677574	3
CGCAGAG	6470	0.0	24.271418	8
GTCTCGC	375	0.0	23.801605	1
TACCTGG	810	0.0	23.800644	2
GTACCTG	860	0.0	22.942186	1
>>END_MODULE