##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032932_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 638511 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.674278125200665 34.0 31.0 34.0 31.0 34.0 2 32.637876246454645 34.0 31.0 34.0 31.0 34.0 3 32.74139678094817 34.0 31.0 34.0 31.0 34.0 4 36.13364061073341 37.0 35.0 37.0 35.0 37.0 5 36.18069383299583 37.0 35.0 37.0 35.0 37.0 6 36.32813217000177 37.0 37.0 37.0 35.0 37.0 7 36.31462887875072 37.0 37.0 37.0 35.0 37.0 8 36.26302914123641 37.0 37.0 37.0 35.0 37.0 9 38.05744145363197 39.0 39.0 39.0 35.0 39.0 10-11 38.03659686364057 39.0 39.0 39.0 35.0 39.0 12-13 38.07258528044153 39.0 39.0 39.0 35.0 39.0 14-15 39.28058404632027 41.0 39.0 41.0 36.0 41.0 16-17 39.47609124979836 41.0 39.0 41.0 36.5 41.0 18-19 39.62366035980586 41.0 40.0 41.0 37.0 41.0 20-21 39.610753769316425 41.0 40.0 41.0 37.0 41.0 22-23 39.50429201689556 41.0 39.0 41.0 36.5 41.0 24-25 39.35834778100926 41.0 39.0 41.0 36.0 41.0 26-27 39.14758633758855 41.0 39.0 41.0 35.5 41.0 28-29 39.160412271675824 41.0 39.0 41.0 35.5 41.0 30-31 39.029210146732005 41.0 39.0 41.0 35.0 41.0 32-33 38.68883073275167 41.0 38.0 41.0 35.0 41.0 34-35 38.30653896330682 40.0 38.0 41.0 35.0 41.0 36-37 38.06353766810595 40.0 37.5 41.0 34.0 41.0 38-39 37.9110109301171 40.0 38.0 41.0 34.0 41.0 40-41 38.00554806416804 40.0 38.0 41.0 34.0 41.0 42-43 37.74029421576135 40.0 38.0 41.0 33.0 41.0 44-45 37.35371982628334 40.0 38.0 41.0 33.0 41.0 46-47 36.76701654317623 40.0 37.5 41.0 31.5 41.0 48-49 36.13805478684001 40.0 37.0 41.0 28.0 41.0 50-51 35.84184610758468 40.0 37.0 41.0 27.5 41.0 52-53 35.93860168423097 40.0 37.0 41.0 27.5 41.0 54-55 35.781894125551474 40.0 37.0 41.0 26.5 41.0 56-57 35.39143178426057 40.0 36.5 41.0 24.0 41.0 58-59 35.21352333789081 39.5 36.0 41.0 23.5 41.0 60-61 34.67214973586986 39.0 35.0 41.0 22.0 41.0 62-63 34.69142035141133 39.0 35.0 41.0 22.0 41.0 64-65 34.32005008527653 38.5 35.0 40.0 22.0 41.0 66-67 33.69748445993883 37.0 35.0 39.0 20.0 41.0 68-69 32.99692096142431 37.0 34.0 39.0 15.5 40.0 70-71 32.12916848730875 35.5 33.5 38.0 8.0 39.0 72-73 31.63453801109143 35.0 33.0 37.0 2.0 39.0 74-75 31.42330124304828 35.0 33.0 37.0 2.0 37.0 76-77 31.058678707179673 35.0 33.0 36.0 2.0 37.0 78-79 30.701662931413868 35.0 32.0 36.0 2.0 36.5 80-81 30.27108068615889 35.0 31.5 35.0 2.0 36.0 82-83 29.963446988383915 35.0 31.0 35.0 2.0 36.0 84-85 29.874886258811514 35.0 31.0 35.0 2.0 35.0 86-87 30.112202452267855 35.0 32.0 35.0 2.0 35.5 88-89 29.951081500553634 35.0 32.0 35.0 2.0 35.0 90-91 29.714004144016315 35.0 32.0 35.0 2.0 35.0 92-93 29.55528330757027 35.0 31.0 35.0 2.0 35.0 94-95 29.354763661080234 35.0 31.0 35.0 2.0 35.0 96-97 29.401903021247875 35.0 31.5 35.0 2.0 35.0 98-99 29.438315863000014 35.0 32.0 35.0 2.0 35.0 100 29.2877115664413 35.0 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.05530805992130183 1101 2 0.15041260150378122 1101 3 0.12341875714652417 1101 4 0.14624410073422212 1101 5 0.05496095365804621 1101 6 0.1041169596471434 1101 7 0.0015738266342353313 1101 8 -0.23960449198540346 1101 9 -0.020694437445918368 1101 10-11 0.10443992752889386 1101 12-13 0.14045353415933448 1101 14-15 0.27015160020468443 1101 16-17 0.1809344584885153 1101 18-19 0.09636792326145383 1101 20-21 0.23061108795649687 1101 22-23 0.23425717649553945 1101 24-25 0.18867254906907505 1101 26-27 0.23921663712785346 1101 28-29 0.196456450975397 1101 30-31 0.28738317874260844 1101 32-33 0.3534148933223378 1101 34-35 0.4346492049835504 1101 36-37 0.4429189350692937 1101 38-39 0.3248376167263558 1101 40-41 0.3119348960932058 1101 42-43 0.35129501983024625 1101 44-45 0.340321047506535 1101 46-47 0.7518943441539534 1101 48-49 0.7567359609463153 1101 50-51 0.7802110994858467 1101 52-53 0.7835971874768362 1101 54-55 0.8149181987881349 1101 56-57 0.8823453721744414 1101 58-59 0.8330577338723231 1101 60-61 0.9253010840427862 1101 62-63 0.7856316135300219 1101 64-65 0.9408930458617917 1101 66-67 0.7952147986763549 1101 68-69 0.6535178044338608 1101 70-71 0.8201843098086741 1101 72-73 0.8983817041565842 1101 74-75 0.84445486938899 1101 76-77 0.7843372432148996 1101 78-79 0.7705498284713315 1101 80-81 0.8750862232102321 1101 82-83 0.9730201258814937 1101 84-85 0.6805871920625073 1101 86-87 0.6877632562174796 1101 88-89 0.7503815076257716 1101 90-91 0.8455525322345778 1101 92-93 0.8289540916423661 1101 94-95 0.7844823929346845 1101 96-97 0.8827216836412006 1101 98-99 0.888637399298009 1101 100 0.9211714567754612 1102 1 0.22280812810869577 1102 2 0.22036529460291376 1102 3 0.30310164029150855 1102 4 0.2039836733659186 1102 5 0.1321566636100684 1102 6 0.12004402034605022 1102 7 0.16959211903997584 1102 8 -0.004515560466764157 1102 9 0.1350940664619813 1102 10-11 0.22239427530886502 1102 12-13 0.26631307418230676 1102 14-15 0.28842279209007415 1102 16-17 0.23954001106402956 1102 18-19 0.20325469884612346 1102 20-21 0.2838124104889417 1102 22-23 0.2730118624849993 1102 24-25 0.33162212373124333 1102 26-27 0.30045422323338755 1102 28-29 0.2248913532249759 1102 30-31 0.23545014179715906 1102 32-33 0.07828101873260351 1102 34-35 0.1654226319002916 1102 36-37 0.37686353053619825 1102 38-39 0.21925852389149014 1102 40-41 0.2639256650349111 1102 42-43 0.03794059386206072 1102 44-45 -0.008064274928941018 1102 46-47 -0.003409877706616271 1102 48-49 -0.002385239879203027 1102 50-51 0.13258235410364705 1102 52-53 0.31884401070175983 1102 54-55 0.36314487187956956 1102 56-57 0.19908189304129564 1102 58-59 0.12754016122244138 1102 60-61 0.127732040514978 1102 62-63 0.11324402424241953 1102 64-65 0.20960215236444668 1102 66-67 0.3089772883345958 1102 68-69 0.17788040379805636 1102 70-71 0.12359019453257503 1102 72-73 0.15847874239911164 1102 74-75 0.2088331670072776 1102 76-77 0.18065072946983562 1102 78-79 0.18029926251593054 1102 80-81 0.21837794707855096 1102 82-83 0.24146936263722907 1102 84-85 0.1584270456262793 1102 86-87 0.1924742477228456 1102 88-89 0.25239682403045194 1102 90-91 0.190084509093289 1102 92-93 0.20762598297892865 1102 94-95 0.32792837184810963 1102 96-97 0.39384153284098744 1102 98-99 0.2829726933978307 1102 100 0.2977840887180747 1103 1 0.24260253724937542 1103 2 0.2268714791944575 1103 3 0.2711195694609998 1103 4 0.1679295199560329 1103 5 0.2288975682441574 1103 6 0.17830953327980836 1103 7 0.2876524984911839 1103 8 0.0902271793916185 1103 9 0.21580105660837035 1103 10-11 0.2015221721475342 1103 12-13 0.21419284698038865 1103 14-15 0.2900950759152323 1103 16-17 0.3412999111272015 1103 18-19 0.33601402432972804 1103 20-21 0.2792122006673523 1103 22-23 0.38280229816496103 1103 24-25 0.3211224681931313 1103 26-27 0.3009223197741875 1103 28-29 0.4176699457051356 1103 30-31 0.437927980463229 1103 32-33 0.49500557214705765 1103 34-35 0.516200601204595 1103 36-37 0.5066820663016287 1103 38-39 0.3629248561013938 1103 40-41 0.4219750674435687 1103 42-43 0.370740797394717 1103 44-45 0.43926521643950167 1103 46-47 0.2910214171556049 1103 48-49 0.16469735379013173 1103 50-51 0.07617707826257458 1103 52-53 0.20713830978615277 1103 54-55 0.299751736533203 1103 56-57 0.4154289676880438 1103 58-59 0.4026865932548205 1103 60-61 0.3331513835504154 1103 62-63 0.376501518789631 1103 64-65 0.402810250220206 1103 66-67 0.48567765795220197 1103 68-69 0.4184398613491993 1103 70-71 0.3048178795486223 1103 72-73 0.021122065698740755 1103 74-75 0.07394703953065118 1103 76-77 0.08893739607933071 1103 78-79 0.10063923638828598 1103 80-81 0.19606153382719782 1103 82-83 0.2444744709640574 1103 84-85 0.27964689806509924 1103 86-87 0.21978035570378296 1103 88-89 0.27934131850047805 1103 90-91 0.2601217567142129 1103 92-93 0.12237381115390988 1103 94-95 0.2700180405643984 1103 96-97 0.3842058906125345 1103 98-99 0.1009767583338963 1103 100 0.337451518632232 1104 1 0.04889859363262872 1104 2 0.1285135916841469 1104 3 0.20532434077773587 1104 4 0.12524899000658252 1104 5 0.03174718152278899 1104 6 0.0036814446300894588 1104 7 0.149731105076242 1104 8 -0.04068506226355595 1104 9 0.014146763918148508 1104 10-11 0.14765246707448654 1104 12-13 0.1868399921074868 1104 14-15 0.3273540325149611 1104 16-17 0.2585465214327556 1104 18-19 0.2893195646600333 1104 20-21 0.2238421163471429 1104 22-23 0.30347735809708354 1104 24-25 0.19869761172571287 1104 26-27 0.2521793718334706 1104 28-29 0.20026720737459414 1104 30-31 0.20629932155866726 1104 32-33 0.16196290269002134 1104 34-35 0.2586045852374568 1104 36-37 0.31667093802750657 1104 38-39 0.3597815338963315 1104 40-41 0.24248873971866658 1104 42-43 0.38977236072345534 1104 44-45 0.4698970366597379 1104 46-47 0.5267207544419676 1104 48-49 0.49696768780444245 1104 50-51 0.44828497269345746 1104 52-53 0.5718896399194122 1104 54-55 0.49108607750131483 1104 56-57 0.670874070970612 1104 58-59 0.6242056857191542 1104 60-61 0.6278361101325984 1104 62-63 0.5207275500927437 1104 64-65 0.5385655056999141 1104 66-67 0.5343680095724466 1104 68-69 0.5792337336010434 1104 70-71 0.4099579241724456 1104 72-73 0.48178693855405896 1104 74-75 0.39208418858862615 1104 76-77 0.421503930424727 1104 78-79 0.43698024878440833 1104 80-81 0.5753820447312492 1104 82-83 0.5998844177587266 1104 84-85 0.6049883589140599 1104 86-87 0.5810312622982536 1104 88-89 0.5579537168373285 1104 90-91 0.6832923736413719 1104 92-93 0.5872740719209304 1104 94-95 0.6546701574278835 1104 96-97 0.49567825539846666 1104 98-99 0.6104316389956139 1104 100 0.5141087179612107 1105 1 0.17834291979826844 1105 2 0.2390849475573873 1105 3 0.21754542636946894 1105 4 0.14448804763028278 1105 5 0.19722904072715863 1105 6 0.16952160207360834 1105 7 0.2327810020005927 1105 8 0.17936977294521483 1105 9 0.2684736046104419 1105 10-11 0.25691785182967664 1105 12-13 0.23967470237921873 1105 14-15 0.2471172960407273 1105 16-17 0.2917436321988376 1105 18-19 0.3314248004877811 1105 20-21 0.31745791678022073 1105 22-23 0.31383944702774613 1105 24-25 0.3545417675698701 1105 26-27 0.28940863597196653 1105 28-29 0.3293843916697128 1105 30-31 0.3628990193418886 1105 32-33 0.4463037118209101 1105 34-35 0.38936137343127797 1105 36-37 0.4347457860103958 1105 38-39 0.49896611980222616 1105 40-41 0.40371645642419196 1105 42-43 0.4137238191011505 1105 44-45 0.5186632444623385 1105 46-47 0.5050021691488098 1105 48-49 0.6650433146965469 1105 50-51 0.5428744096459539 1105 52-53 0.5641044423101462 1105 54-55 0.5595041648118411 1105 56-57 0.5961948517492033 1105 58-59 0.5121557005184343 1105 60-61 0.5411616889919628 1105 62-63 0.63834543800251 1105 64-65 0.5192680298850476 1105 66-67 0.6975079484021123 1105 68-69 0.6382023539451112 1105 70-71 0.7272426985743792 1105 72-73 0.727169305087962 1105 74-75 0.8309579897175254 1105 76-77 0.8678539618407335 1105 78-79 0.8934846600069335 1105 80-81 0.8945959176236506 1105 82-83 0.9558277547096594 1105 84-85 0.8504797727381401 1105 86-87 0.7891350337766774 1105 88-89 0.7527835771010167 1105 90-91 0.8030418764376535 1105 92-93 0.7284422799623869 1105 94-95 0.6690760833691947 1105 96-97 0.6529218514665303 1105 98-99 0.650889481832504 1105 100 0.7055853740520242 1106 1 0.14281466427001277 1106 2 0.1575723928740942 1106 3 0.21288431292786925 1106 4 0.1266478127168753 1106 5 0.1810667587112178 1106 6 0.09743674559850746 1106 7 0.09819240009491637 1106 8 0.0906156529715858 1106 9 0.11650260922481692 1106 10-11 0.2595798042477213 1106 12-13 0.21549180551339475 1106 14-15 0.1887724767202954 1106 16-17 0.20413231800215925 1106 18-19 0.2357047789140978 1106 20-21 0.1457529967826119 1106 22-23 0.20763521618936664 1106 24-25 0.2386203916484959 1106 26-27 0.2940991135892759 1106 28-29 0.3430357370283659 1106 30-31 0.31994702078013404 1106 32-33 0.3214843913918415 1106 34-35 0.3972177886047632 1106 36-37 0.43948060952570955 1106 38-39 0.5482535890896969 1106 40-41 0.4901879611883828 1106 42-43 0.4546574800348111 1106 44-45 0.46346878170690076 1106 46-47 0.60388513193422 1106 48-49 0.8450960503590395 1106 50-51 0.7136948087590369 1106 52-53 0.6867076663767833 1106 54-55 0.6630710132567401 1106 56-57 0.7216937431017598 1106 58-59 0.568498362470848 1106 60-61 0.6656783786306022 1106 62-63 0.8832384685053043 1106 64-65 0.8731297186247886 1106 66-67 0.8510753961158954 1106 68-69 0.9639289674766047 1106 70-71 1.0473014269257916 1106 72-73 0.992705351111816 1106 74-75 1.0132633771448596 1106 76-77 0.9254149374504905 1106 78-79 0.7656504177824459 1106 80-81 0.9304855322937513 1106 82-83 0.8454322372409742 1106 84-85 0.8202532490969823 1106 86-87 0.9650713914203486 1106 88-89 0.9226337598780283 1106 90-91 0.8102067353098334 1106 92-93 0.7671658372225316 1106 94-95 0.8635909752011592 1106 96-97 0.9407051558352038 1106 98-99 0.8964339176208433 1106 100 0.9323643037577831 1107 1 -0.040639355787781994 1107 2 -0.04301120959629401 1107 3 -0.007238073609613593 1107 4 -0.021864850890125354 1107 5 0.01249630146151759 1107 6 -0.02062886718408663 1107 7 -0.0882340263315129 1107 8 -0.06468606383956654 1107 9 0.013628792011374458 1107 10-11 -0.020242848593802876 1107 12-13 0.06078502325944868 1107 14-15 0.010544248492067254 1107 16-17 0.08651602246133194 1107 18-19 0.049493662136939065 1107 20-21 -0.05974136550420184 1107 22-23 0.0685599144725586 1107 24-25 0.009868621009928802 1107 26-27 0.036815981400479814 1107 28-29 0.12109115093284117 1107 30-31 0.04857073883781737 1107 32-33 0.1290213623250338 1107 34-35 0.2729359596059524 1107 36-37 0.25653709997842356 1107 38-39 0.29165718136120944 1107 40-41 0.26180958281000244 1107 42-43 0.25167884101843185 1107 44-45 0.040842003985780195 1107 46-47 0.06788479197160768 1107 48-49 0.11621169430487299 1107 50-51 0.22767803727055735 1107 52-53 0.11014468830436641 1107 54-55 0.10676810657647451 1107 56-57 0.15523672004096056 1107 58-59 0.16305726099200513 1107 60-61 0.027774248084966757 1107 62-63 0.10242595788147213 1107 64-65 0.10131375171447843 1107 66-67 0.017492190268548313 1107 68-69 0.004962453793112331 1107 70-71 -0.12268780759175257 1107 72-73 0.03239598514206676 1107 74-75 -0.051783367713202466 1107 76-77 -0.09118883613441398 1107 78-79 -0.029417858040545752 1107 80-81 0.060774500507250195 1107 82-83 0.05214876508957289 1107 84-85 0.09291912251757495 1107 86-87 0.06974021118351104 1107 88-89 0.08685949504716106 1107 90-91 -0.014790148366294886 1107 92-93 0.15967476124088975 1107 94-95 0.2809239685718907 1107 96-97 0.3977435149490347 1107 98-99 0.46550015366821285 1107 100 0.4272637314873968 1108 1 0.02608866998303938 1108 2 0.05643528015161792 1108 3 -0.030976377030384583 1108 4 -0.02236339361139983 1108 5 -0.00897938499346651 1108 6 0.021517167483807498 1108 7 -0.01675706156186152 1108 8 0.008638065628140623 1108 9 0.0631890517649154 1108 10-11 -0.04906884440092796 1108 12-13 0.015820304744330826 1108 14-15 -0.055173908689504 1108 16-17 -0.05715695907979779 1108 18-19 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-0.24034463062493572 2215 1 -0.15083831563296712 2215 2 -0.29687147406976777 2215 3 -0.2068480155544563 2215 4 -0.11818500086593531 2215 5 -0.13020749631304085 2215 6 -0.13311072494896337 2215 7 -0.06286009470758103 2215 8 -0.11061615576022632 2215 9 -0.2122369957647905 2215 10-11 -0.21172910081118346 2215 12-13 -0.2758365901900035 2215 14-15 -0.3707324609096432 2215 16-17 -0.2782814900366475 2215 18-19 -0.13820287874355586 2215 20-21 -0.16960519795058104 2215 22-23 -0.21374082331166733 2215 24-25 -0.23370852380541862 2215 26-27 -0.29701878315361796 2215 28-29 -0.25281275569512474 2215 30-31 -0.3681589217008039 2215 32-33 -0.19311670008425352 2215 34-35 -0.20292730841533313 2215 36-37 -0.2389449795248808 2215 38-39 -0.15892286621176055 2215 40-41 -0.23939234026691736 2215 42-43 -0.2971590480317161 2215 44-45 -0.29840015453704183 2215 46-47 -0.10256150701242461 2215 48-49 -0.086197695309707 2215 50-51 -0.07229878098456055 2215 52-53 -0.05317322412910386 2215 54-55 -0.007679159993436713 2215 56-57 -0.171575487667468 2215 58-59 -0.20455733745985327 2215 60-61 -0.09627328457105477 2215 62-63 0.028639975781807436 2215 64-65 -0.1473835350134678 2215 66-67 -0.1691914091509048 2215 68-69 -0.2749873526897204 2215 70-71 -0.27156248506312863 2215 72-73 -0.17830339802193862 2215 74-75 -0.05105011904363366 2215 76-77 -0.05101134460079493 2215 78-79 -0.21651217750515173 2215 80-81 -0.21652200408878386 2215 82-83 -0.24339832408958628 2215 84-85 -0.3278925409238056 2215 86-87 -0.4883199807210197 2215 88-89 -0.4631601590408927 2215 90-91 -0.5760302709271734 2215 92-93 -0.6273278391461474 2215 94-95 -0.6467338688736852 2215 96-97 -0.5113916043865601 2215 98-99 -0.5307403720534474 2215 100 -0.5367642478707708 2216 1 -0.14306771376706706 2216 2 -0.18611241268916245 2216 3 -0.21818802223286582 2216 4 -0.1650520955576127 2216 5 -0.2447870350983763 2216 6 -0.1503936262097625 2216 7 -0.1612849778354999 2216 8 -0.09548729291037006 2216 9 -0.20049476699908553 2216 10-11 -0.27874524534804834 2216 12-13 -0.25357366090013755 2216 14-15 -0.3549955217438381 2216 16-17 -0.2526875800332036 2216 18-19 -0.31357613578118304 2216 20-21 -0.357669004539936 2216 22-23 -0.3722798843887105 2216 24-25 -0.35931343799804694 2216 26-27 -0.3883779017054181 2216 28-29 -0.34502501512077544 2216 30-31 -0.47015985010021666 2216 32-33 -0.6167903158331498 2216 34-35 -0.7133797070756103 2216 36-37 -0.8616546535985066 2216 38-39 -0.7305667782389591 2216 40-41 -0.7241809459982065 2216 42-43 -0.8185356341527807 2216 44-45 -0.6492627185384166 2216 46-47 -0.6984788263413435 2216 48-49 -0.7823742455090468 2216 50-51 -0.8673470223380448 2216 52-53 -0.8629085797035501 2216 54-55 -0.7705717692258247 2216 56-57 -1.045630645217102 2216 58-59 -1.0399292917026628 2216 60-61 -1.1218921753487905 2216 62-63 -1.0437173112681606 2216 64-65 -1.0887909708097183 2216 66-67 -1.2516115212671437 2216 68-69 -1.1371674722938678 2216 70-71 -1.1406812518856242 2216 72-73 -1.1860420624422332 2216 74-75 -1.1703680996357875 2216 76-77 -1.1569212898581114 2216 78-79 -1.1907449831433397 2216 80-81 -1.3768350163527643 2216 82-83 -1.4756360449322834 2216 84-85 -1.457890909268169 2216 86-87 -1.5044714742551122 2216 88-89 -1.5113917099485512 2216 90-91 -1.702859243060015 2216 92-93 -1.7751251672784178 2216 94-95 -1.68578166500021 2216 96-97 -1.706302672830084 2216 98-99 -1.8448745573892893 2216 100 -1.8592464387921872 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 2.0 5 26.0 6 126.0 7 310.0 8 408.0 9 471.0 10 806.0 11 1380.0 12 2277.0 13 3483.0 14 3678.0 15 4830.0 16 6301.0 17 9624.0 18 12282.0 19 5788.0 20 2634.0 21 2168.0 22 2219.0 23 2445.0 24 2821.0 25 3849.0 26 4430.0 27 5592.0 28 4844.0 29 5934.0 30 7151.0 31 8996.0 32 11251.0 33 14572.0 34 19779.0 35 30993.0 36 57654.0 37 174934.0 38 196005.0 39 28359.0 40 87.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.348650219025195 17.439167062117956 13.87759960282595 15.33458311603089 2 14.148496646860014 28.050480340043787 29.98358668516819 27.817436327928007 3 33.62160909243252 22.576815675268477 30.95539765296965 12.846177579329343 4 11.265406235952504 15.884496890168359 44.061560726687226 28.788536147191913 5 8.697477083258288 42.93218398545356 34.68184373859645 13.688495192691702 6 24.66385060792725 38.53207848001742 13.162333726453882 23.64173718560145 7 20.838521233193163 36.69462259496793 22.584474671302495 19.882381500536408 8 22.62215070559931 20.1032285991211 20.45924336667639 36.8153773286032 9 17.034322362393404 25.678890533355258 28.874166999475335 28.412620104776 10-11 26.22687784548739 21.59696543990628 25.170122362809728 27.006034351796604 12-13 35.66660219369924 21.899718172214865 23.481602886718385 18.952076747367506 14-15 24.73712236300136 25.124508621634746 34.62639582778657 15.511973187577329 16-17 16.957264636004705 28.010089097916875 32.86858018107754 22.164066085000886 18-19 16.18832609381693 34.2966706055274 30.053350811538433 19.461652489117235 20-21 22.31159682448697 22.81832262874093 26.885049748555623 27.985030798216474 22-23 17.769310160670685 24.51296845316682 31.96593324155731 25.751788144605182 24-25 28.005008543340814 24.032942370428607 27.592015147789617 20.37003393844096 26-27 23.521649684755904 17.95830210650873 40.19837941590371 18.32166879283165 28-29 16.976668240755753 33.791463738391094 30.918085500446775 18.31378252040638 30-31 20.623404288645585 22.902040944191217 29.90124210954999 26.573312657613208 32-33 21.63036165601913 24.421512490787666 33.61403047099006 20.334095382203145 34-35 26.33634812059462 21.148470244151554 31.355119068039887 21.160062567213934 36-37 21.825267511632642 19.629948769368156 39.21297529336195 19.331808425637252 38-39 20.230953503784466 25.158671841094716 35.436110593215695 19.174264061905127 40-41 19.42297130760631 27.767292567147784 31.93557510531565 20.874161019930256 42-43 18.16574765729008 27.450863623292086 25.89435923876718 28.48902948065065 44-45 22.477169218227 19.922316550259076 29.412318039649133 28.188196191864794 46-47 28.000012543087465 26.281295983154635 29.81970095711597 15.898990516641934 48-49 24.183273990665093 23.775087367772464 30.54250330421858 21.499135337343862 50-51 13.441008571477846 26.591649903655146 38.832933790938725 21.134407733928285 52-53 21.116419805829807 30.87854760383637 24.728988739383155 23.276043850950664 54-55 16.858627415318814 23.15821161359916 29.63214404425184 30.351016926830187 56-57 24.43511161380893 20.076690158122503 27.609162373224915 27.879035854843654 58-59 30.87529792765403 20.83436202138574 25.823636233959324 22.4667038170009 60-61 21.91586917162407 24.8229051549139 34.525461057944774 18.735764615517255 62-63 16.915445501563994 26.093131196121323 32.706899213809244 24.28452408850544 64-65 15.988683045397808 34.317576361252975 26.8874772713391 22.806263322010114 66-67 21.05539293763146 24.558699850120043 23.345486608688027 31.04042060356047 68-69 17.75396195210419 25.565417040583483 27.799990916366358 28.88063009094597 70-71 26.604005255978365 25.039897511554226 24.207805347127927 24.148291885339486 72-73 22.683007810358788 19.81641663181997 35.2968860364191 22.203689521402136 74-75 17.210588384538404 33.39997274909908 26.535956310854473 22.853482555508048 76-77 20.150396782514317 24.118143618512445 26.05483695660686 29.67662264236638 78-79 21.600567570488213 25.54208149898749 28.671628210007345 24.185722720516953 80-81 25.560993563139185 23.11193246777654 26.676951026608826 24.65012294247545 82-83 21.181578988582793 21.744373619833677 33.471989475497644 23.602057916085887 84-85 19.816886474939352 26.581452786248004 30.082410483139682 23.519250255672965 86-87 19.553459350971835 28.630315806289186 27.169489521282802 24.646735321456177 88-89 17.949721890938022 28.448094269692575 22.35115115930328 31.25103268006612 90-91 21.79502840998077 22.146472714515713 25.114094106886682 30.94440476861684 92-93 25.565835685843048 27.05400527014381 25.998097110034806 21.38206193397833 94-95 22.507907148657527 24.649708811762487 27.352900410255526 25.48948362932446 96-97 14.492669641304978 26.758197613502706 33.83828575634799 24.910846988844323 98-99 20.85296886036419 29.69032640001503 23.141731309249174 26.314973430371598 100 19.687836231482308 19.350645486138845 24.67334157124936 36.28817671112949 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 407.0 1 280.0 2 413.5 3 712.5 4 433.0 5 185.0 6 425.0 7 796.0 8 1208.0 9 1315.0 10 805.0 11 321.0 12 241.0 13 258.0 14 273.5 15 285.0 16 309.5 17 354.5 18 413.0 19 440.0 20 512.0 21 660.5 22 867.0 23 1200.0 24 1568.0 25 1946.0 26 2439.0 27 2985.5 28 3670.0 29 4506.0 30 5282.5 31 6139.5 32 7081.0 33 7969.5 34 8709.5 35 8897.5 36 8668.5 37 8393.5 38 8100.5 39 7703.0 40 7322.0 41 7043.0 42 6615.0 43 6245.5 44 6105.5 45 6840.5 46 13762.5 47 58503.5 48 138398.5 49 134852.5 50 65511.5 51 30038.0 52 12710.0 53 5181.0 54 3811.5 55 3662.5 56 3479.0 57 3266.5 58 2937.0 59 2541.5 60 2423.0 61 1955.0 62 1504.0 63 1497.0 64 1234.5 65 857.5 66 735.5 67 688.5 68 626.0 69 459.0 70 271.5 71 140.5 72 84.0 73 66.0 74 53.5 75 37.0 76 30.0 77 19.0 78 11.5 79 9.0 80 2.5 81 3.0 82 2.5 83 0.0 84 0.5 85 1.0 86 0.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 7.830718656373969E-4 3 3.132287462549588E-4 4 0.008143947402628929 5 6.264574925099176E-4 6 0.004698431193824382 7 9.396862387648765E-4 8 0.14048309269534903 9 9.396862387648765E-4 10-11 0.0 12-13 7.83071865637397E-5 14-15 1.566143731274794E-4 16-17 0.0 18-19 2.3492155969121912E-4 20-21 0.0 22-23 0.0 24-25 3.132287462549588E-4 26-27 0.005873038992280477 28-29 0.007282568350427792 30-31 0.012685764223325832 32-33 0.01573974449931168 34-35 0.024666763767578006 36-37 0.034611776461172944 38-39 0.038213907043104976 40-41 0.04862876285608235 42-43 0.06491665766134022 44-45 0.10430517250290129 46-47 0.11111789773394663 48-49 0.10806391745796079 50-51 0.10931683244298063 52-53 0.10469670843571999 54-55 0.11072636180112794 56-57 0.09702260415247349 58-59 0.08809558488420716 60-61 0.09020987892142814 62-63 0.08793897051107968 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.566143731274794E-4 82-83 1.566143731274794E-4 84-85 0.0 86-87 3.9153593281869846E-4 88-89 3.9153593281869846E-4 90-91 4.6984311938243824E-4 92-93 0.001331222171583575 94-95 0.001017993425328616 96-97 3.132287462549588E-4 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 638511.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.18182718149122 #Duplication Level Percentage of deduplicated Percentage of total 1 85.02341259686254 20.560214697979536 2 8.907533272018552 4.308008603946713 3 2.041192571997529 1.480792980605635 4 0.9231444564923466 0.8929327884179825 5 0.5542976822607436 0.6701965379765217 6 0.4080551811823387 0.5920511923118041 7 0.2910607009008195 0.49268656979551256 8 0.20724429377285514 0.4009236557092304 9 0.17803481755646733 0.387468647139493 >10 1.1974188644159645 5.5854470275009565 >50 0.12556500995836017 2.168046044798323 >100 0.10355877109967851 4.803758966716008 >500 0.01165036174871383 1.9043182375874386 >1k 0.014886573345578785 8.087287552755859 >5k 0.005177938554983925 9.352711482310758 >10k+ 0.007766907832475888 38.31315501444823 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 58999 9.240091400148156 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 34253 5.364512122735552 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 25614 4.011520553287258 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 18287 2.864007041382216 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 15164 2.3749003541050975 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 14949 2.3412282638826896 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 14152 2.2164066085000886 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 14064 2.20262454366487 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 13981 2.1896255506952897 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 13155 2.0602620784919914 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 11813 1.8500855897549142 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 10358 1.6222116768544317 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 9387 1.470139120547649 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 8964 1.4038912407147255 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 8632 1.3518952688364023 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 8045 1.2599626318105719 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 7013 1.0983365987430131 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 6564 1.0280167452087747 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 5943 0.93075921949661 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 5208 0.8156476552479128 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 4768 0.7467373310718218 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 4688 0.7342081812216235 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4529 0.7093064958943542 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4293 0.672345503836269 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 2907 0.45527798268158265 No Hit CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT 2763 0.4327255129512255 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 2611 0.4089201282358487 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 2503 0.39200577593808095 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 2412 0.3777538679834803 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 2380 0.37274220804340097 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2269 0.3553580126262508 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1886 0.29537470771842617 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 1701 0.26640104868984243 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1637 0.2563777288096838 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 1289 0.20187592696132095 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1245 0.19498489454371185 No Hit ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT 1218 0.19075630646926992 RNA PCR Primer, Index 33 (95% over 22bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1197 0.18746740463359285 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 1179 0.1846483459172982 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 1073 0.1680472223657854 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 1055 0.16522816364949075 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1040 0.16287894805257858 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 1028 0.16099957557504882 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 965 0.15113287006801762 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 906 0.14189262205349634 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 821 0.12858040033766058 No Hit ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT 802 0.12560472724823848 RNA PCR Primer, Index 33 (95% over 24bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 771 0.12074968168128662 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 716 0.11213589115927525 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 708 0.11088297617425542 No Hit TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA 687 0.10759407433857836 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 680 0.106497773726686 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 660 0.10336548626413641 No Hit CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA 656 0.10273902877162648 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.566143731274794E-4 0.0 12-13 0.0 0.0 0.0 3.132287462549588E-4 0.0 14-15 0.0 0.0 0.0 3.132287462549588E-4 0.0 16-17 0.0 0.0 0.0 0.001017993425328616 0.0 18-19 0.0 0.0 0.0 0.0019576796640934924 0.0 20-21 0.0 0.0 0.0 0.00274075152973089 0.0 22-23 0.0 0.0 0.0 0.007282568350427792 0.0 24-25 0.0 0.0 0.0 0.015348208566492983 0.0 26-27 0.0 0.0 0.0 0.03923190046843359 0.0 28-29 0.0 0.0 0.0 0.11268404146522143 0.0 30-31 0.0 0.0 0.0 0.26640104868984243 0.0 32-33 0.0 0.0 0.0 0.5332719404990673 0.0 34-35 0.0 0.0 0.0 0.9154893181166808 0.0 36-37 0.0 0.0 0.0 1.3741345098205042 0.0 38-39 0.0 0.0 0.0 1.9456986645492402 0.0 40-41 0.0 0.0 0.0 2.5494470729556733 0.0 42-43 0.0 0.0 0.0 3.0728523079477097 0.0 44-45 0.0 0.0 0.0 3.6164607970731906 0.0 46-47 0.0 0.0 0.0 4.301492065132786 0.0 48-49 0.0 0.0 0.0 4.8346856984452895 0.0 50-51 0.0 0.0 0.0 5.376493122279804 0.0 52-53 0.0 0.0 0.0 5.862232600534682 0.0 54-55 0.0 0.0 0.0 6.435989356487202 0.0 56-57 0.0 0.0 0.0 7.293139820613897 0.0 58-59 0.0 0.0 0.0 7.859770622589117 0.0 60-61 0.0 0.0 0.0 8.475891566472622 0.0 62-63 0.0 0.0 0.0 9.050118165544525 0.0 64-65 0.0 0.0 0.0 9.608761634490243 0.0 66-67 0.0 0.0 0.0 10.35808310271867 0.0 68-69 0.0 0.0 0.0 11.300040249893893 0.0 70-71 0.0 0.0 0.0 12.132993793372393 0.0 72-73 0.0 0.0 0.0 12.797821807298543 0.0 74-75 0.0 0.0 0.0 13.66342944757412 0.0 76-77 0.0 0.0 0.0 14.349400401872481 0.0 78-79 0.0 0.0 0.0 15.652901829412492 0.0 80-81 0.0 0.0 0.0 16.847791189188598 0.0 82-83 0.0 0.0 0.0 17.677220909271725 0.0 84-85 0.0 0.0 0.0 18.633195042841862 0.0 86-87 0.0 0.0 0.0 19.501856663393426 0.0 88 0.0 0.0 0.0 20.323847200753 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACGTGG 55 1.2805685E-8 59.84557 8 TAGGACG 55 1.2805685E-8 59.84557 5 CAACCGT 55 8.777042E-7 51.296204 6 GGACGTG 65 4.7817593E-8 50.638557 7 AACCGTT 60 1.5989572E-6 47.021515 7 AGGACGT 75 1.4742909E-7 43.88675 6 ACAACCG 65 2.7742171E-6 43.404476 5 ACCGTTC 65 2.7742171E-6 43.404476 8 ACGTGGC 80 2.4671135E-7 41.101944 9 GTATAGA 50 0.0017054981 37.581863 1 CCGTTCC 90 6.2117397E-7 36.53506 9 GCTTATG 105 5.127731E-8 35.79225 1 GTATATG 105 2.0712469E-6 31.31822 1 AACGCTG 80 2.1464075E-9 29.358532 86-87 GTGACAC 65 0.0061666653 28.909126 1 TAATACT 115 4.1750845E-6 28.624037 4 AATACTG 120 5.8171045E-6 27.429218 5 GTACCCA 225 3.6379788E-12 27.142456 1 TAAGACA 105 7.389473E-5 26.871546 4 CTAAGAC 70 0.008810066 26.871544 3 >>END_MODULE