##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032931_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1250 Sequences flagged as poor quality 0 Sequence length 100 %GC 54 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 14.668 10.0 2.0 28.0 2.0 31.0 2 14.4984 10.0 2.0 28.0 2.0 31.0 3 14.832 10.0 2.0 28.0 2.0 31.0 4 16.332 10.0 2.0 32.0 2.0 35.0 5 16.204 10.0 2.0 32.0 2.0 35.0 6 16.4488 11.0 2.0 32.0 2.0 35.0 7 16.4368 11.0 2.0 32.0 2.0 35.0 8 16.5896 10.0 2.0 32.0 2.0 35.0 9 16.66 10.0 2.0 32.0 2.0 37.0 10-11 16.5852 10.0 2.0 32.5 2.0 37.0 12-13 16.251199999999997 8.0 2.0 32.5 2.0 37.0 14-15 16.4236 2.0 2.0 33.5 2.0 38.5 16-17 16.383200000000002 2.0 2.0 33.0 2.0 38.0 18-19 15.9768 2.0 2.0 33.0 2.0 38.5 20-21 15.9072 2.0 2.0 32.5 2.0 39.0 22-23 15.530000000000001 2.0 2.0 32.5 2.0 38.0 24-25 15.2604 2.0 2.0 32.0 2.0 38.0 26-27 14.835999999999999 2.0 2.0 32.0 2.0 38.0 28-29 14.5852 2.0 2.0 32.0 2.0 38.5 30-31 14.4036 2.0 2.0 32.0 2.0 38.0 32-33 13.923200000000001 2.0 2.0 31.0 2.0 38.0 34-35 13.528 2.0 2.0 30.0 2.0 38.0 36-37 13.3968 2.0 2.0 30.0 2.0 38.0 38-39 13.2936 2.0 2.0 30.0 2.0 38.0 40-41 13.162400000000002 2.0 2.0 30.0 2.0 38.0 42-43 13.011199999999999 2.0 2.0 30.0 2.0 38.0 44-45 13.0076 2.0 2.0 30.0 2.0 38.0 46-47 12.633600000000001 2.0 2.0 30.0 2.0 37.5 48-49 12.3756 2.0 2.0 29.5 2.0 37.0 50-51 12.1988 2.0 2.0 28.5 2.0 37.0 52-53 11.9576 2.0 2.0 28.0 2.0 37.5 54-55 11.766 2.0 2.0 27.5 2.0 37.0 56-57 11.513200000000001 2.0 2.0 27.0 2.0 36.0 58-59 11.3856 2.0 2.0 27.0 2.0 36.5 60-61 11.256 2.0 2.0 26.0 2.0 36.0 62-63 10.9084 2.0 2.0 25.5 2.0 35.5 64-65 10.6728 2.0 2.0 24.0 2.0 35.0 66-67 10.5616 2.0 2.0 24.0 2.0 35.0 68-69 10.311599999999999 2.0 2.0 22.0 2.0 34.0 70-71 10.1872 2.0 2.0 22.5 2.0 34.0 72-73 10.0704 2.0 2.0 21.5 2.0 34.0 74-75 9.8864 2.0 2.0 20.0 2.0 34.0 76-77 9.6172 2.0 2.0 18.0 2.0 34.0 78-79 9.3748 2.0 2.0 14.0 2.0 33.5 80-81 9.27 2.0 2.0 12.5 2.0 33.0 82-83 9.0924 2.0 2.0 7.5 2.0 33.0 84-85 9.069199999999999 2.0 2.0 6.0 2.0 33.0 86-87 8.7208 2.0 2.0 2.0 2.0 33.0 88-89 8.484 2.0 2.0 2.0 2.0 32.5 90-91 8.4268 2.0 2.0 2.0 2.0 32.5 92-93 8.362400000000001 2.0 2.0 2.0 2.0 33.0 94-95 8.1512 2.0 2.0 2.0 2.0 32.5 96-97 7.8956 2.0 2.0 2.0 2.0 33.0 98-99 7.7064 2.0 2.0 2.0 2.0 32.0 100 7.5888 2.0 2.0 2.0 2.0 32.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1102 1 -11.505649717514125 1102 2 -11.121468926553673 1102 3 -11.966101694915254 1102 4 -12.812146892655367 1102 5 -11.81779661016949 1102 6 -12.426553672316382 1102 7 -11.916666666666666 1102 8 -13.132768361581922 1102 9 -13.331920903954803 1102 10-11 -13.25635593220339 1102 12-13 -12.482344632768362 1102 14-15 -12.293785310734464 1102 16-17 -12.756355932203387 1102 18-19 -12.422316384180792 1102 20-21 -12.418079096045199 1102 22-23 -11.853813559322035 1102 24-25 -10.688559322033898 1102 26-27 -10.937146892655369 1102 28-29 -10.561440677966102 1102 30-31 -10.319209039548022 1102 32-33 -10.188559322033898 1102 34-35 -9.331920903954801 1102 36-37 -9.338276836158188 1102 38-39 -9.390536723163843 1102 40-41 -8.985875706214689 1102 42-43 -9.198446327683614 1102 44-45 -8.98446327683616 1102 46-47 -8.253531073446329 1102 48-49 -8.397598870056498 1102 50-51 -8.261299435028247 1102 52-53 -8.0204802259887 1102 54-55 -8.179378531073446 1102 56-57 -7.935028248587569 1102 58-59 -7.448446327683616 1102 60-61 -7.352401129943502 1102 62-63 -6.6610169491525415 1102 64-65 -6.692796610169491 1102 66-67 -6.6574858757062145 1102 68-69 -6.40183615819209 1102 70-71 -6.120056497175142 1102 72-73 -6.197740112994351 1102 74-75 -5.765536723163842 1102 76-77 -5.668079096045198 1102 78-79 -5.82909604519774 1102 80-81 -5.411723163841807 1102 82-83 -5.214689265536723 1102 84-85 -5.065677966101695 1102 86-87 -4.741525423728813 1102 88-89 -4.644067796610169 1102 90-91 -4.771186440677965 1102 92-93 -4.8940677966101696 1102 94-95 -4.975282485875707 1102 96-97 -4.7973163841807915 1102 98-99 -4.18361581920904 1102 100 -4.278248587570622 1103 1 0.4943502824858754 1103 2 0.878531073446327 1103 3 0.03389830508474567 1103 4 1.1878531073446332 1103 5 0.6822033898305104 1103 6 1.5734463276836177 1103 7 -4.416666666666666 1103 8 1.8672316384180778 1103 9 1.6680790960451972 1103 10-11 -0.25635593220338926 1103 12-13 -2.4823446327683616 1103 14-15 1.2062146892655363 1103 16-17 -7.5063559322033875 1103 18-19 -1.1723163841807924 1103 20-21 -4.918079096045199 1103 22-23 0.14618644067796538 1103 24-25 -2.438559322033898 1103 26-27 3.0628531073446315 1103 28-29 3.938559322033898 1103 30-31 -1.5692090395480225 1103 32-33 -6.688559322033898 1103 34-35 -4.581920903954801 1103 36-37 -4.5882768361581885 1103 38-39 2.6094632768361574 1103 40-41 -4.985875706214689 1103 42-43 -2.448446327683614 1103 44-45 2.015536723163841 1103 46-47 5.246468926553671 1103 48-49 3.8524011299435017 1103 50-51 2.7387005649717526 1103 52-53 2.2295197740112993 1103 54-55 5.820621468926554 1103 56-57 6.564971751412431 1103 58-59 1.551553672316384 1103 60-61 4.647598870056498 1103 62-63 0.5889830508474585 1103 64-65 5.307203389830509 1103 66-67 0.09251412429378547 1103 68-69 2.84816384180791 1103 70-71 5.629943502824858 1103 72-73 3.5522598870056488 1103 74-75 3.2344632768361583 1103 76-77 7.331920903954802 1103 78-79 7.17090395480226 1103 80-81 -5.411723163841807 1103 82-83 -5.214689265536723 1103 84-85 -5.065677966101695 1103 86-87 -4.741525423728813 1103 88-89 -4.644067796610169 1103 90-91 -4.771186440677965 1103 92-93 -4.8940677966101696 1103 94-95 -4.975282485875707 1103 96-97 -4.7973163841807915 1103 98-99 -4.18361581920904 1103 100 -4.278248587570622 1104 1 -0.005649717514124575 1104 2 -7.121468926553673 1104 3 -4.966101694915254 1104 4 -5.312146892655367 1104 5 -7.81779661016949 1104 6 -4.926553672316382 1104 7 3.083333333333334 1104 8 -6.132768361581922 1104 9 0.6680790960451972 1104 10-11 1.7436440677966107 1104 12-13 0.5176553672316384 1104 14-15 2.9562146892655363 1104 16-17 1.9936440677966125 1104 18-19 -0.9223163841807924 1104 20-21 1.331920903954801 1104 22-23 -6.103813559322035 1104 24-25 -5.188559322033898 1104 26-27 -3.4371468926553685 1104 28-29 -1.811440677966102 1104 30-31 -2.3192090395480225 1104 32-33 2.311440677966102 1104 34-35 3.918079096045199 1104 36-37 3.6617231638418115 1104 38-39 3.8594632768361574 1104 40-41 -0.7358757062146886 1104 42-43 -6.198446327683614 1104 44-45 -0.4844632768361592 1104 46-47 5.496468926553671 1104 48-49 6.602401129943502 1104 50-51 -8.261299435028247 1104 52-53 -8.0204802259887 1104 54-55 -8.179378531073446 1104 56-57 -7.935028248587569 1104 58-59 -7.448446327683616 1104 60-61 -7.352401129943502 1104 62-63 -6.6610169491525415 1104 64-65 -6.692796610169491 1104 66-67 -6.6574858757062145 1104 68-69 -6.40183615819209 1104 70-71 -6.120056497175142 1104 72-73 -6.197740112994351 1104 74-75 -5.765536723163842 1104 76-77 -5.668079096045198 1104 78-79 -5.82909604519774 1104 80-81 -5.411723163841807 1104 82-83 -5.214689265536723 1104 84-85 -5.065677966101695 1104 86-87 -4.741525423728813 1104 88-89 -4.644067796610169 1104 90-91 -4.771186440677965 1104 92-93 -4.8940677966101696 1104 94-95 -4.975282485875707 1104 96-97 -4.7973163841807915 1104 98-99 -4.18361581920904 1104 100 -4.278248587570622 1105 1 6.161016949152543 1105 2 6.878531073446327 1105 3 6.033898305084746 1105 4 5.521186440677965 1105 5 8.848870056497178 1105 6 6.90677966101695 1105 7 1.75 1105 8 1.5338983050847457 1105 9 -1.3319209039548028 1105 10-11 6.743644067796611 1105 12-13 3.3509887005649706 1105 14-15 2.2062146892655363 1105 16-17 2.410310734463277 1105 18-19 7.077683615819208 1105 20-21 0.9152542372881349 1105 22-23 5.812853107344633 1105 24-25 9.644774011299438 1105 26-27 6.229519774011299 1105 28-29 5.938559322033898 1105 30-31 9.180790960451978 1105 32-33 1.644774011299436 1105 34-35 7.001412429378531 1105 36-37 7.6617231638418115 1105 38-39 9.442796610169493 1105 40-41 10.514124293785311 1105 42-43 8.46822033898305 1105 44-45 2.182203389830507 1105 46-47 2.7464689265536713 1105 48-49 9.102401129943502 1105 50-51 7.738700564971753 1105 52-53 9.9795197740113 1105 54-55 4.820621468926554 1105 56-57 10.06497175141243 1105 58-59 7.551553672316384 1105 60-61 6.980932203389834 1105 62-63 7.3389830508474585 1105 64-65 10.307203389830509 1105 66-67 11.342514124293785 1105 68-69 6.931497175141242 1105 70-71 12.546610169491522 1105 72-73 3.6355932203389827 1105 74-75 -5.765536723163842 1105 76-77 -5.668079096045198 1105 78-79 -5.82909604519774 1105 80-81 -5.411723163841807 1105 82-83 -5.214689265536723 1105 84-85 -5.065677966101695 1105 86-87 -4.741525423728813 1105 88-89 -4.644067796610169 1105 90-91 -4.771186440677965 1105 92-93 -4.8940677966101696 1105 94-95 -4.975282485875707 1105 96-97 -4.7973163841807915 1105 98-99 -4.18361581920904 1105 100 -4.278248587570622 1107 1 0.4943502824858754 1107 2 0.628531073446327 1107 3 0.7838983050847457 1107 4 -3.0621468926553668 1107 5 -6.06779661016949 1107 6 1.3234463276836177 1107 7 -2.666666666666666 1107 8 2.117231638418078 1107 9 -0.5819209039548028 1107 10-11 0.6186440677966107 1107 12-13 0.6426553672316384 1107 14-15 2.9562146892655363 1107 16-17 2.6186440677966125 1107 18-19 2.9526836158192076 1107 20-21 2.706920903954801 1107 22-23 4.146186440677965 1107 24-25 0.5614406779661021 1107 26-27 2.8128531073446315 1107 28-29 1.313559322033898 1107 30-31 -2.8192090395480225 1107 32-33 0.9364406779661021 1107 34-35 6.418079096045199 1107 36-37 4.6617231638418115 1107 38-39 6.484463276836157 1107 40-41 4.764124293785311 1107 42-43 5.176553672316386 1107 44-45 6.390536723163841 1107 46-47 6.496468926553671 1107 48-49 6.852401129943502 1107 50-51 2.4887005649717526 1107 52-53 4.229519774011299 1107 54-55 4.695621468926554 1107 56-57 0.43997175141243083 1107 58-59 5.676553672316384 1107 60-61 8.772598870056498 1107 62-63 8.963983050847459 1107 64-65 9.432203389830509 1107 66-67 7.0925141242937855 1107 68-69 -2.02683615819209 1107 70-71 0.8799435028248581 1107 72-73 -3.4477401129943512 1107 74-75 0.10946327683615831 1107 76-77 1.081920903954802 1107 78-79 0.9209039548022604 1107 80-81 0.21327683615819293 1107 82-83 1.7853107344632768 1107 84-85 2.1843220338983054 1107 86-87 -4.741525423728813 1107 88-89 -4.644067796610169 1107 90-91 -4.771186440677965 1107 92-93 -4.8940677966101696 1107 94-95 -4.975282485875707 1107 96-97 -4.7973163841807915 1107 98-99 -4.18361581920904 1107 100 -4.278248587570622 1108 1 2.4943502824858754 1108 2 -1.121468926553673 1108 3 2.0338983050847457 1108 4 -2.8121468926553668 1108 5 3.6822033898305104 1108 6 4.073446327683618 1108 7 2.083333333333334 1108 8 1.8672316384180778 1108 9 2.168079096045197 1108 10-11 4.243644067796611 1108 12-13 1.5176553672316384 1108 14-15 -3.5437853107344637 1108 16-17 3.9936440677966125 1108 18-19 5.577683615819208 1108 20-21 5.581920903954801 1108 22-23 6.646186440677965 1108 24-25 7.561440677966102 1108 26-27 5.8128531073446315 1108 28-29 5.688559322033898 1108 30-31 6.4307909604519775 1108 32-33 8.061440677966102 1108 34-35 9.168079096045199 1108 36-37 7.1617231638418115 1108 38-39 5.859463276836157 1108 40-41 5.514124293785311 1108 42-43 9.051553672316386 1108 44-45 8.26553672316384 1108 46-47 10.246468926553671 1108 48-49 9.102401129943502 1108 50-51 8.488700564971753 1108 52-53 6.229519774011299 1108 54-55 4.070621468926554 1108 56-57 8.31497175141243 1108 58-59 7.551553672316384 1108 60-61 9.897598870056498 1108 62-63 12.338983050847459 1108 64-65 9.557203389830509 1108 66-67 9.842514124293785 1108 68-69 12.09816384180791 1108 70-71 7.129943502824858 1108 72-73 11.052259887005649 1108 74-75 8.73446327683616 1108 76-77 9.831920903954803 1108 78-79 7.67090395480226 1108 80-81 9.588276836158194 1108 82-83 10.785310734463277 1108 84-85 9.934322033898304 1108 86-87 11.508474576271187 1108 88-89 10.60593220338983 1108 90-91 11.228813559322035 1108 92-93 11.10593220338983 1108 94-95 11.524717514124294 1108 96-97 11.702683615819208 1108 98-99 11.81638418079096 1108 100 12.221751412429377 1109 1 -11.505649717514125 1109 2 -11.121468926553673 1109 3 -11.966101694915254 1109 4 -12.812146892655367 1109 5 -11.81779661016949 1109 6 -12.426553672316382 1109 7 -11.916666666666666 1109 8 -13.132768361581922 1109 9 -13.331920903954803 1109 10-11 -13.25635593220339 1109 12-13 -12.482344632768362 1109 14-15 -12.293785310734464 1109 16-17 -12.756355932203387 1109 18-19 -12.422316384180792 1109 20-21 -12.418079096045199 1109 22-23 -11.853813559322035 1109 24-25 -10.688559322033898 1109 26-27 -10.937146892655369 1109 28-29 -10.561440677966102 1109 30-31 -10.319209039548022 1109 32-33 -10.188559322033898 1109 34-35 -9.331920903954801 1109 36-37 -9.338276836158188 1109 38-39 -9.390536723163843 1109 40-41 -8.985875706214689 1109 42-43 -9.198446327683614 1109 44-45 -8.98446327683616 1109 46-47 -8.253531073446329 1109 48-49 -8.397598870056498 1109 50-51 -8.261299435028247 1109 52-53 -8.0204802259887 1109 54-55 -8.179378531073446 1109 56-57 -7.935028248587569 1109 58-59 -7.448446327683616 1109 60-61 -7.352401129943502 1109 62-63 -6.6610169491525415 1109 64-65 -6.692796610169491 1109 66-67 -6.6574858757062145 1109 68-69 -6.40183615819209 1109 70-71 -6.120056497175142 1109 72-73 -6.197740112994351 1109 74-75 -5.765536723163842 1109 76-77 -5.668079096045198 1109 78-79 -5.82909604519774 1109 80-81 -5.411723163841807 1109 82-83 -5.214689265536723 1109 84-85 -5.065677966101695 1109 86-87 -4.741525423728813 1109 88-89 -4.644067796610169 1109 90-91 -4.771186440677965 1109 92-93 -4.8940677966101696 1109 94-95 -4.975282485875707 1109 96-97 -4.7973163841807915 1109 98-99 -4.18361581920904 1109 100 -4.278248587570622 1110 1 2.4943502824858754 1110 2 2.878531073446327 1110 3 2.0338983050847457 1110 4 3.6878531073446332 1110 5 4.68220338983051 1110 6 4.073446327683618 1110 7 1.083333333333334 1110 8 3.367231638418078 1110 9 3.168079096045197 1110 10-11 5.243644067796611 1110 12-13 5.517655367231638 1110 14-15 6.956214689265536 1110 16-17 6.7436440677966125 1110 18-19 7.077683615819208 1110 20-21 5.331920903954801 1110 22-23 6.896186440677965 1110 24-25 8.561440677966102 1110 26-27 8.562853107344631 1110 28-29 8.938559322033898 1110 30-31 9.180790960451978 1110 32-33 9.311440677966102 1110 34-35 10.168079096045199 1110 36-37 9.161723163841812 1110 38-39 10.109463276836157 1110 40-41 10.514124293785311 1110 42-43 10.051553672316386 1110 44-45 10.51553672316384 1110 46-47 10.996468926553671 1110 48-49 10.602401129943502 1110 50-51 9.238700564971753 1110 52-53 9.4795197740113 1110 54-55 10.320621468926554 1110 56-57 11.06497175141243 1110 58-59 9.801553672316384 1110 60-61 10.647598870056498 1110 62-63 10.088983050847459 1110 64-65 10.557203389830509 1110 66-67 10.842514124293785 1110 68-69 11.09816384180791 1110 70-71 10.629943502824858 1110 72-73 10.302259887005649 1110 74-75 10.23446327683616 1110 76-77 10.331920903954803 1110 78-79 10.42090395480226 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-9.390536723163843 2213 40-41 -8.985875706214689 2213 42-43 -9.198446327683614 2213 44-45 -8.98446327683616 2213 46-47 -8.253531073446329 2213 48-49 -8.397598870056498 2213 50-51 -8.261299435028247 2213 52-53 -8.0204802259887 2213 54-55 -8.179378531073446 2213 56-57 -7.935028248587569 2213 58-59 -7.448446327683616 2213 60-61 -7.352401129943502 2213 62-63 -6.6610169491525415 2213 64-65 -6.692796610169491 2213 66-67 -6.6574858757062145 2213 68-69 -6.40183615819209 2213 70-71 -6.120056497175142 2213 72-73 -6.197740112994351 2213 74-75 -5.765536723163842 2213 76-77 -5.668079096045198 2213 78-79 -5.82909604519774 2213 80-81 -5.411723163841807 2213 82-83 -5.214689265536723 2213 84-85 -5.065677966101695 2213 86-87 -4.741525423728813 2213 88-89 -4.644067796610169 2213 90-91 -4.771186440677965 2213 92-93 -4.8940677966101696 2213 94-95 -4.975282485875707 2213 96-97 -4.7973163841807915 2213 98-99 -4.18361581920904 2213 100 -4.278248587570622 2214 1 -6.005649717514125 2214 2 -3.371468926553673 2214 3 0.2838983050847457 2214 4 2.1878531073446332 2214 5 -6.31779661016949 2214 6 -5.426553672316382 2214 7 3.083333333333334 2214 8 -1.3827683615819222 2214 9 1.9180790960451972 2214 10-11 0.7436440677966107 2214 12-13 -1.6073446327683616 2214 14-15 -0.9187853107344637 2214 16-17 -3.1313559322033875 2214 18-19 -5.172316384180792 2214 20-21 1.706920903954801 2214 22-23 -1.8538135593220346 2214 24-25 0.3114406779661021 2214 26-27 1.5628531073446315 2214 28-29 1.188559322033898 2214 30-31 1.9307909604519775 2214 32-33 4.436440677966102 2214 34-35 0.918079096045199 2214 36-37 2.6617231638418115 2214 38-39 2.3594632768361574 2214 40-41 -2.1108757062146886 2214 42-43 4.301553672316386 2214 44-45 -0.9844632768361592 2214 46-47 -3.8785310734463287 2214 48-49 -2.0225988700564983 2214 50-51 -1.3862994350282474 2214 52-53 -5.020480225988701 2214 54-55 -5.679378531073446 2214 56-57 -3.185028248587569 2214 58-59 -3.823446327683616 2214 60-61 -7.352401129943502 2214 62-63 -6.6610169491525415 2214 64-65 -6.692796610169491 2214 66-67 -6.6574858757062145 2214 68-69 -6.40183615819209 2214 70-71 -6.120056497175142 2214 72-73 -6.197740112994351 2214 74-75 -5.765536723163842 2214 76-77 -5.668079096045198 2214 78-79 -5.82909604519774 2214 80-81 -5.411723163841807 2214 82-83 -5.214689265536723 2214 84-85 -5.065677966101695 2214 86-87 -4.741525423728813 2214 88-89 -4.644067796610169 2214 90-91 -4.771186440677965 2214 92-93 -4.8940677966101696 2214 94-95 -4.975282485875707 2214 96-97 -4.7973163841807915 2214 98-99 -4.18361581920904 2214 100 -4.278248587570622 2215 1 -11.505649717514125 2215 2 -11.121468926553673 2215 3 -11.966101694915254 2215 4 -12.812146892655367 2215 5 -11.81779661016949 2215 6 -12.426553672316382 2215 7 -11.916666666666666 2215 8 -13.132768361581922 2215 9 -13.331920903954803 2215 10-11 -13.25635593220339 2215 12-13 -12.482344632768362 2215 14-15 -12.293785310734464 2215 16-17 -12.756355932203387 2215 18-19 -12.422316384180792 2215 20-21 -12.418079096045199 2215 22-23 -11.853813559322035 2215 24-25 -10.688559322033898 2215 26-27 -10.937146892655369 2215 28-29 -10.561440677966102 2215 30-31 -10.319209039548022 2215 32-33 -10.188559322033898 2215 34-35 -9.331920903954801 2215 36-37 -9.338276836158188 2215 38-39 -9.390536723163843 2215 40-41 -8.985875706214689 2215 42-43 -9.198446327683614 2215 44-45 -8.98446327683616 2215 46-47 -8.253531073446329 2215 48-49 -8.397598870056498 2215 50-51 -8.261299435028247 2215 52-53 -8.0204802259887 2215 54-55 -8.179378531073446 2215 56-57 -7.935028248587569 2215 58-59 -7.448446327683616 2215 60-61 -7.352401129943502 2215 62-63 -6.6610169491525415 2215 64-65 -6.692796610169491 2215 66-67 -6.6574858757062145 2215 68-69 -6.40183615819209 2215 70-71 -6.120056497175142 2215 72-73 -6.197740112994351 2215 74-75 -5.765536723163842 2215 76-77 -5.668079096045198 2215 78-79 -5.82909604519774 2215 80-81 -5.411723163841807 2215 82-83 -5.214689265536723 2215 84-85 -5.065677966101695 2215 86-87 -4.741525423728813 2215 88-89 -4.644067796610169 2215 90-91 -4.771186440677965 2215 92-93 -4.8940677966101696 2215 94-95 -4.975282485875707 2215 96-97 -4.7973163841807915 2215 98-99 -4.18361581920904 2215 100 -4.278248587570622 2216 1 0.7443502824858754 2216 2 3.628531073446327 2216 3 5.033898305084746 2216 4 -0.5621468926553668 2216 5 4.18220338983051 2216 6 9.323446327683618 2216 7 10.833333333333334 2216 8 10.867231638418078 2216 9 11.168079096045197 2216 10-11 10.86864406779661 2216 12-13 8.767655367231638 2216 14-15 5.456214689265536 2216 16-17 9.118644067796613 2216 18-19 9.452683615819208 2216 20-21 10.581920903954801 2216 22-23 10.771186440677965 2216 24-25 6.311440677966102 2216 26-27 10.187853107344631 2216 28-29 11.063559322033898 2216 30-31 4.8057909604519775 2216 32-33 7.436440677966102 2216 34-35 5.668079096045199 2216 36-37 3.4117231638418115 2216 38-39 -0.14053672316384258 2216 40-41 -0.48587570621468856 2216 42-43 0.30155367231638586 2216 44-45 -0.2344632768361592 2216 46-47 -2.6285310734463287 2216 48-49 -1.1475988700564983 2216 50-51 -1.0112994350282474 2216 52-53 -0.3954802259887007 2216 54-55 -0.05437853107344637 2216 56-57 -0.31002824858756917 2216 58-59 -0.5734463276836159 2216 60-61 -1.3524011299435017 2216 62-63 0.9639830508474585 2216 64-65 1.3072033898305087 2216 66-67 -1.5324858757062145 2216 68-69 -2.40183615819209 2216 70-71 -0.3700564971751419 2216 72-73 0.8022598870056488 2216 74-75 1.3594632768361583 2216 76-77 2.331920903954802 2216 78-79 0.6709039548022604 2216 80-81 1.588276836158193 2216 82-83 1.0353107344632768 2216 84-85 -0.19067796610169463 2216 86-87 2.258474576271187 2216 88-89 3.3559322033898313 2216 90-91 3.2288135593220346 2216 92-93 1.3559322033898304 2216 94-95 2.3997175141242932 2216 96-97 1.3276836158192085 2216 98-99 2.06638418079096 2216 100 1.471751412429378 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 561.0 3 48.0 4 18.0 5 26.0 6 27.0 7 23.0 8 23.0 9 21.0 10 11.0 11 20.0 12 23.0 13 25.0 14 21.0 15 9.0 16 10.0 17 11.0 18 16.0 19 8.0 20 9.0 21 13.0 22 15.0 23 10.0 24 13.0 25 17.0 26 18.0 27 9.0 28 13.0 29 13.0 30 23.0 31 25.0 32 21.0 33 23.0 34 28.0 35 24.0 36 30.0 37 26.0 38 16.0 39 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.834267413931144 19.615692554043235 13.210568454763811 24.339471577261808 2 22.257806244995997 23.698959167333868 29.943955164131303 24.09927942353883 3 20.656525220176142 27.702161729383505 25.86068855084067 25.780624499599682 4 19.135308246597276 19.135308246597276 29.22337870296237 32.506004803843076 5 17.854283426741393 37.55004003202562 26.82145716573259 17.7742193755004 6 32.746196957566056 34.4275420336269 16.653322658126502 16.172938350680543 7 28.422738190552444 32.345876701361085 20.25620496397118 18.975180144115292 8 27.061649319455565 36.269015212169734 15.772618094475579 20.89671737389912 9 28.102481985588472 19.135308246597276 18.094475580464373 34.66773418734988 10-11 26.54123298638911 26.621297037630104 24.259407526020816 22.57806244995997 12-13 25.500400320256205 25.540432345876702 24.259407526020816 24.699759807846277 14-15 25.260208166533225 24.85988791032826 23.618895116092876 26.261008807045638 16-17 28.502802241793436 28.022417934347477 19.935948759007204 23.53883106485188 18-19 27.061649319455565 26.861489191353083 22.938350680544435 23.138510808646917 20-21 26.020816653322658 26.060848678943156 24.619695756605285 23.2986389111289 22-23 30.704563650920736 24.699759807846277 21.617293835068054 22.978382706164933 24-25 27.822257806244995 24.179343474779824 21.857485988791034 26.140912730184144 26-27 26.82145716573259 23.218574859887912 23.77902321857486 26.180944755804642 28-29 28.943154523618897 23.698959167333868 19.815852682145714 27.542033626901517 30-31 24.419535628502803 25.22017614091273 22.33787029623699 28.022417934347477 32-33 27.18174539631705 22.81825460368295 19.45556445156125 30.544435548438752 34-35 31.184947958366692 21.056845476381106 19.255404323458766 28.502802241793436 36-37 29.78382706164932 21.377101681345074 21.0968775020016 27.742193755004003 38-39 25.86068855084067 22.41793434747798 22.81825460368295 28.903122497998403 40-41 26.54123298638911 21.417133706965572 20.936749399519616 31.104883907125704 42-43 23.939151321056844 23.2986389111289 22.497998398718973 30.26421136909528 44-45 26.461168935148116 22.738190552441953 23.618895116092876 27.18174539631705 46-47 26.701361088871096 24.459567654123298 22.2177742193755 26.621297037630104 48-49 27.22177742193755 22.53803042433947 21.497197758206564 28.742994395516412 50-51 25.90072057646117 22.297838270616495 22.377902321857484 29.42353883106485 52-53 28.622898318654922 25.580464371497197 20.97678142514011 24.819855884707767 54-55 26.861489191353083 25.940752602081663 20.37630104083267 26.82145716573259 56-57 25.420336269015216 24.699759807846277 22.65812650120096 27.22177742193755 58-59 29.943955164131303 23.018414731785427 21.0968775020016 25.940752602081663 60-61 30.66453162530024 22.177742193755005 21.497197758206564 25.66052842273819 62-63 30.704563650920736 23.65892714171337 20.816653322658127 24.819855884707767 64-65 28.502802241793436 24.89991993594876 20.056044835868693 26.54123298638911 66-67 29.343474779823858 22.69815852682146 21.33706965572458 26.621297037630104 68-69 30.184147317854283 21.977582065652523 19.77582065652522 28.06244995996797 70-71 27.702161729383505 24.01921537229784 21.01681345076061 27.261809447558043 72-73 28.382706164931946 22.65812650120096 21.497197758206564 27.46196957566053 74-75 30.904723779023218 22.017614091273018 21.33706965572458 25.740592473979184 76-77 32.02562049639712 22.45796637309848 21.056845476381106 24.459567654123298 78-79 31.585268214571656 22.938350680544435 21.57726180944756 23.89911929543635 80-81 26.941553242594075 24.219375500400318 22.778222578062447 26.060848678943156 82-83 32.90632506004803 20.936749399519616 20.25620496397118 25.90072057646117 84-85 30.544435548438752 24.09927942353883 20.776621297037632 24.579663730984787 86-87 28.022417934347477 21.737389911929544 21.297037630104082 28.943154523618897 88-89 31.385108086469177 22.45796637309848 20.25620496397118 25.90072057646117 90-91 29.063250600480384 22.2177742193755 23.498799039231386 25.22017614091273 92-93 29.183346677341877 22.778222578062447 22.45796637309848 25.580464371497197 94-95 30.064051240992796 21.77742193755004 21.176941553242596 26.98158526821457 96-97 28.34267413931145 22.57806244995997 20.496397117694155 28.582866293034424 98-99 31.265012009607684 21.176941553242596 22.09767814251401 25.46036829463571 100 29.943955164131303 25.380304243394715 19.055244195356284 25.62049639711769 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4.0 1 2.5 2 1.0 3 0.5 4 0.0 5 0.0 6 0.0 7 0.5 8 0.5 9 0.0 10 0.5 11 0.5 12 0.0 13 0.5 14 0.5 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.5 22 2.0 23 3.0 24 2.0 25 2.5 26 4.5 27 3.0 28 4.0 29 7.0 30 6.5 31 5.5 32 8.0 33 12.5 34 14.0 35 14.5 36 11.5 37 13.5 38 18.5 39 17.5 40 19.5 41 23.0 42 23.0 43 26.0 44 31.0 45 29.0 46 29.0 47 30.0 48 31.0 49 34.0 50 35.0 51 34.5 52 32.0 53 38.0 54 41.5 55 42.0 56 35.5 57 34.5 58 34.5 59 32.0 60 36.5 61 31.5 62 30.5 63 29.5 64 28.0 65 28.0 66 27.0 67 27.0 68 23.0 69 16.5 70 14.5 71 12.0 72 12.5 73 14.0 74 13.5 75 14.0 76 9.0 77 7.5 78 9.5 79 8.0 80 7.0 81 6.5 82 8.5 83 8.5 84 5.5 85 4.5 86 5.5 87 4.5 88 2.0 89 1.5 90 3.5 91 4.5 92 1.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08 2 0.08 3 0.08 4 0.08 5 0.08 6 0.08 7 0.08 8 0.08 9 0.08 10-11 0.08 12-13 0.08 14-15 0.08 16-17 0.08 18-19 0.08 20-21 0.08 22-23 0.08 24-25 0.08 26-27 0.08 28-29 0.08 30-31 0.08 32-33 0.08 34-35 0.08 36-37 0.08 38-39 0.08 40-41 0.08 42-43 0.08 44-45 0.08 46-47 0.08 48-49 0.08 50-51 0.08 52-53 0.08 54-55 0.08 56-57 0.08 58-59 0.08 60-61 0.08 62-63 0.08 64-65 0.08 66-67 0.08 68-69 0.08 70-71 0.08 72-73 0.08 74-75 0.08 76-77 0.08 78-79 0.08 80-81 0.08 82-83 0.08 84-85 0.08 86-87 0.08 88-89 0.08 90-91 0.08 92-93 0.08 94-95 0.08 96-97 0.08 98-99 0.08 100 0.08 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1250.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.68 #Duplication Level Percentage of deduplicated Percentage of total 1 99.91974317817014 99.6 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.08025682182985554 0.4 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5 0.4 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.08 0.0 40-41 0.0 0.0 0.0 0.16 0.0 42-43 0.0 0.0 0.0 0.24 0.0 44-45 0.0 0.0 0.0 0.24 0.0 46-47 0.0 0.0 0.0 0.32 0.0 48-49 0.0 0.0 0.0 0.36 0.0 50-51 0.0 0.0 0.0 0.4 0.0 52-53 0.0 0.0 0.0 0.4 0.0 54-55 0.0 0.0 0.0 0.44 0.0 56-57 0.0 0.0 0.0 0.52 0.0 58-59 0.0 0.0 0.0 0.64 0.0 60-61 0.0 0.0 0.0 0.76 0.0 62-63 0.0 0.0 0.0 0.88 0.0 64-65 0.0 0.0 0.0 0.96 0.0 66-67 0.0 0.0 0.0 1.04 0.0 68-69 0.0 0.0 0.0 1.2 0.0 70-71 0.0 0.0 0.0 1.2 0.0 72-73 0.0 0.0 0.0 1.32 0.0 74-75 0.0 0.0 0.0 1.52 0.0 76-77 0.0 0.0 0.0 1.52 0.0 78-79 0.0 0.0 0.0 1.76 0.0 80-81 0.0 0.0 0.0 1.8 0.0 82-83 0.0 0.0 0.0 1.96 0.0 84-85 0.0 0.0 0.0 2.08 0.0 86-87 0.0 0.0 0.0 2.32 0.0 88 0.0 0.0 0.0 2.4 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE