FastQCFastQC Report
Wed 25 May 2016
SRR1032930_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032930_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2750377
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39650.036.9841161
TCAACGC40200.035.5421264
ATCAACG40500.035.3955463
CAACGCA40900.035.0487445
AACGCAG42700.033.351146
TATCAAC46300.030.961552
GGTATCA23950.030.8104461
GTACATG92050.029.8189871
TACATGG92000.028.762352
ACATGGG94000.027.0503713
ACGCAGA52400.026.907797
CGCAGAG54600.025.4788138
TACACCG3750.025.0665555
CATGGGG59650.023.7165764
ACACCGT5300.023.0565016
ACCGTTC3400.022.1167438
GCAGAGT65050.020.6625089
GAGTACA65000.020.2464331
TAGCGCG1200.005066021319.5832464
GTACACG3255.620677E-1018.8002591
Produced by FastQC (version 0.11.5)