##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032930_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2750377 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.753109119222565 33.0 31.0 34.0 30.0 34.0 2 31.876963412652156 34.0 31.0 34.0 30.0 34.0 3 32.04641109200666 34.0 31.0 34.0 30.0 34.0 4 35.36134500833886 37.0 35.0 37.0 33.0 37.0 5 35.45611419816265 37.0 35.0 37.0 35.0 37.0 6 35.504186153389156 37.0 37.0 37.0 35.0 37.0 7 35.42445272048159 37.0 37.0 37.0 35.0 37.0 8 35.4480396687436 37.0 37.0 37.0 35.0 37.0 9 37.123527065562286 39.0 38.0 39.0 35.0 39.0 10-11 37.12269517960628 39.0 38.5 39.0 35.0 39.0 12-13 37.079679440309455 39.0 38.0 39.0 35.0 39.0 14-15 38.59815799797627 41.0 39.5 41.0 36.0 41.0 16-17 38.632206421156084 41.0 40.0 41.0 36.0 41.0 18-19 38.648218953256226 41.0 40.0 41.0 35.0 41.0 20-21 38.693303499847474 41.0 40.0 41.0 35.0 41.0 22-23 38.692202378073986 41.0 39.5 41.0 35.0 41.0 24-25 38.727837311030456 41.0 39.0 41.0 35.0 41.0 26-27 38.709257130931505 41.0 39.0 41.0 35.0 41.0 28-29 38.69874766259316 41.0 39.0 41.0 35.0 41.0 30-31 38.66164493085857 41.0 39.0 41.0 35.0 41.0 32-33 38.612389319718716 41.0 39.0 41.0 35.0 41.0 34-35 38.54958283900716 41.0 39.0 41.0 34.5 41.0 36-37 38.46784168134042 41.0 39.0 41.0 34.0 41.0 38-39 38.379220012383755 41.0 39.0 41.0 34.0 41.0 40-41 38.29684730493311 41.0 39.0 41.0 34.0 41.0 42-43 38.19545829535369 41.0 38.5 41.0 33.0 41.0 44-45 38.072225916665246 40.0 38.0 41.0 33.0 41.0 46-47 37.95956899726838 40.0 38.0 41.0 33.0 41.0 48-49 37.83812928191299 40.0 38.0 41.0 33.0 41.0 50-51 37.70883282546356 40.0 38.0 41.0 33.0 41.0 52-53 37.562739944378535 40.0 37.5 41.0 32.5 41.0 54-55 37.408969206766926 40.0 37.0 41.0 32.0 41.0 56-57 37.23703986762542 40.0 37.0 41.0 32.0 41.0 58-59 37.039177174620065 40.0 36.0 41.0 31.5 41.0 60-61 36.807314960821735 39.5 36.0 41.0 31.0 41.0 62-63 36.56372708177825 39.0 35.0 41.0 31.0 41.0 64-65 36.28501638139062 39.0 35.0 41.0 31.0 41.0 66-67 35.990506574189645 38.0 35.0 40.5 30.5 41.0 68-69 35.67435609736411 37.5 35.0 40.0 30.0 41.0 70-71 35.33059395130195 37.0 35.0 40.0 30.0 41.0 72-73 35.03284676973375 36.5 35.0 39.0 30.0 41.0 74-75 34.748313049447404 36.0 35.0 39.0 29.5 41.0 76-77 34.4157697290226 36.0 34.0 39.0 29.0 40.5 78-79 34.04076077570457 35.0 34.0 37.5 29.0 39.5 80-81 33.711873681317144 35.0 34.0 37.0 29.0 39.0 82-83 33.38841311572922 35.0 34.0 37.0 29.0 39.0 84-85 33.21348546035689 35.0 34.0 36.0 29.0 38.0 86-87 32.977603434001956 35.0 34.0 36.0 29.0 37.0 88-89 32.74116348413327 35.0 34.0 36.0 29.0 37.0 90-91 32.53786244576653 35.0 34.0 35.0 28.0 36.5 92-93 32.348561306322736 35.0 34.0 35.0 28.0 36.0 94-95 32.175612106994784 35.0 34.0 35.0 27.0 36.0 96-97 32.01056255197015 35.0 34.0 35.0 27.0 36.0 98-99 31.84012628086986 35.0 34.0 35.0 27.0 35.5 100 31.719139957903952 35.0 34.0 35.0 27.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.32039806849178376 1101 2 0.37057445227388897 1101 3 0.34641651502342086 1101 4 0.3040877686183805 1101 5 0.29310339782743 1101 6 0.2498801783547222 1101 7 0.34934625301850275 1101 8 0.26587955851232437 1101 9 0.33313166002522365 1101 10-11 0.3051326904732363 1101 12-13 0.14513130129348895 1101 14-15 0.16012916170279112 1101 16-17 0.19969072487795358 1101 18-19 0.22775917354177722 1101 20-21 0.18858599226042827 1101 22-23 0.2277912072345103 1101 24-25 0.23857001539231248 1101 26-27 0.24006563694063487 1101 28-29 0.2711153264976147 1101 30-31 0.2703319206930175 1101 32-33 0.27171149371152836 1101 34-35 0.2892757326259172 1101 36-37 0.3231594495981298 1101 38-39 0.32107878797948786 1101 40-41 0.3633405444187616 1101 42-43 0.30318282032365573 1101 44-45 0.3223104348713619 1101 46-47 0.28087001175479287 1101 48-49 0.2909087428251951 1101 50-51 0.3415467860447947 1101 52-53 0.30701131738172194 1101 54-55 0.26874091529099076 1101 56-57 0.2743976666911081 1101 58-59 0.2038220545140561 1101 60-61 0.26469750338888787 1101 62-63 0.25700527481753 1101 64-65 0.2778028301370128 1101 66-67 0.2710693037074776 1101 68-69 0.29667611358716783 1101 70-71 0.2901728408776947 1101 72-73 0.2689483069972809 1101 74-75 0.30863058826551537 1101 76-77 0.27733823883060893 1101 78-79 0.3402897940135716 1101 80-81 0.34790456502710754 1101 82-83 0.34476268589245507 1101 84-85 0.27067625302130693 1101 86-87 0.2688699340332903 1101 88-89 0.29982112936983185 1101 90-91 0.31585026158010976 1101 92-93 0.24476530836806631 1101 94-95 0.2785543944080189 1101 96-97 0.28276953831949214 1101 98-99 0.33756044599054036 1101 100 0.3739509009468023 1102 1 0.2264387354489017 1102 2 0.17382757408115523 1102 3 0.3194057211329735 1102 4 0.26492755969808 1102 5 0.20554179935722772 1102 6 0.24890720988088333 1102 7 0.27495414298869036 1102 8 0.23091222250839394 1102 9 0.3427079869438501 1102 10-11 0.2849940723826876 1102 12-13 0.31107707695630893 1102 14-15 0.32624555937334065 1102 16-17 0.34633349963616666 1102 18-19 0.34050731670785694 1102 20-21 0.2918851366579389 1102 22-23 0.2788400771581152 1102 24-25 0.3267498089903711 1102 26-27 0.3720101470203332 1102 28-29 0.3499509641109526 1102 30-31 0.3306861498169127 1102 32-33 0.39629631476307736 1102 34-35 0.34489892636501907 1102 36-37 0.3640293466776612 1102 38-39 0.40340910883270453 1102 40-41 0.3808876287756249 1102 42-43 0.397340024589262 1102 44-45 0.3757718321470733 1102 46-47 0.33100407027041 1102 48-49 0.3529682392566542 1102 50-51 0.3851540668521025 1102 52-53 0.40641054650714636 1102 54-55 0.3370280197276614 1102 56-57 0.3584284147815282 1102 58-59 0.4107081559966943 1102 60-61 0.3970210252771409 1102 62-63 0.3751117569751301 1102 64-65 0.371285092308149 1102 66-67 0.34133039463341674 1102 68-69 0.325109011613975 1102 70-71 0.3187406129910286 1102 72-73 0.2775739221435387 1102 74-75 0.2685000786266869 1102 76-77 0.23889561797900427 1102 78-79 0.2654538838318672 1102 80-81 0.26657183221819736 1102 82-83 0.2291608448668896 1102 84-85 0.24533360652876723 1102 86-87 0.20231908097660067 1102 88-89 0.23740941301533525 1102 90-91 0.21602476326627595 1102 92-93 0.22252320332314213 1102 94-95 0.24885050152800403 1102 96-97 0.16215269841133306 1102 98-99 0.18307313673123105 1102 100 0.125697061132243 1103 1 0.17409889128147782 1103 2 0.21883138314316497 1103 3 0.28870629002184245 1103 4 0.2419789665231349 1103 5 0.21375311439710032 1103 6 0.22560212756952325 1103 7 0.31269602991642387 1103 8 0.2625383530184635 1103 9 0.3431839760647577 1103 10-11 0.3177426696941694 1103 12-13 0.31166840079781366 1103 14-15 0.3625946682366745 1103 16-17 0.3207083653423126 1103 18-19 0.35499870064407446 1103 20-21 0.30869685983622475 1103 22-23 0.3136207487250431 1103 24-25 0.328822099586759 1103 26-27 0.3557460842276825 1103 28-29 0.38439769641834687 1103 30-31 0.4229000651304915 1103 32-33 0.3974745099226311 1103 34-35 0.41456468835551874 1103 36-37 0.4039015506365544 1103 38-39 0.41258647783372737 1103 40-41 0.40573012434894906 1103 42-43 0.42227185789138133 1103 44-45 0.4395147325266038 1103 46-47 0.4425157967809028 1103 48-49 0.37934498823659624 1103 50-51 0.3977024270020806 1103 52-53 0.34737180897852227 1103 54-55 0.3789811320702867 1103 56-57 0.35614240305903877 1103 58-59 0.3592388519806846 1103 60-61 0.3286877168396387 1103 62-63 0.30335531977524965 1103 64-65 0.3073349341430287 1103 66-67 0.33539015381796844 1103 68-69 0.3723324754359254 1103 70-71 0.3323604513673004 1103 72-73 0.32076713408481083 1103 74-75 0.3879826839101952 1103 76-77 0.3675715351701143 1103 78-79 0.4153406173802239 1103 80-81 0.294478525711078 1103 82-83 0.2653898792265821 1103 84-85 0.2332290624269504 1103 86-87 0.2726599029756329 1103 88-89 0.22585741271198856 1103 90-91 0.176777492519534 1103 92-93 0.24790761409055762 1103 94-95 0.28323360583885204 1103 96-97 0.268017188876037 1103 98-99 0.3382634002401872 1103 100 0.3489253302324187 1104 1 0.02969877218305328 1104 2 0.09068436884890829 1104 3 0.07148436949994164 1104 4 0.030138344342518053 1104 5 0.11215827127206524 1104 6 0.1177878961224863 1104 7 0.0681381135521022 1104 8 0.1600205921666884 1104 9 0.12088672116939847 1104 10-11 0.16497727159055842 1104 12-13 0.11972983483089905 1104 14-15 0.20572748525949436 1104 16-17 0.18237614674562508 1104 18-19 0.18107822030577125 1104 20-21 0.20090093189404 1104 22-23 0.20509512558409426 1104 24-25 0.2386730594782449 1104 26-27 0.2084214793456738 1104 28-29 0.23288301517371224 1104 30-31 0.2271017008865357 1104 32-33 0.216053090298729 1104 34-35 0.24297289604101024 1104 36-37 0.23445038547414043 1104 38-39 0.27840721921599254 1104 40-41 0.29055173750781194 1104 42-43 0.3021399449734048 1104 44-45 0.22964752700113422 1104 46-47 0.20243965827573618 1104 48-49 0.2562679089516564 1104 50-51 0.25758254345991105 1104 52-53 0.2898549636415737 1104 54-55 0.37477237866190194 1104 56-57 0.37518955995084013 1104 58-59 0.29472110281056274 1104 60-61 0.3757168916076097 1104 62-63 0.3442255519329649 1104 64-65 0.37818121349892664 1104 66-67 0.3916472833557094 1104 68-69 0.40730884320013416 1104 70-71 0.41262705906430597 1104 72-73 0.3601751573090013 1104 74-75 0.3475206300980318 1104 76-77 0.3684610719223258 1104 78-79 0.3882491210459449 1104 80-81 0.4066425052828748 1104 82-83 0.4150993391027171 1104 84-85 0.39700498650605454 1104 86-87 0.3534838337181583 1104 88-89 0.3665609709133264 1104 90-91 0.38357826671364137 1104 92-93 0.4090456941011169 1104 94-95 0.4106255920686408 1104 96-97 0.42485897107098936 1104 98-99 0.4704243154181498 1104 100 0.4229957770607058 1105 1 0.09840764606665786 1105 2 0.1281780596006179 1105 3 0.21637048853502705 1105 4 0.2283238844476827 1105 5 0.20676077702965046 1105 6 0.261028841610063 1105 7 0.25341405519613147 1105 8 0.2795110523770532 1105 9 0.2793083367184792 1105 10-11 0.2848561469125954 1105 12-13 0.31841724695898677 1105 14-15 0.37478946437594374 1105 16-17 0.33970530854233516 1105 18-19 0.3560882609685265 1105 20-21 0.3254562233361895 1105 22-23 0.3844323825523617 1105 24-25 0.3758492319930724 1105 26-27 0.3926133774201901 1105 28-29 0.39277995724411596 1105 30-31 0.396395233926377 1105 32-33 0.41248115997035484 1105 34-35 0.42936355747707466 1105 36-37 0.43653003357920994 1105 38-39 0.4304842057076286 1105 40-41 0.45062680789556 1105 42-43 0.48056809662015354 1105 44-45 0.45762473817676863 1105 46-47 0.5004197514695861 1105 48-49 0.47597581588978244 1105 50-51 0.4615579627832531 1105 52-53 0.4794722030083989 1105 54-55 0.43596296797017686 1105 56-57 0.49364443986760875 1105 58-59 0.3972992653099894 1105 60-61 0.37119366326257364 1105 62-63 0.38272736904802684 1105 64-65 0.42859871302074026 1105 66-67 0.444924825061193 1105 68-69 0.4953308481052403 1105 70-71 0.31942737825168876 1105 72-73 0.3289324880239448 1105 74-75 0.33287801840177167 1105 76-77 0.27641284503376795 1105 78-79 0.23498584765069097 1105 80-81 0.2877850966142361 1105 82-83 0.2876359288760426 1105 84-85 0.2546172717918225 1105 86-87 0.2748313525273218 1105 88-89 0.3368005238550751 1105 90-91 0.353815306267407 1105 92-93 0.2659389248641659 1105 94-95 0.24940261227833815 1105 96-97 0.26587496884992845 1105 98-99 0.26803767952738156 1105 100 0.23324703594585472 1106 1 0.07519595174204596 1106 2 0.17806354485684395 1106 3 0.12334848393469144 1106 4 0.17103936466921255 1106 5 0.12199173154952803 1106 6 0.1702403681113438 1106 7 0.1653549320236749 1106 8 0.1160703779247001 1106 9 0.17857262884954395 1106 10-11 0.20426713637921523 1106 12-13 0.25438314427085373 1106 14-15 0.2808398656711475 1106 16-17 0.23929154999075308 1106 18-19 0.17723992390029508 1106 20-21 0.18501980863486978 1106 22-23 0.2411018791810875 1106 24-25 0.23772433688264982 1106 26-27 0.2526013336281778 1106 28-29 0.2154196948474052 1106 30-31 0.17809471663564125 1106 32-33 0.1861137700180393 1106 34-35 0.17280018442555445 1106 36-37 0.2160125207209731 1106 38-39 0.20419492764411729 1106 40-41 0.22633030849281255 1106 42-43 0.22862443096371976 1106 44-45 0.18041625717535226 1106 46-47 0.18423257415930294 1106 48-49 0.22339269398094075 1106 50-51 0.3018604890179901 1106 52-53 0.31616319583147856 1106 54-55 0.31074516783789363 1106 56-57 0.3142403114136556 1106 58-59 0.2590129636426113 1106 60-61 0.28305840259345416 1106 62-63 0.27699348749180785 1106 64-65 0.26717401633673177 1106 66-67 0.2141298607483293 1106 68-69 0.2766459413459046 1106 70-71 0.2952081269411835 1106 72-73 0.2791320250289928 1106 74-75 0.2230884886032669 1106 76-77 0.20820191578610547 1106 78-79 0.2117769915147747 1106 80-81 0.2024111467440548 1106 82-83 0.19779750819230912 1106 84-85 0.18532486925276004 1106 86-87 0.2274360693501265 1106 88-89 0.2088655899478482 1106 90-91 0.23293018627371964 1106 92-93 0.21095555911788466 1106 94-95 0.2438482770068049 1106 96-97 0.24094765116036143 1106 98-99 0.2621251154830375 1106 100 0.18372409844523574 1107 1 0.11534746512739957 1107 2 0.14070974862940844 1107 3 0.1588114518625332 1107 4 0.17043788054209585 1107 5 0.19188791725425602 1107 6 0.18956409479509517 1107 7 0.17081025317656895 1107 8 0.2140647314341706 1107 9 0.2650376040558058 1107 10-11 0.2006130037403011 1107 12-13 0.1654880449676881 1107 14-15 0.24882249257147038 1107 16-17 0.2580779625109457 1107 18-19 0.23922391229957896 1107 20-21 0.247492736020817 1107 22-23 0.24302237370362434 1107 24-25 0.202750016524746 1107 26-27 0.20210837309066676 1107 28-29 0.2129849080828521 1107 30-31 0.22224493379938082 1107 32-33 0.2093998316724921 1107 34-35 0.18877495006822187 1107 36-37 0.21089093000528436 1107 38-39 0.21622630040341306 1107 40-41 0.1305471668339777 1107 42-43 0.1831704736411126 1107 44-45 0.16717783632763172 1107 46-47 0.16502588043361754 1107 48-49 0.1573284051193582 1107 50-51 0.14541633262278708 1107 52-53 0.10792391239024823 1107 54-55 0.10127045231890008 1107 56-57 0.1387959591864174 1107 58-59 0.1596092022686193 1107 60-61 0.2469939505384957 1107 62-63 0.24345153863660585 1107 64-65 0.2716747728545599 1107 66-67 0.24928561716124875 1107 68-69 0.2638457529741345 1107 70-71 0.3339305326911912 1107 72-73 0.23851033989426895 1107 74-75 0.2107264744240922 1107 76-77 0.21307207214866963 1107 78-79 0.22833464889728816 1107 80-81 0.21441343611387254 1107 82-83 0.18539387970433552 1107 84-85 0.16051300789251854 1107 86-87 0.06881532141160562 1107 88-89 0.11761013171253865 1107 90-91 0.14064369565384993 1107 92-93 0.1535937897287667 1107 94-95 0.09355074052726309 1107 96-97 0.07260728108443004 1107 98-99 0.0824701412326192 1107 100 0.0974741887844246 1108 1 0.05790716152950637 1108 2 0.08081337718414261 1108 3 0.07585894902408086 1108 4 0.07271030988071203 1108 5 0.04856033859410047 1108 6 0.10532212300854127 1108 7 0.09018783750252624 1108 8 0.14045923983177744 1108 9 0.14285256131791613 1108 10-11 0.1221549228682619 1108 12-13 0.1661242807448673 1108 14-15 0.1280044624472083 1108 16-17 0.1403397934746451 1108 18-19 0.13392139201935294 1108 20-21 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2214 78-79 -0.15776146900778798 2214 80-81 -0.16950394011048786 2214 82-83 -0.14109436631942174 2214 84-85 -0.11329779633162929 2214 86-87 -0.10029934813984198 2214 88-89 -0.07486369962177974 2214 90-91 -0.10340936167024495 2214 92-93 -0.13586685208466776 2214 94-95 -0.1255257089960935 2214 96-97 -0.12254383101005217 2214 98-99 -0.11014154962591505 2214 100 -0.1421278115011546 2215 1 -0.11839941744010929 2215 2 -0.19348507459093156 2215 3 -0.2362498381527658 2215 4 -0.1828953126499897 2215 5 -0.1573710480450643 2215 6 -0.18338659991724882 2215 7 -0.13958773469818198 2215 8 -0.2271579478883865 2215 9 -0.2669186711187237 2215 10-11 -0.23056334753849228 2215 12-13 -0.22238238952887457 2215 14-15 -0.3145362157959184 2215 16-17 -0.35996849885637516 2215 18-19 -0.41004464509971683 2215 20-21 -0.32002807258658095 2215 22-23 -0.33165515466693307 2215 24-25 -0.3296529588632353 2215 26-27 -0.3609868602700743 2215 28-29 -0.3461307874144737 2215 30-31 -0.3439623820659108 2215 32-33 -0.40555462147649735 2215 34-35 -0.40098381193161003 2215 36-37 -0.36446656746601747 2215 38-39 -0.3546765519822159 2215 40-41 -0.3366517808946696 2215 42-43 -0.29644456215076787 2215 44-45 -0.35055509688351094 2215 46-47 -0.25721641923116323 2215 48-49 -0.2864450342137843 2215 50-51 -0.30921497565064016 2215 52-53 -0.32490771529158735 2215 54-55 -0.2966788362464996 2215 56-57 -0.29441797948891235 2215 58-59 -0.3598566531696292 2215 60-61 -0.31121792862869313 2215 62-63 -0.297754342674736 2215 64-65 -0.2877865885948978 2215 66-67 -0.37890976889663364 2215 68-69 -0.3299518701925024 2215 70-71 -0.31532988136558515 2215 72-73 -0.3253204456144445 2215 74-75 -0.31724074653907053 2215 76-77 -0.31823969534109864 2215 78-79 -0.23475299979209296 2215 80-81 -0.3162445914132803 2215 82-83 -0.3199937462404989 2215 84-85 -0.2684011038951226 2215 86-87 -0.24977378010689222 2215 88-89 -0.2667036032466683 2215 90-91 -0.2554441289696143 2215 92-93 -0.21847207400433177 2215 94-95 -0.2863055086453805 2215 96-97 -0.31922124398390395 2215 98-99 -0.341929954820948 2215 100 -0.33140678149294445 2216 1 0.0935588781301604 2216 2 0.03662330481683895 2216 3 -0.07120855909738566 2216 4 -0.07607078587312088 2216 5 -0.06486239931135884 2216 6 -0.09750463919830565 2216 7 -0.10514992223364317 2216 8 -0.05718704237332162 2216 9 -0.2423387107798618 2216 10-11 -0.20508279358995907 2216 12-13 -0.21905165233317803 2216 14-15 -0.2828331028121056 2216 16-17 -0.2700134970190007 2216 18-19 -0.2819928409474812 2216 20-21 -0.24907308615794221 2216 22-23 -0.19308700862205086 2216 24-25 -0.25934117020378267 2216 26-27 -0.302521934042872 2216 28-29 -0.3423799288446716 2216 30-31 -0.29333442960138 2216 32-33 -0.31438851676156077 2216 34-35 -0.32626594345399695 2216 36-37 -0.416151652235051 2216 38-39 -0.3853337390110525 2216 40-41 -0.2845539289183989 2216 42-43 -0.2773910831162141 2216 44-45 -0.23677889795586538 2216 46-47 -0.18236827694040159 2216 48-49 -0.22915035438151676 2216 50-51 -0.21563763806449998 2216 52-53 -0.2774853624531346 2216 54-55 -0.2058847589889936 2216 56-57 -0.24254448796861539 2216 58-59 -0.19523094290885723 2216 60-61 -0.18363317243916555 2216 62-63 -0.18882150466205871 2216 64-65 -0.1703182476996119 2216 66-67 -0.23112159878611038 2216 68-69 -0.19000510969134154 2216 70-71 -0.23678983057281044 2216 72-73 -0.2620665559605655 2216 74-75 -0.2311200672288436 2216 76-77 -0.2997529540868342 2216 78-79 -0.18957471844454687 2216 80-81 -0.23834622086319257 2216 82-83 -0.26253505681373923 2216 84-85 -0.23500174648587802 2216 86-87 -0.24170941083526998 2216 88-89 -0.20373991135217295 2216 90-91 -0.288832396762146 2216 92-93 -0.3114895610458106 2216 94-95 -0.23273752369177103 2216 96-97 -0.1906899014585619 2216 98-99 -0.16754117947417058 2216 100 -0.1771294346148693 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 28460.0 3 4363.0 4 2719.0 5 2520.0 6 2053.0 7 2495.0 8 2862.0 9 3114.0 10 3345.0 11 3511.0 12 3839.0 13 4375.0 14 4958.0 15 5223.0 16 5270.0 17 5117.0 18 4980.0 19 4806.0 20 4740.0 21 5087.0 22 5805.0 23 8345.0 24 13443.0 25 17948.0 26 18787.0 27 15509.0 28 16812.0 29 21116.0 30 25828.0 31 31020.0 32 40378.0 33 55013.0 34 80159.0 35 126856.0 36 243611.0 37 535504.0 38 931883.0 39 457494.0 40 1029.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.365697676957176 19.711770073922278 13.373506163100574 23.54902608601997 2 15.366410647151547 19.904207365618866 40.91516424401469 23.8142177432149 3 18.313977605672957 25.450113456245475 29.702175536242688 26.533733401838877 4 12.231300665976262 16.677025165329646 39.061643740907506 32.030030427786585 5 12.871317391677717 38.14440642343246 35.60524243610699 13.379033748782835 6 30.898068108877556 38.133926791034675 17.945606341342003 13.022398758745762 7 27.657262706844175 32.47542758834497 22.25806381185676 17.60924589295409 8 25.08770263666456 36.88848765758912 20.252186243612655 17.771623462133675 9 25.03079280418105 17.339123290203396 21.040554805369467 36.58952910024609 10-11 23.861191809692368 27.19687332619175 28.744163503411656 20.197771360704223 12-13 24.43756532560236 24.504735281783606 28.87241983769686 22.18527955491717 14-15 21.745013940499312 26.521643023155328 25.846386158653345 25.88695687769202 16-17 21.557884334303505 29.96637578561716 26.322036358978966 22.15370352110037 18-19 21.464268457040063 28.892966923889723 28.27604638165459 21.366718237415622 20-21 22.664768066402253 28.241497005192773 27.683269231014133 21.410465697390844 22-23 22.330574347526692 27.99492775969079 27.789145442124518 21.885352450658 24-25 22.113783095345998 28.08143826134828 27.77863184404997 22.026146799255756 26-27 21.869639887785585 28.04536242272524 27.880824943328808 22.204172746160367 28-29 21.846565274341433 28.122125600349296 27.941656964051813 22.089652161257458 30-31 22.111061625542703 28.516495432045396 27.546760223500115 21.825682718911782 32-33 21.79744307762169 28.20413971671256 28.031724292509125 21.966692913156624 34-35 21.876701004520488 27.93167674292018 28.073942575542794 22.117679677016536 36-37 22.215757993843095 28.26303757851218 27.579418139401024 21.941786288243705 38-39 22.045471414724453 28.467589237114723 27.71169709882958 21.77524224933125 40-41 21.83804995547324 28.140855550471695 27.999516072840137 22.02157842121493 42-43 21.761469685890955 28.058696613661105 27.991562580108276 22.188271120339664 44-45 21.951066862334205 28.21753446529891 27.894522922102805 21.936875750264083 46-47 21.960151580320176 28.400150066554737 27.748412183774036 21.891286169351055 48-49 21.909320477627602 28.189286147711435 27.855411883485704 22.045981491175258 50-51 21.835441886395046 28.158571972340752 27.883955467475786 22.122030673788416 52-53 21.92892311163351 28.06083311196102 27.878228245166504 22.13201553123897 54-55 21.999285301519787 28.460829137572908 27.68796133350218 21.85192422740512 56-57 21.85693044771188 28.205568142203486 27.744261228458456 22.193240181626177 58-59 21.722610663659005 27.826740058233163 28.146034491949955 22.304614786157877 60-61 21.98493712582749 28.265458822716027 27.70933874546399 22.04026530599249 62-63 21.81921723206578 28.251821919066856 27.758177598787544 22.170783250079825 64-65 21.773870751056894 27.98429418438705 27.995156021096346 22.246679043459718 66-67 21.681767974698722 28.056429725836708 27.818543415297825 22.44325888416674 68-69 22.087419532904224 28.19666010837018 27.735972225911542 21.979948132814055 70-71 22.002409556135163 28.0037580633893 27.810142113597724 22.18369026687781 72-73 21.800715662187653 28.150107123727906 27.957491820171864 22.091685393912577 74-75 21.870398755566068 28.197726724855478 27.81062408179788 22.121250437780578 76-77 21.889142566255547 28.169940669333464 27.692508100179698 22.24840866423129 78-79 21.995709247677418 28.27204432224889 27.7380654354284 21.994180994645294 80-81 21.8534325446136 28.05548995727763 27.928352610736667 22.162724887372104 82-83 21.751170669813437 28.131498541051947 28.018479644772803 22.09885114436181 84-85 21.835433192776684 28.034765528986828 27.859798973124434 22.270002305112055 86-87 22.00552612940065 28.171979900419053 27.71188150569651 22.110612464483783 88-89 21.73856961133622 28.06783714079456 27.817499476037444 22.376093771831773 90-91 21.742561749445894 28.00396747933165 27.881893991091726 22.37157678013073 92-93 21.96311270293808 28.159806749117518 27.735533154595434 22.14154739334898 94-95 21.755922395308584 27.971531168462114 27.843872244290097 22.428674191939205 96-97 21.783867076851426 27.90070787798519 27.930889568487334 22.38453547667605 98-99 21.831915916661103 28.047075928864707 27.766709595165278 22.354298559308912 100 21.982319805151096 27.960046369032586 27.64946408546689 22.408169740349425 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2440.0 1 1463.5 2 450.0 3 367.5 4 283.0 5 223.5 6 193.0 7 193.0 8 197.0 9 201.5 10 209.0 11 195.0 12 205.5 13 244.5 14 293.0 15 358.5 16 452.0 17 590.5 18 834.5 19 1193.0 20 1685.5 21 2468.0 22 3778.0 23 5843.5 24 8572.5 25 11975.5 26 16544.0 27 22274.0 28 29523.0 29 37970.0 30 45918.0 31 53878.0 32 61490.0 33 68757.5 34 76214.0 35 82012.5 36 86723.5 37 91553.5 38 95414.0 39 96726.0 40 97958.5 41 99837.0 42 99601.5 43 98041.0 44 95682.5 45 93630.5 46 92682.5 47 89996.0 48 87643.0 49 86662.5 50 84036.5 51 80853.0 52 76445.0 53 72500.5 54 70083.0 55 66560.0 56 61727.5 57 56962.5 58 52190.5 59 47284.5 60 41946.0 61 35862.5 62 30741.5 63 25860.5 64 21077.0 65 17282.5 66 13802.5 67 10636.0 68 8204.5 69 6363.0 70 4813.0 71 3571.5 72 2787.0 73 2184.5 74 1695.5 75 1261.5 76 885.0 77 650.0 78 479.0 79 341.0 80 244.5 81 156.0 82 105.5 83 79.0 84 58.0 85 43.5 86 39.5 87 35.5 88 24.0 89 15.5 90 13.0 91 11.5 92 11.5 93 8.5 94 5.5 95 4.5 96 1.5 97 2.0 98 2.5 99 1.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07580778925943607 2 0.07813474298250749 3 0.0792982198440432 4 0.08111615244019274 5 0.07958908905942712 6 0.07958908905942712 7 0.07900735062865927 8 0.0788982746728903 9 0.0788982746728903 10-11 0.07828017759019945 12-13 0.07724395601039422 14-15 0.07597140319308954 16-17 0.0748988229613613 18-19 0.07449887779020839 20-21 0.074044394641171 22-23 0.07578960993347457 24-25 0.07586232723732056 26-27 0.07515333352482224 28-29 0.07188105485175306 30-31 0.07638952769020393 32-33 0.07537148543636019 34-35 0.07275366249790483 36-37 0.06973589438829658 38-39 0.07159018563636912 40-41 0.07378988407771007 42-43 0.07793477039693103 44-45 0.07918914388827424 46-47 0.08227962930172845 48-49 0.08495199021806829 50-51 0.08591549449402755 52-53 0.0860609291017195 54-55 0.08598821179787353 56-57 0.08417027920172399 58-59 0.08313405762191875 60-61 0.08106161446230827 62-63 0.08160699424115311 64-65 0.08082528322480881 66-67 0.08055259333538639 68-69 0.080134468838272 70-71 0.07828017759019945 72-73 0.07828017759019945 74-75 0.07778933578923908 76-77 0.07764390118154711 78-79 0.07784387376712357 80-81 0.07882555736904433 82-83 0.07868012276135236 84-85 0.07771661848539309 86-87 0.07855286747962188 88-89 0.07595322386712804 90-91 0.07389896003347905 92-93 0.07389896003347905 94-95 0.0740989326190555 96-97 0.07351719418828764 98-99 0.07302635238732727 100 0.07239007597867492 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2750377.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.82857940219603 #Duplication Level Percentage of deduplicated Percentage of total 1 78.81038482938226 53.455964451173784 2 13.465041331368072 18.26629250197101 3 3.748950767273687 7.628580143788409 4 1.5114664689431674 4.100824936098739 5 0.7292527604634327 2.473208938368229 6 0.43408886512124356 1.7666178633291256 7 0.2894831521331092 1.3744661679041013 8 0.19217893779983633 1.0428179473588712 9 0.14155800116293507 0.864151030970667 >10 0.651208460673909 7.536690117565538 >50 0.022221260605000046 1.0054138890301383 >100 0.004004209219492766 0.3697205868075586 >500 1.0730390256083659E-4 0.04825493855283719 >1k 5.365195127606727E-5 0.06699648708106337 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 3.635865192299092E-5 0.0 0.0 3.635865192299092E-5 0.0 8 3.635865192299092E-5 0.0 0.0 3.635865192299092E-5 0.0 9 3.635865192299092E-5 0.0 0.0 3.635865192299092E-5 0.0 10-11 3.635865192299092E-5 0.0 0.0 3.635865192299092E-5 0.0 12-13 3.635865192299092E-5 0.0 0.0 3.635865192299092E-5 0.0 14-15 3.635865192299092E-5 0.0 0.0 3.635865192299092E-5 0.0 16-17 3.635865192299092E-5 0.0 0.0 3.635865192299092E-5 0.0 18-19 3.635865192299092E-5 0.0 0.0 3.635865192299092E-5 0.0 20-21 3.635865192299092E-5 0.0 0.0 3.635865192299092E-5 0.0 22-23 3.635865192299092E-5 0.0 0.0 1.0907595576897276E-4 0.0 24-25 3.635865192299092E-5 3.635865192299092E-5 0.0 1.8179325961495461E-4 0.0 26-27 5.453797788448638E-5 3.635865192299092E-5 0.0 4.181244971143956E-4 0.0 28-29 1.0907595576897276E-4 3.635865192299092E-5 0.0 0.0011271182096127185 0.0 30-31 1.4543460769196368E-4 3.635865192299092E-5 0.0 0.004308500252874424 0.0 32-33 1.8179325961495461E-4 3.635865192299092E-5 0.0 0.011707485919203077 0.0 34-35 1.8179325961495461E-4 3.635865192299092E-5 0.0 0.023287716556675685 0.0 36-37 1.9997258557645007E-4 3.635865192299092E-5 0.0 0.044230300064318454 0.0 38-39 2.1815191153794553E-4 3.635865192299092E-5 0.0 0.08282500908057332 0.0 40-41 2.1815191153794553E-4 3.635865192299092E-5 0.0 0.13087296759680583 0.0 42-43 2.1815191153794553E-4 3.635865192299092E-5 0.0 0.17922997465438376 0.0 44-45 2.1815191153794553E-4 3.635865192299092E-5 0.0 0.23411335973213854 0.0 46-47 2.1815191153794553E-4 3.635865192299092E-5 0.0 0.30175863163486316 0.0 48-49 2.1815191153794553E-4 3.635865192299092E-5 0.0 0.3791843809048723 0.0 50-51 2.1815191153794553E-4 3.635865192299092E-5 0.0 0.46586340708928264 0.0 52-53 2.1815191153794553E-4 3.635865192299092E-5 0.0 0.5530514544006149 0.0 54-55 2.1815191153794553E-4 3.635865192299092E-5 0.0 0.6473476181628919 0.0 56-57 2.1815191153794553E-4 7.271730384598184E-5 0.0 0.7567871604510945 0.0 58-59 2.1815191153794553E-4 7.271730384598184E-5 0.0 0.8857876574738663 0.0 60-61 2.1815191153794553E-4 7.271730384598184E-5 0.0 1.024986756361037 0.0 62-63 2.1815191153794553E-4 7.271730384598184E-5 0.0 1.1695305770808875 0.0 64-65 2.1815191153794553E-4 7.271730384598184E-5 0.0 1.3219460459420653 0.0 66-67 2.1815191153794553E-4 7.271730384598184E-5 0.0 1.4970311342772282 0.0 68-69 2.1815191153794553E-4 7.271730384598184E-5 0.0 1.6923134537556124 0.0 70-71 2.1815191153794553E-4 7.271730384598184E-5 0.0 1.9041389598589575 0.0 72-73 2.1815191153794553E-4 7.271730384598184E-5 0.0 2.13076243729496 0.0 74-75 2.1815191153794553E-4 7.271730384598184E-5 0.0 2.353386463019433 0.0 76-77 2.1815191153794553E-4 7.271730384598184E-5 0.0 2.5922809854794453 0.0 78-79 2.1815191153794553E-4 7.271730384598184E-5 0.0 2.8677523117739856 0.0 80-81 2.1815191153794553E-4 7.271730384598184E-5 0.0 3.171674283198267 0.0 82-83 2.1815191153794553E-4 7.271730384598184E-5 0.0 3.4887944452705937 0.0 84-85 2.1815191153794553E-4 7.271730384598184E-5 0.0 3.795770543456406 0.0 86-87 2.1815191153794553E-4 7.271730384598184E-5 0.0 4.115672142400841 0.0 88 2.1815191153794553E-4 7.271730384598184E-5 0.0 4.378417940522335 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 3965 0.0 36.984116 1 TCAACGC 4020 0.0 35.542126 4 ATCAACG 4050 0.0 35.395546 3 CAACGCA 4090 0.0 35.048744 5 AACGCAG 4270 0.0 33.35114 6 TATCAAC 4630 0.0 30.96155 2 GGTATCA 2395 0.0 30.810446 1 GTACATG 9205 0.0 29.818987 1 TACATGG 9200 0.0 28.76235 2 ACATGGG 9400 0.0 27.050371 3 ACGCAGA 5240 0.0 26.90779 7 CGCAGAG 5460 0.0 25.478813 8 TACACCG 375 0.0 25.066555 5 CATGGGG 5965 0.0 23.716576 4 ACACCGT 530 0.0 23.056501 6 ACCGTTC 340 0.0 22.116743 8 GCAGAGT 6505 0.0 20.662508 9 GAGTACA 6500 0.0 20.246433 1 TAGCGCG 120 0.0050660213 19.583246 4 GTACACG 325 5.620677E-10 18.800259 1 >>END_MODULE