FastQCFastQC Report
Wed 25 May 2016
SRR1032930_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032930_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2750377
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55160.20055432400721795No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45720.16623175659191447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41050.048.985471
GTATCAA77150.041.532221
TCAACGC89350.035.1712344
ATCAACG90450.034.74353
CAACGCA90450.034.7384385
AACGCAG94250.033.4869586
TATCAAC94600.033.4188652
ACGCAGA110200.028.5537557
CGCAGAG112050.028.0812978
GTACATG85700.026.3146211
TACATGG88050.024.8983232
ACCGTCG1151.371337E-424.5389678
GCAGAGT126700.024.4672729
ACATGGG86200.024.231563
GTGGTAT23000.023.0827371
TGGTATC22300.021.5183452
ACCGTTC3301.8189894E-1221.3786478
GAGTACT81450.020.84661112-13
CAGAGTA124200.020.04345110-11
AGAGTAC116500.020.03773710-11
Produced by FastQC (version 0.11.5)