##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1032930_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2750377
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.889843828682395	34.0	31.0	34.0	31.0	34.0
2	32.94180252379947	34.0	31.0	34.0	31.0	34.0
3	33.014024622806254	34.0	33.0	34.0	31.0	34.0
4	36.4056305735541	37.0	37.0	37.0	35.0	37.0
5	36.38910847494725	37.0	37.0	37.0	35.0	37.0
6	36.44684819572008	37.0	37.0	37.0	35.0	37.0
7	36.412377648591445	37.0	37.0	37.0	35.0	37.0
8	36.35563124618916	37.0	37.0	37.0	35.0	37.0
9	38.208094017656485	39.0	39.0	39.0	37.0	39.0
10-11	38.21848895624127	39.0	39.0	39.0	37.0	39.0
12-13	38.21318259278637	39.0	39.0	39.0	37.0	39.0
14-15	39.785991338641935	41.0	40.0	41.0	38.0	41.0
16-17	39.79922825125428	41.0	40.0	41.0	38.0	41.0
18-19	39.820791295156994	41.0	40.0	41.0	37.5	41.0
20-21	39.833155600123185	41.0	40.0	41.0	38.0	41.0
22-23	39.794046052595704	41.0	40.0	41.0	37.0	41.0
24-25	39.77301057273239	41.0	40.0	41.0	37.0	41.0
26-27	39.69707171053277	41.0	40.0	41.0	37.0	41.0
28-29	39.638096522767604	41.0	40.0	41.0	37.0	41.0
30-31	39.52796125767486	41.0	40.0	41.0	36.5	41.0
32-33	39.400608898343755	41.0	40.0	41.0	36.0	41.0
34-35	39.306815211151054	41.0	39.0	41.0	35.0	41.0
36-37	39.21941664724508	41.0	39.0	41.0	35.0	41.0
38-39	39.11961996482664	41.0	39.0	41.0	35.0	41.0
40-41	39.01338761922457	41.0	39.0	41.0	35.0	41.0
42-43	38.87640912500359	41.0	39.0	41.0	35.0	41.0
44-45	38.705444744484126	41.0	39.0	41.0	35.0	41.0
46-47	38.4828538051329	41.0	39.0	41.0	35.0	41.0
48-49	38.280699155061285	41.0	39.0	41.0	35.0	41.0
50-51	38.13043829991307	41.0	38.0	41.0	34.0	41.0
52-53	37.994191705355306	40.5	38.0	41.0	34.0	41.0
54-55	37.850267981444	40.0	38.0	41.0	34.0	41.0
56-57	37.64650827868325	40.0	37.0	41.0	33.5	41.0
58-59	37.48498860338056	40.0	37.0	41.0	33.0	41.0
60-61	37.30171900070427	40.0	36.0	41.0	33.0	41.0
62-63	37.07019383160927	39.5	36.0	41.0	33.0	41.0
64-65	36.787318974816905	39.0	35.0	41.0	32.5	41.0
66-67	36.51911537945526	39.0	35.0	41.0	32.0	41.0
68-69	36.22658493726496	38.0	35.0	40.5	32.0	41.0
70-71	35.90244428309283	37.0	35.0	40.0	32.0	41.0
72-73	35.56479838945715	37.0	35.0	39.5	31.0	41.0
74-75	35.217631801022186	36.0	35.0	39.0	31.0	41.0
76-77	34.862885888007355	36.0	35.0	39.0	31.0	40.5
78-79	34.515053572655674	35.5	35.0	37.5	31.0	39.5
80-81	34.17938322637224	35.0	35.0	37.0	30.5	39.0
82-83	33.86691060898197	35.0	34.0	37.0	30.0	39.0
84-85	33.58299025915356	35.0	34.0	36.0	30.0	37.5
86-87	33.42824856374235	35.0	34.0	36.0	30.0	37.0
88-89	33.23473418371373	35.0	34.0	36.0	30.0	37.0
90-91	33.058533066557786	35.0	34.0	35.0	30.0	36.5
92-93	32.89630057988414	35.0	34.0	35.0	30.0	36.0
94-95	32.79636009899734	35.0	34.0	35.0	30.0	36.0
96-97	32.73534410009973	35.0	34.0	35.0	30.0	36.0
98-99	32.630498837068515	35.0	34.0	35.0	30.0	36.0
100	32.549807899062564	35.0	34.0	35.0	30.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.12828969748514396
1101	2	0.15077372179327853
1101	3	0.10355358644423518
1101	4	0.08103859381441225
1101	5	0.08660241655175582
1101	6	0.08591187531028766
1101	7	0.0606794091935825
1101	8	-0.008418465372926676
1101	9	0.06277572393375408
1101	10-11	0.09386902207159409
1101	12-13	0.13433208838665678
1101	14-15	0.18515787843607967
1101	16-17	0.16867317595664133
1101	18-19	0.13086882183223025
1101	20-21	0.12767641538227537
1101	22-23	0.1468773348876553
1101	24-25	0.1922647411608196
1101	26-27	0.20840748750548244
1101	28-29	0.2215524425754154
1101	30-31	0.1433007721719619
1101	32-33	0.1321321031870255
1101	34-35	0.14843598807559744
1101	36-37	0.141348452420047
1101	38-39	0.16233501974240738
1101	40-41	0.1907996370259326
1101	42-43	0.1327866672082152
1101	44-45	0.10514783453621135
1101	46-47	0.13038729748303268
1101	48-49	0.049791947457755725
1101	50-51	0.08364960138470678
1101	52-53	0.09250470470147576
1101	54-55	0.054290897648897385
1101	56-57	0.08850673640044704
1101	58-59	0.07179591656982609
1101	60-61	0.11646623142686963
1101	62-63	0.0880368295630305
1101	64-65	0.07849128026226282
1101	66-67	0.1320372834146184
1101	68-69	0.12956840678458548
1101	70-71	0.19322696392863747
1101	72-73	0.1711992929850794
1101	74-75	0.1936040830151171
1101	76-77	0.16353518039205284
1101	78-79	0.057171528748156675
1101	80-81	0.10004201614655273
1101	82-83	0.10064621494715453
1101	84-85	0.09685214951684173
1101	86-87	0.04470562884402085
1101	88-89	0.0716317747824533
1101	90-91	0.13554523545234076
1101	92-93	0.16228164274930634
1101	94-95	0.14538740623839175
1101	96-97	0.14034788272773113
1101	98-99	0.19297058883085327
1101	100	0.12622811848995497
1102	1	0.17753668924802923
1102	2	0.2009660135963216
1102	3	0.20590369594415847
1102	4	0.12926886591630904
1102	5	0.1304609236880765
1102	6	0.12756254814866708
1102	7	0.12012545818699039
1102	8	0.1203803800835459
1102	9	0.1574981112180467
1102	10-11	0.15902341955371924
1102	12-13	0.14382472400999546
1102	14-15	0.23443814091881165
1102	16-17	0.21372176873857995
1102	18-19	0.21165302603777292
1102	20-21	0.2069577205762272
1102	22-23	0.2595342027112082
1102	24-25	0.26597485859998216
1102	26-27	0.2479406486187017
1102	28-29	0.22782170567431592
1102	30-31	0.25139585462998326
1102	32-33	0.27314217937506413
1102	34-35	0.2778451016155614
1102	36-37	0.261788153375349
1102	38-39	0.2650663895875027
1102	40-41	0.2328766933850872
1102	42-43	0.24140249550355009
1102	44-45	0.31353409105354046
1102	46-47	0.3259160404885577
1102	48-49	0.290542458675894
1102	50-51	0.2484784260082975
1102	52-53	0.2984980990344326
1102	54-55	0.297561827866204
1102	56-57	0.297778268463361
1102	58-59	0.32452430615112604
1102	60-61	0.26662667023146014
1102	62-63	0.30816776451828076
1102	64-65	0.33740341232925886
1102	66-67	0.31046388553887994
1102	68-69	0.3477472689549259
1102	70-71	0.3517517303018707
1102	72-73	0.38815712354816867
1102	74-75	0.3901694776249087
1102	76-77	0.3808373419446056
1102	78-79	0.4053354728307852
1102	80-81	0.36799902016282005
1102	82-83	0.39574781983827023
1102	84-85	0.3869128782988085
1102	86-87	0.3578049400753258
1102	88-89	0.3388506871248964
1102	90-91	0.3338271019420205
1102	92-93	0.3845869888394873
1102	94-95	0.3509237759752537
1102	96-97	0.37451326861778256
1102	98-99	0.45364199119512705
1102	100	0.4454006509153743
1103	1	0.10715062386545071
1103	2	0.12820862039655623
1103	3	0.14574866769505235
1103	4	0.03645730705050454
1103	5	0.06443459692226128
1103	6	0.042752030311575595
1103	7	0.0814402108047858
1103	8	-0.025003703207936212
1103	9	0.02291470080339053
1103	10-11	0.0819638832826044
1103	12-13	0.1302426602682445
1103	14-15	0.16294626979035343
1103	16-17	0.16096338736587512
1103	18-19	0.1530671532340122
1103	20-21	0.19156592149246876
1103	22-23	0.17644250255964522
1103	24-25	0.1628380414590893
1103	26-27	0.14309162076256143
1103	28-29	0.13344472573767518
1103	30-31	0.15513301556314474
1103	32-33	0.17040795653287688
1103	34-35	0.18171626914190142
1103	36-37	0.18110325759887047
1103	38-39	0.14657885148251637
1103	40-41	0.12592650534497096
1103	42-43	0.14372016323154924
1103	44-45	0.15779343819294667
1103	46-47	0.21457652609512223
1103	48-49	0.17328510971410793
1103	50-51	0.1700265425621481
1103	52-53	0.1548023839132
1103	54-55	0.13176206272963498
1103	56-57	0.17610947416074652
1103	58-59	0.13376355086526104
1103	60-61	0.16222993258103457
1103	62-63	0.12673648218002853
1103	64-65	0.1962750796872612
1103	66-67	0.1656943941147233
1103	68-69	0.1168254702043896
1103	70-71	0.143588933404736
1103	72-73	0.19690170726882172
1103	74-75	0.1410828194534659
1103	76-77	0.17763479981223895
1103	78-79	0.1679944279183303
1103	80-81	0.1639530839091634
1103	82-83	0.18662672498859934
1103	84-85	0.11891678388003157
1103	86-87	0.18859267478077157
1103	88-89	0.1324928857867249
1103	90-91	0.13614138000698972
1103	92-93	0.15358863541138845
1103	94-95	0.16158050307750926
1103	96-97	0.1970158625717673
1103	98-99	0.11411840546020358
1103	100	0.07451521348223622
1104	1	0.09103192587422626
1104	2	0.11779579384369754
1104	3	0.10615271943223803
1104	4	0.08833500893985757
1104	5	0.06373729790833238
1104	6	0.06557402190133388
1104	7	0.06711478087277811
1104	8	0.01485039578556524
1104	9	0.08475514640102944
1104	10-11	0.12319492200616367
1104	12-13	0.13941393869369278
1104	14-15	0.14822203399394596
1104	16-17	0.12602770160315657
1104	18-19	0.13981945675896412
1104	20-21	0.1582223010687187
1104	22-23	0.1909308982054867
1104	24-25	0.1438485457012959
1104	26-27	0.14723184639213827
1104	28-29	0.19936145355185886
1104	30-31	0.1817904233956611
1104	32-33	0.24820861689887863
1104	34-35	0.22305234077916936
1104	36-37	0.19546162704794767
1104	38-39	0.18595240706771676
1104	40-41	0.23882632656148672
1104	42-43	0.27875832824643965
1104	44-45	0.3107213265946811
1104	46-47	0.2685818151628254
1104	48-49	0.2706615213252306
1104	50-51	0.2917555126840341
1104	52-53	0.31529427209869
1104	54-55	0.2761926423917913
1104	56-57	0.24790512473182957
1104	58-59	0.27433878078308993
1104	60-61	0.23937472301643226
1104	62-63	0.25361155569967764
1104	64-65	0.2716729550952053
1104	66-67	0.34934320425786325
1104	68-69	0.38662249350437605
1104	70-71	0.3050131457563623
1104	72-73	0.37671482950308643
1104	74-75	0.35079028530027756
1104	76-77	0.3829912950367529
1104	78-79	0.3199177289659474
1104	80-81	0.3050548027678701
1104	82-83	0.31194443851612874
1104	84-85	0.28526406174476193
1104	86-87	0.28609395093236856
1104	88-89	0.25578950525501654
1104	90-91	0.28444116438559064
1104	92-93	0.33729429169017777
1104	94-95	0.36638392003963105
1104	96-97	0.33677989745478243
1104	98-99	0.37773342848798563
1104	100	0.4192308591959275
1105	1	0.10030033405668348
1105	2	0.1376268761266033
1105	3	0.1292547506106203
1105	4	0.11125923403012195
1105	5	0.10697188903552757
1105	6	0.09270965411250387
1105	7	0.10539387314530302
1105	8	0.014780987609810836
1105	9	0.025999804484882816
1105	10-11	0.11782745308323683
1105	12-13	0.10999850247007714
1105	14-15	0.19707246833316105
1105	16-17	0.21346186372725384
1105	18-19	0.2154602869142579
1105	20-21	0.1900799770587156
1105	22-23	0.20418370301758415
1105	24-25	0.17328144674907975
1105	26-27	0.13259584039506223
1105	28-29	0.16406920066430075
1105	30-31	0.1878460128354078
1105	32-33	0.14681880357013455
1105	34-35	0.17779012223351032
1105	36-37	0.13303854316472297
1105	38-39	0.15056835107304067
1105	40-41	0.16444467156123466
1105	42-43	0.10666307651323592
1105	44-45	0.10821540144363695
1105	46-47	0.16262005242973743
1105	48-49	0.20628021819929643
1105	50-51	0.2169834447508947
1105	52-53	0.21748133209467824
1105	54-55	0.239692416573547
1105	56-57	0.2416514609855085
1105	58-59	0.23360552626272835
1105	60-61	0.27718410174301056
1105	62-63	0.24675767556998096
1105	64-65	0.20860173934237025
1105	66-67	0.23984890694185879
1105	68-69	0.2732114999195687
1105	70-71	0.3223479826542359
1105	72-73	0.2852677212397339
1105	74-75	0.30592795322682065
1105	76-77	0.3006612116644547
1105	78-79	0.31564016818450114
1105	80-81	0.29016339788452683
1105	82-83	0.337386401680952
1105	84-85	0.3484800550898228
1105	86-87	0.2838524921980792
1105	88-89	0.28073459094995457
1105	90-91	0.2596801682487282
1105	92-93	0.2739038434409693
1105	94-95	0.2603600315113539
1105	96-97	0.28641337459687577
1105	98-99	0.36157292378936745
1105	100	0.40918349407721877
1106	1	0.036018815354253775
1106	2	0.07191885910734186
1106	3	0.02223677377023847
1106	4	0.018759209350221795
1106	5	0.034486771016240425
1106	6	0.04560287898589621
1106	7	0.034025767475839075
1106	8	0.013196784721273502
1106	9	0.05126141144264551
1106	10-11	0.06251967002944525
1106	12-13	0.038431600192609494
1106	14-15	0.05862994288818868
1106	16-17	0.07498602041501812
1106	18-19	0.048329350163911045
1106	20-21	0.063386318806252
1106	22-23	0.11006085140302702
1106	24-25	0.07250637443696917
1106	26-27	0.11032506732182412
1106	28-29	0.12178722291569954
1106	30-31	0.1586109417079271
1106	32-33	0.17577153662245593
1106	34-35	0.14442622695527518
1106	36-37	0.13229395053117798
1106	38-39	0.17775326276834846
1106	40-41	0.24214638039763514
1106	42-43	0.15667356547145062
1106	44-45	0.1960395719521344
1106	46-47	0.20212652843594014
1106	48-49	0.22417721909764765
1106	50-51	0.2561766094053297
1106	52-53	0.2359483153222186
1106	54-55	0.2844658303962646
1106	56-57	0.33288898529521305
1106	58-59	0.2411343770677874
1106	60-61	0.2921222292295127
1106	62-63	0.2864654161743445
1106	64-65	0.3109164673204958
1106	66-67	0.2617206060050137
1106	68-69	0.2474635754993102
1106	70-71	0.24313117465400325
1106	72-73	0.2731355751742086
1106	74-75	0.3959060621540331
1106	76-77	0.32209175748516117
1106	78-79	0.2656222752557795
1106	80-81	0.2246941589139766
1106	82-83	0.2127211845471706
1106	84-85	0.2535363499455414
1106	86-87	0.27168863245888986
1106	88-89	0.2402616499941459
1106	90-91	0.27897121339320563
1106	92-93	0.27835819549482466
1106	94-95	0.2158753725382354
1106	96-97	0.2884772313744648
1106	98-99	0.2315530317886214
1106	100	0.25916078856767655
1107	1	0.06410975602414482
1107	2	0.05270703644724506
1107	3	0.045225357228495966
1107	4	0.016528006312022114
1107	5	0.05799022954997213
1107	6	0.06639732761378525
1107	7	0.060425208888254645
1107	8	0.05204659786486587
1107	9	0.005752376358401534
1107	10-11	0.03618090157985421
1107	12-13	0.05234622441656711
1107	14-15	0.0589362218695797
1107	16-17	0.101916423106303
1107	18-19	0.10821426386959132
1107	20-21	0.11088520559435722
1107	22-23	0.08747803659719011
1107	24-25	0.06509838635474807
1107	26-27	0.07406070339128945
1107	28-29	0.10376822720768075
1107	30-31	0.14187190655375304
1107	32-33	0.1211209336229544
1107	34-35	0.16102404156294625
1107	36-37	0.198441118310285
1107	38-39	0.22017858431756565
1107	40-41	0.15750141922600136
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2216	10-11	-0.16421558086486243
2216	12-13	-0.15595965850376103
2216	14-15	-0.2454200137137761
2216	16-17	-0.26319151108928196
2216	18-19	-0.22406983322489538
2216	20-21	-0.2291511826547179
2216	22-23	-0.243269205522175
2216	24-25	-0.24372501853465423
2216	26-27	-0.22545675838475887
2216	28-29	-0.20464620564423797
2216	30-31	-0.2324114604932035
2216	32-33	-0.2216824930721586
2216	34-35	-0.2675190589448633
2216	36-37	-0.23203027592035141
2216	38-39	-0.22253735080134618
2216	40-41	-0.14154098703716755
2216	42-43	-0.16847885456790834
2216	44-45	-0.2196524111944811
2216	46-47	-0.18400137985528886
2216	48-49	-0.2839499017060163
2216	50-51	-0.22831740588074467
2216	52-53	-0.18753289706376108
2216	54-55	-0.19158841619272948
2216	56-57	-0.26795512090639306
2216	58-59	-0.34583768532575476
2216	60-61	-0.3521340931843753
2216	62-63	-0.3348155252852436
2216	64-65	-0.3647604654250003
2216	66-67	-0.3666873374644979
2216	68-69	-0.35788037071395706
2216	70-71	-0.3238336259928474
2216	72-73	-0.29334379974076796
2216	74-75	-0.35228242903127693
2216	76-77	-0.3206545431434833
2216	78-79	-0.2692877097417181
2216	80-81	-0.2820529426144418
2216	82-83	-0.29244663077836464
2216	84-85	-0.2922959056219554
2216	86-87	-0.2190219361607859
2216	88-89	-0.25841863771663753
2216	90-91	-0.3244456875079038
2216	92-93	-0.286094069537846
2216	94-95	-0.32344483025418214
2216	96-97	-0.28892024674927796
2216	98-99	-0.2886572331405972
2216	100	-0.3387230541417381
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	20.0
3	6.0
4	8.0
5	12.0
6	50.0
7	168.0
8	283.0
9	587.0
10	1120.0
11	1827.0
12	2907.0
13	4640.0
14	5306.0
15	7088.0
16	8943.0
17	12625.0
18	15605.0
19	8536.0
20	5703.0
21	5223.0
22	5294.0
23	5721.0
24	6178.0
25	7312.0
26	9086.0
27	11515.0
28	14912.0
29	19189.0
30	24076.0
31	30806.0
32	39385.0
33	51852.0
34	72369.0
35	113135.0
36	216253.0
37	508078.0
38	984082.0
39	548260.0
40	2217.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.36024974121636	19.682355118426013	13.557499780938326	23.399895359419308
2	15.546300218334657	20.054720009889596	40.56465232796374	23.834327443812004
3	18.366992998043173	25.466645627103805	30.02431669292976	26.14204468192326
4	12.233864991232773	16.793478142293093	38.839984087353734	32.1326727791204
5	12.514234479508893	37.88571258204369	35.61481316654814	13.985239771899282
6	30.69119119100921	38.178171612282526	17.628344158287828	13.502293038420438
7	27.161984543813432	32.71774007355414	22.345588114988793	17.774687267643632
8	25.329413850691452	36.11881653857594	20.16998340870473	18.38178620202787
9	25.226163358281813	16.405056111346436	21.258573955536324	37.11020657483543
10-11	23.97253176564522	26.69237708139648	28.67979553348505	20.655295619473257
12-13	24.95880560243807	23.964304490952866	29.137491846563414	21.93939806004565
14-15	21.976760610877033	25.846635011572065	26.64469025752321	25.5319141200277
16-17	21.113796399548136	29.483394458286995	27.096194448979176	22.306614693185697
18-19	20.90391012419043	27.89527093234263	29.97387990647087	21.22693903699607
20-21	22.04586862091997	27.182019046843397	29.730033373606602	21.042078958630032
22-23	21.6676547150085	27.027367030235734	29.811954494147425	21.49302376060834
24-25	21.39983754865723	27.095469614545287	29.869740783213267	21.634952053584218
26-27	21.241681233881753	27.06438572712902	29.929086524257936	21.764846514731296
28-29	21.361398298197585	27.12826205764573	29.932261704209562	21.578077939947125
30-31	21.534877412138275	27.550722814193442	29.50361570736298	21.410784066305304
32-33	21.22387937578697	27.294055611871503	29.95073390033138	21.531331112010147
34-35	21.335787449132468	27.004584010460903	29.9279021655899	21.73172637481673
36-37	21.65057269421064	27.44174762754999	29.378789273045165	21.528890405194204
38-39	21.562198211502473	27.587674389421057	29.419529432733103	21.430597966343363
40-41	21.41553390959297	27.306125336925994	29.572603494190204	21.705737259290828
42-43	21.403925208448133	27.314150386824938	29.3994958277628	21.882428576964127
44-45	21.610197561186894	27.494738665837765	29.18821126285851	21.706852510116832
46-47	21.67473330762249	27.73802748560863	28.873866831536393	21.713372375232485
48-49	21.73153477831433	27.65315651356187	28.67813413232982	21.93717457579398
50-51	21.707467156708134	27.68910224968564	28.568363168921074	22.035067424685153
52-53	21.75925454100738	27.696760000698838	28.487687303815086	22.056298154478693
54-55	21.8218668945837	28.04921085971764	28.29162144635708	21.837300799341584
56-57	21.633950045866293	27.735231481473054	28.453042142579115	22.177776330081535
58-59	21.526985882443277	27.390119802808737	28.801857279247482	22.281037035500507
60-61	21.76596580542453	27.725970296235197	28.41675086936672	22.091313028973552
62-63	21.555394380154222	27.738949709756906	28.467659848413447	22.23799606167543
64-65	21.610273791556576	27.449546007692767	28.677614014369667	22.262566186380994
66-67	21.485658418038405	27.555663742763947	28.507860651078133	22.45081718811952
68-69	21.90734389080469	27.65888790781037	28.393696931090656	22.04007127029428
70-71	21.82586241813395	27.45063676725045	28.46455231410094	22.258948500514656
72-73	21.668447634633363	27.64591908672884	28.505982997967187	22.17965028067061
74-75	21.711187229968836	27.683895698662404	28.307646551727274	22.297270519641486
76-77	21.750979411898378	27.700004544834794	28.139544607553514	22.409471435713314
78-79	21.79914277190777	27.74265632359788	28.270111382932484	22.188089521561867
80-81	21.68229939762705	27.598370766141677	28.356704410039	22.36262542619227
82-83	21.553094798414616	27.61216387740863	28.472356294946177	22.36238502923058
84-85	21.56584351890668	27.528080695846423	28.2777960984985	22.6282796867484
86-87	21.671343322925615	27.634339109844614	28.230715042108084	22.46360252512169
88-89	21.37072466613583	27.525751080763676	28.326443351258195	22.777080901842297
90-91	21.359052273828365	27.44135567091367	28.353323049877787	22.84626900538018
92-93	21.520259675805193	27.58470050657143	28.210183967327822	22.684855850295556
94-95	21.24262913065009	27.341989431178675	28.35704058459384	23.058340853577395
96-97	21.23264075416721	27.315837151453902	28.367204212230966	23.08431788214792
98-99	21.225097950243892	27.408216185714245	28.274715893390578	23.091969970651284
100	21.386704440882102	27.285059466393154	28.13559741082768	23.192638681897062
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	51.0
1	49.5
2	176.0
3	300.0
4	188.5
5	125.5
6	348.0
7	746.0
8	1283.5
9	1573.0
10	1297.0
11	1006.5
12	1062.5
13	1240.0
14	1429.5
15	1619.5
16	1858.5
17	2193.5
18	2486.0
19	2822.5
20	3390.5
21	4210.5
22	5468.5
23	7276.0
24	9779.5
25	13177.0
26	17525.5
27	22884.0
28	29726.0
29	37908.5
30	45629.0
31	53414.5
32	61237.0
33	68425.0
34	75671.5
35	81142.0
36	85468.0
37	89876.0
38	93750.0
39	95807.0
40	97176.5
41	98490.0
42	98307.0
43	97305.5
44	95216.0
45	93494.0
46	92443.5
47	89930.0
48	87702.5
49	86400.0
50	83774.0
51	80236.5
52	75401.0
53	71818.5
54	69525.5
55	65847.5
56	61500.0
57	56887.0
58	51966.0
59	47255.0
60	42272.0
61	35894.5
62	30271.5
63	25595.5
64	20759.0
65	16701.5
66	13368.5
67	10319.5
68	7964.0
69	6128.5
70	4516.0
71	3280.0
72	2473.0
73	1868.5
74	1435.0
75	1055.0
76	719.0
77	498.5
78	382.0
79	273.5
80	154.5
81	78.0
82	41.5
83	23.0
84	13.5
85	6.5
86	2.0
87	3.0
88	2.5
89	1.0
90	0.5
91	0.0
92	1.0
93	1.0
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.6358651922990923E-4
2	4.3630382307589105E-4
3	1.0907595576897276E-4
4	0.013198190648045702
5	4.72662474998882E-4
6	0.0034904305846071284
7	7.998903423058002E-4
8	0.1365630966227539
9	0.0015270633807656186
10-11	0.0
12-13	1.0907595576897276E-4
14-15	1.2725528173046822E-4
16-17	0.0
18-19	1.4543460769196368E-4
20-21	0.0
22-23	1.9997258557645004E-4
24-25	5.453797788448638E-4
26-27	0.007071757799021734
28-29	0.008507924549979876
30-31	0.015670578978809087
32-33	0.018706526414378827
34-35	0.025832822191285052
36-37	0.036504086530682885
38-39	0.04152158049605563
40-41	0.05244735539891441
42-43	0.06949956315079715
44-45	0.10816698947089798
46-47	0.11480244344684383
48-49	0.11262092433146438
50-51	0.1136753252372311
52-53	0.10794883755936005
54-55	0.11556597513722663
56-57	0.09876827794880484
58-59	0.09040578800651693
60-61	0.09247823116612741
62-63	0.09118749902286123
64-65	0.0
66-67	1.817932596149546E-5
68-69	9.089662980747731E-5
70-71	0.0
72-73	0.0
74-75	0.0
76-77	7.271730384598184E-5
78-79	1.817932596149546E-5
80-81	1.6361393365345913E-4
82-83	1.4543460769196368E-4
84-85	0.0
86-87	5.272004528833684E-4
88-89	2.5451056346093644E-4
90-91	1.6361393365345913E-4
92-93	8.907869721132775E-4
94-95	8.362489942287912E-4
96-97	1.9997258557645004E-4
98-99	3.635865192299092E-5
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2750377.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.33631117552149
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.01531001193186	51.75247881406288
2	13.995422137833335	18.568093559361888
3	3.906770626962857	7.774822560047857
4	1.556497093237417	4.130091020831679
5	0.7432855880729993	2.4653412031345483
6	0.44281441919149445	1.7624805062696873
7	0.2710475527200536	1.2586206360420729
8	0.20212263602323574	1.0726456063083227
9	0.14173302901372795	0.8461841684853484
>10	0.6925976293036442	7.916072508160416
>50	0.025775321682192428	1.1570194716016269
>100	0.00607539014555516	0.6689733913000944
>500	3.2913832901304574E-4	0.12703645790145934
>1k	1.6456916445136945E-4	0.29941448733534526
>5k	5.485638815045648E-5	0.2007256091567531
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5516	0.20055432400721795	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4572	0.16623175659191447	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	3.635865192299092E-5	0.0	0.0	0.0	0.0
14-15	3.635865192299092E-5	0.0	0.0	0.0	0.0
16-17	7.271730384598184E-5	0.0	0.0	0.0	0.0
18-19	7.271730384598184E-5	0.0	0.0	0.0	0.0
20-21	7.271730384598184E-5	0.0	0.0	0.0	0.0
22-23	7.271730384598184E-5	0.0	0.0	9.089662980747731E-5	0.0
24-25	7.271730384598184E-5	0.0	0.0	1.8179325961495461E-4	0.0
26-27	7.271730384598184E-5	0.0	0.0	4.181244971143956E-4	0.0
28-29	7.271730384598184E-5	0.0	0.0	0.0011271182096127185	0.0
30-31	7.271730384598184E-5	0.0	0.0	0.004290320926912929	0.0
32-33	7.271730384598184E-5	0.0	0.0	0.01165294794131859	0.0
34-35	7.271730384598184E-5	0.0	0.0	0.02326953723071419	0.0
36-37	7.271730384598184E-5	0.0	0.0	0.044230300064318454	0.0
38-39	7.271730384598184E-5	0.0	0.0	0.0828977263844193	0.0
40-41	7.271730384598184E-5	0.0	0.0	0.13100022287853627	0.0
42-43	7.271730384598184E-5	0.0	0.0	0.17892092611303834	0.0
44-45	7.271730384598184E-5	0.0	0.0	0.23367705590906265	0.0
46-47	7.271730384598184E-5	0.0	0.0	0.3009587412925574	0.0
48-49	7.271730384598184E-5	0.0	0.0	0.3782390559548745	0.0
50-51	7.271730384598184E-5	0.0	0.0	0.46509987539889985	0.0
52-53	7.271730384598184E-5	0.0	0.0	0.5522697433842706	0.0
54-55	7.271730384598184E-5	0.0	0.0	0.6467113417542395	0.0
56-57	7.271730384598184E-5	0.0	0.0	0.7564235739318645	0.0
58-59	7.271730384598184E-5	0.0	0.0	0.8858240161257893	0.0
60-61	7.271730384598184E-5	0.0	0.0	1.025459418836036	0.0
62-63	7.271730384598184E-5	0.0	0.0	1.1703850054010778	0.0
64-65	7.271730384598184E-5	0.0	0.0	1.3234731093228311	0.0
66-67	7.271730384598184E-5	1.817932596149546E-5	0.0	1.4989399635031853	0.0
68-69	7.271730384598184E-5	3.635865192299092E-5	0.0	1.6951130699536825	0.0
70-71	7.271730384598184E-5	3.635865192299092E-5	0.0	1.9074657765099112	0.0
72-73	7.271730384598184E-5	3.635865192299092E-5	0.0	2.1353254481112955	0.0
74-75	7.271730384598184E-5	3.635865192299092E-5	0.0	2.358494853614614	0.0
76-77	7.271730384598184E-5	3.635865192299092E-5	0.0	2.5981529077650083	0.0
78-79	7.271730384598184E-5	3.635865192299092E-5	0.0	2.8732424682143574	0.0
80-81	7.271730384598184E-5	3.635865192299092E-5	0.0	3.1781824818924824	0.0
82-83	7.271730384598184E-5	3.635865192299092E-5	0.0	3.496902424649421	0.0
84-85	7.271730384598184E-5	3.635865192299092E-5	0.0	3.8050238203708076	0.0
86-87	7.271730384598184E-5	3.635865192299092E-5	0.0	4.126652455281585	0.0
88	7.271730384598184E-5	3.635865192299092E-5	0.0	4.3912161859992285	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4105	0.0	48.98547	1
GTATCAA	7715	0.0	41.53222	1
TCAACGC	8935	0.0	35.171234	4
ATCAACG	9045	0.0	34.7435	3
CAACGCA	9045	0.0	34.738438	5
AACGCAG	9425	0.0	33.486958	6
TATCAAC	9460	0.0	33.418865	2
ACGCAGA	11020	0.0	28.553755	7
CGCAGAG	11205	0.0	28.081297	8
GTACATG	8570	0.0	26.314621	1
TACATGG	8805	0.0	24.898323	2
ACCGTCG	115	1.371337E-4	24.538967	8
GCAGAGT	12670	0.0	24.467272	9
ACATGGG	8620	0.0	24.23156	3
GTGGTAT	2300	0.0	23.082737	1
TGGTATC	2230	0.0	21.518345	2
ACCGTTC	330	1.8189894E-12	21.378647	8
GAGTACT	8145	0.0	20.846611	12-13
CAGAGTA	12420	0.0	20.043451	10-11
AGAGTAC	11650	0.0	20.037737	10-11
>>END_MODULE