FastQCFastQC Report
Wed 25 May 2016
SRR1032929_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032929_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2014330
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22450.033.4980161
GTATCAA35150.030.487721
TCAACGC37000.028.201144
GTACATG73250.027.3990611
ATCAACG38050.027.2993973
CAACGCA39400.026.721235
TACATGG73700.026.2113722
AACGCAG39650.026.1971286
ACATGGG74350.025.2236143
TATCAAC43600.024.1477662
TACACCG3550.023.8313585
ACGCAGA47950.021.1718587
CATGGGG49900.020.7222824
CGCAGAG49250.020.5170678
TATACCG1656.86127E-519.9397055
ACACCGT4250.019.9061936
CCTATAC9100.019.6271673
GAGTACA55050.018.3567811
AGTACAT54400.017.4529112
GCAGAGT56600.017.0223889
Produced by FastQC (version 0.11.5)