##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032929_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2014330 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.400283965387995 33.0 31.0 34.0 30.0 34.0 2 31.405569097417008 33.0 31.0 34.0 28.0 34.0 3 31.591939255236234 34.0 31.0 34.0 30.0 34.0 4 34.925244622281355 37.0 35.0 37.0 33.0 37.0 5 35.02856830807266 37.0 35.0 37.0 33.0 37.0 6 35.070287887287584 37.0 35.0 37.0 33.0 37.0 7 35.01689395481376 37.0 35.0 37.0 33.0 37.0 8 35.03703067521211 37.0 35.0 37.0 33.0 37.0 9 36.65130440394573 39.0 38.0 39.0 33.0 39.0 10-11 36.64553524000536 39.0 38.0 39.0 33.0 39.0 12-13 36.58325423341756 39.0 38.0 39.0 33.0 39.0 14-15 38.02760272646488 41.0 39.0 41.0 33.0 41.0 16-17 38.038911449464585 41.0 39.0 41.0 33.0 41.0 18-19 38.03904697839977 41.0 39.0 41.0 33.0 41.0 20-21 38.08205457894188 41.0 39.0 41.0 33.5 41.0 22-23 38.06255951110295 41.0 39.0 41.0 33.0 41.0 24-25 38.09687290563115 41.0 39.0 41.0 33.0 41.0 26-27 38.081969687191275 41.0 39.0 41.0 33.0 41.0 28-29 38.05725576246196 41.0 39.0 41.0 33.0 41.0 30-31 38.00840552441755 41.0 39.0 41.0 33.0 41.0 32-33 37.96667328590648 41.0 38.5 41.0 33.0 41.0 34-35 37.89215769015007 41.0 38.0 41.0 33.0 41.0 36-37 37.78835816375668 40.5 38.0 41.0 32.5 41.0 38-39 37.680999141153634 40.0 38.0 41.0 32.0 41.0 40-41 37.58424190673822 40.0 38.0 41.0 32.0 41.0 42-43 37.43986908798459 40.0 38.0 41.0 31.5 41.0 44-45 37.27094641890852 40.0 38.0 41.0 31.0 41.0 46-47 37.124408612292925 40.0 37.0 41.0 31.0 41.0 48-49 36.97280981765649 40.0 37.0 41.0 30.5 41.0 50-51 36.80909508372511 40.0 36.5 41.0 30.0 41.0 52-53 36.640120288135506 40.0 36.0 41.0 30.0 41.0 54-55 36.45856240040112 40.0 35.5 41.0 30.0 41.0 56-57 36.257948548648926 39.0 35.0 41.0 30.0 41.0 58-59 36.03227127630527 39.0 35.0 41.0 30.0 41.0 60-61 35.77900865300124 39.0 35.0 41.0 29.0 41.0 62-63 35.51377331420373 38.0 35.0 40.0 29.0 41.0 64-65 35.221333892659096 38.0 35.0 40.0 29.0 41.0 66-67 34.912556036002044 37.0 34.0 40.0 28.5 41.0 68-69 34.59100867285896 37.0 34.0 39.5 28.0 41.0 70-71 34.2494963585907 36.0 34.0 39.0 28.0 41.0 72-73 33.96010112543625 36.0 34.0 39.0 28.0 41.0 74-75 33.69088307278351 35.0 34.0 38.5 27.0 40.0 76-77 33.3769067133985 35.0 34.0 37.0 27.0 39.5 78-79 33.02781346651244 35.0 34.0 37.0 26.5 39.0 80-81 32.72640778819756 35.0 33.0 36.5 26.0 39.0 82-83 32.43338653547333 35.0 33.0 36.0 26.0 37.5 84-85 32.29289242576937 35.0 33.0 36.0 26.0 37.0 86-87 32.08778948831622 35.0 33.0 35.5 26.0 37.0 88-89 31.8740084792462 35.0 33.0 35.0 25.5 36.5 90-91 31.688975987052764 35.0 33.0 35.0 25.0 36.0 92-93 31.509221428465047 35.0 33.0 35.0 25.0 36.0 94-95 31.333709719857225 35.0 33.0 35.0 24.5 36.0 96-97 31.16022672551171 35.0 33.0 35.0 24.0 35.5 98-99 30.98073602637105 35.0 33.0 35.0 23.0 35.0 100 30.84688010405445 35.0 33.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15181066669904553 1101 2 0.21502702010239716 1101 3 0.24718900077421324 1101 4 0.24326364223350794 1101 5 0.1690848292308118 1101 6 0.16142101960100774 1101 7 0.20071279492851346 1101 8 0.14682243140109108 1101 9 0.12737697479723664 1101 10-11 0.15233006247778036 1101 12-13 0.03898967374014717 1101 14-15 0.05667750345725153 1101 16-17 0.05421715420691697 1101 18-19 0.09330505164928127 1101 20-21 0.12049465922691383 1101 22-23 0.1317013781763947 1101 24-25 0.15686732133794834 1101 26-27 0.16366843597110403 1101 28-29 0.14195989395636843 1101 30-31 0.0919915238219815 1101 32-33 0.08625417599525775 1101 34-35 0.15522457675253776 1101 36-37 0.1313936855110427 1101 38-39 0.13624766803796717 1101 40-41 0.1428606676261026 1101 42-43 0.08954594011045458 1101 44-45 0.0772648781080747 1101 46-47 0.08936251203049039 1101 48-49 0.10885016655567625 1101 50-51 0.08535045001166708 1101 52-53 0.02405028706765222 1101 54-55 0.10230769825368924 1101 56-57 0.09804774573463249 1101 58-59 0.07817287929185568 1101 60-61 0.19280395749962764 1101 62-63 0.23973703927742918 1101 64-65 0.28943277027437375 1101 66-67 0.2180271804569216 1101 68-69 0.17941585510061486 1101 70-71 0.1888098478083009 1101 72-73 0.2059326381855655 1101 74-75 0.23424764638259177 1101 76-77 0.185817075189739 1101 78-79 0.24549332374897403 1101 80-81 0.17512085334530525 1101 82-83 0.12951959227530097 1101 84-85 0.09718618249274158 1101 86-87 0.15321349475092916 1101 88-89 0.17741229974470585 1101 90-91 0.08037440726279499 1101 92-93 0.17193080282730477 1101 94-95 0.13122520351937084 1101 96-97 0.09271063121912704 1101 98-99 0.052923461993437115 1101 100 0.057432350132401666 1102 1 0.2963502943814369 1102 2 0.2887877242203558 1102 3 0.30909611479321697 1102 4 0.26682242609758333 1102 5 0.35736540859993937 1102 6 0.2876037277248642 1102 7 0.2671996809020456 1102 8 0.28526555404359755 1102 9 0.35925597077481797 1102 10-11 0.27674093968926883 1102 12-13 0.3152774049067304 1102 14-15 0.4680719707517085 1102 16-17 0.5166057437277374 1102 18-19 0.3667185030176441 1102 20-21 0.3741254406228549 1102 22-23 0.3574380447524774 1102 24-25 0.4353266476878659 1102 26-27 0.45221258534572684 1102 28-29 0.48358996773891505 1102 30-31 0.4207805191548246 1102 32-33 0.44268325400377506 1102 34-35 0.4983184991100842 1102 36-37 0.421517842888953 1102 38-39 0.4713586935392229 1102 40-41 0.4065899927131795 1102 42-43 0.46348791687749724 1102 44-45 0.4160036211671425 1102 46-47 0.36012374525997615 1102 48-49 0.4163295016335269 1102 50-51 0.4247359293371531 1102 52-53 0.45335422034312245 1102 54-55 0.362462989555425 1102 56-57 0.27892888959595297 1102 58-59 0.22160766030202694 1102 60-61 0.3300972083704252 1102 62-63 0.3475784134541229 1102 64-65 0.3842312523157574 1102 66-67 0.3508403007176355 1102 68-69 0.3831929281068227 1102 70-71 0.31954781718163616 1102 72-73 0.22639827764055553 1102 74-75 0.2685983338279385 1102 76-77 0.28628911939853197 1102 78-79 0.22876370186923367 1102 80-81 0.23514943495875684 1102 82-83 0.1688371609625534 1102 84-85 0.2320057170983958 1102 86-87 0.27300903284348976 1102 88-89 0.2951541338168262 1102 90-91 0.16942712712515373 1102 92-93 0.2276991841243543 1102 94-95 0.20467952382419696 1102 96-97 0.2625763445259395 1102 98-99 0.26617858486400436 1102 100 0.29979260314681966 1103 1 0.11278403402222636 1103 2 0.11503761056480499 1103 3 0.1880835781719341 1103 4 0.10841422017944069 1103 5 0.09189087088195436 1103 6 0.08625689987997731 1103 7 0.0850832451503507 1103 8 0.07201080795329062 1103 9 0.17554644711447054 1103 10-11 0.2305033141364916 1103 12-13 0.2375765478483629 1103 14-15 0.2593500118368226 1103 16-17 0.27588143758692496 1103 18-19 0.24431096387875328 1103 20-21 0.15447427466333608 1103 22-23 0.27111566590420466 1103 24-25 0.2591840369033065 1103 26-27 0.3430357622660978 1103 28-29 0.29290753268558234 1103 30-31 0.2850226231820585 1103 32-33 0.35674049106722094 1103 34-35 0.3324655043212985 1103 36-37 0.3493498145205223 1103 38-39 0.3333724483442779 1103 40-41 0.36698762287075226 1103 42-43 0.3803067610062669 1103 44-45 0.3601510542580897 1103 46-47 0.38502252496227385 1103 48-49 0.3922786496562267 1103 50-51 0.39006926674553455 1103 52-53 0.435271972181539 1103 54-55 0.4666267432016369 1103 56-57 0.45936914472433443 1103 58-59 0.43934132656654157 1103 60-61 0.48646871952700366 1103 62-63 0.4923764784190041 1103 64-65 0.4966568413571508 1103 66-67 0.4866221375798361 1103 68-69 0.5026636332449641 1103 70-71 0.4298119132080416 1103 72-73 0.4350016145421023 1103 74-75 0.39192606906819094 1103 76-77 0.36495117769866425 1103 78-79 0.3607406564709379 1103 80-81 0.3623745050431637 1103 82-83 0.40891137007909606 1103 84-85 0.4288905616749119 1103 86-87 0.373397384632959 1103 88-89 0.3317647202234504 1103 90-91 0.3504043331061979 1103 92-93 0.2741771099875727 1103 94-95 0.33225638490362286 1103 96-97 0.35871416967950864 1103 98-99 0.3966587881680006 1103 100 0.3873024119075019 1104 1 0.09207667423200405 1104 2 0.18027588401789885 1104 3 0.2299949759645834 1104 4 0.24568856854154575 1104 5 0.2814389805449835 1104 6 0.16348797694154626 1104 7 0.2079233492558643 1104 8 0.277117506321261 1104 9 0.3622770300662168 1104 10-11 0.3202453985950271 1104 12-13 0.3284405918192377 1104 14-15 0.4103449582185732 1104 16-17 0.3393299694447691 1104 18-19 0.3937152190938136 1104 20-21 0.3909480183524252 1104 22-23 0.43482868105038364 1104 24-25 0.30280262081391385 1104 26-27 0.33639665078318615 1104 28-29 0.36175488699656455 1104 30-31 0.2541138524881532 1104 32-33 0.2611643639097778 1104 34-35 0.24206774591653613 1104 36-37 0.280981343129973 1104 38-39 0.374463733910396 1104 40-41 0.39484318625379444 1104 42-43 0.41355570429615796 1104 44-45 0.31437709971618943 1104 46-47 0.3085107812927532 1104 48-49 0.24065426362437137 1104 50-51 0.3248394934489838 1104 52-53 0.2390574771519951 1104 54-55 0.2585968471114626 1104 56-57 0.2862265818109506 1104 58-59 0.24525982046110784 1104 60-61 0.32582358822098456 1104 62-63 0.3117256477274424 1104 64-65 0.2891142060395495 1104 66-67 0.3084114021878648 1104 68-69 0.26024161453728567 1104 70-71 0.29664320161162294 1104 72-73 0.27145695141737036 1104 74-75 0.36994692686240427 1104 76-77 0.3179774780391611 1104 78-79 0.2822754667523384 1104 80-81 0.2970257907071385 1104 82-83 0.29032953864392397 1104 84-85 0.25321715358911945 1104 86-87 0.26992583508499735 1104 88-89 0.22584225143552672 1104 90-91 0.17232880524036176 1104 92-93 0.1583850421624362 1104 94-95 0.20203845067403847 1104 96-97 0.19407188562017552 1104 98-99 0.18840269007008104 1104 100 0.14039952650184517 1105 1 0.07108069097787251 1105 2 0.1431768514313383 1105 3 0.20822180895847353 1105 4 0.23411143004867085 1105 5 0.18669610885455512 1105 6 0.23406814213728921 1105 7 0.1606417244538747 1105 8 0.14478977380258584 1105 9 0.1858365175068144 1105 10-11 0.2220331333766552 1105 12-13 0.24584872997266416 1105 14-15 0.3316315898094331 1105 16-17 0.2583943080960509 1105 18-19 0.31900021004198464 1105 20-21 0.28954993620714475 1105 22-23 0.3286041392988537 1105 24-25 0.26233425975937763 1105 26-27 0.29545236191692226 1105 28-29 0.2624238731925388 1105 30-31 0.3315069278445648 1105 32-33 0.37208383098377595 1105 34-35 0.39608237026593685 1105 36-37 0.37104166503538494 1105 38-39 0.3293959159651578 1105 40-41 0.3563984023660396 1105 42-43 0.3943729140721217 1105 44-45 0.2776977746426468 1105 46-47 0.21685436864283503 1105 48-49 0.144277467403505 1105 50-51 0.1518404410615659 1105 52-53 0.2471917411257465 1105 54-55 0.18420996659551747 1105 56-57 0.18490346006394276 1105 58-59 0.12044545352288338 1105 60-61 0.16304213314065663 1105 62-63 0.14844704962832367 1105 64-65 0.23791130379922265 1105 66-67 0.2178168106513212 1105 68-69 0.1933995688255834 1105 70-71 0.2243347042140229 1105 72-73 0.1949206168394113 1105 74-75 0.2270883191457287 1105 76-77 0.17264482359245648 1105 78-79 0.2050369088206736 1105 80-81 0.18512830258764978 1105 82-83 0.20029715009448523 1105 84-85 0.2529470132858833 1105 86-87 0.22842782931446237 1105 88-89 0.2759836861492637 1105 90-91 0.20561723305208446 1105 92-93 0.2414333845475909 1105 94-95 0.1805131480037545 1105 96-97 0.18524070209449306 1105 98-99 0.21567589950660349 1105 100 0.15603725562608162 1106 1 -0.05153945695942852 1106 2 -0.03212789777594338 1106 3 0.11153363058305743 1106 4 0.017262363722267082 1106 5 0.02833489754632268 1106 6 0.0014983517742095387 1106 7 -0.07278280870932718 1106 8 -0.0016057199170305125 1106 9 -0.018132106479967547 1106 10-11 0.04012491666731677 1106 12-13 0.06430279061441979 1106 14-15 0.08784244817840658 1106 16-17 0.05446760331358291 1106 18-19 0.08301826460920125 1106 20-21 0.0837818536938002 1106 22-23 0.01059005989910844 1106 24-25 0.02744494009102283 1106 26-27 0.05564656711517557 1106 28-29 0.006308099847970539 1106 30-31 -0.03989551832844285 1106 32-33 0.007554089478716719 1106 34-35 0.033889198393112 1106 36-37 0.07688370256772004 1106 38-39 0.01563885104560825 1106 40-41 0.058782275945901574 1106 42-43 0.04910623977139039 1106 44-45 -0.015138880872832772 1106 46-47 -0.041110620983012325 1106 48-49 -0.06159013061927254 1106 50-51 -0.04680751123642324 1106 52-53 -0.07836847666326463 1106 54-55 -0.1391483001442282 1106 56-57 -0.11122572742600312 1106 58-59 -0.10596800751748248 1106 60-61 -0.09309374778971602 1106 62-63 -0.11455028935422007 1106 64-65 -0.038885291952425405 1106 66-67 0.006855177288272785 1106 68-69 -0.0048186264265694945 1106 70-71 0.040577271223405376 1106 72-73 -0.026905247377811747 1106 74-75 -0.04732842454321684 1106 76-77 -0.020108197998318644 1106 78-79 0.03591711668539688 1106 80-81 0.01215296607527705 1106 82-83 -0.015096331503329452 1106 84-85 -0.026426420708069998 1106 86-87 0.020806440841354856 1106 88-89 5.745138183961274E-4 1106 90-91 0.04788310577583843 1106 92-93 0.057401943099520736 1106 94-95 0.09674017423495229 1106 96-97 0.09541057370585193 1106 98-99 0.07107713786124137 1106 100 0.11801415166917906 1107 1 0.05351888410047323 1107 2 0.17822320797691305 1107 3 0.042837656365716015 1107 4 0.10433596880314866 1107 5 0.15863131758444382 1107 6 0.17527718194118336 1107 7 0.2210537540229609 1107 8 0.21897356692731762 1107 9 0.2032045153507127 1107 10-11 0.2227597507496668 1107 12-13 0.21083955900911633 1107 14-15 0.24693182508870848 1107 16-17 0.2721109037882101 1107 18-19 0.2787328867778385 1107 20-21 0.22194799110150853 1107 22-23 0.12991125135777537 1107 24-25 0.16463485956837332 1107 26-27 0.1839614398610081 1107 28-29 0.1989628479005745 1107 30-31 0.2640876915986823 1107 32-33 0.1711116333669409 1107 34-35 0.1809782084555991 1107 36-37 0.21287162635793067 1107 38-39 0.1356910020958466 1107 40-41 0.16272978606485822 1107 42-43 0.17306764020197818 1107 44-45 0.1600318259352136 1107 46-47 0.17560789905309093 1107 48-49 0.2204101180317508 1107 50-51 0.17533284876473232 1107 52-53 0.0708028762872317 1107 54-55 0.09378528726843172 1107 56-57 0.19638459176661627 1107 58-59 0.21280567014499496 1107 60-61 0.2374729051310922 1107 62-63 0.18597829106731467 1107 64-65 0.2628136555708309 1107 66-67 0.1577201370181882 1107 68-69 0.17381075922153855 1107 70-71 0.20449192263480853 1107 72-73 0.23061655555224547 1107 74-75 0.2126826246677993 1107 76-77 0.1938386073175593 1107 78-79 0.1510103651972159 1107 80-81 0.09494927010543108 1107 82-83 0.060525427387474906 1107 84-85 0.06746166706924583 1107 86-87 0.10836224504793535 1107 88-89 0.21070345338306495 1107 90-91 0.28835359904168456 1107 92-93 0.24300439213700287 1107 94-95 0.24629588563516336 1107 96-97 0.22423139437680817 1107 98-99 0.3035341076632747 1107 100 0.31083295248832954 1108 1 -0.17719460540238785 1108 2 -0.1749967166676214 1108 3 -0.18093315171322288 1108 4 -0.14426162426051548 1108 5 -0.20030373582176964 1108 6 -0.20748281711729533 1108 7 -0.22739870158675046 1108 8 -0.25219376188782405 1108 9 -0.28819694165615317 1108 10-11 -0.3015769378802631 1108 12-13 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74-75 -0.026084742080485057 2214 76-77 -0.016384865372344848 2214 78-79 0.0029206682373796866 2214 80-81 -0.09940681737424484 2214 82-83 -0.06263200494968402 2214 84-85 0.0397625132324535 2214 86-87 -0.015513937214144846 2214 88-89 0.029252662411639818 2214 90-91 0.011840565075218024 2214 92-93 0.05800877846585095 2214 94-95 -0.004547145147125065 2214 96-97 -0.16628493206215467 2214 98-99 -0.08451471244475428 2214 100 -0.05663736009512377 2215 1 -0.239477072753715 2215 2 -0.2827927720755916 2215 3 -0.3807225393386986 2215 4 -0.29474861843675626 2215 5 -0.319496606544611 2215 6 -0.3282262226503718 2215 7 -0.31427800485283086 2215 8 -0.3396848030833084 2215 9 -0.4509585709354553 2215 10-11 -0.3616781777358966 2215 12-13 -0.36710145742951283 2215 14-15 -0.3640608802158596 2215 16-17 -0.47548610132131586 2215 18-19 -0.37138923740776875 2215 20-21 -0.3704859688484632 2215 22-23 -0.36725465228294496 2215 24-25 -0.39929317542054577 2215 26-27 -0.35013906847112963 2215 28-29 -0.3828414309407364 2215 30-31 -0.48433912469604223 2215 32-33 -0.5308379315827167 2215 34-35 -0.5032015686818667 2215 36-37 -0.45114019044450515 2215 38-39 -0.4557483672767688 2215 40-41 -0.42281355646555596 2215 42-43 -0.39817565352242923 2215 44-45 -0.46932345444815127 2215 46-47 -0.49102519301627723 2215 48-49 -0.4436984190875606 2215 50-51 -0.4607873035274608 2215 52-53 -0.4460702241093628 2215 54-55 -0.41082962756856034 2215 56-57 -0.3957497444736049 2215 58-59 -0.3978997858271569 2215 60-61 -0.3488854175293383 2215 62-63 -0.3674044997405588 2215 64-65 -0.38345639774453844 2215 66-67 -0.40672261747452865 2215 68-69 -0.3315548981690739 2215 70-71 -0.35368257282726034 2215 72-73 -0.33942605961954087 2215 74-75 -0.3349150537534413 2215 76-77 -0.373410947113868 2215 78-79 -0.33451843743984 2215 80-81 -0.2987866745126766 2215 82-83 -0.3624001265387875 2215 84-85 -0.4053097894641198 2215 86-87 -0.4006266482205092 2215 88-89 -0.4165849420350689 2215 90-91 -0.4129507695138557 2215 92-93 -0.38050326705663196 2215 94-95 -0.3659084167902513 2215 96-97 -0.386288900875833 2215 98-99 -0.4352939223394934 2215 100 -0.4367446721625967 2216 1 0.046392583122653264 2216 2 0.04944635057882607 2216 3 -0.05261224713877155 2216 4 -0.03484853825200673 2216 5 -0.01131643843466179 2216 6 -0.06953200279235716 2216 7 -0.08942113043397626 2216 8 -0.1721902520715517 2216 9 -0.1873338938037321 2216 10-11 -0.24156621476297602 2216 12-13 -0.23902033535496514 2216 14-15 -0.2958618111003162 2216 16-17 -0.2574171283171438 2216 18-19 -0.2531047099748349 2216 20-21 -0.19450416906496315 2216 22-23 -0.1872387918547176 2216 24-25 -0.17870745165942736 2216 26-27 -0.22521491741665756 2216 28-29 -0.1926557438960259 2216 30-31 -0.1888546909958535 2216 32-33 -0.3240086138856242 2216 34-35 -0.2692165632736945 2216 36-37 -0.22053104232249154 2216 38-39 -0.20446241046355595 2216 40-41 -0.18252155953878457 2216 42-43 -0.13328377495311372 2216 44-45 -0.1443947359080866 2216 46-47 -0.11765961367596844 2216 48-49 -0.07956103618177934 2216 50-51 -0.07266510497378675 2216 52-53 -0.10301079428161586 2216 54-55 -0.08196702415472146 2216 56-57 -0.06922571574830272 2216 58-59 -0.002086449034003124 2216 60-61 -0.05883750526742659 2216 62-63 0.00458432470338721 2216 64-65 -0.020482179104519105 2216 66-67 -0.11582972037799522 2216 68-69 -0.08803796956703991 2216 70-71 -0.010517671593063938 2216 72-73 -0.04136342372581936 2216 74-75 -0.07449710455005487 2216 76-77 -0.07657049975623664 2216 78-79 -4.604846530753548E-4 2216 80-81 -0.023242514870446485 2216 82-83 -0.02373530187065853 2216 84-85 -0.10040486156639616 2216 86-87 -0.027453476968929635 2216 88-89 -0.014392571015786615 2216 90-91 -0.020929549019925275 2216 92-93 0.026398468455504087 2216 94-95 0.03638137185652113 2216 96-97 0.05725146091315381 2216 98-99 0.048451345237932486 2216 100 -0.017427429617637102 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 33609.0 3 4994.0 4 3339.0 5 2964.0 6 2221.0 7 2490.0 8 2705.0 9 2981.0 10 3266.0 11 3423.0 12 3666.0 13 4119.0 14 4467.0 15 4665.0 16 4713.0 17 4699.0 18 4558.0 19 4305.0 20 4248.0 21 4544.0 22 5135.0 23 7421.0 24 11444.0 25 15003.0 26 15322.0 27 13440.0 28 15008.0 29 18636.0 30 22669.0 31 27865.0 32 35856.0 33 48981.0 34 72084.0 35 115302.0 36 219100.0 37 432131.0 38 610302.0 39 222430.0 40 225.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.18860221111679 18.94358636526004 12.919627052273194 23.948184371349974 2 15.735350595525253 19.507979337834943 39.94875695624071 24.8079131103991 3 18.653828478023794 24.89137968297027 29.188545990674424 27.266245848331504 4 12.517103941284665 16.135835849860932 37.53710201352018 33.80995819533421 5 13.357579009603871 37.99883739994932 34.78683181549438 13.856751774952428 6 31.96648740818468 36.98468154790295 17.30395839295367 13.744872650958694 7 28.74671780718468 32.084630270450205 21.427446460200077 17.74120546216504 8 26.23668372117518 36.339070391829736 19.316839248753702 18.107406638241383 9 25.763140989493564 17.095520743334923 20.069536116202222 37.07180215096928 10-11 24.797481492006824 26.820381174485625 27.64963267204199 20.732504661465565 12-13 25.090905477679158 24.060974610059098 27.96043332563264 22.88768658662911 14-15 22.44183259163393 25.955786186878445 24.798983521977114 26.803397699510516 16-17 22.560752956606628 29.263040492034985 25.46805175707263 22.708154794285758 18-19 22.532430578825874 28.212163041299355 27.19091310371004 22.064493276164733 20-21 23.37459908350589 27.54865148979973 26.862324182964976 22.21442524372941 22-23 23.322099679037265 27.21411749158718 26.783633459116814 22.680149370258746 24-25 22.98955133305396 27.278713837484887 26.84966841634993 22.882066413111225 26-27 22.74874040946287 27.326289777736267 26.997528139735742 22.92744167306512 28-29 22.91719088008986 27.407173549687236 26.954054437385384 22.721581132837525 30-31 22.895677846213847 27.5975462378172 26.7096410397811 22.79713487618785 32-33 22.69010598117353 27.462202366536133 27.079090298245156 22.76860135404518 34-35 22.9215652278455 27.26177530067002 27.03419557945891 22.78246389202557 36-37 23.080931672513714 27.495799763733604 26.637446335834102 22.78582222791858 38-39 22.912217375299374 27.696512749129266 26.739787805128863 22.651482070442498 40-41 22.76772440496787 27.38224612256324 27.01538654142751 22.834642931041376 42-43 22.788467748746765 27.41619841116063 26.909091360748015 22.88624247934459 44-45 22.713206852548193 27.472296256476465 27.015065560164736 22.799431330810606 46-47 22.779951746506192 27.510354229931476 26.941542323163265 22.768151700399063 48-49 22.75967026376812 27.43810489610904 26.925643396555465 22.876581443567375 50-51 22.688743064378368 27.47107373136568 26.9262855277482 22.913897676507748 52-53 22.83775088628476 27.31118470248905 26.933790118825506 22.917274292400684 54-55 22.752951628177826 27.613287767474997 26.892833115871962 22.74092748847521 56-57 22.621608495235776 27.475141696985204 26.944077668455762 22.959172139323258 58-59 22.702840075312686 27.040027068446946 27.205577848987577 23.051555007252784 60-61 22.89962821584845 27.496476173867595 26.80651673078366 22.7973788795003 62-63 22.646505502891742 27.53825741306082 26.89646246400073 22.918774620046708 64-65 22.712731178778256 27.28602672591627 26.980123494083653 23.021118601221822 66-67 22.574912314652217 27.377354164342922 26.924637179283028 23.12309634172183 68-69 22.829251303382023 27.476835733497225 26.86130802990643 22.832604933214327 70-71 22.758557986986677 27.2541482976187 26.971008059237366 23.016285656157258 72-73 22.595319875883632 27.359109017166983 27.083868970833247 22.961702136116138 74-75 22.65526858305704 27.520966497076575 26.938343680887446 22.885421238978942 76-77 22.806518106929026 27.36083640990405 26.794583446981385 23.03806203618554 78-79 22.70512236109783 27.418375217514967 26.89855014861194 22.977952272775262 80-81 22.6543777702867 27.426319658201614 26.94971172747148 22.9695908440402 82-83 22.67776177998766 27.384440807679734 27.01221893323556 22.92557847909705 84-85 22.680419029572583 27.353501489348016 26.906386571697492 23.059692909381912 86-87 22.820637536460783 27.40521935095706 26.921062868799805 22.853080243782355 88-89 22.53400912739301 27.39877248189376 26.92936156936312 23.137856821350113 90-91 22.641047321633497 27.337342131227192 26.9152906295674 23.10631991757191 92-93 22.801167163334558 27.39848115754544 26.893790074044375 22.906561605075627 94-95 22.61952341906617 27.250427927262177 26.96300686496923 23.167041788702424 96-97 22.70225250221445 27.22732730659039 26.97572726545605 23.094692925739114 98-99 22.64586382888033 27.381546456770366 26.904704360135632 23.067885354213672 100 22.673793869181722 27.154889974639147 26.93908676093982 23.23222939523931 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2231.0 1 1537.0 2 747.5 3 570.0 4 458.0 5 392.0 6 324.0 7 298.0 8 292.5 9 286.5 10 268.5 11 259.5 12 269.5 13 300.5 14 336.5 15 359.5 16 391.0 17 476.0 18 664.0 19 864.0 20 1136.5 21 1650.0 22 2355.5 23 3445.5 24 4998.5 25 6907.0 26 9580.0 27 12745.0 28 16522.5 29 21072.5 30 25513.5 31 29851.0 32 34144.0 33 38680.0 34 43125.0 35 47265.0 36 51713.5 37 55898.0 38 59402.0 39 61956.5 40 64701.0 41 67991.0 42 69874.5 43 70742.0 44 71219.0 45 71524.0 46 72104.5 47 71166.5 48 70208.0 49 69207.5 50 67282.5 51 65553.0 52 61948.5 53 58742.5 54 57187.5 55 54915.0 56 51650.5 57 47229.0 58 43206.5 59 40943.5 60 37837.5 61 33279.5 62 29338.0 63 25727.0 64 21586.0 65 17705.5 66 14463.5 67 11408.5 68 8892.5 69 6933.5 70 5166.0 71 3903.5 72 3115.5 73 2387.0 74 1840.5 75 1411.5 76 1019.0 77 757.0 78 542.0 79 348.5 80 246.5 81 171.0 82 141.0 83 120.0 84 84.5 85 68.5 86 53.5 87 41.5 88 39.0 89 26.0 90 21.5 91 18.0 92 12.0 93 13.0 94 12.5 95 6.5 96 5.0 97 5.0 98 4.0 99 2.0 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07451609219939137 2 0.07789190450422721 3 0.07868621328183566 4 0.08121807251046254 5 0.07943087776084355 6 0.07913301196924039 7 0.07818977029583038 8 0.07784226020562669 9 0.07759403871262405 10-11 0.07699830712941771 12-13 0.07570755536580402 14-15 0.07434233715428952 16-17 0.07272889744977239 18-19 0.07215798801586631 20-21 0.07158707858196026 22-23 0.07344873977948002 24-25 0.0739451827654853 26-27 0.07315087398787687 28-29 0.06917933009983468 30-31 0.07459055864729215 32-33 0.07310122968927633 34-35 0.06994881672814286 36-37 0.06692051451351069 38-39 0.06796304478412177 40-41 0.07121474634245631 42-43 0.07645221984481193 44-45 0.07746992796612273 46-47 0.08072162952445726 48-49 0.08394850893349153 50-51 0.08504068350270313 52-53 0.08513997209990419 54-55 0.0849910392041026 56-57 0.08303008940938178 58-59 0.0821364920345723 60-61 0.07957981065664514 62-63 0.08020036438915173 64-65 0.07953016635804461 66-67 0.07910818981994013 68-69 0.07871103543113592 70-71 0.07677490778571536 72-73 0.07652668629271271 74-75 0.07622882050110955 76-77 0.07630328694901034 78-79 0.0763281090983106 80-81 0.07766850516052484 82-83 0.07704795142801825 84-85 0.07627846479971008 86-87 0.07699830712941771 88-89 0.0738707163175845 90-91 0.07069348120715077 92-93 0.07027150466904629 94-95 0.07096652484945366 96-97 0.07014739392254497 98-99 0.06947719589143785 100 0.06875735356173021 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2014330.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.04028269478412 #Duplication Level Percentage of deduplicated Percentage of total 1 79.19105136950974 50.71417316600575 2 12.4935525209147 16.0018127060302 3 3.6605313789127596 7.032643929561033 4 1.5885043692906213 4.069130754850846 5 0.8056893365250299 2.5798286437617985 6 0.492776069892753 1.8934511292693963 7 0.34219374427414395 1.5339928883791991 8 0.24213291979588583 1.2405008506753616 9 0.18623342276205013 1.0733796936809041 >10 0.9379519392585146 10.56487998827302 >50 0.048207957031043996 2.076877895262234 >100 0.01094238751866264 1.0642738482460012 >500 7.752810470425863E-5 0.02944198593036567 >1k 1.5505620940851672E-4 0.12561252007390367 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 4.964429860052722E-5 0.0 0.0 0.0 3 0.0 4.964429860052722E-5 0.0 0.0 0.0 4 0.0 4.964429860052722E-5 0.0 0.0 0.0 5 0.0 4.964429860052722E-5 0.0 0.0 0.0 6 0.0 4.964429860052722E-5 0.0 0.0 0.0 7 0.0 4.964429860052722E-5 0.0 0.0 0.0 8 0.0 4.964429860052722E-5 0.0 0.0 0.0 9 0.0 4.964429860052722E-5 0.0 0.0 0.0 10-11 0.0 9.928859720105445E-5 0.0 0.0 0.0 12-13 0.0 1.4893289580158168E-4 0.0 0.0 0.0 14-15 0.0 1.4893289580158168E-4 0.0 0.0 0.0 16-17 0.0 1.4893289580158168E-4 0.0 2.482214930026361E-5 0.0 18-19 0.0 1.4893289580158168E-4 0.0 4.964429860052722E-5 0.0 20-21 0.0 1.4893289580158168E-4 0.0 9.928859720105444E-5 0.0 22-23 0.0 1.4893289580158168E-4 0.0 9.928859720105444E-5 0.0 24-25 0.0 1.4893289580158168E-4 0.0 1.9857719440210888E-4 0.0 26-27 0.0 1.9857719440210888E-4 0.0 3.2268794090342694E-4 0.0 28-29 0.0 2.4822149300263613E-4 0.0 0.0016382618538173983 0.0 30-31 0.0 2.4822149300263613E-4 0.0 0.005163007054454831 0.0 32-33 0.0 2.4822149300263613E-4 0.0 0.01347842707004314 0.0 34-35 0.0 2.4822149300263613E-4 0.0 0.02482214930026361 0.0 36-37 0.0 2.9786579160316336E-4 0.0 0.04602026480268873 0.0 38-39 0.0 3.7233223950395417E-4 0.0 0.08332795520098495 0.0 40-41 0.0 3.9715438880421776E-4 0.0 0.13046521672218553 0.0 42-43 0.0 4.964429860052723E-4 0.0 0.17670888086857667 0.0 44-45 0.0 4.964429860052723E-4 0.0 0.23178923016586161 0.0 46-47 0.0 5.460872846057994E-4 0.0 0.29669915058605095 0.0 48-49 0.0 5.957315832063267E-4 0.0 0.3706691555008365 0.0 50-51 0.0 5.957315832063267E-4 0.0 0.45320280192421303 0.0 52-53 0.0 5.957315832063267E-4 0.0 0.5377222202916105 0.0 54-55 0.0 5.957315832063267E-4 0.0 0.627876266550168 0.0 56-57 0.0 5.957315832063267E-4 0.0 0.730143521667254 0.0 58-59 0.0 5.957315832063267E-4 0.0 0.856314506560494 0.0 60-61 0.0 5.957315832063267E-4 0.0 0.9941270794755577 0.0 62-63 0.0 6.453758818068539E-4 0.0 1.130971588567911 0.0 64-65 0.0 6.453758818068539E-4 0.0 1.2727060610724161 0.0 66-67 0.0 6.453758818068539E-4 0.0 1.4352911389891427 0.0 68-69 0.0 6.950201804073811E-4 0.0 1.6236167857302428 0.0 70-71 0.0 6.950201804073811E-4 0.0 1.8298640242661333 0.0 72-73 0.0 6.950201804073811E-4 0.0 2.0454940352375233 0.0 74-75 0.0 6.950201804073811E-4 0.0 2.255390129720552 0.0 76-77 0.0 7.943087776084355E-4 0.0 2.4762079698956976 0.0 78-79 0.0 7.943087776084355E-4 0.0 2.740762437137907 0.0 80-81 0.0 7.943087776084355E-4 0.0 3.029741899291576 0.0 82-83 0.0 7.943087776084355E-4 0.0 3.3321749663659874 0.0 84-85 0.0 7.943087776084355E-4 0.0 3.6297180700282476 0.0 86-87 0.0 7.943087776084355E-4 0.0 3.9309348517869465 0.0 88 0.0 7.943087776084355E-4 0.0 4.1785109689077755 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2245 0.0 33.498016 1 GTATCAA 3515 0.0 30.48772 1 TCAACGC 3700 0.0 28.20114 4 GTACATG 7325 0.0 27.399061 1 ATCAACG 3805 0.0 27.299397 3 CAACGCA 3940 0.0 26.72123 5 TACATGG 7370 0.0 26.211372 2 AACGCAG 3965 0.0 26.197128 6 ACATGGG 7435 0.0 25.223614 3 TATCAAC 4360 0.0 24.147766 2 TACACCG 355 0.0 23.831358 5 ACGCAGA 4795 0.0 21.171858 7 CATGGGG 4990 0.0 20.722282 4 CGCAGAG 4925 0.0 20.517067 8 TATACCG 165 6.86127E-5 19.939705 5 ACACCGT 425 0.0 19.906193 6 CCTATAC 910 0.0 19.627167 3 GAGTACA 5505 0.0 18.356781 1 AGTACAT 5440 0.0 17.452911 2 GCAGAGT 5660 0.0 17.022388 9 >>END_MODULE