FastQCFastQC Report
Wed 25 May 2016
SRR1032929_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032929_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2014330
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55410.27507905854552134No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45300.2248886726603883No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24500.12162853157129169No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36600.048.9077451
GTATCAA63150.040.1004451
TCAACGC74300.033.495264
ATCAACG74600.033.4244543
CAACGCA76450.032.609145
AACGCAG77250.032.3323256
TATCAAC78200.032.0662162
GTACATG76650.027.8890061
TACATGG76100.027.2634752
ACGCAGA92050.026.9785527
CGCAGAG94350.026.4199318
ACATGGG78550.025.63453
GCAGAGT103850.023.925099
TATACCG1452.5190726E-522.7075845
GAGTACT64100.021.17601412-13
CATGGGG55500.020.5134584
CAGAGTA101600.020.36369510-11
GTGGTAT18800.019.742541
AGAGTAC95900.019.54153610-11
GAGTACA56550.019.3578471
Produced by FastQC (version 0.11.5)