##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032929_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2014330 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.822732620772165 34.0 31.0 34.0 31.0 34.0 2 32.874157660363494 34.0 31.0 34.0 31.0 34.0 3 32.94450015637954 34.0 31.0 34.0 31.0 34.0 4 36.364490426097014 37.0 37.0 37.0 35.0 37.0 5 36.3450725551424 37.0 37.0 37.0 35.0 37.0 6 36.40623383457527 37.0 37.0 37.0 35.0 37.0 7 36.37141381997984 37.0 37.0 37.0 35.0 37.0 8 36.31263596332279 37.0 37.0 37.0 35.0 37.0 9 38.151813258006385 39.0 39.0 39.0 37.0 39.0 10-11 38.15779266555133 39.0 39.0 39.0 37.0 39.0 12-13 38.147956888891095 39.0 39.0 39.0 37.0 39.0 14-15 39.70462089131374 41.0 40.0 41.0 37.0 41.0 16-17 39.71172921020886 41.0 40.0 41.0 37.0 41.0 18-19 39.734040350885905 41.0 40.0 41.0 37.0 41.0 20-21 39.7395813992742 41.0 40.0 41.0 37.0 41.0 22-23 39.70196442489562 41.0 40.0 41.0 37.0 41.0 24-25 39.67639512890142 41.0 40.0 41.0 37.0 41.0 26-27 39.59809589292718 41.0 40.0 41.0 37.0 41.0 28-29 39.53299161507797 41.0 40.0 41.0 36.5 41.0 30-31 39.417673370301785 41.0 39.0 41.0 36.0 41.0 32-33 39.28590970694971 41.0 39.0 41.0 35.5 41.0 34-35 39.189960433494015 41.0 39.0 41.0 35.0 41.0 36-37 39.099505542785934 41.0 39.0 41.0 35.0 41.0 38-39 38.99983418804267 41.0 39.0 41.0 35.0 41.0 40-41 38.89446540537052 41.0 39.0 41.0 35.0 41.0 42-43 38.74416729135743 41.0 39.0 41.0 35.0 41.0 44-45 38.55523772172385 41.0 38.0 41.0 35.0 41.0 46-47 38.314464859283234 41.0 38.0 41.0 34.5 41.0 48-49 38.08756608897251 40.0 38.0 41.0 34.0 41.0 50-51 37.91031782279964 40.0 38.0 41.0 34.0 41.0 52-53 37.75282699458381 40.0 37.0 41.0 33.5 41.0 54-55 37.586338633689614 40.0 37.0 41.0 33.0 41.0 56-57 37.36223161051069 40.0 36.5 41.0 33.0 41.0 58-59 37.17926531402502 40.0 36.0 41.0 33.0 41.0 60-61 36.96576702923553 39.0 35.0 41.0 33.0 41.0 62-63 36.716575238416745 39.0 35.0 41.0 32.5 41.0 64-65 36.415565225161714 39.0 35.0 41.0 32.0 41.0 66-67 36.128181330765074 37.5 35.0 40.5 32.0 41.0 68-69 35.826231799159025 37.0 35.0 40.0 31.0 41.0 70-71 35.50309606668222 37.0 35.0 39.5 31.0 41.0 72-73 35.163844305550725 36.0 35.0 39.0 31.0 41.0 74-75 34.82774669493082 36.0 35.0 39.0 31.0 41.0 76-77 34.48813699840642 35.0 35.0 37.5 30.5 40.0 78-79 34.16418312788868 35.0 34.5 37.0 30.0 39.0 80-81 33.85416292265915 35.0 34.0 37.0 30.0 39.0 82-83 33.56658342972601 35.0 34.0 36.0 30.0 38.5 84-85 33.30965730540676 35.0 34.0 36.0 30.0 37.0 86-87 33.184432540844846 35.0 34.0 36.0 30.0 37.0 88-89 33.015878480685885 35.0 34.0 35.0 30.0 36.5 90-91 32.85831591645858 35.0 34.0 35.0 29.5 36.0 92-93 32.709450040460105 35.0 34.0 35.0 29.0 36.0 94-95 32.61970233278559 35.0 34.0 35.0 29.0 36.0 96-97 32.56371101060898 35.0 34.0 35.0 29.0 36.0 98-99 32.456150183932124 35.0 34.0 35.0 29.0 35.0 100 32.37542458286378 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16984875563747437 1101 2 0.20526766313130906 1101 3 0.1773692834255911 1101 4 0.10943894368745788 1101 5 0.07755197901824573 1101 6 0.10718036183322965 1101 7 0.0642313434258881 1101 8 0.04758577901451133 1101 9 0.04796543781269946 1101 10-11 0.17569666701460562 1101 12-13 0.19055949042544995 1101 14-15 0.22196264478187544 1101 16-17 0.22063116722500808 1101 18-19 0.21866418856335912 1101 20-21 0.22689964454090727 1101 22-23 0.2235982002268173 1101 24-25 0.24543187537604183 1101 26-27 0.26324116767196415 1101 28-29 0.26549474436203724 1101 30-31 0.2704835829660368 1101 32-33 0.32030359433483824 1101 34-35 0.2731897654063573 1101 36-37 0.2870857479716378 1101 38-39 0.31934384172237174 1101 40-41 0.2906088942915659 1101 42-43 0.26910061034107713 1101 44-45 0.29354283873611564 1101 46-47 0.29327842848001495 1101 48-49 0.28883253748488613 1101 50-51 0.22252560442124292 1101 52-53 0.2961375464783558 1101 54-55 0.2958931890941585 1101 56-57 0.3170014118657676 1101 58-59 0.36138204908726834 1101 60-61 0.4211042562484053 1101 62-63 0.33765260819635046 1101 64-65 0.36084304019302493 1101 66-67 0.33401547356479 1101 68-69 0.2995833563263588 1101 70-71 0.3810642489116276 1101 72-73 0.35468414915942503 1101 74-75 0.36455025997850754 1101 76-77 0.28950049892535645 1101 78-79 0.3476429594837782 1101 80-81 0.2657983468422813 1101 82-83 0.26423398003147724 1101 84-85 0.2896184256427645 1101 86-87 0.2520139314828498 1101 88-89 0.270020554985102 1101 90-91 0.2432473008011442 1101 92-93 0.22440609892158392 1101 94-95 0.23459242860555207 1101 96-97 0.1871818966195704 1101 98-99 0.22672560759382065 1101 100 0.21822357354787414 1102 1 0.12960084584113218 1102 2 0.18762155401924474 1102 3 0.17952702473215254 1102 4 0.07797039545118878 1102 5 0.0951093700348622 1102 6 0.05584402220863893 1102 7 0.07250796911171875 1102 8 -0.10054813042695798 1102 9 0.016269099871884407 1102 10-11 0.08769084732067256 1102 12-13 0.1567704178306002 1102 14-15 0.19026217732010053 1102 16-17 0.20155038260536884 1102 18-19 0.15326629722996898 1102 20-21 0.16122813914135037 1102 22-23 0.1910354621466155 1102 24-25 0.1900821742201515 1102 26-27 0.15790867729374725 1102 28-29 0.2118470718932599 1102 30-31 0.17445516973046438 1102 32-33 0.24053124017219574 1102 34-35 0.24414324781806584 1102 36-37 0.2864252910388103 1102 38-39 0.2507624893602127 1102 40-41 0.29863894761300713 1102 42-43 0.23310237724321325 1102 44-45 0.2674061681104405 1102 46-47 0.38362875039614863 1102 48-49 0.3790581745101207 1102 50-51 0.40228192005731245 1102 52-53 0.4561988445546348 1102 54-55 0.45900234005931395 1102 56-57 0.3615380289941754 1102 58-59 0.222562340775319 1102 60-61 0.31056763698863676 1102 62-63 0.39064821598457655 1102 64-65 0.42086989007187725 1102 66-67 0.4213894765519015 1102 68-69 0.4501531502925715 1102 70-71 0.3858947227515088 1102 72-73 0.3482679392710537 1102 74-75 0.338084426569246 1102 76-77 0.3836465166551264 1102 78-79 0.3766666981016229 1102 80-81 0.4267813273546679 1102 82-83 0.3263385117944182 1102 84-85 0.2902401183627106 1102 86-87 0.28571082251305313 1102 88-89 0.24362026106977908 1102 90-91 0.26962161869752777 1102 92-93 0.3800690575448442 1102 94-95 0.335477824541357 1102 96-97 0.3998955194030174 1102 98-99 0.39391952083978055 1102 100 0.3932329382357267 1103 1 0.17415203948549163 1103 2 0.22409104199238783 1103 3 0.23305681598542805 1103 4 0.09422333043868747 1103 5 0.13956912985680248 1103 6 0.12835155276447097 1103 7 0.17534605967806982 1103 8 0.07786256126863123 1103 9 0.10021833805495817 1103 10-11 0.18817050558740078 1103 12-13 0.18302994420340468 1103 14-15 0.2722516372161863 1103 16-17 0.25985014138817775 1103 18-19 0.2755042126203193 1103 20-21 0.21494105272977038 1103 22-23 0.23770415105437337 1103 24-25 0.18853779583388786 1103 26-27 0.23709972737427165 1103 28-29 0.22107781291218487 1103 30-31 0.2761137894566943 1103 32-33 0.33763911705592164 1103 34-35 0.296960097212299 1103 36-37 0.23634095034161362 1103 38-39 0.271497209787924 1103 40-41 0.22184071302437758 1103 42-43 0.21405421606906572 1103 44-45 0.19425439840753 1103 46-47 0.24384420057274525 1103 48-49 0.25398829385544985 1103 50-51 0.20293071816099228 1103 52-53 0.24895268865920883 1103 54-55 0.2126975144674219 1103 56-57 0.2635023152997036 1103 58-59 0.1487908458679712 1103 60-61 0.14954306115625116 1103 62-63 0.21919426284708265 1103 64-65 0.2564846755576937 1103 66-67 0.24314898165805943 1103 68-69 0.2306567723708568 1103 70-71 0.3057881246678491 1103 72-73 0.21685525105810655 1103 74-75 0.2214265849219288 1103 76-77 0.2270224163804997 1103 78-79 0.24759728811465465 1103 80-81 0.296953449615188 1103 82-83 0.2887187786945873 1103 84-85 0.25563063374638517 1103 86-87 0.2786464136250757 1103 88-89 0.2640636794518443 1103 90-91 0.2238263265460887 1103 92-93 0.21086847695280397 1103 94-95 0.20875468539156117 1103 96-97 0.1263955625730233 1103 98-99 0.14925585595314317 1103 100 0.13814721839048616 1104 1 0.11532104950466504 1104 2 0.13604813271290794 1104 3 0.12582507848004099 1104 4 0.06709804669245756 1104 5 0.07455773108571861 1104 6 0.06961767248581197 1104 7 0.09834100116763977 1104 8 0.08792650201459651 1104 9 0.09308053033706187 1104 10-11 0.12388443312448771 1104 12-13 0.15429843663031306 1104 14-15 0.16067485206100685 1104 16-17 0.19566417508548284 1104 18-19 0.20603842605618894 1104 20-21 0.1938169406597865 1104 22-23 0.1357017220887684 1104 24-25 0.13840631859228836 1104 26-27 0.19019733118625481 1104 28-29 0.24660017505064502 1104 30-31 0.2621901661151398 1104 32-33 0.246993264153879 1104 34-35 0.23752681916055707 1104 36-37 0.2502851819763876 1104 38-39 0.23413724833827132 1104 40-41 0.21010707246236393 1104 42-43 0.22430357030177817 1104 44-45 0.31781628199330214 1104 46-47 0.31114629980564956 1104 48-49 0.32715146506205883 1104 50-51 0.38291015878953516 1104 52-53 0.3611556112454011 1104 54-55 0.30654052697182976 1104 56-57 0.361367430657225 1104 58-59 0.3488092526817965 1104 60-61 0.3311633377599108 1104 62-63 0.2629280575126529 1104 64-65 0.36112808481760084 1104 66-67 0.4230265427124209 1104 68-69 0.40120611486235447 1104 70-71 0.3033929150870094 1104 72-73 0.33788365992663927 1104 74-75 0.3129947965379074 1104 76-77 0.3803038437218902 1104 78-79 0.29809317134818514 1104 80-81 0.2784138743539515 1104 82-83 0.34330494949025336 1104 84-85 0.365396029425618 1104 86-87 0.3214325325636622 1104 88-89 0.25982522009605447 1104 90-91 0.3157761890759261 1104 92-93 0.3674187954334869 1104 94-95 0.39799477139602857 1104 96-97 0.3856443444210953 1104 98-99 0.45625344166407444 1104 100 0.4492238396577548 1105 1 -0.016659460791778713 1105 2 0.08427100553480926 1105 3 0.05130949237459248 1105 4 0.0501294169119646 1105 5 0.003858760730430788 1105 6 -0.06065572081583781 1105 7 0.033650177451868046 1105 8 0.027354257162819806 1105 9 0.06201368829135134 1105 10-11 0.05741117842885046 1105 12-13 0.10133255068326719 1105 14-15 0.08892325384873345 1105 16-17 0.11826049212553613 1105 18-19 0.11774108785750315 1105 20-21 0.11600486496393358 1105 22-23 0.12450751808334104 1105 24-25 0.1384904722469713 1105 26-27 0.09774216599051044 1105 28-29 0.03268653508685304 1105 30-31 0.07535208057542775 1105 32-33 0.14194651998099062 1105 34-35 0.19236636147781638 1105 36-37 0.2197335158754612 1105 38-39 0.17296885616809732 1105 40-41 0.2220738635915751 1105 42-43 0.1647185397518811 1105 44-45 0.23784661973939336 1105 46-47 0.2941589299255867 1105 48-49 0.17819277814193413 1105 50-51 0.26357072962004224 1105 52-53 0.2326176218812961 1105 54-55 0.24497081380142305 1105 56-57 0.28609693676720127 1105 58-59 0.2354206890927557 1105 60-61 0.21282526636365873 1105 62-63 0.297676091570132 1105 64-65 0.26323970509368166 1105 66-67 0.2611980040158812 1105 68-69 0.18837940067361814 1105 70-71 0.144261889968206 1105 72-73 0.16499174433487696 1105 74-75 0.09068842066101723 1105 76-77 0.08825388898099362 1105 78-79 0.06557131968491348 1105 80-81 0.10666934787736437 1105 82-83 0.09506864037054186 1105 84-85 0.10892326975147881 1105 86-87 0.09477138185083334 1105 88-89 0.18981150092881904 1105 90-91 0.18672160941989802 1105 92-93 0.18007472720538686 1105 94-95 0.14758637866010815 1105 96-97 0.13877783050391912 1105 98-99 0.15507917995327603 1105 100 0.1479693137469127 1106 1 0.10769471081658111 1106 2 0.13487259723767409 1106 3 0.08364385358044757 1106 4 0.015077464363137949 1106 5 0.060409471380225455 1106 6 0.0532145600360181 1106 7 0.07870412031088136 1106 8 0.00952121419665275 1106 9 0.03115365617374266 1106 10-11 0.10543354624363133 1106 12-13 0.10562875791545423 1106 14-15 0.09640736544788808 1106 16-17 0.08231494483113266 1106 18-19 0.10988924600264482 1106 20-21 0.14942060531842571 1106 22-23 0.1626700119147415 1106 24-25 0.19449316598185362 1106 26-27 0.13302279972708675 1106 28-29 0.16927838790205385 1106 30-31 0.14835147802946835 1106 32-33 0.19131848169069343 1106 34-35 0.16617265249389135 1106 36-37 0.18292197916514397 1106 38-39 0.2573927704266339 1106 40-41 0.21239120900855113 1106 42-43 0.1977222450005769 1106 44-45 0.19275143928915384 1106 46-47 0.18978794973871516 1106 48-49 0.18423187001519636 1106 50-51 0.14516124647361295 1106 52-53 0.17349245863496066 1106 54-55 0.1958121969852087 1106 56-57 0.19315081419135538 1106 58-59 0.11246177945556468 1106 60-61 0.08390974003567919 1106 62-63 0.14966040356352295 1106 64-65 0.1602997715645884 1106 66-67 0.151730943649504 1106 68-69 0.2030719113817625 1106 70-71 0.17959773436411552 1106 72-73 0.14151903475930538 1106 74-75 0.19549563321795915 1106 76-77 0.1516977526910992 1106 78-79 0.109804600397716 1106 80-81 0.11134350956144345 1106 82-83 0.0992537446709747 1106 84-85 0.08640924897046176 1106 86-87 0.14145846964532893 1106 88-89 0.09347230844946353 1106 90-91 0.1436480933597295 1106 92-93 0.11138005714700938 1106 94-95 0.16724887112828668 1106 96-97 0.17288915700476082 1106 98-99 0.14129151060650713 1106 100 0.16837544608345922 1107 1 0.09687467024443919 1107 2 0.1059170103380751 1107 3 0.034296256083415244 1107 4 0.022891366581994532 1107 5 0.047001139994954144 1107 6 0.044200960400935685 1107 7 0.07637630020114017 1107 8 0.044328198838954336 1107 9 0.06542787973027231 1107 10-11 0.01560026500443712 1107 12-13 0.07753666423005257 1107 14-15 0.08989340903360699 1107 16-17 0.14483097800678735 1107 18-19 0.15478159061545682 1107 20-21 0.12066693793470051 1107 22-23 0.1372322022500967 1107 24-25 0.1282646591925385 1107 26-27 0.1566966317458025 1107 28-29 0.19331348621989264 1107 30-31 0.2180899917092134 1107 32-33 0.17030790789343797 1107 34-35 0.2311570286293474 1107 36-37 0.24857121224308543 1107 38-39 0.2630134655463223 1107 40-41 0.22207885866976085 1107 42-43 0.267663882052382 1107 44-45 0.31344557401422435 1107 46-47 0.2945530970950969 1107 48-49 0.18569860462314125 1107 50-51 0.25093062877718353 1107 52-53 0.25280206680822914 1107 54-55 0.2964655187965448 1107 56-57 0.3761575303956164 1107 58-59 0.339060347260677 1107 60-61 0.3499413835051328 1107 62-63 0.3347191473680411 1107 64-65 0.4443301595214777 1107 66-67 0.3059221580383422 1107 68-69 0.37392657485798253 1107 70-71 0.3529678006331167 1107 72-73 0.29682173673791823 1107 74-75 0.37233346196920536 1107 76-77 0.40159181334619376 1107 78-79 0.36482489960194897 1107 80-81 0.3902131849055124 1107 82-83 0.3908546370129642 1107 84-85 0.40926388968286176 1107 86-87 0.38782873378450233 1107 88-89 0.314141993949697 1107 90-91 0.3265047653058559 1107 92-93 0.35523524681783414 1107 94-95 0.33087744253184326 1107 96-97 0.3126275305994284 1107 98-99 0.29960932513911587 1107 100 0.28507288769205275 1108 1 0.02026874302080728 1108 2 0.014455194980826036 1108 3 0.027041695160988866 1108 4 0.010252681082626225 1108 5 -0.028079015558098774 1108 6 -0.04565564228139607 1108 7 -0.03405152953787649 1108 8 -0.03963169564321589 1108 9 -0.02391193610110065 1108 10-11 -0.019897254250551555 1108 12-13 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-0.19774290224439994 2214 28-29 -0.1403989576296425 2214 30-31 -0.12437501237219095 2214 32-33 -0.15852584238908918 2214 34-35 -0.1761478397983467 2214 36-37 -0.17502941764371371 2214 38-39 -0.0810013069661153 2214 40-41 -0.06817520281941825 2214 42-43 -0.08366183894753476 2214 44-45 -0.027199524025348865 2214 46-47 0.02015921345043381 2214 48-49 -0.03309188483276415 2214 50-51 -0.021853378854494565 2214 52-53 -0.032492449255052236 2214 54-55 -0.03450258195725553 2214 56-57 -0.10097936575419908 2214 58-59 -0.009317699768672583 2214 60-61 -0.03078914214653139 2214 62-63 -0.04841729859861488 2214 64-65 -0.052834388888094 2214 66-67 -0.11015005496112451 2214 68-69 -0.09855311561707225 2214 70-71 -0.033340625461164564 2214 72-73 0.004626168011157006 2214 74-75 -0.009415704702234962 2214 76-77 -0.09579953607779856 2214 78-79 -0.06292380025396227 2214 80-81 -0.06249619322634459 2214 82-83 -0.14797809499815173 2214 84-85 -0.10169065937536459 2214 86-87 -0.12133349126604287 2214 88-89 -0.10138841919314245 2214 90-91 -0.045562691571461755 2214 92-93 0.005994163946255071 2214 94-95 -0.0443655057538308 2214 96-97 -0.05663778565331512 2214 98-99 -0.03630963524089026 2214 100 -0.022980918434612363 2215 1 -0.1892253008941438 2215 2 -0.2878835358087031 2215 3 -0.25668686271824726 2215 4 -0.1715259330886596 2215 5 -0.18891727355164534 2215 6 -0.15683208173417995 2215 7 -0.2432363963467381 2215 8 -0.13163271705422375 2215 9 -0.2232099306163846 2215 10-11 -0.24050558032385538 2215 12-13 -0.28868639920719374 2215 14-15 -0.3682811046260639 2215 16-17 -0.2862936017648181 2215 18-19 -0.3040857971862465 2215 20-21 -0.2989966027458877 2215 22-23 -0.3585917183675491 2215 24-25 -0.36958674855665663 2215 26-27 -0.3323118275968966 2215 28-29 -0.338100599388234 2215 30-31 -0.3537878528052829 2215 32-33 -0.3538985578642979 2215 34-35 -0.3379545966250177 2215 36-37 -0.3368580312493634 2215 38-39 -0.3389782450766745 2215 40-41 -0.2874710670293794 2215 42-43 -0.2951976817850337 2215 44-45 -0.3236008368158281 2215 46-47 -0.28839663054122155 2215 48-49 -0.27805020206834286 2215 50-51 -0.24408941225479452 2215 52-53 -0.29816489979150873 2215 54-55 -0.30197974987848397 2215 56-57 -0.3062826458591985 2215 58-59 -0.29038946972591617 2215 60-61 -0.321420540463059 2215 62-63 -0.223520131598832 2215 64-65 -0.33915827207351157 2215 66-67 -0.24573649985852342 2215 68-69 -0.31057393522112164 2215 70-71 -0.33592261078944574 2215 72-73 -0.3362300249184713 2215 74-75 -0.2966155494090401 2215 76-77 -0.27490738796054615 2215 78-79 -0.33035615391614215 2215 80-81 -0.2935663357183955 2215 82-83 -0.2890417858093244 2215 84-85 -0.29730395666231146 2215 86-87 -0.4484113043868092 2215 88-89 -0.360969275359345 2215 90-91 -0.3151662960845272 2215 92-93 -0.2935934819732964 2215 94-95 -0.3332282739573458 2215 96-97 -0.33362214138858803 2215 98-99 -0.42852587803542974 2215 100 -0.47997644785847626 2216 1 -0.18640184458291742 2216 2 -0.20706772551248775 2216 3 -0.23461994971627576 2216 4 -0.1455982811778398 2216 5 -0.1503723288314589 2216 6 -0.126756182038811 2216 7 -0.09596521523909018 2216 8 -0.12164978223952261 2216 9 -0.14087758271254813 2216 10-11 -0.2095737735457064 2216 12-13 -0.24705796040907302 2216 14-15 -0.3007968359731876 2216 16-17 -0.32333116591372146 2216 18-19 -0.27311846552015595 2216 20-21 -0.2606689997314362 2216 22-23 -0.27130949585994557 2216 24-25 -0.33607133362376373 2216 26-27 -0.31369866185277573 2216 28-29 -0.33540622419157273 2216 30-31 -0.268562543374685 2216 32-33 -0.3918128806606447 2216 34-35 -0.3982162804517486 2216 36-37 -0.4120026572605511 2216 38-39 -0.29313580435358944 2216 40-41 -0.3048221063199037 2216 42-43 -0.3302381294074479 2216 44-45 -0.3681385653500797 2216 46-47 -0.30611251705045817 2216 48-49 -0.42563636316069164 2216 50-51 -0.4854814671246359 2216 52-53 -0.43851093426521004 2216 54-55 -0.37932923578260613 2216 56-57 -0.4407113593242755 2216 58-59 -0.393529141670129 2216 60-61 -0.4141844076361636 2216 62-63 -0.3816519991002636 2216 64-65 -0.44351384398670035 2216 66-67 -0.5350131248794341 2216 68-69 -0.578322367823354 2216 70-71 -0.61741067994339 2216 72-73 -0.587256495593671 2216 74-75 -0.5672504343194333 2216 76-77 -0.565958492166736 2216 78-79 -0.5874692956092531 2216 80-81 -0.5717203610412369 2216 82-83 -0.5400013990867194 2216 84-85 -0.5459020474974636 2216 86-87 -0.480088251205828 2216 88-89 -0.48019455036831005 2216 90-91 -0.48891984691843504 2216 92-93 -0.5620696231496467 2216 94-95 -0.5857379638669897 2216 96-97 -0.4730103277967217 2216 98-99 -0.3705923982777648 2216 100 -0.4047550351329754 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 1.0 4 6.0 5 20.0 6 49.0 7 130.0 8 277.0 9 479.0 10 789.0 11 1232.0 12 2056.0 13 3103.0 14 3638.0 15 4770.0 16 6146.0 17 9453.0 18 11575.0 19 6442.0 20 4341.0 21 4146.0 22 4373.0 23 4443.0 24 5121.0 25 5994.0 26 7455.0 27 9554.0 28 12013.0 29 15568.0 30 19761.0 31 24770.0 32 31613.0 33 41955.0 34 59891.0 35 96423.0 36 190389.0 37 421868.0 38 689958.0 39 313498.0 40 1024.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.168564739336865 18.981752687253888 13.209890762359978 23.639791811049264 2 16.103316358191705 19.55763687759331 39.65282542279819 24.686221341416797 3 18.822989747428913 24.93312893059905 29.473709294036116 26.770172027935924 4 12.699612182754223 16.156056348937213 37.45919379095206 33.68513767735651 5 13.092451501026897 37.610241863138995 34.93306181090303 14.36424482493108 6 31.84447847963639 37.03577749589799 17.022224097743337 14.097519926722288 7 28.505479273878304 32.25146178508061 21.369267414579028 17.873791526462057 8 26.38985002326608 35.55231089846841 19.303737685880073 18.75410139238543 9 26.061816793567218 16.060262909278478 20.279877893488926 37.598042403665374 10-11 24.9715786390512 26.14415214984635 27.620871456017632 21.263397755084817 12-13 25.731583932706094 23.497811677144934 28.219535249472777 22.551069140676198 14-15 22.72958796143226 25.124781192319418 25.7504247078179 26.39520613843042 16-17 22.09898576697959 28.72049267001931 26.267716809063064 22.912804753938033 18-19 21.97668103375521 27.081167596506226 29.06461019715776 21.877541172580802 20-21 22.824437902429096 26.3934161731196 28.996018527252236 21.786127397199067 22-23 22.68210309552674 26.101112729518462 28.961871117187588 22.254913057767215 24-25 22.335031176774294 26.188738631399183 29.029548433218157 22.446681758608364 26-27 22.144780796247463 26.334064885472497 29.08231289629361 22.43884142198643 28-29 22.27306724840835 26.367236517662384 29.112371452125842 22.247324781803428 30-31 22.319729340167356 26.573465982072857 28.789334477302102 22.317470200457688 32-33 22.241197906037844 26.383565684085404 29.088978495824936 22.28625791405182 34-35 22.39709169565594 26.192168662583715 29.041654428313958 22.36908521344639 36-37 22.516350734966746 26.456294949156845 28.671313911514957 22.356040404361455 38-39 22.39415067998544 26.737038359591935 28.63569835395995 22.23311260646268 40-41 22.311250408226364 26.453242403885195 28.81339305338421 22.422114134504223 42-43 22.32195152485234 26.51627040724827 28.56997370524539 22.591804362653995 44-45 22.36575080329483 26.646552510297905 28.514425761294397 22.473270925112868 46-47 22.546083582025755 26.723569580573987 28.17862216818044 22.551724669219816 48-49 22.63214251190983 26.83949120781115 27.87830715047002 22.650059129809 50-51 22.58475504991643 26.912253741557794 27.7923454683079 22.710645740217874 52-53 22.65723735379554 26.89940934267319 27.733883652682557 22.709469650848714 54-55 22.576838231731 27.137319396203495 27.617753265287813 22.66808910677769 56-57 22.441705636415904 26.919874584534558 27.834898624817782 22.803521154231753 58-59 22.513855152138973 26.468750473598128 28.11936884058457 22.89802553367832 60-61 22.641338331488175 26.839276592615263 27.833574164362705 22.685810911533853 62-63 22.33437954425353 26.85286289369866 27.94886262140059 22.86389494064722 64-65 22.467445751192706 26.617361604106577 27.956417270258598 22.958775374442123 66-67 22.34148443476711 26.73937574249291 27.843018692577033 23.076121130162946 68-69 22.587116543858933 26.85032597728026 27.792898622493116 22.7696588563677 70-71 22.565374731393252 26.647427841373517 27.819182512096457 22.968014915136774 72-73 22.404372669820734 26.788957122219298 27.809867300789843 22.996802907170128 74-75 22.468488281463316 26.973137470027254 27.62255936217005 22.93581488633938 76-77 22.565546069345725 26.880236704309503 27.476465470485806 23.077751755858966 78-79 22.494725293273692 26.942978558627434 27.543922793186816 23.018373354912054 80-81 22.494036463827833 26.869000781899643 27.611308999292568 23.025653754979956 82-83 22.4547317564287 26.880008499120798 27.569867042375463 23.09539270207504 84-85 22.480576668172546 26.9021461230285 27.469605278181824 23.14767193061713 86-87 22.53481585772214 26.894269606694493 27.488566815865667 23.0823477197177 88-89 22.226988094998738 26.93231939390013 27.507525486869895 23.333167024231237 90-91 22.266557382908843 26.841060641563836 27.48199399501968 23.410387980507643 92-93 22.41537017643454 26.922705543046487 27.385269395350733 23.276654885168245 94-95 22.2271646259807 26.753816248654317 27.46872447177439 23.55029465359059 96-97 22.293779086955368 26.675690361021413 27.54267989821908 23.487850653804138 98-99 22.161195574011103 26.88534820147349 27.449580617272396 23.503875607243003 100 22.252560404700322 26.62979750090601 27.41219164685032 23.705450447543353 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 107.0 1 97.0 2 269.0 3 482.5 4 314.5 5 175.0 6 502.0 7 1155.0 8 2002.5 9 2311.0 10 1686.5 11 1085.0 12 1012.0 13 1154.0 14 1307.0 15 1435.5 16 1592.5 17 1745.0 18 1972.0 19 2199.0 20 2414.5 21 2794.0 22 3463.0 23 4511.0 24 5894.0 25 7800.0 26 10296.5 27 13017.5 28 16517.5 29 21089.5 30 25516.0 31 29590.5 32 33745.5 33 38264.0 34 42637.5 35 46617.5 36 51173.5 37 55464.5 38 59064.5 39 61255.5 40 63438.0 41 66782.5 42 68850.0 43 69948.0 44 70281.0 45 71026.0 46 72027.0 47 70867.5 48 69912.5 49 68707.0 50 66924.0 51 65327.0 52 61469.0 53 58551.5 54 57188.0 55 54593.0 56 51150.0 57 46994.0 58 43242.0 59 40717.0 60 37874.5 61 33534.5 62 29195.5 63 25334.5 64 21088.5 65 17202.5 66 14023.0 67 11063.5 68 8540.5 69 6593.0 70 4781.0 71 3511.0 72 2804.0 73 2113.5 74 1561.0 75 1154.0 76 783.5 77 572.0 78 403.5 79 233.5 80 141.0 81 61.0 82 31.5 83 21.5 84 14.0 85 8.5 86 6.0 87 4.0 88 1.0 89 0.5 90 0.5 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.9786579160316336E-4 2 5.957315832063267E-4 3 2.9786579160316336E-4 4 0.011765698768324952 5 4.46798687404745E-4 6 0.003723322395039542 7 5.957315832063267E-4 8 0.13989763345628572 9 0.0011418188678121262 10-11 0.0 12-13 9.928859720105445E-5 14-15 1.985771944021089E-4 16-17 0.0 18-19 1.985771944021089E-4 20-21 0.0 22-23 1.985771944021089E-4 24-25 4.964429860052723E-4 26-27 0.005932493682763003 28-29 0.007372178342178293 30-31 0.014347202295552367 32-33 0.017325860211584002 34-35 0.02534341443556915 36-37 0.03651338162068777 38-39 0.04125441213703812 40-41 0.052002402784052264 42-43 0.0685587763673281 44-45 0.10666077554323274 46-47 0.11450457472211603 48-49 0.1107812523270765 50-51 0.11254362492739522 52-53 0.10747990647014144 54-55 0.11390884313890971 56-57 0.09822124478114311 58-59 0.08993064691485506 60-61 0.09204052960537747 62-63 0.09082424428966455 64-65 0.0 66-67 4.9644298600527226E-5 68-69 1.2411074650131807E-4 70-71 2.4822149300263613E-5 72-73 0.0 74-75 0.0 76-77 1.2411074650131807E-4 78-79 0.0 80-81 2.4822149300263613E-4 82-83 1.985771944021089E-4 84-85 0.0 86-87 8.9359737480949E-4 88-89 4.2197653810448145E-4 90-91 9.928859720105445E-5 92-93 6.701980311071175E-4 94-95 7.69486628308172E-4 96-97 1.737550451018453E-4 98-99 2.4822149300263613E-5 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2014330.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.729146361238264 #Duplication Level Percentage of deduplicated Percentage of total 1 77.82194599797704 48.03882294625505 2 13.331850763883493 16.459275341399007 3 3.8791450334584345 7.183689345804786 4 1.661339711933392 4.1021232893469515 5 0.8547241223007783 2.638069522199283 6 0.5319872880683931 1.9703472700495237 7 0.36794653564996005 1.589911788657287 8 0.2757197756658739 1.3615957109413208 9 0.18589494302588797 1.0327622531273177 >10 1.0223370181240965 11.18699923259271 >50 0.052857417130807 2.2213315404638663 >100 0.013607713843436557 1.3934274473527126 >500 3.2183946923365305E-4 0.13335640314395192 >1k 2.4137960192523904E-4 0.41308201301610625 >5k 8.045986730841302E-5 0.27520589565014364 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5541 0.27507905854552134 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4530 0.2248886726603883 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2450 0.12162853157129169 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 2.482214930026361E-5 0.0 0.0 0.0 16-17 0.0 4.964429860052722E-5 0.0 0.0 0.0 18-19 0.0 1.4893289580158168E-4 0.0 0.0 0.0 20-21 0.0 1.4893289580158168E-4 0.0 1.4893289580158168E-4 0.0 22-23 0.0 1.4893289580158168E-4 0.0 1.4893289580158168E-4 0.0 24-25 0.0 1.985771944021089E-4 0.0 2.730436423028997E-4 0.0 26-27 0.0 2.4822149300263613E-4 0.0 4.964429860052723E-4 0.0 28-29 0.0 2.4822149300263613E-4 0.0 0.001861661197519771 0.0 30-31 0.0 2.4822149300263613E-4 0.0 0.005386406398157203 0.0 32-33 0.0 2.4822149300263613E-4 0.0 0.013652182115144986 0.0 34-35 0.0 2.9786579160316336E-4 0.0 0.024995904345365458 0.0 36-37 0.0 3.2268794090342694E-4 0.0 0.0463181305942919 0.0 38-39 0.0 3.4751009020369053E-4 0.0 0.08374993173908943 0.0 40-41 0.0 3.4751009020369053E-4 0.0 0.13123470335049373 0.0 42-43 0.0 3.4751009020369053E-4 0.0 0.1774287231982843 0.0 44-45 0.0 3.4751009020369053E-4 0.0 0.23223602885326633 0.0 46-47 0.0 3.9715438880421776E-4 0.0 0.2971459492734557 0.0 48-49 0.0 3.9715438880421776E-4 0.0 0.37106630988964073 0.0 50-51 0.0 3.9715438880421776E-4 0.0 0.4538978221046204 0.0 52-53 0.0 3.9715438880421776E-4 0.0 0.5385413512185193 0.0 54-55 0.0 3.9715438880421776E-4 0.0 0.6288691525221786 0.0 56-57 0.0 3.9715438880421776E-4 0.0 0.7320548271633744 0.0 58-59 0.0 4.46798687404745E-4 0.0 0.8588711879384212 0.0 60-61 0.0 4.46798687404745E-4 0.0 0.9967085830027851 0.0 62-63 0.0 4.46798687404745E-4 0.0 1.1345708002164492 0.0 64-65 0.0 4.46798687404745E-4 0.0 1.2770002929013617 0.0 66-67 0.0 4.716208367050086E-4 0.0 1.4407023675366002 0.0 68-69 0.0 4.964429860052723E-4 0.0 1.630219477444113 0.0 70-71 0.0 4.964429860052723E-4 0.0 1.838601420819826 0.0 72-73 0.0 4.964429860052723E-4 0.0 2.0559441600929342 0.0 74-75 0.0 4.964429860052723E-4 0.0 2.2679253151171856 0.0 76-77 0.0 4.964429860052723E-4 0.0 2.4903565949968476 0.0 78-79 0.0 4.964429860052723E-4 0.0 2.756698256988676 0.0 80-81 0.0 5.460872846057994E-4 0.0 3.04706775950316 0.0 82-83 0.0 5.957315832063267E-4 0.0 3.3521319744033997 0.0 84-85 0.0 5.957315832063267E-4 0.0 3.650221165350265 0.0 86-87 0.0 5.957315832063267E-4 0.0 3.953374074754385 0.0 88 0.0 5.957315832063267E-4 0.0 4.202538809430431 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3660 0.0 48.907745 1 GTATCAA 6315 0.0 40.100445 1 TCAACGC 7430 0.0 33.49526 4 ATCAACG 7460 0.0 33.424454 3 CAACGCA 7645 0.0 32.60914 5 AACGCAG 7725 0.0 32.332325 6 TATCAAC 7820 0.0 32.066216 2 GTACATG 7665 0.0 27.889006 1 TACATGG 7610 0.0 27.263475 2 ACGCAGA 9205 0.0 26.978552 7 CGCAGAG 9435 0.0 26.419931 8 ACATGGG 7855 0.0 25.6345 3 GCAGAGT 10385 0.0 23.92509 9 TATACCG 145 2.5190726E-5 22.707584 5 GAGTACT 6410 0.0 21.176014 12-13 CATGGGG 5550 0.0 20.513458 4 CAGAGTA 10160 0.0 20.363695 10-11 GTGGTAT 1880 0.0 19.74254 1 AGAGTAC 9590 0.0 19.541536 10-11 GAGTACA 5655 0.0 19.357847 1 >>END_MODULE