FastQCFastQC Report
Wed 25 May 2016
SRR1032928_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032928_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2547416
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG97300.028.4031351
TACATGG96500.027.4696772
GTATCAA49850.027.2479881
GGTATCA34850.025.75921
ACATGGG97800.025.6147483
TCAACGC52300.024.6235494
ATCAACG52900.024.2554153
CAACGCA53350.024.1389245
AACGCAG55950.023.101196
TATCAAC61200.021.3498652
CATGGGG67900.021.1121184
GAGTACA68050.019.8223671
ACGCAGA66300.019.2113347
CGCAGAG68150.018.3449928
TATATCG2352.7986516E-618.0001625
AGTACAT70950.017.952242
ACACCGT5100.017.5099626
GTACCGT2152.62417E-517.488536
GTATAGG10750.017.488531
TACCGCG2756.6054236E-717.0910638
Produced by FastQC (version 0.11.5)