FastQCFastQC Report
Wed 25 May 2016
SRR1032928_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032928_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2547416
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70640.27730060579033816No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59280.23270639738464388No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31320.12294811683682602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57750.042.300011
GTATCAA90800.035.0261151
TCAACGC107150.029.2388174
CAACGCA108250.028.9851575
ATCAACG109500.028.6972353
TATCAAC111900.028.4595342
GTACATG94000.028.2364251
AACGCAG111650.028.1451786
TACATGG94850.027.276872
ACATGGG95500.025.9588223
ACGCAGA128400.024.1795447
CGCAGAG132950.023.3166588
CCGATCG1903.6595884E-722.2528979
CATGGGG66200.022.0993824
GCAGAGT141900.021.6848569
GAGTACT86000.020.92113312-13
TATAACG2151.1854063E-619.6912482
AGAGTAC121150.019.62077710-11
CTAGCGT1200.005040232619.600474
GTACTTT92700.019.38350514-15
Produced by FastQC (version 0.11.5)