FastQCFastQC Report
Wed 25 May 2016
SRR1032927_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032927_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2868047
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39850.038.568011
GGTATCA23900.036.578241
TCAACGC40650.035.7277534
ATCAACG40850.035.667893
CAACGCA43100.033.6968235
AACGCAG44600.032.4581386
TATCAAC48500.030.526472
GTACATG105300.029.191551
TACATGG104000.028.2458382
ACATGGG104100.027.1351073
ACGCAGA57250.025.204077
CGCAGAG60650.023.5586578
TAGACCG1000.00175908423.5005365
ATACCGT3500.021.4862066
CATGGGG70000.021.1504824
GCAGAGT70700.019.7444389
TATACCG2401.5815385E-719.5837785
GAGTACA76100.019.3933471
CATGGGA36450.019.0841124
ACACCGT5750.017.9830196
Produced by FastQC (version 0.11.5)