FastQCFastQC Report
Wed 25 May 2016
SRR1032927_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032927_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2868047
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70220.24483559718512285No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57020.19881124681708495No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31740.11066764247587295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43150.053.7870331
GTATCAA83500.043.4372441
ATCAACG98600.036.1747783
TCAACGC98650.036.0610474
CAACGCA101050.035.2909245
AACGCAG103200.034.600686
TATCAAC104150.034.5175632
GTACATG97050.029.9174351
ACGCAGA121250.029.3696547
TACATGG96500.028.915682
CGCAGAG124600.028.504518
ACATGGG97950.027.1910273
GCAGAGT139350.025.4815069
GTGGTAT20900.023.3786011
CATGGGG64450.022.9255814
GAGTACT91700.021.64014212-13
TGGTATC21100.021.6319312
CAGAGTA134700.021.11296810-11
AGAGTAC127550.020.36326210-11
GTACTTT99300.019.70010414-15
Produced by FastQC (version 0.11.5)