FastQCFastQC Report
Wed 25 May 2016
SRR1032926_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032926_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1963452
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG67200.029.5161461
TACATGG67350.028.1244432
ACATGGG69750.026.1452563
GTATCAA37350.025.4200971
TCAACGC38000.023.9951534
CAACGCA39500.023.321335
ATCAACG39400.023.1425323
AACGCAG40600.022.573716
ACACCGT3550.022.5068596
TACCTGG14000.021.8223652
TATCAAC42650.021.8203952
CATGGGG47300.021.562744
GGTATCA26950.021.1029431
TAACCCG1200.005065622319.5831745
ACGCAGA47550.019.2742927
GAGTACA47450.019.2168431
GTACCTG17300.019.0181561
CGCGCGT1508.2937256E-418.7998497
CGCAGAG50550.018.037448
AGTACAT48050.017.998692
Produced by FastQC (version 0.11.5)