##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032926_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1963452 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.554239675836232 33.0 31.0 34.0 30.0 34.0 2 31.666725236980582 34.0 31.0 34.0 30.0 34.0 3 31.742600786777572 34.0 31.0 34.0 30.0 34.0 4 35.077673403780686 37.0 35.0 37.0 33.0 37.0 5 35.15366813143382 37.0 35.0 37.0 33.0 37.0 6 35.271685786054356 37.0 36.0 37.0 33.0 37.0 7 35.23375106699833 37.0 36.0 37.0 33.0 37.0 8 35.24816292937133 37.0 37.0 37.0 33.0 37.0 9 36.904191699109525 39.0 38.0 39.0 34.0 39.0 10-11 36.89267371955108 39.0 38.0 39.0 34.0 39.0 12-13 36.83615973296011 39.0 38.0 39.0 34.0 39.0 14-15 38.32356202239728 41.0 39.0 41.0 34.0 41.0 16-17 38.34332721146227 41.0 39.0 41.0 34.0 41.0 18-19 38.35438324950139 41.0 39.0 41.0 34.0 41.0 20-21 38.389241499155574 41.0 39.0 41.0 34.0 41.0 22-23 38.38421871275692 41.0 39.0 41.0 34.0 41.0 24-25 38.41740465262201 41.0 39.0 41.0 34.0 41.0 26-27 38.39603285438096 41.0 39.0 41.0 34.0 41.0 28-29 38.379579943894726 41.0 39.0 41.0 34.0 41.0 30-31 38.33976537241552 41.0 39.0 41.0 34.0 41.0 32-33 38.29053065723022 41.0 39.0 41.0 34.0 41.0 34-35 38.22288983891636 41.0 39.0 41.0 33.5 41.0 36-37 38.137585487192965 41.0 39.0 41.0 33.0 41.0 38-39 38.047706539299156 41.0 38.0 41.0 33.0 41.0 40-41 37.963898786423094 41.0 38.0 41.0 33.0 41.0 42-43 37.85189120997101 40.5 38.0 41.0 33.0 41.0 44-45 37.72601443783704 40.0 38.0 41.0 33.0 41.0 46-47 37.605243214501805 40.0 38.0 41.0 32.0 41.0 48-49 37.47137617828193 40.0 38.0 41.0 32.0 41.0 50-51 37.330229870656375 40.0 37.0 41.0 32.0 41.0 52-53 37.17445040673263 40.0 37.0 41.0 31.0 41.0 54-55 37.00388372111974 40.0 36.5 41.0 31.0 41.0 56-57 36.8138915542626 40.0 36.0 41.0 31.0 41.0 58-59 36.60501504493107 39.5 35.5 41.0 31.0 41.0 60-61 36.358279193990995 39.0 35.0 41.0 30.5 41.0 62-63 36.09674033284236 39.0 35.0 41.0 30.0 41.0 64-65 35.81189099606203 38.5 35.0 40.5 30.0 41.0 66-67 35.50434438937137 37.5 35.0 40.0 30.0 41.0 68-69 35.18076454122637 37.0 35.0 40.0 29.0 41.0 70-71 34.832558167961324 36.5 34.5 39.5 29.0 41.0 72-73 34.53632378076979 36.0 34.0 39.0 29.0 41.0 74-75 34.25738113282117 36.0 34.0 39.0 29.0 41.0 76-77 33.93471167107727 35.0 34.0 37.5 29.0 40.0 78-79 33.5766789817118 35.0 34.0 37.0 28.0 39.0 80-81 33.2653596828443 35.0 34.0 37.0 28.0 39.0 82-83 32.964127465300905 35.0 34.0 36.0 27.5 38.5 84-85 32.80612436667665 35.0 34.0 36.0 28.0 37.0 86-87 32.59061311404608 35.0 34.0 36.0 27.5 37.0 88-89 32.372655914175645 35.0 34.0 35.0 27.0 37.0 90-91 32.18828013111602 35.0 34.0 35.0 27.0 36.0 92-93 32.004526721305126 35.0 34.0 35.0 27.0 36.0 94-95 31.83576578393564 35.0 33.0 35.0 26.0 36.0 96-97 31.671547356390683 35.0 33.0 35.0 25.5 36.0 98-99 31.502209883409424 35.0 33.0 35.0 25.0 35.0 100 31.38053489466511 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.28587905257684554 1101 2 0.3473178795041143 1101 3 0.3152149554603767 1101 4 0.34408510028074346 1101 5 0.1909869380285798 1101 6 0.26472749552258534 1101 7 0.2680390025656436 1101 8 0.23523260222087572 1101 9 0.23324221609329499 1101 10-11 0.22615230555148713 1101 12-13 0.04608145809824293 1101 14-15 0.059074321537899266 1101 16-17 0.0400808549357663 1101 18-19 0.06866197741345559 1101 20-21 0.05326218138778671 1101 22-23 0.12549769650559028 1101 24-25 0.1576489854468477 1101 26-27 0.15393287694344338 1101 28-29 0.14498919296746493 1101 30-31 0.2028316948073865 1101 32-33 0.1701293100156036 1101 34-35 0.21651032572277984 1101 36-37 0.20615764626128907 1101 38-39 0.18282245621718118 1101 40-41 0.21196139360881006 1101 42-43 0.2478085661562659 1101 44-45 0.18697354802699806 1101 46-47 0.1496071641647987 1101 48-49 0.14548918779921394 1101 50-51 0.15502199634249791 1101 52-53 0.1668093434731759 1101 54-55 0.27216100810423427 1101 56-57 0.18943301573575155 1101 58-59 0.17936440405824783 1101 60-61 0.11919367835047012 1101 62-63 0.16374945466586865 1101 64-65 0.18572055182397662 1101 66-67 0.18032923508750542 1101 68-69 0.19436326364308343 1101 70-71 0.28087372982233205 1101 72-73 0.1010212983005303 1101 74-75 0.118270637853648 1101 76-77 0.1664633223798333 1101 78-79 0.13004795109361567 1101 80-81 0.18086176477612526 1101 82-83 0.0020262023211614633 1101 84-85 0.044190979532508834 1101 86-87 0.06334848588443975 1101 88-89 0.028732607200929294 1101 90-91 0.036259057072413725 1101 92-93 0.06007940006558954 1101 94-95 0.10099442873550046 1101 96-97 0.06434385390149089 1101 98-99 0.1220176416220653 1101 100 0.2202552692884403 1102 1 0.2293069049286416 1102 2 0.21312475232150874 1102 3 0.2922225631430422 1102 4 0.3224235093018919 1102 5 0.29697559975715393 1102 6 0.3205362010467212 1102 7 0.3464891565631447 1102 8 0.25899512130693125 1102 9 0.4042861180540669 1102 10-11 0.36974111857585257 1102 12-13 0.3751594116388475 1102 14-15 0.4345318715376507 1102 16-17 0.38882753976980666 1102 18-19 0.4321015111817701 1102 20-21 0.37961544591755825 1102 22-23 0.37861691881403914 1102 24-25 0.4357349377218256 1102 26-27 0.48190828190757173 1102 28-29 0.45322205702017726 1102 30-31 0.4422224674353217 1102 32-33 0.4854169809127242 1102 34-35 0.5111218336727745 1102 36-37 0.4804237419107551 1102 38-39 0.4550931854394946 1102 40-41 0.4597076935187303 1102 42-43 0.4490216620435916 1102 44-45 0.5095722704799925 1102 46-47 0.5161336111257597 1102 48-49 0.5178333645545834 1102 50-51 0.583000938597749 1102 52-53 0.5037974598461545 1102 54-55 0.5859105144153816 1102 56-57 0.552574665026988 1102 58-59 0.4866473522539181 1102 60-61 0.5229010936824778 1102 62-63 0.5172948787759353 1102 64-65 0.520461792014963 1102 66-67 0.5117579553289957 1102 68-69 0.5487200364971869 1102 70-71 0.5682145726111898 1102 72-73 0.4365763939772975 1102 74-75 0.44517594262052285 1102 76-77 0.4364601583147021 1102 78-79 0.5114693182259913 1102 80-81 0.5214726800209561 1102 82-83 0.4357134133147298 1102 84-85 0.3996813567080082 1102 86-87 0.41126460362108475 1102 88-89 0.39388382778918896 1102 90-91 0.43172152028597566 1102 92-93 0.447608582717379 1102 94-95 0.41551796119140505 1102 96-97 0.4984874311556524 1102 98-99 0.4374417906807295 1102 100 0.29149881930267085 1103 1 0.1229619326682645 1103 2 0.21657516708473779 1103 3 0.26570061104047227 1103 4 0.15249836900650138 1103 5 0.09627997952905787 1103 6 0.15150793964472342 1103 7 0.13172965534388226 1103 8 0.23071721397110423 1103 9 0.26511308884493445 1103 10-11 0.3450038729444387 1103 12-13 0.3235940664642598 1103 14-15 0.2857282424925103 1103 16-17 0.3015896286004818 1103 18-19 0.2231368485828753 1103 20-21 0.30135870268740916 1103 22-23 0.2786716260306008 1103 24-25 0.25211023188958137 1103 26-27 0.2857538930843475 1103 28-29 0.31549990046436704 1103 30-31 0.20922336724258628 1103 32-33 0.2642286496558697 1103 34-35 0.21731160249152026 1103 36-37 0.22391291760212084 1103 38-39 0.24329412997090571 1103 40-41 0.24757467112471687 1103 42-43 0.17353169303103755 1103 44-45 0.21645885749507698 1103 46-47 0.17564481345993244 1103 48-49 0.2157166877717458 1103 50-51 0.2598096044338263 1103 52-53 0.20724827393212308 1103 54-55 0.29553139739783774 1103 56-57 0.28265045648467435 1103 58-59 0.2956978692437744 1103 60-61 0.1957498089280847 1103 62-63 0.24522710571904582 1103 64-65 0.1208706985095489 1103 66-67 0.17081912092547924 1103 68-69 0.14532605934910237 1103 70-71 0.23041348325873656 1103 72-73 0.18053078067079298 1103 74-75 0.1785364299007668 1103 76-77 0.19947155764810276 1103 78-79 0.12469914710288776 1103 80-81 0.1686655742057539 1103 82-83 0.15716157057335067 1103 84-85 0.15114261175278187 1103 86-87 0.08526177109173005 1103 88-89 0.061008650756100735 1103 90-91 0.0670939286813308 1103 92-93 0.0952512474246987 1103 94-95 0.06466434226243933 1103 96-97 0.10667967045113613 1103 98-99 0.1868438714184677 1103 100 0.23830583460472 1104 1 0.06884079464325055 1104 2 0.2210828209906488 1104 3 0.1534225233707751 1104 4 0.198510951467469 1104 5 0.16419279377220164 1104 6 0.17576037845168457 1104 7 0.12281368275021265 1104 8 0.18697787977177427 1104 9 0.20842579101757508 1104 10-11 0.21973834226513844 1104 12-13 0.22702877266125654 1104 14-15 0.27213842053178183 1104 16-17 0.24452982370503662 1104 18-19 0.27893044782894805 1104 20-21 0.22993827274420653 1104 22-23 0.2928947100070616 1104 24-25 0.31022929406324096 1104 26-27 0.36694674349569567 1104 28-29 0.2849173885568703 1104 30-31 0.2445577321339556 1104 32-33 0.28434848868187146 1104 34-35 0.2859974484964667 1104 36-37 0.3538554814695658 1104 38-39 0.3702671439033054 1104 40-41 0.3163452067707766 1104 42-43 0.27736469524697327 1104 44-45 0.23233943873345453 1104 46-47 0.20857985077432062 1104 48-49 0.29600310431742827 1104 50-51 0.2628132212545893 1104 52-53 0.29423758902853336 1104 54-55 0.2950872543551242 1104 56-57 0.3002356555371719 1104 58-59 0.32003436092763593 1104 60-61 0.3521276393450421 1104 62-63 0.3034518707530438 1104 64-65 0.29755748692552686 1104 66-67 0.3510520492767668 1104 68-69 0.36512751626563045 1104 70-71 0.4136477997560135 1104 72-73 0.4030146068596352 1104 74-75 0.3749578149902959 1104 76-77 0.38014353492835085 1104 78-79 0.35544085006510784 1104 80-81 0.32620900152548415 1104 82-83 0.27959668429944173 1104 84-85 0.29286021955697095 1104 86-87 0.32111159661833 1104 88-89 0.2611073492100431 1104 90-91 0.2302205046727721 1104 92-93 0.22909717595427992 1104 94-95 0.32820608852463096 1104 96-97 0.2688565126100215 1104 98-99 0.25009111422939867 1104 100 0.11590956349598613 1105 1 0.03588983071646723 1105 2 0.06551132660553094 1105 3 0.04252828596861136 1105 4 0.08389397494832451 1105 5 0.05178411026535201 1105 6 0.14716664635239596 1105 7 0.1586719054393626 1105 8 0.09413255900316386 1105 9 0.17867617554014714 1105 10-11 0.18187597087605667 1105 12-13 0.23092536960603383 1105 14-15 0.20950560485155023 1105 16-17 0.2303505082161479 1105 18-19 0.2591483764527851 1105 20-21 0.19165323122064137 1105 22-23 0.21339920849456462 1105 24-25 0.21062472464320336 1105 26-27 0.17562177987603889 1105 28-29 0.25739270232973155 1105 30-31 0.11950221706870678 1105 32-33 0.18069832743833558 1105 34-35 0.2368004364379317 1105 36-37 0.23625336665637064 1105 38-39 0.21864715214221064 1105 40-41 0.28329046440699557 1105 42-43 0.22571088256248117 1105 44-45 0.1945687189998324 1105 46-47 0.19596105097225092 1105 48-49 0.17994913465509654 1105 50-51 0.1381755012357715 1105 52-53 0.17721274557980138 1105 54-55 0.2064182996471331 1105 56-57 0.1334426834641178 1105 58-59 0.04797178429734572 1105 60-61 0.12772733611456033 1105 62-63 0.16416743617794083 1105 64-65 0.2089594038101552 1105 66-67 0.1970126122519389 1105 68-69 0.24118376503162864 1105 70-71 0.2081278594270941 1105 72-73 0.19380215295968384 1105 74-75 0.12949725323390027 1105 76-77 0.15848053270287465 1105 78-79 0.17409360729303813 1105 80-81 0.2180462150226603 1105 82-83 0.20604245260113174 1105 84-85 0.1789476932619678 1105 86-87 0.05281338016929027 1105 88-89 0.0760034245204011 1105 90-91 0.06911030086972403 1105 92-93 0.030087123522712034 1105 94-95 0.003757339106595481 1105 96-97 -0.00892265321365926 1105 98-99 -0.05583638346103115 1105 100 0.048071860052466775 1106 1 -0.005332721855690892 1106 2 0.0561864164823227 1106 3 0.11493051271630605 1106 4 0.1136680241515009 1106 5 0.008382571530965777 1106 6 0.06700418106806438 1106 7 0.06107909615312934 1106 8 0.15790739844691615 1106 9 0.09911566246385206 1106 10-11 0.15077439358058342 1106 12-13 0.19014174051890365 1106 14-15 0.21775712863222907 1106 16-17 0.212754936416232 1106 18-19 0.16397472798050927 1106 20-21 0.19114875152654776 1106 22-23 0.15371429196194697 1106 24-25 0.17424316824965302 1106 26-27 0.2322259111891114 1106 28-29 0.21557194192049423 1106 30-31 0.14916619365248351 1106 32-33 0.1966464354492885 1106 34-35 0.1904772779671191 1106 36-37 0.3006145864797176 1106 38-39 0.27497616134122893 1106 40-41 0.30454654514656454 1106 42-43 0.3383527942219544 1106 44-45 0.29078098465569724 1106 46-47 0.29949457224527265 1106 48-49 0.28754363425736784 1106 50-51 0.31844402137002703 1106 52-53 0.360375307861176 1106 54-55 0.29132125743867476 1106 56-57 0.27271153025015593 1106 58-59 0.3292486932489851 1106 60-61 0.3493826856676421 1106 62-63 0.27331787050120226 1106 64-65 0.24790413071356454 1106 66-67 0.34563305523361265 1106 68-69 0.34111995619566926 1106 70-71 0.39106748305005823 1106 72-73 0.41398875814417835 1106 74-75 0.3137964995573981 1106 76-77 0.3350332698499372 1106 78-79 0.42646664696626146 1106 80-81 0.35939390090630496 1106 82-83 0.31106659012391447 1106 84-85 0.25194438347576664 1106 86-87 0.26593031580463133 1106 88-89 0.24678406524289898 1106 90-91 0.21060330400738536 1106 92-93 0.2524304269470363 1106 94-95 0.25368156017045607 1106 96-97 0.31513000904598343 1106 98-99 0.28814185972290574 1106 100 0.38253084242991164 1107 1 -0.02217701697160379 1107 2 0.07628633262872597 1107 3 0.10536322440179902 1107 4 0.03852467175050123 1107 5 0.019387465021722505 1107 6 -0.020198385261231522 1107 7 0.08937261454838108 1107 8 0.038618243335697855 1107 9 0.0780253449905075 1107 10-11 0.08224908333722425 1107 12-13 0.05107197740818492 1107 14-15 0.09339307204955816 1107 16-17 0.149400406310221 1107 18-19 0.14655452857587647 1107 20-21 0.0946244566313652 1107 22-23 0.1107135150458518 1107 24-25 0.10138595871429601 1107 26-27 0.08722977737560456 1107 28-29 0.11576380271846887 1107 30-31 0.07056132251042158 1107 32-33 0.03634759521326458 1107 34-35 0.0726822320660716 1107 36-37 0.13702856802640895 1107 38-39 0.09195821593597486 1107 40-41 0.09977920266175744 1107 42-43 0.14030443090757672 1107 44-45 0.1658592453293295 1107 46-47 0.1518094097332252 1107 48-49 0.17281653322125123 1107 50-51 0.1450565936152941 1107 52-53 0.13319052162115952 1107 54-55 0.1468017003751214 1107 56-57 0.12009723862680488 1107 58-59 0.15414930248088154 1107 60-61 0.2813839033496919 1107 62-63 0.2085844235390013 1107 64-65 0.1741949920971564 1107 66-67 0.19174394523364668 1107 68-69 0.06612695165948423 1107 70-71 0.06978485642526522 1107 72-73 0.10999998721440818 1107 74-75 0.030640357816082542 1107 76-77 0.0572461034737799 1107 78-79 0.11164692070099136 1107 80-81 0.13838133252377816 1107 82-83 0.04784407137526614 1107 84-85 0.03870494225756005 1107 86-87 0.05789321191055308 1107 88-89 0.035415235190249916 1107 90-91 0.008235342380928046 1107 92-93 0.060861576063189204 1107 94-95 0.09142818619560344 1107 96-97 0.043712738424193986 1107 98-99 -0.010694984907900107 1107 100 -0.018565830166515696 1108 1 -0.018148891741489592 1108 2 0.025857481911497615 1108 3 0.014007639524251658 1108 4 -0.026969513273925827 1108 5 -0.027282929916317755 1108 6 0.018428695389502536 1108 7 0.038907940583058576 1108 8 -0.08721953970942309 1108 9 -0.08703358756957869 1108 10-11 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-0.17700443388888942 2214 72-73 -0.2439481855982777 2214 74-75 -0.1463000456667629 2214 76-77 -0.11854017763469926 2214 78-79 -0.03161738489659882 2214 80-81 -0.14121482851323464 2214 82-83 -0.14092423165827483 2214 84-85 -0.08021239658375379 2214 86-87 -0.01808567023186214 2214 88-89 -0.05337829294018803 2214 90-91 -0.07953137592299697 2214 92-93 -0.12443901806202717 2214 94-95 -0.11346666966522889 2214 96-97 -0.12248859939624168 2214 98-99 -0.1926418282095952 2214 100 -0.1916159301766065 2215 1 -0.15434847034940447 2215 2 -0.2794318807612015 2215 3 -0.22938326825275723 2215 4 -0.23509593315748845 2215 5 -0.18795418763393457 2215 6 -0.3028647028011733 2215 7 -0.354512155257666 2215 8 -0.34076134469476926 2215 9 -0.4028961428405893 2215 10-11 -0.3820980726581027 2215 12-13 -0.4087230369703221 2215 14-15 -0.37521825343760185 2215 16-17 -0.4668811873613947 2215 18-19 -0.4116680948358251 2215 20-21 -0.4200578150811083 2215 22-23 -0.35160060942700966 2215 24-25 -0.4079540424800854 2215 26-27 -0.433060629448903 2215 28-29 -0.3864084775384171 2215 30-31 -0.39466783783980475 2215 32-33 -0.38914141188870843 2215 34-35 -0.43501865361329806 2215 36-37 -0.3544149561322456 2215 38-39 -0.442062977434297 2215 40-41 -0.4043870620497927 2215 42-43 -0.44279002624631403 2215 44-45 -0.443440388087609 2215 46-47 -0.4477706620214974 2215 48-49 -0.4114013884641068 2215 50-51 -0.37582296455565256 2215 52-53 -0.346186310145697 2215 54-55 -0.38569378624321615 2215 56-57 -0.30336304361458843 2215 58-59 -0.35246199774054077 2215 60-61 -0.2840050054455503 2215 62-63 -0.3308734998001128 2215 64-65 -0.27873348056530745 2215 66-67 -0.34922877464727975 2215 68-69 -0.34861750825066196 2215 70-71 -0.34054269105137536 2215 72-73 -0.38349666622219303 2215 74-75 -0.3162049836390466 2215 76-77 -0.2970491888279767 2215 78-79 -0.25180720588500094 2215 80-81 -0.27183596440850266 2215 82-83 -0.4100944846182273 2215 84-85 -0.3305528418902526 2215 86-87 -0.3399628169567208 2215 88-89 -0.291168984741212 2215 90-91 -0.28025444336551786 2215 92-93 -0.33014829595429873 2215 94-95 -0.33857047012588737 2215 96-97 -0.32079824298940096 2215 98-99 -0.3369401269932908 2215 100 -0.3398359164375684 2216 1 -0.10119906479727092 2216 2 -0.2512235743133786 2216 3 -0.3010935636101557 2216 4 -0.29709870576418496 2216 5 -0.2595467430057212 2216 6 -0.3305159847763903 2216 7 -0.3032102785873647 2216 8 -0.3749468868425936 2216 9 -0.4351709597100992 2216 10-11 -0.4669720692414785 2216 12-13 -0.4484263661997545 2216 14-15 -0.6350010247092968 2216 16-17 -0.5867508451799495 2216 18-19 -0.5532209152801855 2216 20-21 -0.552300678369221 2216 22-23 -0.5270212131210243 2216 24-25 -0.591689839514892 2216 26-27 -0.555309268485928 2216 28-29 -0.566981287203852 2216 30-31 -0.5835899885321538 2216 32-33 -0.5930485252380109 2216 34-35 -0.6384064440076926 2216 36-37 -0.7013911398980923 2216 38-39 -0.6415397761675266 2216 40-41 -0.7211866574692465 2216 42-43 -0.7559511934358554 2216 44-45 -0.6502332126853148 2216 46-47 -0.6014262104487855 2216 48-49 -0.6110915716069556 2216 50-51 -0.6436529239121853 2216 52-53 -0.6458294745515047 2216 54-55 -0.6452029897399427 2216 56-57 -0.6399428793595803 2216 58-59 -0.5525627256888583 2216 60-61 -0.5881131729540101 2216 62-63 -0.620636162565603 2216 64-65 -0.6295753832681896 2216 66-67 -0.6148942960530874 2216 68-69 -0.6854726114292049 2216 70-71 -0.7028846382544174 2216 72-73 -0.6541018590191072 2216 74-75 -0.6258863316475356 2216 76-77 -0.6082500316114121 2216 78-79 -0.559848846902284 2216 80-81 -0.6220009394513326 2216 82-83 -0.6003521806723029 2216 84-85 -0.5851793125584734 2216 86-87 -0.6070461626512014 2216 88-89 -0.6154833888223727 2216 90-91 -0.6324702882627093 2216 92-93 -0.6454589131961299 2216 94-95 -0.7032166908268636 2216 96-97 -0.6631866619484441 2216 98-99 -0.641790890788954 2216 100 -0.6389993135791734 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 26830.0 3 4238.0 4 2862.0 5 2415.0 6 1915.0 7 2053.0 8 2369.0 9 2544.0 10 2739.0 11 2905.0 12 2979.0 13 3481.0 14 3671.0 15 3942.0 16 4194.0 17 3894.0 18 3913.0 19 3710.0 20 3685.0 21 3759.0 22 4572.0 23 6585.0 24 10464.0 25 14085.0 26 14192.0 27 11687.0 28 12937.0 29 16264.0 30 19668.0 31 23796.0 32 30876.0 33 41769.0 34 61223.0 35 97571.0 36 189493.0 37 403098.0 38 636459.0 39 280069.0 40 546.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.359027813528506 19.03906799932922 12.791460466377524 23.810443720764756 2 15.800572321613348 19.744534960654164 40.016382629723005 24.438510088009487 3 18.764686752281833 25.140304679065473 29.236079889815585 26.858928678837106 4 12.392106088140018 16.381115984840182 37.99221619307104 33.234561733948766 5 13.261970481265978 38.22021046944013 34.85601270258767 13.661806346706223 6 31.54931027698004 37.40377469285891 17.652444028500327 13.394471001660719 7 28.440194880065572 32.25516891950442 21.663484250864755 17.641151949565252 8 25.7953033647156 36.571262545684384 19.575804223607552 18.057629865992467 9 25.377197686249005 17.318114256237525 20.37468983136205 36.929998226151426 10-11 24.40942072385943 27.062459062769996 28.043270492826906 20.48484972054367 12-13 24.887702489137247 24.371841174556145 28.24277619580761 22.497680140499 14-15 22.05986243933171 26.275932795690704 25.206916975296373 26.457287789681217 16-17 22.116674065854642 29.683214513078216 25.773308588953615 22.42680283211353 18-19 22.08574166386332 28.73171452745752 27.481192085384876 21.70135172329429 20-21 23.047331238648088 27.91333314134742 27.134719828415566 21.904615791588927 22-23 22.82331569402408 27.663302930925337 27.1791309063737 22.33425046867689 24-25 22.545529334018724 27.800755851040922 27.11323162382075 22.540483191119602 26-27 22.387750072569755 27.75199926805853 27.268770080923876 22.59148057844784 28-29 22.465309367808622 27.85525883225407 27.272965899331542 22.406465900605767 30-31 22.52817576601231 28.0625328447546 26.971258705293543 22.438032683939547 32-33 22.32781379569241 27.916985977181923 27.374274909214723 22.380925317910943 34-35 22.53248010927792 27.62965284892226 27.358904570406274 22.478962471393547 36-37 22.686213738504907 27.872212139143432 27.044575702855152 22.396998419496512 38-39 22.462078023882906 28.05571285058173 27.169673224467623 22.312535901067744 40-41 22.302842887226184 27.76595411205743 27.401389905814753 22.529813094901634 42-43 22.30502997186315 27.835399420951763 27.239581209476814 22.619989397708274 44-45 22.308273204264506 27.907279026149933 27.28013226483653 22.50431550474903 46-47 22.467494799320814 27.973231221100615 27.213557397096388 22.345716582482186 48-49 22.367852935494113 27.795755409729434 27.30154045196582 22.53485120281063 50-51 22.193270184870215 27.862919084139133 27.369539324459446 22.57427140653121 52-53 22.37014622250044 27.809955609206042 27.308494607650225 22.511403560643288 54-55 22.370140860004568 28.073589564777656 27.19538106423934 22.360888510978434 56-57 22.287451394595344 27.91247560809006 27.224277112487233 22.575795884827365 58-59 22.268487874403238 27.510751305538427 27.52538118612649 22.695379633931843 60-61 22.482745261955976 27.914470531863923 27.19193793391716 22.410846272262944 62-63 22.24004624390602 27.916801748623183 27.227756334579144 22.61539567289165 64-65 22.299051424138145 27.848289030947864 27.268178998390752 22.58448054652324 66-67 22.177614412151357 27.767818047710534 27.215082763742686 22.839484776395423 68-69 22.42026940647183 27.898775304644623 27.223984781276727 22.456970507606812 70-71 22.45914862979222 27.691794045123512 27.239208140327626 22.609849184756637 72-73 22.25398662836588 27.76458013002593 27.40387274290277 22.577560498705424 74-75 22.28622357151486 27.9841078654801 27.225793396134566 22.503875166870472 76-77 22.391337147424263 27.785129123153276 27.13408480167679 22.689448927745676 78-79 22.399764711459333 27.82179951387634 27.266302434976435 22.51213333968789 80-81 22.373719558941065 27.77912289787463 27.30683282496289 22.54032471822141 82-83 22.290944818173823 27.712100803712854 27.391991925916205 22.604962452197118 84-85 22.2294546339611 27.77924889676695 27.310609005032244 22.680687464239703 86-87 22.458993664591905 27.770103541028764 27.221840209477776 22.549062584901552 88-89 22.207647237223384 27.756494391873122 27.276879123903797 22.758979246999697 90-91 22.238914832518326 27.695792850567752 27.283905877124038 22.781386439789884 92-93 22.469631655200622 27.8402254896589 27.187559331839072 22.502583523301407 94-95 22.230142379336392 27.62719285279585 27.323335756102885 22.819329011764875 96-97 22.315318139304928 27.57068842047855 27.36515363502221 22.748839805194308 98-99 22.23505231355769 27.781270586796165 27.236995515980773 22.746681583665378 100 22.324975458490446 27.546379120705488 27.246223461785075 22.88242195901899 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2458.0 1 1678.5 2 803.0 3 610.5 4 473.0 5 388.5 6 330.5 7 319.5 8 317.5 9 313.5 10 308.0 11 292.5 12 283.0 13 297.5 14 338.5 15 375.0 16 417.5 17 539.5 18 695.0 19 902.0 20 1281.0 21 1745.0 22 2488.0 23 4038.0 24 6021.0 25 8178.0 26 11067.0 27 14864.0 28 19672.5 29 24591.0 30 29010.0 31 33464.0 32 37587.5 33 42039.5 34 46708.0 35 50271.5 36 53715.5 37 57348.5 38 60122.0 39 61673.0 40 63863.5 41 66219.0 42 67252.5 43 68208.0 44 67843.5 45 67262.5 46 68001.5 47 67832.5 48 67865.0 49 66761.0 50 64348.5 51 62060.5 52 58239.5 53 55932.5 54 54776.5 55 51965.5 56 48165.0 57 44313.5 58 40500.5 59 37133.5 60 33704.0 61 29469.5 62 25905.0 63 22659.0 64 18985.0 65 15511.5 66 12438.5 67 9698.0 68 7386.5 69 5675.0 70 4264.5 71 3151.0 72 2397.0 73 1817.5 74 1440.0 75 1072.5 76 709.5 77 518.0 78 383.0 79 247.0 80 174.0 81 117.5 82 83.0 83 65.0 84 48.0 85 42.5 86 36.5 87 29.5 88 20.5 89 14.0 90 11.5 91 7.0 92 5.5 93 5.0 94 3.0 95 5.5 96 5.5 97 1.5 98 0.5 99 0.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0797065576342075 2 0.08240588514514233 3 0.08403566779325392 4 0.08592010398013296 5 0.08388287566999346 6 0.08403566779325392 7 0.08301705363818418 8 0.08260960797615628 9 0.08230402372963536 10-11 0.08184564735985397 12-13 0.08085249855866097 14-15 0.07914631984891915 16-17 0.07766932932406802 18-19 0.07700723012327268 20-21 0.07652338839961455 22-23 0.07873887418689125 24-25 0.07932457732605636 26-27 0.078764339540768 28-29 0.07471534827436577 30-31 0.08001214188072843 32-33 0.07856061670975405 34-35 0.07547930889066806 36-37 0.07227067430219837 38-39 0.07451162544335181 40-41 0.07744014113917733 42-43 0.0821003008986214 44-45 0.08304251899206092 46-47 0.08602196539563992 48-49 0.08938339210737008 50-51 0.09058026373957703 52-53 0.09086038263222121 54-55 0.09106410546323516 56-57 0.08841570866005383 58-59 0.08726976773560036 60-61 0.08515614336383064 62-63 0.08556358902585853 64-65 0.08500335124057018 66-67 0.08469776699404925 68-69 0.08444311345528183 70-71 0.08222762766800513 72-73 0.08212576625249815 74-75 0.08154006311333305 76-77 0.0816928552365935 78-79 0.08176925129822374 80-81 0.08291519222267721 82-83 0.08248228120677256 84-85 0.08171832059047025 86-87 0.08255867726840278 88-89 0.07980841904971449 90-91 0.07670164587675177 92-93 0.07637059627635409 94-95 0.07690536870776571 96-97 0.07624326950697038 98-99 0.0756066356600518 100 0.07486814039762622 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1963452.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.30249488918243 #Duplication Level Percentage of deduplicated Percentage of total 1 79.17766794976716 50.91321588677287 2 12.517180875538472 16.09771918552569 3 3.657186751284272 7.054986971497278 4 1.5693638002461676 4.0365603097838845 5 0.8088834302974133 2.600661132132189 6 0.5205390228269108 2.0083174712968472 7 0.339300593954036 1.5272512296038172 8 0.25055773042742346 1.2889189744203635 9 0.17867159995641374 1.0340126678735408 >10 0.9287239429955105 10.422332234720049 >50 0.041847427225925216 1.8145780220553451 >100 0.00968048856137118 0.940187069057532 >500 1.5855476758239775E-4 0.06290603220519668 >1k 2.3783215137359654E-4 0.1983528130554459 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 5.093070775348723E-5 0.0 8 0.0 0.0 0.0 5.093070775348723E-5 0.0 9 0.0 0.0 0.0 1.0186141550697446E-4 0.0 10-11 0.0 0.0 0.0 1.0186141550697446E-4 0.0 12-13 2.5465353876743614E-5 0.0 0.0 1.0186141550697446E-4 0.0 14-15 5.093070775348723E-5 0.0 0.0 1.0186141550697446E-4 0.0 16-17 5.093070775348723E-5 0.0 0.0 1.0186141550697446E-4 0.0 18-19 5.093070775348723E-5 0.0 0.0 1.0186141550697446E-4 0.0 20-21 5.093070775348723E-5 0.0 0.0 1.2732676938371807E-4 0.0 22-23 1.0186141550697446E-4 0.0 0.0 1.527921232604617E-4 0.0 24-25 1.527921232604617E-4 0.0 0.0 1.527921232604617E-4 0.0 26-27 1.527921232604617E-4 0.0 0.0 2.5465353876743613E-4 0.0 28-29 1.527921232604617E-4 0.0 0.0 8.912873856860265E-4 0.0 30-31 1.527921232604617E-4 0.0 0.0 0.004481902282306876 0.0 32-33 1.527921232604617E-4 5.093070775348723E-5 0.0 0.011612201367795089 0.0 34-35 1.527921232604617E-4 5.093070775348723E-5 0.0 0.021543689379725096 0.0 36-37 1.527921232604617E-4 5.093070775348723E-5 0.0 0.040770031556666524 0.0 38-39 1.527921232604617E-4 5.093070775348723E-5 0.0 0.07700723012327268 0.0 40-41 1.527921232604617E-4 5.093070775348723E-5 0.0 0.12307405528630187 0.0 42-43 1.527921232604617E-4 5.093070775348723E-5 0.0 0.16850424660241248 0.0 44-45 1.527921232604617E-4 5.093070775348723E-5 0.0 0.21938402364814624 0.0 46-47 1.527921232604617E-4 5.093070775348723E-5 0.0 0.2821306556004425 0.0 48-49 1.527921232604617E-4 5.093070775348723E-5 0.0 0.35549634011934084 0.0 50-51 1.527921232604617E-4 5.093070775348723E-5 0.0 0.441492840161104 0.0 52-53 1.527921232604617E-4 5.093070775348723E-5 0.0 0.5271837559563463 0.0 54-55 1.527921232604617E-4 5.093070775348723E-5 0.0 0.6181205346501977 0.0 56-57 1.527921232604617E-4 5.093070775348723E-5 0.0 0.723776287884807 0.0 58-59 1.782574771372053E-4 5.093070775348723E-5 0.0 0.8455261447695182 0.0 60-61 2.037228310139489E-4 5.093070775348723E-5 0.0 0.9811546195170546 0.0 62-63 2.037228310139489E-4 5.093070775348723E-5 0.0 1.1193296296522655 0.0 64-65 2.037228310139489E-4 5.093070775348723E-5 0.0 1.2623430570240577 0.0 66-67 2.037228310139489E-4 5.093070775348723E-5 0.0 1.4244300344495309 0.0 68-69 2.037228310139489E-4 5.093070775348723E-5 0.0 1.6167189215728217 0.0 70-71 2.037228310139489E-4 5.093070775348723E-5 0.0 1.826960883179217 0.0 72-73 2.037228310139489E-4 5.093070775348723E-5 0.0 2.0441294210400867 0.0 74-75 2.037228310139489E-4 5.093070775348723E-5 0.0 2.2570961755112933 0.0 76-77 2.037228310139489E-4 5.093070775348723E-5 0.0 2.488958222559044 0.0 78-79 2.037228310139489E-4 5.093070775348723E-5 0.0 2.752168120229066 0.0 80-81 2.037228310139489E-4 5.093070775348723E-5 0.0 3.041301748145613 0.0 82-83 2.037228310139489E-4 5.093070775348723E-5 0.0 3.3532268677818458 0.0 84-85 2.037228310139489E-4 5.093070775348723E-5 0.0 3.65371804352742 0.0 86-87 2.037228310139489E-4 5.093070775348723E-5 0.0 3.9645736183008298 0.0 88 2.037228310139489E-4 5.093070775348723E-5 0.0 4.212835353245203 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 6720 0.0 29.516146 1 TACATGG 6735 0.0 28.124443 2 ACATGGG 6975 0.0 26.145256 3 GTATCAA 3735 0.0 25.420097 1 TCAACGC 3800 0.0 23.995153 4 CAACGCA 3950 0.0 23.32133 5 ATCAACG 3940 0.0 23.142532 3 AACGCAG 4060 0.0 22.57371 6 ACACCGT 355 0.0 22.506859 6 TACCTGG 1400 0.0 21.822365 2 TATCAAC 4265 0.0 21.820395 2 CATGGGG 4730 0.0 21.56274 4 GGTATCA 2695 0.0 21.102943 1 TAACCCG 120 0.0050656223 19.583174 5 ACGCAGA 4755 0.0 19.274292 7 GAGTACA 4745 0.0 19.216843 1 GTACCTG 1730 0.0 19.018156 1 CGCGCGT 150 8.2937256E-4 18.799849 7 CGCAGAG 5055 0.0 18.03744 8 AGTACAT 4805 0.0 17.99869 2 >>END_MODULE